(Circular) (Six-base) MAPSORT of: pFN21AB8134.seq Check: 9081 from: 1 to: 5551 pFN21AB8134 5551 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2171 2171 Size: 5551 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3361 278 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2079 2894 1937 Size: 142 815 4594 Fragments arranged by size: 4594 815 142 Acc65I G'GTAC_C Cuts at: 2676 2872 2676 Size: 196 5355 AclI AA'CG_TT Cuts at: 1563 3678 4051 1563 Size: 2115 373 3063 Fragments arranged by size: 3063 2115 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2774 3614 4998 5266 1632 Size: 1142 840 1384 268 1917 Fragments arranged by size: 1917 1384 1142 840 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5354 AflIII A'CryG_T Cuts at: 1203 4450 5427 1203 Size: 3247 977 1327 Fragments arranged by size: 3247 1327 977 AgeI A'CCGG_T Cuts at: 4382 4382 Size: 5551 AhdI GACnn_n'nnGTC Cuts at: 4280 4280 Size: 5551 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5551 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5551 AlwNI CAG_nnn'CTG Cuts at: 2731 3294 4866 2731 Size: 563 1572 3416 Fragments arranged by size: 3416 1572 563 ApaI G_GGCC'C Cuts at: 2047 2047 Size: 5551 ApaLI G'TGCA_C Cuts at: 3608 4764 3608 Size: 1156 4395 ApoI r'AATT_y Cuts at: 1493 2860 3086 3238 5347 1493 Size: 1367 226 152 2109 1697 Fragments arranged by size: 2109 1697 1367 226 152 AseI AT'TA_AT Cuts at: 160 1032 4105 160 Size: 872 3073 1606 Fragments arranged by size: 3073 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 AvaI C'yCGr_G Cuts at: 1870 1943 2622 2876 1870 Size: 73 679 254 4545 Fragments arranged by size: 4545 679 254 73 BamHI G'GATC_C Cuts at: 2881 3248 2881 Size: 367 5184 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2261 2676 2872 4327 618 Size: 325 217 627 474 415 196 1455 1842 Fragments arranged by size: 1842 1455 627 474 415 325 217 196 BanII G_rGCy'C Cuts at: 1391 2047 2143 2175 2870 1391 Size: 656 96 32 695 4072 Fragments arranged by size: 4072 695 656 96 32 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2349 928 Size: 971 450 4130 Fragments arranged by size: 4130 971 450 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3742 3776 1903 Size: 34 1805 34 3678 Fragments arranged by size: 3678 1805 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2467 3443 4653 2467 Size: 976 1210 3365 Fragments arranged by size: 3365 1210 976 BclI T'GATC_A Cuts at: 1548 1548 Size: 5551 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2114 4162 136 Size: 107 122 71 631 605 442 2048 1525 Fragments arranged by size: 2048 1525 631 605 442 122 107 71 BglII A'GATC_T Cuts at: 5546 5546 Size: 5551 BlpI GC'TnA_GC Cuts at: 2968 2968 Size: 5551 Bme1580I G_kGCm'C Cuts at: 1790 2047 3612 4768 1790 Size: 257 1565 1156 2573 Fragments arranged by size: 2573 1565 1156 257 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5551 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3282 4240 442 Size: 416 53 83 399 24 1865 958 1753 Fragments arranged by size: 1865 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2165 2261 2732 2753 4211 1239 Size: 812 114 96 471 21 1458 2579 Fragments arranged by size: 2579 1458 812 471 114 96 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2303 3676 4541 4839 2303 Size: 1373 865 298 3015 Fragments arranged by size: 3015 1373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2643 4214 882 Size: 617 1144 1571 2219 Fragments arranged by size: 2219 1571 1144 617 BsaAI yAC'GTr Cuts at: 493 1204 3342 493 Size: 711 2138 2702 Fragments arranged by size: 2702 2138 711 BsaBI GATnn'nnATC Cuts at: 5545 5545 Size: 5551 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3358 3740 4375 275 Size: 53 83 186 936 1825 382 635 1451 Fragments arranged by size: 1825 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3984 4382 4656 4803 5214 3984 Size: 398 274 147 411 4321 Fragments arranged by size: 4321 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2705 2735 1178 Size: 30 165 30 1302 30 3994 Fragments arranged by size: 3994 1302 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2306 2556 1186 Size: 1036 84 250 4181 Fragments arranged by size: 4181 1036 250 84 BseYI C'CCAG_C Cuts at: 2053 2065 2457 4754 5250 2053 Size: 12 392 2297 496 2354 Fragments arranged by size: 2354 2297 496 392 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4978 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3001 3762 4790 1540 Size: 275 186 1000 761 1028 2301 Fragments arranged by size: 2301 1028 1000 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3612 3697 4768 3612 Size: 85 1071 4395 Fragments arranged by size: 4395 1071 85 BsmI GAATG_Cn' Cuts at: 3062 3155 3062 Size: 93 5458 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2047 2143 2175 2870 3612 3697 4768 Size: 399 257 96 32 695 742 85 1071 Cuts at: 4768 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 695 399 257 96 85 32 BspEI T'CCGG_A Cuts at: 5214 5214 Size: 5551 BspHI T'CATG_A Cuts at: 3440 5170 3440 Size: 1730 3821 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2171 2811 2906 844 Size: 1327 640 95 3489 Fragments arranged by size: 3489 1327 640 95 BsrBI CCG'CTC Cuts at: 1394 1823 3438 1394 Size: 429 1615 3507 Fragments arranged by size: 3507 1615 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4046 4220 65 Size: 1183 2798 174 1396 Fragments arranged by size: 2798 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4195 4382 1700 Size: 110 2385 187 2869 Fragments arranged by size: 2869 2385 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5551 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5551 BssSI C'ACGA_G Cuts at: 3611 4623 3611 Size: 1012 4539 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5551 BstBI TT'CG_AA Cuts at: 4355 4355 Size: 5551 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5551 BstYI r'GATC_y Cuts at: 1972 2784 2881 3248 3633 3650 5102 5188 5200 Size: 812 97 367 385 17 1452 86 12 Cuts at: 5200 5546 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 812 385 367 346 97 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2772 513 Size: 552 1707 3292 Fragments arranged by size: 3292 1707 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4812 Fragments arranged by size: 4812 683 56 BtsI GCAGTG_nn' Cuts at: 3067 3861 3881 3067 Size: 794 20 4737 Fragments arranged by size: 4737 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5551 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5516 DraI TTT'AAA Cuts at: 2855 2855 Size: 5551 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4558 809 Size: 507 3242 1802 Fragments arranged by size: 3242 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2490 2998 3887 Size: 54 1425 50 275 18 660 508 889 Cuts at: 3887 5516 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 660 508 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2998 1537 Size: 275 1186 4090 Fragments arranged by size: 4090 1186 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2639 3481 1353 Size: 1286 842 3423 Fragments arranged by size: 3423 1286 842 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4138 4512 4658 387 Size: 1064 138 2549 374 146 1280 Fragments arranged by size: 2549 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2051 2165 2261 2732 2753 2774 3614 Size: 393 419 114 96 471 21 21 840 Cuts at: 3614 4211 4998 5266 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 840 787 597 471 419 393 268 114 96 21 21 EcoNI CCTnn'n_nnAGG Cuts at: 2321 2321 Size: 5551 EcoO109I rG'GnC_Cy Cuts at: 2043 2044 2128 2707 2800 2838 2043 Size: 1 84 579 93 38 4756 Fragments arranged by size: 4756 579 93 84 38 1 EcoRI G'AATT_C Cuts at: 2860 3238 2860 Size: 378 5173 FspI TGC'GCA Cuts at: 4057 4057 Size: 5551 HaeII r_GCGC'y Cuts at: 1678 4698 1678 Size: 3020 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2177 2209 2524 2556 4192 Size: 32 135 32 605 32 315 32 1636 Cuts at: 4192 4224 4266 4298 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1636 605 315 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2895 3141 669 Size: 988 281 957 246 3079 Fragments arranged by size: 3079 988 957 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5551 HpaI GTT'AAC Cuts at: 3141 3141 Size: 5551 KpnI G_GTAC'C Cuts at: 2680 2876 2680 Size: 196 5355 MfeI C'AATT_G Cuts at: 3150 3150 Size: 5551 MluI A'CGCG_T Cuts at: 5427 5427 Size: 5551 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4665 4849 1305 Size: 3360 184 2007 Fragments arranged by size: 3360 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2492 10 Size: 54 1425 343 660 3069 Fragments arranged by size: 3069 1425 660 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3509 3868 4027 518 Size: 648 2343 359 159 2042 Fragments arranged by size: 2343 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2686 2967 3644 4792 5037 1606 Size: 1080 281 677 1148 245 2120 Fragments arranged by size: 2120 1148 1080 677 281 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4999 NdeI CA'TA_TG Cuts at: 387 387 Size: 5551 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5551 NotI GC'GGCC_GC Cuts at: 2998 2998 Size: 5551 NruI TCG'CGA Cuts at: 2748 2748 Size: 5551 NspI r_CATG'y Cuts at: 1132 1229 2909 4454 1132 Size: 97 1680 1545 2229 Fragments arranged by size: 2229 1680 1545 97 PasI CC'CwG_GG Cuts at: 1348 2023 1348 Size: 675 4876 PciI A'CATG_T Cuts at: 4450 4450 Size: 5551 PflMI CCAn_nnn'nTGG Cuts at: 1108 2060 2731 1108 Size: 952 671 3928 Fragments arranged by size: 3928 952 671 PmeI GTTT'AAAC Cuts at: 2855 2855 Size: 5551 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3620 3652 5133 5165 3620 Size: 32 1481 32 4006 Fragments arranged by size: 4006 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2128 2800 2838 2128 Size: 672 38 4841 Fragments arranged by size: 4841 672 38 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5551 PsiI TTA'TAA Cuts at: 3121 3121 Size: 5551 PspOMI G'GGCC_C Cuts at: 2043 2043 Size: 5551 PstI C_TGCA'G Cuts at: 830 2903 830 Size: 2073 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5551 SalI G'TCGA_C Cuts at: 1936 2893 1936 Size: 957 4594 SbfI CC_TGCA'GG Cuts at: 2903 2903 Size: 5551 ScaI AGT'ACT Cuts at: 3799 5511 3799 Size: 1712 3839 SfcI C'TryA_G Cuts at: 826 1045 2899 4034 4715 4906 826 Size: 219 1854 1135 681 191 1471 Fragments arranged by size: 1854 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5551 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5551 SmaI CCC'GGG Cuts at: 1872 2878 1872 Size: 1006 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2318 3655 4556 4818 820 Size: 197 926 375 1337 901 262 1553 Fragments arranged by size: 1553 1337 926 901 375 262 197 SnaBI TAC'GTA Cuts at: 493 3342 493 Size: 2849 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5551 SphI G_CATG'C Cuts at: 1132 2909 1132 Size: 1777 3774 SspI AAT'ATT Cuts at: 5 52 3475 5 Size: 47 3423 2081 Fragments arranged by size: 3423 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5551 StyI C'CwwG_G Cuts at: 513 1065 2131 2990 513 Size: 552 1066 859 3074 Fragments arranged by size: 3074 1066 859 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3578 3595 3748 3933 4378 325 Size: 1255 1998 17 153 185 445 1498 Fragments arranged by size: 1998 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3797 5509 96 Size: 274 80 33 51 1443 1820 1712 138 Fragments arranged by size: 1820 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2705 2737 5511 5543 2705 Size: 32 2774 32 2713 Fragments arranged by size: 2774 2713 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5551 XbaI T'CTAG_A Cuts at: 2887 2887 Size: 5551 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 5551 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5551 XmaI C'CCGG_G Cuts at: 1870 2876 1870 Size: 1006 4545 XmnI GAAnn'nnTTC Cuts at: 3680 3680 Size: 5551 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3359 276 Size: 53 83 186 936 1825 2468 Fragments arranged by size: 2468 1825 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI