Restriction map for
pFN21AB8136
(Linear) (Six-base) MAPSORT of: KIBB8136.seq Check: 1693 from: 1 to: 1299
KIBB8136.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1059 1299
Size: 1059 240
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1299
Size: 721 578
AfeI AGC'GCT
Cuts at: 0 822 1299
Size: 822 477
AflIII A'CryG_T
Cuts at: 0 284 800 1211 1299
Size: 284 516 411 88
Fragments arranged by size:
516 411 284 88
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 934 1299
Size: 934 365
AlwNI CAG_nnn'CTG
Cuts at: 0 1047 1119 1144 1299
Size: 1047 72 25 155
Fragments arranged by size:
1047 155 72 25
ApaI G_GGCC'C
Cuts at: 0 1088 1299
Size: 1088 211
ApaLI G'TGCA_C
Cuts at: 0 464 909 1299
Size: 464 445 390
Fragments arranged by size:
464 445 390
ApoI r'AATT_y
Cuts at: 0 167 944 1299
Size: 167 777 355
Fragments arranged by size:
777 355 167
AvaI C'yCGr_G
Cuts at: 0 277 604 686 1299
Size: 277 327 82 613
Fragments arranged by size:
613 327 277 82
BanII G_rGCy'C
Cuts at: 0 1088 1299
Size: 1088 211
BbsI GAAGACnn'nnnn_
Cuts at: 0 896 1275 1299
Size: 896 379 24
Fragments arranged by size:
896 379 24
BbvCI CC'TCA_GC
Cuts at: 0 817 1299
Size: 817 482
BlpI GC'TnA_GC
Cuts at: 0 1075 1299
Size: 1075 224
Bme1580I G_kGCm'C
Cuts at: 0 468 752 913 1088 1299
Size: 468 284 161 175 211
Fragments arranged by size:
468 284 211 175 161
BmgBI CAC'GTC
Cuts at: 0 429 618 1161 1299
Size: 429 189 543 138
Fragments arranged by size:
543 429 189 138
BmrI ACTGGGnnnn_n'
Cuts at: 0 452 1132 1189 1299
Size: 452 680 57 110
Fragments arranged by size:
680 452 110 57
Bpu10I CC'TnA_GC
Cuts at: 0 817 1299
Size: 817 482
BsaI GGTCTCn'nnnn_
Cuts at: 0 76 130 952 1299
Size: 76 54 822 347
Fragments arranged by size:
822 347 76 54
BsaAI yAC'GTr
Cuts at: 0 261 287 1299
Size: 261 26 1012
Fragments arranged by size:
1012 261 26
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1148 1178 1265 1295 1299
Size: 1148 30 87 30 4
Fragments arranged by size:
1148 87 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 112 173 1299
Size: 112 61 1126
Fragments arranged by size:
1126 112 61
BseYI C'CCAG_C
Cuts at: 0 140 1229 1299
Size: 140 1089 70
Fragments arranged by size:
1089 140 70
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 991 1299
Size: 78 913 308
Fragments arranged by size:
913 308 78
BsiHKAI G_wGCw'C
Cuts at: 0 468 788 913 1076 1299
Size: 468 320 125 163 223
Fragments arranged by size:
468 320 223 163 125
BsiWI C'GTAC_G
Cuts at: 0 803 1299
Size: 803 496
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1151 1299
Size: 1151 148
Bsp1286I G_dGCh'C
Cuts at: 0 468 752 788 913 1076 1088 1299
Size: 468 284 36 125 163 12 211
Fragments arranged by size:
468 284 211 163 125 36 12
BspHI T'CATG_A
Cuts at: 0 662 1124 1299
Size: 662 462 175
Fragments arranged by size:
662 462 175
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 652 1059 1299
Size: 652 407 240
Fragments arranged by size:
652 407 240
BsrBI CCG'CTC
Cuts at: 0 487 1299
Size: 487 812
BstAPI GCAn_nnn'nTGC
Cuts at: 0 472 747 1299
Size: 472 275 552
Fragments arranged by size:
552 472 275
BstXI CCAn_nnnn'nTGG
Cuts at: 0 513 1299
Size: 513 786
Bsu36I CC'TnA_GG
Cuts at: 0 964 1299
Size: 964 335
BtgI C'CryG_G
Cuts at: 0 728 1299
Size: 728 571
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 96 1299
Size: 96 1203
BtsI GCAGTG_nn'
Cuts at: 0 66 188 762 1299
Size: 66 122 574 537
Fragments arranged by size:
574 537 122 66
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 230 265 1299
Size: 230 35 1034
Fragments arranged by size:
1034 230 35
DraIII CAC_nnn'GTG
Cuts at: 0 747 1299
Size: 747 552
DrdI GACnn_nn'nnGTC
Cuts at: 0 1138 1299
Size: 1138 161
EaeI y'GGCC_r
Cuts at: 0 107 1299
Size: 107 1192
EarI CTCTTCn'nnn_
Cuts at: 0 38 293 463 793 1299
Size: 38 255 170 330 506
Fragments arranged by size:
506 330 255 170 38
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 721 1299
Size: 721 578
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 1051 1299
Size: 79 972 248
Fragments arranged by size:
972 248 79
EcoO109I rG'GnC_Cy
Cuts at: 0 641 1001 1085 1299
Size: 641 360 84 214
Fragments arranged by size:
641 360 214 84
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 804 1299
Size: 804 495
HaeII r_GCGC'y
Cuts at: 0 824 1299
Size: 824 475
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 470 883 915 1148 1180 1243 1265
Size: 438 32 413 32 233 32 63 22
Cuts at: 1265 1275 1297 1299
Size: 10 22 2
Fragments arranged by size:
438 413 233 63 32 32 32 22
22 10 2
MluI A'CGCG_T
Cuts at: 0 800 1299
Size: 800 499
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 905 1251 1299
Size: 905 346 48
Fragments arranged by size:
905 346 48
MscI TGG'CCA
Cuts at: 0 109 1299
Size: 109 1190
MslI CAynn'nnrTG
Cuts at: 0 263 568 1216 1299
Size: 263 305 648 83
Fragments arranged by size:
648 305 263 83
MspA1I CmG'CkG
Cuts at: 0 534 1299
Size: 534 765
NcoI C'CATG_G
Cuts at: 0 728 1299
Size: 728 571
NspI r_CATG'y
Cuts at: 0 1215 1299
Size: 1215 84
PciI A'CATG_T
Cuts at: 0 1211 1299
Size: 1211 88
PflMI CCAn_nnn'nTGG
Cuts at: 0 1106 1299
Size: 1106 193
PfoI T'CCnGG_A
Cuts at: 0 422 1299
Size: 422 877
PmlI CAC'GTG
Cuts at: 0 261 287 1299
Size: 261 26 1012
Fragments arranged by size:
1012 261 26
PpuMI rG'GwC_Cy
Cuts at: 0 641 1001 1299
Size: 641 360 298
Fragments arranged by size:
641 360 298
PspOMI G'GGCC_C
Cuts at: 0 1084 1299
Size: 1084 215
PstI C_TGCA'G
Cuts at: 0 80 195 392 545 757 1052 1299
Size: 80 115 197 153 212 295 247
Fragments arranged by size:
295 247 212 197 153 115 80
PvuII CAG'CTG
Cuts at: 0 534 1299
Size: 534 765
SanDI GG'GwC_CC
Cuts at: 0 1001 1299
Size: 1001 298
SapI GCTCTTCn'nnn_
Cuts at: 0 38 793 1299
Size: 38 755 506
Fragments arranged by size:
755 506 38
SbfI CC_TGCA'GG
Cuts at: 0 80 1052 1299
Size: 80 972 247
Fragments arranged by size:
972 247 80
ScaI AGT'ACT
Cuts at: 0 304 1299
Size: 304 995
SfcI C'TryA_G
Cuts at: 0 76 191 388 541 753 1048 1299
Size: 76 115 197 153 212 295 251
Fragments arranged by size:
295 251 212 197 153 115 76
SmaI CCC'GGG
Cuts at: 0 688 1299
Size: 688 611
StyI C'CwwG_G
Cuts at: 0 728 1299
Size: 728 571
TatI w'GTAC_w
Cuts at: 0 87 302 1299
Size: 87 215 997
Fragments arranged by size:
997 215 87
Tth111I GACn'n_nGTC
Cuts at: 0 712 1138 1162 1299
Size: 712 426 24 137
Fragments arranged by size:
712 426 137 24
XbaI T'CTAG_A
Cuts at: 0 954 1299
Size: 954 345
XmaI C'CCGG_G
Cuts at: 0 686 1299
Size: 686 613
XmnI GAAnn'nnTTC
Cuts at: 0 167 1299
Size: 167 1132
Enzymes that do cut:
AarI AcuI AfeI AflIII AlfI AlwNI ApaI ApaLI
ApoI AvaI BanII BbsI BbvCI BlpI Bme1580I BmgBI
BmrI Bpu10I BsaI BsaAI BsaXI BseRI BseYI BsgI
BsiHKAI BsiWI BsmBI Bsp1286I BspHI BspMI BsrBI BstAPI
BstXI Bsu36I BtgI BtgZI BtsI CspCI DraIII DrdI
EaeI EarI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I
MluI MmeI MscI MslI MspA1I NcoI NspI PciI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SanDI
SapI SbfI ScaI SfcI SmaI StyI TatI Tth111I
XbaI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AgeI AhdI AleI
AloI AscI AseI AsiSI AvrII BaeI BamHI BanI
BbeI BcgI BciVI BclI BfrBI BglI BglII BmtI
BplI BpmI BpuEI BsaBI BsaHI BsaWI BsiEI BsmI
BspEI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstYI BstZ17I ClaI DraI EagI EciI EcoICRI EcoRI
EcoRV FseI FspI FspAI HincII HindIII HpaI KasI
KpnI MfeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI PacI PasI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SexAI
SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI XcmI XhoI
ZraI