Restriction map for
pFN21AB8147
##################################
# NO CUT ENZYMES ( 162 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrFI BsrGI
BssHII BstAPI BstBI BstEII BstPI
BstUI BstXI BstYI CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRV EcoT14I
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII HphI
KasI KpnI KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AfaI 210 GTAC GT^AC
AlwI 68 GGATC GGATC(4/5)
AlwNI 119 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 517 CCTAAGG CC^T[ACGT]AGG
ApaI 777 GGGCCC GGGCC^C
AspHI 114 GTGCTC G[AT]GC[AT]^C
AspI 389 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 777 GGGCCC G[GA]GC[CT]^C
BbsI 622 GAAGAC GAAGAC(2/6)
BlpI 193 GCTAAGC GC^T[ACGT]AGC
BpmI 428 CTCCAG CTGGAG(16/14)
Bpu1102I 193 GCTAAGC GC^T[ACGT]AGC
BpuAI 622 GAAGAC GAAGAC(2/6)
BseRI 787 GAGGAG GAGGAG(10/8)
BsgI 470 GTGCAG GTGCAG(16/14)
BsiHKAI 114 GTGCTC G[AT]GC[AT]^C
BsmBI 566 GAGACG CGTCTC(1/5)
BsrDI 165 CATTGC GCAATG(2/0)
BsrI 32 CCAGT ACTGG(1/-1)
BssSI 205 CACGAG CACGAG(-5/-1)
Bst1107I 13 GTATAC GTA^TAC
BstF5I 213 CATCC GGATG(2/0)
BstZ17I 13 GTATAC GTA^TAC
Bsu36I 517 CCTAAGG CC^T[ACGT]AGG
CelII 193 GCTAAGC GC^T[ACGT]AGC
DraII 776 GGGGCCC [GA]G^G[ACGT]CC[CT]
EarI 796 GAAGAG CTCTTC(1/4)
Eco81I 517 CCTAAGG CC^T[ACGT]AGG
EcoNI 766 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 776 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 316 GAATTC G^AATTC
FokI 213 CATCC GGATG(9/13)
Ksp632I 796 GAAGAG CTCTTC(1/4)
MaeIII 28 GTGAC ^GT[ACGT]AC
MseI 245 TTAA T^TAA
MwoI 514 GCTCCTAAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflFI 389 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 76 GAGTC GAGTC(4/5)
PvuII 578 CAGCTG CAG^CTG
RsaI 210 GTAC GT^AC
SalI 47 GTCGAC G^TCGAC
TfiI 90 GAATC G^A[AT]TC
Tru9I 245 TTAA T^TAA
Tsp45I 28 GTGAC ^GT[GC]AC
Tth111I 389 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 506 CCGC CCGC(-3/-1)
511 GCGG
AcsI 184 AAATTC [GA]^AATT[CT]
316 GAATTC
ApoI 184 AAATTC [GA]^AATT[CT]
316 GAATTC
Asp700I 316 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
336 GAAGTTTTTC
BfaI 199 CTAG C^TAG
464 CTAG
BmyI 114 GTGCTC G[AGT]GC[ACT]^C
777 GGGCCC
BsmAI 566 GAGAC GTCTC(1/5)
78 GTCTC
Bsp1286I 114 GTGCTC G[AGT]GC[ACT]^C
777 GGGCCC
BspMI 238 GCAGGT ACCTGC(4/8)
594 GCAGGT
Cac8I 60 GCTGGC GC[ACGT]^[ACGT]GC
523 GCTGGC
DpnII 69 GATC ^GATC
102 GATC
HinfI 76 GAGTC G^A[ACGT]TC
90 GAATC
MaeI 199 CTAG C^TAG
464 CTAG
MboI 69 GATC ^GATC
102 GATC
MspA1I 506 CCGCTG C[AC]G^C[GT]G
578 CAGCTG
NdeII 69 GATC ^GATC
102 GATC
NspI 56 ACATGC [GA]CATG^[CT]
146 ACATGC
PstI 82 CTGCAG CTGCA^G
404 CTGCAG
Sau3AI 69 GATC ^GATC
102 GATC
SfaNI 346 GATGC GCATC(5/9)
588 GATGC
SfcI 82 CTGCAG C^T[GA][CT]AG
404 CTGCAG
XmnI 316 GAATTCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
336 GAAGTTTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AccI AluI BbvI BsaJI BsiYI
BslI BssKI BstNI Cfr13I DdeI
EcoRII Fnu4HI HaeIII HincII HindII
ItaI MaeII MboII MnlI MvaI
NlaIII NlaIV Sau96I ScrFI TaqI
TseI Tsp509I TspRI TthHB8I