(Circular) (Six-base) MAPSORT of: pFN21AB8143.seq Check: 6375 from: 1 to: 5923 pFN21AB8143 5923 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2282 2282 Size: 5923 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3733 278 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3266 1937 Size: 1329 4594 Acc65I G'GTAC_C Cuts at: 2183 3244 2183 Size: 1061 4862 AclI AA'CG_TT Cuts at: 1563 4050 4423 1563 Size: 2487 373 3063 Fragments arranged by size: 3063 2487 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2609 3986 5370 5638 1632 Size: 977 1377 1384 268 1917 Fragments arranged by size: 1917 1384 1377 977 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5726 AflIII A'CryG_T Cuts at: 1203 2484 4822 5799 1203 Size: 1281 2338 977 1327 Fragments arranged by size: 2338 1327 1281 977 AgeI A'CCGG_T Cuts at: 4754 4754 Size: 5923 AhdI GACnn_n'nnGTC Cuts at: 4652 4652 Size: 5923 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5923 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5923 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2684 2716 2684 Size: 32 5891 AlwNI CAG_nnn'CTG Cuts at: 2073 2530 3666 5238 2073 Size: 457 1136 1572 2758 Fragments arranged by size: 2758 1572 1136 457 ApaI G_GGCC'C Cuts at: 2871 2871 Size: 5923 ApaLI G'TGCA_C Cuts at: 3980 5136 3980 Size: 1156 4767 ApoI r'AATT_y Cuts at: 1493 3232 3458 3610 5719 1493 Size: 1739 226 152 2109 1697 Fragments arranged by size: 2109 1739 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4477 160 Size: 872 3445 1606 Fragments arranged by size: 3445 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 AvaI C'yCGr_G Cuts at: 1870 1943 2870 3056 3074 3089 3248 1870 Size: 73 927 186 18 15 159 4545 Fragments arranged by size: 4545 927 186 159 73 18 15 BamHI G'GATC_C Cuts at: 3253 3620 3253 Size: 367 5556 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2183 2534 2738 2745 3244 Size: 325 217 627 396 351 204 7 499 Cuts at: 3244 4699 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 499 396 351 325 217 204 7 BanII G_rGCy'C Cuts at: 1391 2814 2871 3081 3242 1391 Size: 1423 57 210 161 4072 Fragments arranged by size: 4072 1423 210 161 57 BbeI G_GCGC'C Cuts at: 2538 2742 2749 2538 Size: 204 7 5712 Fragments arranged by size: 5712 204 7 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3112 928 Size: 971 1213 3739 Fragments arranged by size: 3739 1213 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4114 4148 1903 Size: 34 2177 34 3678 Fragments arranged by size: 3678 2177 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3815 5025 3815 Size: 1210 4713 BclI T'GATC_A Cuts at: 1548 1548 Size: 5923 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4534 136 Size: 107 122 71 631 605 2862 1525 Fragments arranged by size: 2862 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5918 5918 Size: 5923 BlpI GC'TnA_GC Cuts at: 2726 3340 2726 Size: 614 5309 Bme1580I G_kGCm'C Cuts at: 1790 2871 3984 5140 1790 Size: 1081 1113 1156 2573 Fragments arranged by size: 2573 1156 1113 1081 BmgBI CAC'GTC Cuts at: 1318 2080 1318 Size: 762 5161 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2415 3654 4612 Size: 416 53 83 399 24 998 1239 958 Cuts at: 4612 442 Size: 1753 Fragments arranged by size: 1753 1239 998 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5923 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2119 2600 2819 3005 4583 1239 Size: 880 481 219 186 1578 2579 Fragments arranged by size: 2579 1578 880 481 219 186 Bpu10I CC'TnA_GC Cuts at: 2205 2205 Size: 5923 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4048 4913 5211 4048 Size: 865 298 4760 Fragments arranged by size: 4760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2645 4586 882 Size: 617 1146 1941 2219 Fragments arranged by size: 2219 1941 1146 617 BsaAI yAC'GTr Cuts at: 493 1204 3714 493 Size: 711 2510 2702 Fragments arranged by size: 2702 2510 711 BsaBI GATnn'nnATC Cuts at: 5917 5917 Size: 5923 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2460 2535 2739 2746 Size: 53 83 186 936 927 75 204 7 Cuts at: 2746 2969 3730 4112 4747 275 Size: 223 761 382 635 1451 Fragments arranged by size: 1451 936 927 761 635 382 223 204 186 83 75 53 7 BsaWI w'CCGG_w Cuts at: 2172 2431 4356 4754 5028 5175 5586 2172 Size: 259 1925 398 274 147 411 2509 Fragments arranged by size: 2509 1925 411 398 274 259 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2684 2714 1178 Size: 30 165 30 1281 30 4387 Fragments arranged by size: 4387 1281 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5923 BseYI C'CCAG_C Cuts at: 3068 3080 3128 5126 5622 3068 Size: 12 48 1998 496 3369 Fragments arranged by size: 3369 1998 496 48 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3083 1163 Size: 573 1347 4003 Fragments arranged by size: 4003 1347 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2783 3114 3216 3373 4134 5162 Size: 275 186 782 331 102 157 761 1028 Cuts at: 5162 1540 Size: 2301 Fragments arranged by size: 2301 1028 782 761 331 275 186 157 102 BsiHKAI G_wGCw'C Cuts at: 2814 2991 3984 4069 5140 2814 Size: 177 993 85 1071 3597 Fragments arranged by size: 3597 1071 993 177 85 BsmI GAATG_Cn' Cuts at: 3434 3527 3434 Size: 93 5830 BsmBI CGTCTCn'nnnn_ Cuts at: 2576 2576 Size: 5923 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2814 2871 2991 3081 3242 3984 4069 Size: 399 1024 57 120 90 161 742 85 Cuts at: 4069 5140 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1024 742 399 161 120 90 85 57 BspEI T'CCGG_A Cuts at: 2431 5586 2431 Size: 3155 2768 BspHI T'CATG_A Cuts at: 3812 5542 3812 Size: 1730 4193 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2282 3278 844 Size: 1438 996 3489 Fragments arranged by size: 3489 1438 996 BsrBI CCG'CTC Cuts at: 1394 1823 2710 3810 1394 Size: 429 887 1100 3507 Fragments arranged by size: 3507 1100 887 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4418 4592 65 Size: 1183 3170 174 1396 Fragments arranged by size: 3170 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2018 2654 2694 4567 4754 1700 Size: 110 208 636 40 1873 187 2869 Fragments arranged by size: 2869 1873 636 208 187 110 40 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5923 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5923 BssSI C'ACGA_G Cuts at: 3983 4995 3983 Size: 1012 4911 BstAPI GCAn_nnn'nTGC Cuts at: 794 2995 794 Size: 2201 3722 BstBI TT'CG_AA Cuts at: 4727 4727 Size: 5923 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5923 BstXI CCAn_nnnn'nTGG Cuts at: 2578 2578 Size: 5923 BstYI r'GATC_y Cuts at: 1972 3253 3620 4005 4022 5474 5560 5572 5918 Size: 1281 367 385 17 1452 86 12 346 Cuts at: 5918 1972 Size: 1977 Fragments arranged by size: 1977 1452 1281 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2406 513 Size: 552 939 402 4030 Fragments arranged by size: 4030 939 552 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5184 Fragments arranged by size: 5184 683 56 BtsI GCAGTG_nn' Cuts at: 2377 2419 3439 4233 4253 2377 Size: 42 1020 794 20 4047 Fragments arranged by size: 4047 1020 794 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5923 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5888 DraI TTT'AAA Cuts at: 3227 3227 Size: 5923 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4930 809 Size: 507 3614 1802 Fragments arranged by size: 3614 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2400 2780 3111 Size: 54 1425 50 275 18 570 380 331 Cuts at: 3111 3213 3370 4259 5888 8 Size: 102 157 889 1629 43 Fragments arranged by size: 1629 1425 889 570 380 331 275 157 102 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2780 3111 3213 3370 1537 Size: 275 968 331 102 157 4090 Fragments arranged by size: 4090 968 331 275 157 102 EarI CTCTTCn'nnn_ Cuts at: 1353 3853 1353 Size: 2500 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4510 4884 5030 387 Size: 1064 138 2921 374 146 1280 Fragments arranged by size: 2921 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2812 2812 Size: 5923 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2119 2600 2609 2819 3005 3986 4583 Size: 393 487 481 9 210 186 981 597 Cuts at: 4583 5370 5638 1239 Size: 787 268 1524 Fragments arranged by size: 1524 981 787 597 487 481 393 268 210 186 9 EcoO109I rG'GnC_Cy Cuts at: 2070 2900 2070 Size: 830 5093 EcoRI G'AATT_C Cuts at: 3232 3610 3232 Size: 378 5545 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2182 2614 2182 Size: 432 5491 FspI TGC'GCA Cuts at: 2194 4429 2194 Size: 2235 3688 HaeII r_GCGC'y Cuts at: 1678 2481 2538 2701 2733 2742 2749 5070 1678 Size: 803 57 163 32 9 7 2321 2531 Fragments arranged by size: 2531 2321 803 163 57 32 9 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4564 4596 4638 4670 1373 Size: 32 135 32 2992 32 42 32 2626 Fragments arranged by size: 2992 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3157 3267 3513 669 Size: 988 281 1219 110 246 3079 Fragments arranged by size: 3079 1219 988 281 246 110 HindIII A'AGCT_T Cuts at: 748 748 Size: 5923 HpaI GTT'AAC Cuts at: 3513 3513 Size: 5923 KasI G'GCGC_C Cuts at: 2534 2738 2745 2534 Size: 204 7 5712 Fragments arranged by size: 5712 204 7 KpnI G_GTAC'C Cuts at: 2187 3248 2187 Size: 1061 4862 MfeI C'AATT_G Cuts at: 3522 3522 Size: 5923 MluI A'CGCG_T Cuts at: 5799 5799 Size: 5923 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2469 5037 5221 1305 Size: 1164 2568 184 2007 Fragments arranged by size: 2568 2007 1164 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4101 Fragments arranged by size: 4101 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 3881 4240 4399 518 Size: 648 1110 1605 359 159 2042 Fragments arranged by size: 2042 1605 1110 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2578 2641 2725 2785 2995 3028 3339 4016 Size: 972 63 84 60 210 33 311 677 Cuts at: 4016 5164 5409 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 972 677 311 245 210 84 63 60 33 NaeI GCC'GGC Cuts at: 2020 2020 Size: 5923 NarI GG'CG_CC Cuts at: 2535 2739 2746 2535 Size: 204 7 5712 Fragments arranged by size: 5712 204 7 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4432 Fragments arranged by size: 4432 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5923 NgoMIV G'CCGG_C Cuts at: 2018 2018 Size: 5923 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5923 NotI GC'GGCC_GC Cuts at: 2780 3370 2780 Size: 590 5333 NspI r_CATG'y Cuts at: 1132 1229 2668 3281 4826 1132 Size: 97 1439 613 1545 2229 Fragments arranged by size: 2229 1545 1439 613 97 PasI CC'CwG_GG Cuts at: 1348 2734 2974 1348 Size: 1386 240 4297 Fragments arranged by size: 4297 1386 240 PciI A'CATG_T Cuts at: 4822 4822 Size: 5923 PflMI CCAn_nnn'nTGG Cuts at: 1108 2530 1108 Size: 1422 4501 PfoI T'CCnGG_A Cuts at: 2037 2963 2037 Size: 926 4997 PmeI GTTT'AAAC Cuts at: 3227 3227 Size: 5923 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2321 2353 2684 2716 3992 4024 5505 5537 2321 Size: 32 331 32 1276 32 1481 32 2707 Fragments arranged by size: 2707 1481 1276 331 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5923 PsiI TTA'TAA Cuts at: 3493 3493 Size: 5923 PspOMI G'GGCC_C Cuts at: 2867 2867 Size: 5923 PstI C_TGCA'G Cuts at: 830 3275 830 Size: 2445 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5923 PvuII CAG'CTG Cuts at: 2578 2641 2725 2578 Size: 63 84 5776 Fragments arranged by size: 5776 84 63 SacI G_AGCT'C Cuts at: 2814 2814 Size: 5923 SalI G'TCGA_C Cuts at: 1936 3265 1936 Size: 1329 4594 SbfI CC_TGCA'GG Cuts at: 3275 3275 Size: 5923 ScaI AGT'ACT Cuts at: 2861 4171 5883 2861 Size: 1310 1712 2901 Fragments arranged by size: 2901 1712 1310 SexAI A'CCwGG_T Cuts at: 2226 2226 Size: 5923 SfcI C'TryA_G Cuts at: 826 1045 3271 4406 5087 5278 826 Size: 219 2226 1135 681 191 1471 Fragments arranged by size: 2226 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2536 2740 2747 2536 Size: 204 7 5712 Fragments arranged by size: 5712 204 7 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5923 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5923 SmaI CCC'GGG Cuts at: 1872 2872 3058 3091 3250 1872 Size: 1000 186 33 159 4545 Fragments arranged by size: 4545 1000 186 159 33 SmlI C'TyrA_G Cuts at: 820 1017 1943 4027 4928 5190 820 Size: 197 926 2084 901 262 1553 Fragments arranged by size: 2084 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3714 493 Size: 3221 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5923 SphI G_CATG'C Cuts at: 1132 3281 1132 Size: 2149 3774 SrfI GCCC'GGGC Cuts at: 3091 3091 Size: 5923 SspI AAT'ATT Cuts at: 5 52 3847 5 Size: 47 3795 2081 Fragments arranged by size: 3795 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5923 StyI C'CwwG_G Cuts at: 513 1065 2004 2463 2703 3362 513 Size: 552 939 459 240 659 3074 Fragments arranged by size: 3074 939 659 552 459 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2135 2626 2966 3066 3142 3950 3967 Size: 1255 555 491 340 100 76 808 17 Cuts at: 3967 4120 4305 4750 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 808 555 491 445 340 185 153 100 76 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2859 4169 5881 Size: 274 80 33 51 1443 882 1310 1712 Cuts at: 5881 96 Size: 138 Fragments arranged by size: 1712 1443 1310 882 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5883 5915 5883 Size: 32 5891 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5923 XbaI T'CTAG_A Cuts at: 3259 3259 Size: 5923 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5923 XmaI C'CCGG_G Cuts at: 1870 2870 3056 3089 3248 1870 Size: 1000 186 33 159 4545 Fragments arranged by size: 4545 1000 186 159 33 XmnI GAAnn'nnTTC Cuts at: 2129 2165 2267 2336 4052 2129 Size: 36 102 69 1716 4000 Fragments arranged by size: 4000 1716 102 69 36 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3731 276 Size: 53 83 186 936 2197 2468 Fragments arranged by size: 2468 2197 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SwaI XcmI