Restriction map for
pFN21AB8136
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# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BanI BbeI BcgI
BciVI BclI BfrI BglI BglII
BlnI BsaBI BsaHI BsaWI BseAI
BsiEI BsmI Bsp1407I BspDI BspEI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstYI
BstZ17I Cfr10I ClaI CpoI DraI
EagI Eam1105I EclXI Eco52I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MflI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SexAI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI SspBI
SspI StuI SwaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 46 enzymes) #
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AlwI 446 GGATC GGATC(4/5)
AocI 963 CCTGAGG CC^T[ACGT]AGG
Aor51HI 820 AGCGCT AGC^GCT
ApaI 1084 GGGCCC GGGCC^C
Asp700I 163 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 107 TGGCCA TGG^CCA
BanII 1084 GGGCCC G[GA]GC[CT]^C
BbvCI 816 CCTCAGC CCTCAGC(-5/-2)
BlpI 1074 GCTCAGC GC^T[ACGT]AGC
BpmI 1293 CTGGAG CTGGAG(16/14)
Bpu1102I 1074 GCTCAGC GC^T[ACGT]AGC
BsiWI 803 CGTACG C^GTACG
BsmBI 1157 GAGACG CGTCTC(1/5)
BspLU11I 1211 ACATGT A^CATGT
BsrBI 485 GAGCGG CCGCTC(-3/-3)
BstXI 506 CCAGTGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 963 CCTGAGG CC^T[ACGT]AGG
CelII 1074 GCTCAGC GC^T[ACGT]AGC
DraIII 742 CACCTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1132 GACGACCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 728 CCATGG C^C[GA][CT]GG
EaeI 107 TGGCCA [CT]^GGCC[GA]
Eco47III 820 AGCGCT AGC^GCT
Eco81I 963 CCTGAGG CC^T[ACGT]AGG
EcoT14I 728 CCATGG C^C[AT][AT]GG
HaeII 820 AGCGCT [GA]GCGC^[CT]
MluI 800 ACGCGT A^CGCGT
MluNI 107 TGGCCA TGG^CCA
MscI 107 TGGCCA TGG^CCA
MseI 842 TTAA T^TAA
MspA1I 532 CAGCTG C[AC]G^C[GT]G
NcoI 728 CCATGG C^CATGG
NspI 1211 ACATGT [GA]CATG^[CT]
PflMI 1100 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 670 GAGTC GAGTC(4/5)
PvuII 532 CAGCTG CAG^CTG
ScaI 302 AGTACT AGT^ACT
SfaNI 691 GATGC GCATC(5/9)
SmaI 686 CCCGGG CCC^GGG
StyI 728 CCATGG C^C[AT][AT]GG
Tru9I 842 TTAA T^TAA
Van91I 1100 CCACACACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 954 TCTAGA T^CTAGA
XmaCI 686 CCCGGG C^CCGGG
XmaI 686 CCCGGG C^CCGGG
XmnI 163 GAAAAAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 167 AAATTC [GA]^AATT[CT]
944 AAATTT
Alw44I 464 GTGCAC G^TGCAC
909 GTGCAC
ApaLI 464 GTGCAC G^TGCAC
909 GTGCAC
ApoI 167 AAATTC [GA]^AATT[CT]
944 AAATTT
BbrPI 259 CACGTG CAC^GTG
285 CACGTG
BbsI 889 GAAGAC GAAGAC(2/6)
1282 GTCTTC
BfaI 482 CTAG C^TAG
955 CTAG
BpuAI 889 GAAGAC GAAGAC(2/6)
1282 GTCTTC
BsaAI 259 CACGTG [CT]AC^GT[GA]
285 CACGTG
BseRI 97 GAGGAG GAGGAG(10/8)
158 GAGGAG
BsgI 1006 CTGCAC GTGCAG(16/14)
57 GTGCAG
BspHI 662 TCATGA T^CATGA
1124 TCATGA
BspMI 643 ACCTGC ACCTGC(4/8)
1068 GCAGGT
BstAPI 466 GCACACTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
741 GCACCTTGTGC
EcoNI 75 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1047 CCTGCAGGAGG
HgaI 1116 GACGC GACGC(5/10)
1031 GCGTC
MaeI 482 CTAG C^TAG
955 CTAG
PmaCI 259 CACGTG CAC^GTG
285 CACGTG
PmlI 259 CACGTG CAC^GTG
285 CACGTG
PpuMI 640 GGGACCT [GA]G^G[AT]CC[CT]
1000 GGGACCC
RcaI 662 TCATGA T^CATGA
1124 TCATGA
SapI 43 GAAGAGC GCTCTTC(1/4)
786 GCTCTTC
SbfI 75 CCTGCAGG CCTGCA^GG
1047 CCTGCAGG
Sse8387I 75 CCTGCAGG CCTGCA^GG
1047 CCTGCAGG
TaqI 668 TCGA T^CGA
983 TCGA
TfiI 31 GAATC G^A[AT]TC
400 GAATC
Tsp509I 168 AATT ^AATT
945 AATT
TthHB8I 668 TCGA T^CGA
983 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AspI AvaI AvaII
BbvI BcnI BmyI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PflFI PstI RsaI
Sau3AI Sau96I ScrFI SfcI TseI
Tsp45I TspRI Tth111I