Restriction map for
pFN21AB8134
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# NO CUT ENZYMES ( 156 enzymes) #
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AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I AspEI AspHI
AspI AviII AvrII BamHI BbeI
BbrPI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KspI MamI MfeI
MluI MroI MseI MslI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NlaIII NotI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Acc65I 671 GGTACC G^GTACC
AccI 73 GTAGAC GT^[AC][GT]AC
AlwI 780 GATCC GGATC(4/5)
AlwNI 721 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 38 GGGCCC GGGCC^C
Asp718I 671 GGTACC G^GTACC
AvaI 617 CCCGAG C^[CT]CG[GA]G
BalI 485 TGGCCA TGG^CCA
BbsI 337 GAAGAC GAAGAC(2/6)
BciVI 468 GGATAC GTATCC(6/5)
BglI 103 GCCCCCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 337 GAAGAC GAAGAC(2/6)
BsaI 644 GAGACC GGTCTC(1/5)
BsmAI 644 GAGAC GTCTC(1/5)
BsoBI 617 CCCGAG C^[CT]CG[GA]G
BstYI 779 AGATCC [GA]^GATC[CT]
CfoI 774 GCGC GCG^C
DpnII 780 GATC ^GATC
DsaI 767 CCGTGG C^C[GA][CT]GG
EaeI 485 TGGCCA [CT]^GGCC[GA]
EarI 639 GAAGAG CTCTTC(1/4)
EcoNI 312 CCTCAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 126 CCTTGG C^C[AT][AT]GG
HhaI 774 GCGC GCG^C
HinP1I 774 GCGC G^CGC
KpnI 671 GGTACC GGTAC^C
Ksp632I 639 GAAGAG CTCTTC(1/4)
MaeII 788 ACGT A^CGT
MaeIII 3 GTCAC ^GT[ACGT]AC
MboI 780 GATC ^GATC
MflI 779 AGATCC [GA]^GATC[CT]
MluNI 485 TGGCCA TGG^CCA
MscI 485 TGGCCA TGG^CCA
MspA1I 679 CAGCGG C[AC]G^C[GT]G
NdeII 780 GATC ^GATC
NruI 741 TCGCGA TCG^CGA
PleI 763 GAGTC GAGTC(4/5)
Sau3AI 780 GATC ^GATC
SmlI 313 CTCAAG C^T[CT][GA]AG
StyI 126 CCTTGG C^C[AT][AT]GG
TaqI 806 TCGA T^CGA
TfiI 738 GAATC G^A[AT]TC
Tsp45I 3 GTCAC ^GT[GC]AC
TspRI 155 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 806 TCGA T^CGA
XhoII 779 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccII 742 CGCG CG^CG
775 CGCG
AfaI 672 GTAC GT^AC
786 GTAC
BanI 256 GGCACC G^G[CT][GA]CC
671 GGTACC
BbvI 552 GCAGC GCAGC(8/12)
842 GCTGC
BfaI 53 CTAG C^TAG
349 CTAG
BsmFI 623 GGGAC GGGAC(10/14)
429 GTCCC
BspMI 797 ACCTGC ACCTGC(4/8)
175 GCAGGT
BstUI 742 CGCG CG^CG
775 CGCG
DdeI 203 CTGAG C^T[ACGT]AG
667 CTGAG
HinfI 738 GAATC G^A[ACGT]TC
763 GAGTC
HphI 178 GGTGA GGTGA(8/7)
697 GGTGA
MaeI 53 CTAG C^TAG
349 CTAG
MvnI 742 CGCG CG^CG
775 CGCG
PflMI 49 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
720 CCAGGGGCTGG
RsaI 672 GTAC GT^AC
786 GTAC
SfaNI 352 GATGC GCATC(5/9)
528 GATGC
TseI 552 GCAGC G^C[AT]GC
842 GCTGC
Van91I 49 CCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
720 CCAGGGGCTGG
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AvaII BanII BcnI
BmyI BpmI BsaJI BseRI BsiYI
BslI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HpaII ItaI MboII MnlI
MspI MvaI MwoI NciI NlaIV
PpuMI Sau96I ScrFI