(Circular) (Six-base) MAPSORT of: pFN21AB8136.seq Check: 4454 from: 1 to: 6004 pFN21AB8136 6004 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3064 3064 Size: 6004 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3814 278 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3347 1937 Size: 1410 4594 Acc65I G'GTAC_C Cuts at: 3325 3325 Size: 6004 AclI AA'CG_TT Cuts at: 1563 4131 4504 1563 Size: 2568 373 3063 Fragments arranged by size: 3063 2568 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2726 4067 5451 5719 1632 Size: 1094 1341 1384 268 1917 Fragments arranged by size: 1917 1384 1341 1094 268 AfeI AGC'GCT Cuts at: 2827 2827 Size: 6004 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5807 AflIII A'CryG_T Cuts at: 1203 2289 2805 3216 4903 5880 1203 Size: 1086 516 411 1687 977 1327 Fragments arranged by size: 1687 1327 1086 977 516 411 AgeI A'CCGG_T Cuts at: 4835 4835 Size: 6004 AhdI GACnn_n'nnGTC Cuts at: 4733 4733 Size: 6004 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6004 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2939 1184 Size: 1755 4249 AlwNI CAG_nnn'CTG Cuts at: 3052 3124 3149 3747 5319 3052 Size: 72 25 598 1572 3737 Fragments arranged by size: 3737 1572 598 72 25 ApaI G_GGCC'C Cuts at: 3093 3093 Size: 6004 ApaLI G'TGCA_C Cuts at: 2469 2914 4061 5217 2469 Size: 445 1147 1156 3256 Fragments arranged by size: 3256 1156 1147 445 ApoI r'AATT_y Cuts at: 1493 2172 2949 3313 3539 3691 5800 1493 Size: 679 777 364 226 152 2109 1697 Fragments arranged by size: 2109 1697 777 679 364 226 152 AseI AT'TA_AT Cuts at: 160 1032 4558 160 Size: 872 3526 1606 Fragments arranged by size: 3526 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 AvaI C'yCGr_G Cuts at: 1870 1943 2282 2609 2691 3329 1870 Size: 73 339 327 82 638 4545 Fragments arranged by size: 4545 638 339 327 82 73 BamHI G'GATC_C Cuts at: 3334 3701 3334 Size: 367 5637 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3325 4780 618 Size: 325 217 627 1538 1455 1842 Fragments arranged by size: 1842 1538 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3093 3323 1391 Size: 1702 230 4072 Fragments arranged by size: 4072 1702 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2901 3280 928 Size: 971 1002 379 3652 Fragments arranged by size: 3652 1002 971 379 BbvCI CC'TCA_GC Cuts at: 2822 2822 Size: 6004 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4195 4229 1903 Size: 34 2258 34 3678 Fragments arranged by size: 3678 2258 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3896 5106 3896 Size: 1210 4794 BclI T'GATC_A Cuts at: 1548 1548 Size: 6004 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4615 136 Size: 107 122 71 631 605 2943 1525 Fragments arranged by size: 2943 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5999 5999 Size: 6004 BlpI GC'TnA_GC Cuts at: 3080 3421 3080 Size: 341 5663 Bme1580I G_kGCm'C Cuts at: 1790 2473 2757 2918 3093 4065 5221 1790 Size: 683 284 161 175 972 1156 2573 Fragments arranged by size: 2573 1156 972 683 284 175 161 BmgBI CAC'GTC Cuts at: 1318 2434 2623 3166 1318 Size: 1116 189 543 4156 Fragments arranged by size: 4156 1116 543 189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2457 3137 3194 Size: 416 53 83 399 24 1040 680 57 Cuts at: 3194 3735 4693 442 Size: 541 958 1753 Fragments arranged by size: 1753 1040 958 680 541 416 399 83 57 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6004 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3319 4664 1239 Size: 2080 1345 2579 Fragments arranged by size: 2579 2080 1345 Bpu10I CC'TnA_GC Cuts at: 2822 2822 Size: 6004 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4129 4994 5292 4129 Size: 865 298 4841 Fragments arranged by size: 4841 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2081 2135 2957 4667 882 Size: 617 582 54 822 1710 2219 Fragments arranged by size: 2219 1710 822 617 582 54 BsaAI yAC'GTr Cuts at: 493 1204 2266 2292 3795 493 Size: 711 1062 26 1503 2702 Fragments arranged by size: 2702 1503 1062 711 26 BsaBI GATnn'nnATC Cuts at: 5998 5998 Size: 6004 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3811 4193 4828 275 Size: 53 83 186 936 2278 382 635 1451 Fragments arranged by size: 2278 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4437 4835 5109 5256 5667 4437 Size: 398 274 147 411 4774 Fragments arranged by size: 4774 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3153 3183 3270 3300 1178 Size: 30 165 30 1750 30 87 30 3882 Fragments arranged by size: 3882 1750 165 87 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2117 2178 1186 Size: 931 61 5012 Fragments arranged by size: 5012 931 61 BseYI C'CCAG_C Cuts at: 2145 3234 5207 5703 2145 Size: 1089 1973 496 2446 Fragments arranged by size: 2446 1973 1089 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2083 2996 1163 Size: 573 347 913 4171 Fragments arranged by size: 4171 913 573 347 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3454 4215 5243 1540 Size: 275 186 1453 761 1028 2301 Fragments arranged by size: 2301 1453 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2473 2793 2918 3081 4065 4150 5221 2473 Size: 320 125 163 984 85 1071 3256 Fragments arranged by size: 3256 1071 984 320 163 125 85 BsiWI C'GTAC_G Cuts at: 2808 2808 Size: 6004 BsmI GAATG_Cn' Cuts at: 3515 3608 3515 Size: 93 5911 BsmBI CGTCTCn'nnnn_ Cuts at: 3156 3156 Size: 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2473 2757 2793 2918 3081 3093 3323 Size: 399 683 284 36 125 163 12 230 Cuts at: 3323 4065 4150 5221 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 683 399 284 230 163 125 85 36 12 BspEI T'CCGG_A Cuts at: 5667 5667 Size: 6004 BspHI T'CATG_A Cuts at: 2667 3129 3893 5623 2667 Size: 462 764 1730 3048 Fragments arranged by size: 3048 1730 764 462 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2657 3064 3359 844 Size: 1813 407 295 3489 Fragments arranged by size: 3489 1813 407 295 BsrBI CCG'CTC Cuts at: 1394 1823 2492 3891 1394 Size: 429 669 1399 3507 Fragments arranged by size: 3507 1399 669 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4499 4673 65 Size: 1183 3251 174 1396 Fragments arranged by size: 3251 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4648 4835 1700 Size: 110 2838 187 2869 Fragments arranged by size: 2869 2838 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6004 BssSI C'ACGA_G Cuts at: 4064 5076 4064 Size: 1012 4992 BstAPI GCAn_nnn'nTGC Cuts at: 794 2477 2752 794 Size: 1683 275 4046 Fragments arranged by size: 4046 1683 275 BstBI TT'CG_AA Cuts at: 4808 4808 Size: 6004 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6004 BstXI CCAn_nnnn'nTGG Cuts at: 2518 2518 Size: 6004 BstYI r'GATC_y Cuts at: 1972 3334 3701 4086 4103 5555 5641 5653 5999 Size: 1362 367 385 17 1452 86 12 346 Cuts at: 5999 1972 Size: 1977 Fragments arranged by size: 1977 1452 1362 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2969 2969 Size: 6004 BtgI C'CryG_G Cuts at: 513 1065 2004 2733 513 Size: 552 939 729 3784 Fragments arranged by size: 3784 939 729 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2101 487 Size: 683 56 875 4390 Fragments arranged by size: 4390 875 683 56 BtsI GCAGTG_nn' Cuts at: 2071 2193 2767 3520 4314 4334 2071 Size: 122 574 753 794 20 3741 Fragments arranged by size: 3741 794 753 574 122 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6004 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2235 2270 529 Size: 35 1671 35 4263 Fragments arranged by size: 4263 1671 35 35 DraI TTT'AAA Cuts at: 3308 3308 Size: 6004 DraIII CAC_nnn'GTG Cuts at: 2752 2752 Size: 6004 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3143 5011 809 Size: 507 1827 1868 1802 Fragments arranged by size: 1868 1827 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 3451 4340 Size: 54 1425 50 275 18 282 1339 889 Cuts at: 4340 5969 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1339 889 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3451 1537 Size: 275 1639 4090 Fragments arranged by size: 4090 1639 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2043 2298 2468 2798 3934 1353 Size: 690 255 170 330 1136 3423 Fragments arranged by size: 3423 1136 690 330 255 170 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4591 4965 5111 387 Size: 1064 138 3002 374 146 1280 Fragments arranged by size: 3002 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2726 3319 4067 4664 5451 5719 1239 Size: 393 1094 593 748 597 787 268 1524 Fragments arranged by size: 1524 1094 787 748 597 593 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2084 3056 2084 Size: 972 5032 EcoO109I rG'GnC_Cy Cuts at: 2646 3006 3090 2646 Size: 360 84 5560 Fragments arranged by size: 5560 360 84 EcoRI G'AATT_C Cuts at: 3313 3691 3313 Size: 378 5626 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2809 2809 Size: 6004 FspI TGC'GCA Cuts at: 4510 4510 Size: 6004 HaeII r_GCGC'y Cuts at: 1678 2829 5151 1678 Size: 1151 2322 2531 Fragments arranged by size: 2531 2322 1151 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2475 2888 2920 3153 Size: 32 135 32 871 32 413 32 233 Cuts at: 3153 3185 3248 3270 3280 3302 4645 4677 4719 Size: 32 63 22 10 22 1343 32 42 Cuts at: 4719 4751 1373 Size: 32 2626 Fragments arranged by size: 2626 1343 871 413 233 135 63 42 32 32 32 32 32 32 32 22 22 10 HincII GTy'rAC Cuts at: 669 1657 1938 3348 3594 669 Size: 988 281 1410 246 3079 Fragments arranged by size: 3079 1410 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6004 HpaI GTT'AAC Cuts at: 3594 3594 Size: 6004 KpnI G_GTAC'C Cuts at: 3329 3329 Size: 6004 MfeI C'AATT_G Cuts at: 3603 3603 Size: 6004 MluI A'CGCG_T Cuts at: 2805 5880 2805 Size: 3075 2929 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2910 3256 5118 5302 1305 Size: 1605 346 1862 184 2007 Fragments arranged by size: 2007 1862 1605 346 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2114 10 Size: 54 1425 343 282 3900 Fragments arranged by size: 3900 1425 343 282 54 MslI CAynn'nnrTG Cuts at: 518 1166 2268 2573 3221 3962 4321 4480 518 Size: 648 1102 305 648 741 359 159 2042 Fragments arranged by size: 2042 1102 741 648 648 359 305 159 MspA1I CmG'CkG Cuts at: 1606 2539 3420 4097 5245 5490 1606 Size: 933 881 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 881 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2733 513 Size: 552 939 729 3784 Fragments arranged by size: 3784 939 729 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6004 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6004 NotI GC'GGCC_GC Cuts at: 3451 3451 Size: 6004 NspI r_CATG'y Cuts at: 1132 1229 3220 3362 4907 1132 Size: 97 1991 142 1545 2229 Fragments arranged by size: 2229 1991 1545 142 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6004 PciI A'CATG_T Cuts at: 3216 4903 3216 Size: 1687 4317 PflMI CCAn_nnn'nTGG Cuts at: 1108 3111 1108 Size: 2003 4001 PfoI T'CCnGG_A Cuts at: 2427 2427 Size: 6004 PmeI GTTT'AAAC Cuts at: 3308 3308 Size: 6004 PmlI CAC'GTG Cuts at: 2266 2292 2266 Size: 26 5978 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4073 4105 5586 5618 4073 Size: 32 1481 32 4459 Fragments arranged by size: 4459 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2646 3006 2646 Size: 360 5644 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6004 PsiI TTA'TAA Cuts at: 3574 3574 Size: 6004 PspOMI G'GGCC_C Cuts at: 3089 3089 Size: 6004 PstI C_TGCA'G Cuts at: 830 2085 2200 2397 2550 2762 3057 3356 830 Size: 1255 115 197 153 212 295 299 3478 Fragments arranged by size: 3478 1255 299 295 212 197 153 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6004 PvuII CAG'CTG Cuts at: 2539 2539 Size: 6004 SalI G'TCGA_C Cuts at: 1936 3346 1936 Size: 1410 4594 SanDI GG'GwC_CC Cuts at: 3006 3006 Size: 6004 SapI GCTCTTCn'nnn_ Cuts at: 2043 2798 2043 Size: 755 5249 SbfI CC_TGCA'GG Cuts at: 2085 3057 3356 2085 Size: 972 299 4733 Fragments arranged by size: 4733 972 299 ScaI AGT'ACT Cuts at: 2309 4252 5964 2309 Size: 1943 1712 2349 Fragments arranged by size: 2349 1943 1712 SfcI C'TryA_G Cuts at: 826 1045 2081 2196 2393 2546 2758 3053 3352 Size: 219 1036 115 197 153 212 295 299 Cuts at: 3352 4487 5168 5359 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 1036 681 299 295 219 212 197 191 153 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6004 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6004 SmaI CCC'GGG Cuts at: 1872 2693 3331 1872 Size: 821 638 4545 Fragments arranged by size: 4545 821 638 SmlI C'TyrA_G Cuts at: 820 1017 1943 4108 5009 5271 820 Size: 197 926 2165 901 262 1553 Fragments arranged by size: 2165 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3795 493 Size: 3302 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6004 SphI G_CATG'C Cuts at: 1132 3362 1132 Size: 2230 3774 SspI AAT'ATT Cuts at: 5 52 3928 5 Size: 47 3876 2081 Fragments arranged by size: 3876 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6004 StyI C'CwwG_G Cuts at: 513 1065 2004 2733 3443 513 Size: 552 939 729 710 3074 Fragments arranged by size: 3074 939 729 710 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4031 4048 4201 4386 4831 325 Size: 1255 2451 17 153 185 445 1498 Fragments arranged by size: 2451 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2092 2307 4250 Size: 274 80 33 51 1443 115 215 1943 Cuts at: 4250 5962 96 Size: 1712 138 Fragments arranged by size: 1943 1712 1443 274 215 138 115 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5964 5996 5964 Size: 32 5972 Tth111I GACn'n_nGTC Cuts at: 1316 2717 3143 3167 1316 Size: 1401 426 24 4153 Fragments arranged by size: 4153 1401 426 24 XbaI T'CTAG_A Cuts at: 2959 3340 2959 Size: 381 5623 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6004 XmaI C'CCGG_G Cuts at: 1870 2691 3329 1870 Size: 821 638 4545 Fragments arranged by size: 4545 821 638 XmnI GAAnn'nnTTC Cuts at: 2172 4133 2172 Size: 1961 4043 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3812 276 Size: 53 83 186 936 2278 2468 Fragments arranged by size: 2468 2278 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI XcmI