Restriction map for
pFN21AB8147
(Circular) (Six-base) MAPSORT of: pFN21AB8147.seq Check: 359 from: 1 to: 5509
pFN21AB8147 5509 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3319 278
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2019 2053 2733 2852 1937
Size: 82 34 680 119 4594
Fragments arranged by size:
4594 680 119 82 34
Acc65I G'GTAC_C
Cuts at: 2830 2830
Size: 5509
AclI AA'CG_TT
Cuts at: 1563 3636 4009 1563
Size: 2073 373 3063
Fragments arranged by size:
3063 2073 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3572 4956 5224 1632
Size: 1940 1384 268 1917
Fragments arranged by size:
1940 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5312
AflIII A'CryG_T
Cuts at: 1203 4408 5385 1203
Size: 3205 977 1327
Fragments arranged by size:
3205 1327 977
AgeI A'CCGG_T
Cuts at: 4340 4340
Size: 5509
AhdI GACnn_n'nnGTC
Cuts at: 4238 4238
Size: 5509
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5509
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5509
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2141 2173 2141
Size: 32 5477
AlwNI CAG_nnn'CTG
Cuts at: 2129 3252 4824 2129
Size: 1123 1572 2814
Fragments arranged by size:
2814 1572 1123
ApaI G_GGCC'C
Cuts at: 2786 2786
Size: 5509
ApaLI G'TGCA_C
Cuts at: 3566 4722 3566
Size: 1156 4353
ApoI r'AATT_y
Cuts at: 1493 2189 2321 2818 3044 3196 5305 1493
Size: 696 132 497 226 152 2109 1697
Fragments arranged by size:
2109 1697 696 497 226 152 132
AseI AT'TA_AT
Cuts at: 160 1032 4063 160
Size: 872 3031 1606
Fragments arranged by size:
3031 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
AvaI C'yCGr_G
Cuts at: 1870 1943 2834 1870
Size: 73 891 4545
Fragments arranged by size:
4545 891 73
BamHI G'GATC_C
Cuts at: 2839 3206 2839
Size: 367 5142
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2830 4285 618
Size: 325 217 627 1043 1455 1842
Fragments arranged by size:
1842 1455 1043 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2786 2828 1391
Size: 1395 42 4072
Fragments arranged by size:
4072 1395 42
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2634 928
Size: 971 735 3803
Fragments arranged by size:
3803 971 735
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3700 3734 1903
Size: 34 1763 34 3678
Fragments arranged by size:
3678 1763 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3401 4611 3401
Size: 1210 4299
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5509
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4120 136
Size: 107 122 71 631 605 2448 1525
Fragments arranged by size:
2448 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5504 5504
Size: 5509
BlpI GC'TnA_GC
Cuts at: 2199 2926 2199
Size: 727 4782
Bme1580I G_kGCm'C
Cuts at: 1790 2786 3570 4726 1790
Size: 996 784 1156 2573
Fragments arranged by size:
2573 1156 996 784
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5509
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3240 4198 442
Size: 416 53 83 399 24 1823 958 1753
Fragments arranged by size:
1823 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5509
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2418 4169 1239
Size: 1179 1751 2579
Fragments arranged by size:
2579 1751 1179
Bpu10I CC'TnA_GC
Cuts at: 2122 2122
Size: 5509
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3634 4499 4797 3634
Size: 865 298 4346
Fragments arranged by size:
4346 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4172 882
Size: 617 2673 2219
Fragments arranged by size:
2673 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3300 493
Size: 711 2096 2702
Fragments arranged by size:
2702 2096 711
BsaBI GATnn'nnATC
Cuts at: 5503 5503
Size: 5509
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3316 3698 4333 275
Size: 53 83 186 936 1783 382 635 1451
Fragments arranged by size:
1783 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3942 4340 4614 4761 5172 3942
Size: 398 274 147 411 4279
Fragments arranged by size:
4279 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5284
Fragments arranged by size:
5284 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2807 1186
Size: 1621 3888
BseYI C'CCAG_C
Cuts at: 4712 5208 4712
Size: 496 5013
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2496 1163
Size: 573 760 4176
Fragments arranged by size:
4176 760 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2959 3720 4748 1540
Size: 275 186 958 761 1028 2301
Fragments arranged by size:
2301 1028 958 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2123 3570 3655 4726 2123
Size: 1447 85 1071 2906
Fragments arranged by size:
2906 1447 1071 85
BsmI GAATG_Cn'
Cuts at: 3020 3113 3020
Size: 93 5416
BsmBI CGTCTCn'nnnn_
Cuts at: 2565 2565
Size: 5509
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2123 2786 2828 3570 3655 4726 1391
Size: 399 333 663 42 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 663 399 333 85 42
BspEI T'CCGG_A
Cuts at: 5172 5172
Size: 5509
BspHI T'CATG_A
Cuts at: 3398 5128 3398
Size: 1730 3779
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2234 2590 2864 844
Size: 1390 356 274 3489
Fragments arranged by size:
3489 1390 356 274
BsrBI CCG'CTC
Cuts at: 1394 1823 3396 1394
Size: 429 1573 3507
Fragments arranged by size:
3507 1573 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2169 4004 4178 65
Size: 1183 921 1835 174 1396
Fragments arranged by size:
1835 1396 1183 921 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4153 4340 1700
Size: 110 2343 187 2869
Fragments arranged by size:
2869 2343 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5509
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5509
BssSI C'ACGA_G
Cuts at: 2210 3569 4581 2210
Size: 1359 1012 3138
Fragments arranged by size:
3138 1359 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5509
BstBI TT'CG_AA
Cuts at: 4313 4313
Size: 5509
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5509
BstYI r'GATC_y
Cuts at: 1972 2839 3206 3591 3608 5060 5146 5158 5504
Size: 867 367 385 17 1452 86 12 346
Cuts at: 5504 1972
Size: 1977
Fragments arranged by size:
1977 1452 867 385 367 346 86 17
12
BstZ17I GTA'TAC
Cuts at: 2020 2020
Size: 5509
Bsu36I CC'TnA_GG
Cuts at: 2523 2523
Size: 5509
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4018
Fragments arranged by size:
4018 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4770
Fragments arranged by size:
4770 683 56
BtsI GCAGTG_nn'
Cuts at: 2016 2418 2479 3025 3819 3839 2016
Size: 402 61 546 794 20 3686
Fragments arranged by size:
3686 794 546 402 61 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5509
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5474
DraI TTT'AAA
Cuts at: 2813 2813
Size: 5509
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4516 809
Size: 507 3200 1802
Fragments arranged by size:
3200 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2956 3845 5474
Size: 54 1425 50 275 18 1126 889 1629
Cuts at: 5474 8
Size: 43
Fragments arranged by size:
1629 1425 1126 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2956 1537
Size: 275 1144 4090
Fragments arranged by size:
4090 1144 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2796 3439 1353
Size: 1443 643 3423
Fragments arranged by size:
3423 1443 643
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4096 4470 4616 387
Size: 1064 138 2507 374 146 1280
Fragments arranged by size:
2507 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2418 3572 4169 4956 5224 1239
Size: 393 786 1154 597 787 268 1524
Fragments arranged by size:
1524 1154 787 786 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2775 2775
Size: 5509
EcoO109I rG'GnC_Cy
Cuts at: 2782 2782
Size: 5509
EcoRI G'AATT_C
Cuts at: 2321 2818 3196 2321
Size: 497 378 4634
Fragments arranged by size:
4634 497 378
FspI TGC'GCA
Cuts at: 4015 4015
Size: 5509
HaeII r_GCGC'y
Cuts at: 1678 4656 1678
Size: 2978 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4150 4182 4224 4256 1373
Size: 32 135 32 2578 32 42 32 2626
Fragments arranged by size:
2626 2578 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2054 2266 2605 2853 3099 669
Size: 988 281 116 212 339 248 246 3079
Fragments arranged by size:
3079 988 339 281 248 246 212 116
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5509
HpaI GTT'AAC
Cuts at: 3099 3099
Size: 5509
KpnI G_GTAC'C
Cuts at: 2834 2834
Size: 5509
MfeI C'AATT_G
Cuts at: 3108 3108
Size: 5509
MluI A'CGCG_T
Cuts at: 5385 5385
Size: 5509
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4623 4807 1305
Size: 3318 184 2007
Fragments arranged by size:
3318 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3687
Fragments arranged by size:
3687 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2009 3467 3826 3985 518
Size: 648 843 1458 359 159 2042
Fragments arranged by size:
2042 1458 843 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2513 2585 2925 3602 4750 4995 1606
Size: 907 72 340 677 1148 245 2120
Fragments arranged by size:
2120 1148 907 677 340 245 72
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4018
Fragments arranged by size:
4018 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5509
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5509
NotI GC'GGCC_GC
Cuts at: 2956 2956
Size: 5509
NspI r_CATG'y
Cuts at: 1132 1229 2065 2155 2867 4412 1132
Size: 97 836 90 712 1545 2229
Fragments arranged by size:
2229 1545 836 712 97 90
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5509
PciI A'CATG_T
Cuts at: 4408 4408
Size: 5509
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5509
PmeI GTTT'AAAC
Cuts at: 2813 2813
Size: 5509
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2006 2038 3578 3610 5091 5123 2006
Size: 32 1540 32 1481 32 2392
Fragments arranged by size:
2392 1540 1481 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5509
PsiI TTA'TAA
Cuts at: 3079 3079
Size: 5509
PspOMI G'GGCC_C
Cuts at: 2782 2782
Size: 5509
PstI C_TGCA'G
Cuts at: 830 2091 2413 2861 830
Size: 1261 322 448 3478
Fragments arranged by size:
3478 1261 448 322
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5509
PvuII CAG'CTG
Cuts at: 2585 2585
Size: 5509
SalI G'TCGA_C
Cuts at: 1936 2052 2851 1936
Size: 116 799 4594
Fragments arranged by size:
4594 799 116
SbfI CC_TGCA'GG
Cuts at: 2861 2861
Size: 5509
ScaI AGT'ACT
Cuts at: 3757 5469 3757
Size: 1712 3797
SfcI C'TryA_G
Cuts at: 826 1045 2087 2409 2857 3992 4673 4864 826
Size: 219 1042 322 448 1135 681 191 1471
Fragments arranged by size:
1471 1135 1042 681 448 322 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5509
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5509
SmaI CCC'GGG
Cuts at: 1872 2836 1872
Size: 964 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3613 4514 4776 820
Size: 197 926 1670 901 262 1553
Fragments arranged by size:
1670 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3300 493
Size: 2807 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5509
SphI G_CATG'C
Cuts at: 1132 2867 1132
Size: 1735 3774
SspI AAT'ATT
Cuts at: 5 52 3433 5
Size: 47 3381 2081
Fragments arranged by size:
3381 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5509
StyI C'CwwG_G
Cuts at: 513 1065 2004 2948 513
Size: 552 939 944 3074
Fragments arranged by size:
3074 944 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2249 3536 3553 3706 3891 4336 325
Size: 1255 669 1287 17 153 185 445 1498
Fragments arranged by size:
1498 1287 1255 669 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2214 3755 5467
Size: 274 80 33 51 1443 237 1541 1712
Cuts at: 5467 96
Size: 138
Fragments arranged by size:
1712 1541 1443 274 237 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5469 5501 5469
Size: 32 5477
Tth111I GACn'n_nGTC
Cuts at: 1316 2397 1316
Size: 1081 4428
XbaI T'CTAG_A
Cuts at: 2845 2845
Size: 5509
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5509
XmaI C'CCGG_G
Cuts at: 1870 2834 1870
Size: 964 4545
XmnI GAAnn'nnTTC
Cuts at: 2325 2345 3638 2325
Size: 20 1293 4196
Fragments arranged by size:
4196 1293 20
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3317 276
Size: 53 83 186 936 1783 2468
Fragments arranged by size:
2468 1783 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NcoI NdeI NheI
NotI NspI PasI PciI PflMI PmeI PpiI PshAI
PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI
SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI BsiWI BstXI DraIII EcoICRI EcoRV FalI FseI
FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI
PfoI PmlI PpuMI PspXI PsrI RsrII SacI SacII
SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI