Restriction map for
pFN21AB8134
(Linear) (Six-base) MAPSORT of: KIBB8134.seq Check: 9441 from: 1 to: 846
KIBB8134.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 166 846
Size: 166 680
AccI GT'mk_AC
Cuts at: 0 74 846
Size: 74 772
Acc65I G'GTAC_C
Cuts at: 0 671 846
Size: 671 175
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 769 846
Size: 769 77
AlwNI CAG_nnn'CTG
Cuts at: 0 726 846
Size: 726 120
ApaI G_GGCC'C
Cuts at: 0 42 846
Size: 42 804
AvaI C'yCGr_G
Cuts at: 0 617 846
Size: 617 229
BanI G'GyrC_C
Cuts at: 0 256 671 846
Size: 256 415 175
Fragments arranged by size:
415 256 175
BanII G_rGCy'C
Cuts at: 0 42 138 170 846
Size: 42 96 32 676
Fragments arranged by size:
676 96 42 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 344 846
Size: 344 502
BciVI GTATCCnnnnn_n'
Cuts at: 0 462 846
Size: 462 384
BglI GCCn_nnn'nGGC
Cuts at: 0 109 846
Size: 109 737
Bme1580I G_kGCm'C
Cuts at: 0 42 846
Size: 42 804
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 160 256 727 748 846
Size: 46 114 96 471 21 98
Fragments arranged by size:
471 114 98 96 46 21
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 846
Size: 298 548
BsaI GGTCTCn'nnnn_
Cuts at: 0 638 846
Size: 638 208
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 700 730 846
Size: 700 30 116
Fragments arranged by size:
700 116 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 217 301 551 846
Size: 217 84 250 295
Fragments arranged by size:
295 250 217 84
BseYI C'CCAG_C
Cuts at: 0 48 60 452 846
Size: 48 12 392 394
Fragments arranged by size:
394 392 48 12
Bsp1286I G_dGCh'C
Cuts at: 0 42 138 170 846
Size: 42 96 32 676
Fragments arranged by size:
676 96 42 32
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 166 806 846
Size: 166 640 40
Fragments arranged by size:
640 166 40
BstYI r'GATC_y
Cuts at: 0 779 846
Size: 779 67
BtgI C'CryG_G
Cuts at: 0 767 846
Size: 767 79
EaeI y'GGCC_r
Cuts at: 0 485 846
Size: 485 361
EarI CTCTTCn'nnn_
Cuts at: 0 634 846
Size: 634 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 46 160 256 727 748 769 846
Size: 46 114 96 471 21 21 77
Fragments arranged by size:
471 114 96 77 46 21 21
EcoNI CCTnn'n_nnAGG
Cuts at: 0 316 846
Size: 316 530
EcoO109I rG'GnC_Cy
Cuts at: 0 38 39 123 702 795 833 846
Size: 38 1 84 579 93 38 13
Fragments arranged by size:
579 93 84 38 38 13 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 519 551 846
Size: 172 32 315 32 295
Fragments arranged by size:
315 295 172 32 32
KpnI G_GTAC'C
Cuts at: 0 675 846
Size: 675 171
MscI TGG'CCA
Cuts at: 0 487 846
Size: 487 359
MspA1I CmG'CkG
Cuts at: 0 681 846
Size: 681 165
NruI TCG'CGA
Cuts at: 0 743 846
Size: 743 103
PasI CC'CwG_GG
Cuts at: 0 18 846
Size: 18 828
PflMI CCAn_nnn'nTGG
Cuts at: 0 55 726 846
Size: 55 671 120
Fragments arranged by size:
671 120 55
PpuMI rG'GwC_Cy
Cuts at: 0 123 795 833 846
Size: 123 672 38 13
Fragments arranged by size:
672 123 38 13
PspOMI G'GGCC_C
Cuts at: 0 38 846
Size: 38 808
SmlI C'TyrA_G
Cuts at: 0 313 846
Size: 313 533
StyI C'CwwG_G
Cuts at: 0 126 846
Size: 126 720
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 700 732 846
Size: 700 32 114
Fragments arranged by size:
700 114 32
Enzymes that do cut:
AarI AccI Acc65I AcuI AlwNI ApaI AvaI BanI
BanII BbsI BciVI BglI Bme1580I BpmI BpuEI BsaI
BsaXI BseRI BseYI Bsp1286I BspMI BstYI BtgI EaeI
EarI Eco57MI EcoNI EcoO109I Hin4I KpnI MscI MspA1I
NruI PasI PflMI PpuMI PspOMI SmlI StyI TstI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BclI BfrBI BglII
BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaBI
BsaHI BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI BtsI
ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII
HindIII HpaI KasI MfeI MluI MmeI MslI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI PvuII RsrII SacI SacII
SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI SwaI TaqII TatI Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI