(Circular) (Six-base) MAPSORT of: pFN21AB8153.seq Check: 5244 from: 1 to: 5944 pFN21AB8153 5944 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3754 278 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3287 1937 Size: 1350 4594 Acc65I G'GTAC_C Cuts at: 3265 3265 Size: 5944 AclI AA'CG_TT Cuts at: 1563 4071 4444 1563 Size: 2508 373 3063 Fragments arranged by size: 3063 2508 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2712 4007 5391 5659 1632 Size: 1080 1295 1384 268 1917 Fragments arranged by size: 1917 1384 1295 1080 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5747 AflIII A'CryG_T Cuts at: 1203 4843 5820 1203 Size: 3640 977 1327 Fragments arranged by size: 3640 1327 977 AgeI A'CCGG_T Cuts at: 4775 4775 Size: 5944 AhdI GACnn_n'nnGTC Cuts at: 2107 4673 2107 Size: 2566 3378 AleI CACnn'nnGTG Cuts at: 1166 2786 1166 Size: 1620 4324 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3216 1184 Size: 2032 3912 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2700 2732 2700 Size: 32 5912 AlwNI CAG_nnn'CTG Cuts at: 2098 2161 3687 5259 2098 Size: 63 1526 1572 2783 Fragments arranged by size: 2783 1572 1526 63 ApaLI G'TGCA_C Cuts at: 4001 5157 4001 Size: 1156 4788 ApoI r'AATT_y Cuts at: 1493 2648 3253 3479 3631 5740 1493 Size: 1155 605 226 152 2109 1697 Fragments arranged by size: 2109 1697 1155 605 226 152 AseI AT'TA_AT Cuts at: 160 1032 3081 4498 160 Size: 872 2049 1417 1606 Fragments arranged by size: 2049 1606 1417 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 AvaI C'yCGr_G Cuts at: 1870 1943 2239 2330 2566 3129 3269 1870 Size: 73 296 91 236 563 140 4545 Fragments arranged by size: 4545 563 296 236 140 91 73 BamHI G'GATC_C Cuts at: 3274 3641 3274 Size: 367 5577 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2042 2148 2302 2347 2804 Size: 325 217 627 255 106 154 45 457 Cuts at: 2804 3125 3265 4720 618 Size: 321 140 1455 1842 Fragments arranged by size: 1842 1455 627 457 325 321 255 217 154 140 106 45 BanII G_rGCy'C Cuts at: 1391 2228 3263 1391 Size: 837 1035 4072 Fragments arranged by size: 4072 1035 837 BbeI G_GCGC'C Cuts at: 2046 2152 2306 2351 2046 Size: 106 154 45 5639 Fragments arranged by size: 5639 154 106 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2700 928 Size: 971 801 4172 Fragments arranged by size: 4172 971 801 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4135 4169 1903 Size: 34 2198 34 3678 Fragments arranged by size: 3678 2198 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3836 5046 3836 Size: 1210 4734 BclI T'GATC_A Cuts at: 1548 1548 Size: 5944 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2334 4555 136 Size: 107 122 71 631 605 662 2221 1525 Fragments arranged by size: 2221 1525 662 631 605 122 107 71 BglII A'GATC_T Cuts at: 5939 5939 Size: 5944 BlpI GC'TnA_GC Cuts at: 2313 2433 3361 2313 Size: 120 928 4896 Fragments arranged by size: 4896 928 120 Bme1580I G_kGCm'C Cuts at: 1790 2807 4005 5161 1790 Size: 1017 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 1017 BmgBI CAC'GTC Cuts at: 1318 2558 1318 Size: 1240 4704 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3675 4633 442 Size: 416 53 83 399 24 2258 958 1753 Fragments arranged by size: 2258 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5944 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2808 3095 4604 1239 Size: 1569 287 1509 2579 Fragments arranged by size: 2579 1569 1509 287 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4069 4934 5232 4069 Size: 865 298 4781 Fragments arranged by size: 4781 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4607 882 Size: 617 3108 2219 Fragments arranged by size: 3108 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3735 493 Size: 711 2531 2702 Fragments arranged by size: 2702 2531 711 BsaBI GATnn'nnATC Cuts at: 5938 5938 Size: 5944 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2043 2149 2303 2348 Size: 53 83 186 936 510 106 154 45 Cuts at: 2348 3751 4133 4768 275 Size: 1403 382 635 1451 Fragments arranged by size: 1451 1403 936 635 510 382 186 154 106 83 53 45 BsaWI w'CCGG_w Cuts at: 2937 3042 4377 4775 5049 5196 5607 2937 Size: 105 1335 398 274 147 411 3274 Fragments arranged by size: 3274 1335 411 398 274 147 105 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5719 Fragments arranged by size: 5719 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2807 2831 3135 1186 Size: 1621 24 304 3995 Fragments arranged by size: 3995 1621 304 24 BseYI C'CCAG_C Cuts at: 2227 3069 5147 5643 2227 Size: 842 2078 496 2528 Fragments arranged by size: 2528 2078 842 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2748 1163 Size: 573 1012 4359 Fragments arranged by size: 4359 1012 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2199 2266 3394 4155 5183 1540 Size: 275 186 198 67 1128 761 1028 2301 Fragments arranged by size: 2301 1128 1028 761 275 198 186 67 BsiHKAI G_wGCw'C Cuts at: 4005 4090 5161 4005 Size: 85 1071 4788 Fragments arranged by size: 4788 1071 85 BsmI GAATG_Cn' Cuts at: 3455 3548 3455 Size: 93 5851 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2807 3263 4005 4090 5161 1391 Size: 399 438 579 456 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 579 456 438 399 85 BspEI T'CCGG_A Cuts at: 2937 3042 5607 2937 Size: 105 2565 3274 Fragments arranged by size: 3274 2565 105 BspHI T'CATG_A Cuts at: 3833 5563 3833 Size: 1730 4214 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3299 844 Size: 2455 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2437 3831 1394 Size: 429 614 1394 3507 Fragments arranged by size: 3507 1394 614 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4439 4613 65 Size: 1183 3191 174 1396 Fragments arranged by size: 3191 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2145 2204 2252 4588 4775 1700 Size: 110 335 59 48 2336 187 2869 Fragments arranged by size: 2869 2336 335 187 110 59 48 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5944 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5944 BssSI C'ACGA_G Cuts at: 4004 5016 4004 Size: 1012 4932 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 794 Size: 1367 4577 BstBI TT'CG_AA Cuts at: 2719 4748 2719 Size: 2029 3915 BstEII G'GTnAC_C Cuts at: 1184 3089 1184 Size: 1905 4039 BstXI CCAn_nnnn'nTGG Cuts at: 2752 2788 2971 2752 Size: 36 183 5725 Fragments arranged by size: 5725 183 36 BstYI r'GATC_y Cuts at: 1972 2441 3274 3641 4026 4043 5495 5581 5593 Size: 469 833 367 385 17 1452 86 12 Cuts at: 5593 5939 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 833 469 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2028 2212 2404 2970 513 Size: 552 963 184 192 566 3487 Fragments arranged by size: 3487 963 566 552 192 184 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2063 487 Size: 683 56 837 4368 Fragments arranged by size: 4368 837 683 56 BtsI GCAGTG_nn' Cuts at: 3460 4254 4274 3460 Size: 794 20 5130 Fragments arranged by size: 5130 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5944 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2686 2721 529 Size: 35 2122 35 3752 Fragments arranged by size: 3752 2122 35 35 DraI TTT'AAA Cuts at: 3248 3248 Size: 5944 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4951 809 Size: 507 3635 1802 Fragments arranged by size: 3635 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2076 2196 2263 Size: 54 1425 50 275 18 246 120 67 Cuts at: 2263 3391 4280 5909 8 Size: 1128 889 1629 43 Fragments arranged by size: 1629 1425 1128 889 275 246 120 67 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2196 2263 3391 1537 Size: 275 384 67 1128 4090 Fragments arranged by size: 4090 1128 384 275 67 EarI CTCTTCn'nnn_ Cuts at: 1353 2368 2808 3874 1353 Size: 1015 440 1066 3423 Fragments arranged by size: 3423 1066 1015 440 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4531 4905 5051 387 Size: 1064 138 2942 374 146 1280 Fragments arranged by size: 2942 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2712 2808 3095 4007 4604 5391 5659 Size: 393 1080 96 287 912 597 787 268 Cuts at: 5659 1239 Size: 1524 Fragments arranged by size: 1524 1080 912 787 597 393 287 268 96 EcoNI CCTnn'n_nnAGG Cuts at: 2132 2373 2132 Size: 241 5703 EcoO109I rG'GnC_Cy Cuts at: 2235 2235 Size: 5944 EcoRI G'AATT_C Cuts at: 3253 3631 3253 Size: 378 5566 FspI TGC'GCA Cuts at: 4450 4450 Size: 5944 HaeII r_GCGC'y Cuts at: 1678 2016 2046 2146 2152 2306 2351 5091 1678 Size: 338 30 100 6 154 45 2740 2531 Fragments arranged by size: 2740 2531 338 154 100 45 30 6 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2093 2125 2426 2458 3050 Size: 32 135 32 521 32 301 32 592 Cuts at: 3050 3082 4585 4617 4659 4691 1373 Size: 32 1503 32 42 32 2626 Fragments arranged by size: 2626 1503 592 521 301 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3288 3534 669 Size: 988 281 1350 246 3079 Fragments arranged by size: 3079 1350 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5944 HpaI GTT'AAC Cuts at: 3534 3534 Size: 5944 KasI G'GCGC_C Cuts at: 2042 2148 2302 2347 2042 Size: 106 154 45 5639 Fragments arranged by size: 5639 154 106 45 KpnI G_GTAC'C Cuts at: 3269 3269 Size: 5944 MfeI C'AATT_G Cuts at: 3543 3543 Size: 5944 MluI A'CGCG_T Cuts at: 5820 5820 Size: 5944 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2091 2823 5058 5242 1305 Size: 786 732 2235 184 2007 Fragments arranged by size: 2235 2007 786 732 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2078 10 Size: 54 1425 343 246 3876 Fragments arranged by size: 3876 1425 343 246 54 MslI CAynn'nnrTG Cuts at: 518 1166 2786 3902 4261 4420 518 Size: 648 1620 1116 359 159 2042 Fragments arranged by size: 2042 1620 1116 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2039 2095 2161 2214 2317 2495 3073 3360 Size: 433 56 66 53 103 178 578 287 Cuts at: 3360 4037 5185 5430 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 578 433 287 245 178 103 66 56 53 NaeI GCC'GGC Cuts at: 2147 2254 2147 Size: 107 5837 NarI GG'CG_CC Cuts at: 2043 2149 2303 2348 2043 Size: 106 154 45 5639 Fragments arranged by size: 5639 154 106 45 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5392 NdeI CA'TA_TG Cuts at: 387 387 Size: 5944 NgoMIV G'CCGG_C Cuts at: 2145 2252 2145 Size: 107 5837 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5944 NotI GC'GGCC_GC Cuts at: 3391 3391 Size: 5944 NspI r_CATG'y Cuts at: 1132 1229 3302 4847 1132 Size: 97 2073 1545 2229 Fragments arranged by size: 2229 2073 1545 97 PasI CC'CwG_GG Cuts at: 1348 2603 2800 1348 Size: 1255 197 4492 Fragments arranged by size: 4492 1255 197 PciI A'CATG_T Cuts at: 4843 4843 Size: 5944 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5944 PmeI GTTT'AAAC Cuts at: 3248 3248 Size: 5944 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4013 4045 5526 5558 4013 Size: 32 1481 32 4399 Fragments arranged by size: 4399 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5944 PsiI TTA'TAA Cuts at: 3514 3514 Size: 5944 PstI C_TGCA'G Cuts at: 830 2166 2346 3296 830 Size: 1336 180 950 3478 Fragments arranged by size: 3478 1336 950 180 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5944 PvuII CAG'CTG Cuts at: 2095 2161 2495 3073 2095 Size: 66 334 578 4966 Fragments arranged by size: 4966 578 334 66 SacII CC_GC'GG Cuts at: 2215 2215 Size: 5944 SalI G'TCGA_C Cuts at: 1936 3286 1936 Size: 1350 4594 SbfI CC_TGCA'GG Cuts at: 2346 3296 2346 Size: 950 4994 ScaI AGT'ACT Cuts at: 4192 5904 4192 Size: 1712 4232 SexAI A'CCwGG_T Cuts at: 2518 2518 Size: 5944 SfcI C'TryA_G Cuts at: 826 1045 2162 2342 2446 3292 4427 5108 5299 Size: 219 1117 180 104 846 1135 681 191 Cuts at: 5299 826 Size: 1471 Fragments arranged by size: 1471 1135 1117 846 681 219 191 180 104 SfoI GGC'GCC Cuts at: 2044 2150 2304 2349 2044 Size: 106 154 45 5639 Fragments arranged by size: 5639 154 106 45 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5944 SgrAI Cr'CCGG_yG Cuts at: 1700 2145 1700 Size: 445 5499 SmaI CCC'GGG Cuts at: 1872 2332 3131 3271 1872 Size: 460 799 140 4545 Fragments arranged by size: 4545 799 460 140 SmlI C'TyrA_G Cuts at: 820 1017 1943 4048 4949 5211 820 Size: 197 926 2105 901 262 1553 Fragments arranged by size: 2105 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3735 493 Size: 3242 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5944 SphI G_CATG'C Cuts at: 1132 3302 1132 Size: 2170 3774 SspI AAT'ATT Cuts at: 5 52 3868 5 Size: 47 3816 2081 Fragments arranged by size: 3816 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5944 StyI C'CwwG_G Cuts at: 513 1065 3383 513 Size: 552 2318 3074 Fragments arranged by size: 3074 2318 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2693 3971 3988 4141 4326 4771 325 Size: 1255 1113 1278 17 153 185 445 1498 Fragments arranged by size: 1498 1278 1255 1113 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4190 5902 96 Size: 274 80 33 51 1443 2213 1712 138 Fragments arranged by size: 2213 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5904 5936 5904 Size: 32 5912 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5944 XbaI T'CTAG_A Cuts at: 3280 3280 Size: 5944 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3181 3181 Size: 5944 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5944 XmaI C'CCGG_G Cuts at: 1870 2330 3129 3269 1870 Size: 460 799 140 4545 Fragments arranged by size: 4545 799 460 140 XmnI GAAnn'nnTTC Cuts at: 4073 4073 Size: 5944 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3752 276 Size: 53 83 186 936 2218 2468 Fragments arranged by size: 2468 2218 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SapI SfiI SrfI SwaI