Restriction map for
pFN21AB8137
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EarI
EclXI Eco52I Eco81I EcoNI EcoRI
EcoRV FbaI FseI FspI HapII
HincII HindII HindIII HpaI HpaII
KasI Ksp632I KspI MaeII MamI
MfeI MflI MluI MluNI MroI
MscI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 801 GGTACC G^GTACC
AccII 948 CGCG CG^CG
AluI 774 AGCT AG^CT
AlwNI 556 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 727 AGCGCT AGC^GCT
Asp718I 801 GGTACC G^GTACC
BanI 801 GGTACC G^G[CT][GA]CC
BsaI 997 GAGACC GGTCTC(1/5)
BseRI 925 GAGGAG GAGGAG(10/8)
Bsp1407I 412 TGTACA T^GTACA
BspHI 455 TCATGA T^CATGA
BspMI 613 ACCTGC ACCTGC(4/8)
BsrGI 412 TGTACA T^GTACA
BssKI 1023 CCAGG ^CC[ACGT]GG
BstNI 1023 CCAGG CC^[AT]GG
BstUI 948 CGCG CG^CG
Cac8I 651 GCCAGC GC[ACGT]^[ACGT]GC
DraII 244 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 905 CGGCCA [CT]^GGCC[GA]
Eco47III 727 AGCGCT AGC^GCT
EcoO109I 244 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRII 1023 CCAGG ^CC[AT]GG
EcoT22I 526 ATGCAT ATGCA^T
HaeII 727 AGCGCT [GA]GCGC^[CT]
KpnI 801 GGTACC GGTAC^C
MseI 193 TTAA T^TAA
MspA1I 773 CAGCTG C[AC]G^C[GT]G
MvaI 1023 CCAGG CC^[AT]GG
MvnI 948 CGCG CG^CG
NcoI 371 CCATGG C^CATGG
NsiI 526 ATGCAT ATGCA^T
PpuMI 244 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 113 GACTGCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 115 CTGCAG CTGCA^G
PvuII 773 CAGCTG CAG^CTG
RcaI 455 TCATGA T^CATGA
ScrFI 1023 CCAGG CC^[ACGT]GG
SfcI 115 CTGCAG C^T[GA][CT]AG
SmlI 992 CTCAAG C^T[CT][GA]AG
SspBI 412 TGTACA T^GTACA
SspI 540 AATATT AAT^ATT
Tru9I 193 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 169 GAATTT [GA]^AATT[CT]
699 AAATTC
AcyI 154 GACGCC G[GA]^CG[CT]C
184 GACGCC
ApoI 169 GAATTT [GA]^AATT[CT]
699 AAATTC
AvaI 507 CCCGAG C^[CT]CG[GA]G
596 CCCGAG
AvaII 220 GGACC G^G[AT]CC
245 GGACC
BbsI 609 GAAGAC GAAGAC(2/6)
765 GAAGAC
BfaI 520 CTAG C^TAG
878 CTAG
BpuAI 609 GAAGAC GAAGAC(2/6)
765 GAAGAC
BsaHI 154 GACGCC G[GA]^CG[CT]C
184 GACGCC
BsoBI 507 CCCGAG C^[CT]CG[GA]G
596 CCCGAG
BstEII 288 GGTGACC G^GT[ACGT]ACC
375 GGTCACC
BstPI 288 GGTGACC G^GT[ACGT]ACC
375 GGTCACC
BstXI 344 CCATCACGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
908 CCAACGGCATGG
CfoI 728 GCGC GCG^C
949 GCGC
DdeI 589 CTCAG C^T[ACGT]AG
784 CTGAG
DpnII 429 GATC ^GATC
451 GATC
DsaI 371 CCATGG C^C[GA][CT]GG
967 CCGTGG
EcoO65I 288 GGTGACC G^GT[ACGT]ACC
375 GGTCACC
EcoT14I 371 CCATGG C^C[AT][AT]GG
404 CCAAGG
FauI 946 CCCGC CCCGC(4/6)
985 CCCGC
HaeIII 43 GGCC GG^CC
906 GGCC
HgaI 154 GACGC GACGC(5/10)
184 GACGC
HhaI 728 GCGC GCG^C
949 GCGC
Hin1I 154 GACGCC G[GA]^CG[CT]C
184 GACGCC
HinP1I 728 GCGC G^CGC
949 GCGC
MaeI 520 CTAG C^TAG
878 CTAG
MboI 429 GATC ^GATC
451 GATC
MslI 345 CATCACGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
529 CATTTGTGTG
NdeII 429 GATC ^GATC
451 GATC
PleI 325 GAGTC GAGTC(4/5)
786 GAGTC
Sau3AI 429 GATC ^GATC
451 GATC
SfaNI 901 GATGC GCATC(5/9)
515 GCATC
StyI 371 CCATGG C^C[AT][AT]GG
404 CCAAGG
TaqI 319 TCGA T^CGA
568 TCGA
TfiI 92 GAATC G^A[AT]TC
461 GAATC
TthHB8I 319 TCGA T^CGA
568 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AciI AfaI BbvI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BstF5I
Cfr13I Fnu4HI FokI HinfI HphI
ItaI MaeIII MboII MnlI MwoI
NlaIII NlaIV RsaI Sau96I TseI
Tsp45I Tsp509I TspRI