Restriction map for
pFN21AB8133
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AccIII AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
AspEI AspHI AvrII BamHI BbrPI
BciVI BclI BfrI BlnI BlpI
Bpu1102I BsaWI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstXI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DraIII Eam1105I EarI Eco47III Eco81I
EcoNI EcoRI EcoT22I FbaI FseI
HindIII HpaI Ksp632I KspI MfeI
MluI MroI MseI MunI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp509I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 33 enzymes) #
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AatII 1432 GACGTC GACGT^C
AccI 40 GTCTAC GT^[AC][GT]AC
AflIII 569 ACGTGT A^C[GA][CT]GT
AviII 1383 TGCGCA TGC^GCA
BbeI 1033 GGCGCC GGCGC^C
BbsI 1549 GTCTTC GAAGAC(2/6)
BbvCI 291 CCTCAGC CCTCAGC(-5/-2)
BglI 1453 GCCAGCGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1549 GTCTTC GAAGAC(2/6)
BsaAI 1252 TACGTG [CT]AC^GT[GA]
BsaBI 1092 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 68 CGGCCG CG[GA][CT]^CG
BssSI 1525 CACGAG CACGAG(-5/-1)
BstEII 808 GGTGACC G^GT[ACGT]ACC
BstPI 808 GGTGACC G^GT[ACGT]ACC
DrdI 31 GACAGCCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 68 CGGCCG C^GGCCG
EclXI 68 CGGCCG C^GGCCG
Eco52I 68 CGGCCG C^GGCCG
EcoO65I 808 GGTGACC G^GT[ACGT]ACC
EcoRV 631 GATATC GAT^ATC
FspI 1383 TGCGCA TGC^GCA
KasI 1033 GGCGCC G^GCGCC
MaeIII 809 GTGAC ^GT[ACGT]AC
MamI 1092 GATCGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1033 GGCGCC GG^CGCC
PpuMI 281 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 949 CAGCTG CAG^CTG
SfoI 1033 GGCGCC GGC^GCC
SmlI 1348 CTCAAG C^T[CT][GA]AG
SphI 962 GCATGC GCATG^C
Tsp45I 809 GTGAC ^GT[GC]AC
XcmI 1274 CCAACGGTGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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Acc65I 311 GGTACC G^GTACC
1244 GGTACC
AlwI 720 GATCC GGATC(4/5)
1107 GATCC
ApaI 63 GGGCCC GGGCC^C
1135 GGGCCC
Asp718I 311 GGTACC G^GTACC
1244 GGTACC
AspI 277 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1186 GACCACGTC
BalI 1451 TGGCCA TGG^CCA
1511 TGGCCA
BfaI 523 CTAG C^TAG
967 CTAG
BglII 47 AGATCT A^GATCT
350 AGATCT
BsaI 592 GAGACC GGTCTC(1/5)
970 GAGACC
BsmAI 592 GAGAC GTCTC(1/5)
970 GAGAC
BsrFI 805 GCCGGT [GA]^CCGG[CT]
1469 ACCGGC
Cfr10I 805 GCCGGT [GA]^CCGG[CT]
1469 ACCGGC
DraII 281 AGGTCCT [GA]G^G[ACGT]CC[CT]
1134 AGGGCCC
DsaI 1127 CCACGG C^C[GA][CT]GG
1654 CCACGG
EcoO109I 281 AGGTCCT [GA]G^G[ACGT]CC[CT]
1134 AGGGCCC
HaeII 1033 GGCGCC [GA]GCGC^[CT]
1613 GGCGCT
HincII 694 GTCAAC GT[CT]^[GA]AC
1537 GTCAAC
HindII 694 GTCAAC GT[CT]^[GA]AC
1537 GTCAAC
KpnI 311 GGTACC GGTAC^C
1244 GGTACC
MaeI 523 CTAG C^TAG
967 CTAG
MluNI 1451 TGGCCA TGG^CCA
1511 TGGCCA
MscI 1451 TGGCCA TGG^CCA
1511 TGGCCA
NspI 395 ACATGC [GA]CATG^[CT]
962 GCATGC
PflFI 277 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1186 GACCACGTC
PflMI 197 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCAGGATGTGG
PstI 768 CTGCAG CTGCA^G
1519 CTGCAG
SexAI 161 ACCAGGT A^CC[AT]GGT
278 ACCAGGT
SfcI 768 CTGCAG C^T[GA][CT]AG
1519 CTGCAG
Tth111I 277 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1186 GACCACGTC
Van91I 197 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
672 CCAGGATGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwNI AvaI AvaII BanI BanII
BbvI BcgI BcnI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EaeI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MflI MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI StyI
TaqI TseI TspRI TthHB8I XhoII