(Circular) (Six-base) MAPSORT of: pFN21AB8133.seq Check: 8222 from: 1 to: 6403 pFN21AB8133 6403 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3441 4213 278 Size: 53 83 186 936 1905 772 2468 Fragments arranged by size: 2468 1905 936 772 186 83 53 AccI GT'mk_AC Cuts at: 1937 2046 3746 1937 Size: 109 1700 4594 Fragments arranged by size: 4594 1700 109 Acc65I G'GTAC_C Cuts at: 2316 3249 3724 2316 Size: 933 475 4995 Fragments arranged by size: 4995 933 475 AclI AA'CG_TT Cuts at: 1563 4530 4903 1563 Size: 2967 373 3063 Fragments arranged by size: 3063 2967 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3541 4466 5850 6118 1632 Size: 1909 925 1384 268 1917 Fragments arranged by size: 1917 1909 1384 925 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6206 AflIII A'CryG_T Cuts at: 1203 2574 5302 6279 1203 Size: 1371 2728 977 1327 Fragments arranged by size: 2728 1371 1327 977 AgeI A'CCGG_T Cuts at: 5234 5234 Size: 6403 AhdI GACnn_n'nnGTC Cuts at: 5132 5132 Size: 6403 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6403 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2042 1184 Size: 858 5545 AlwNI CAG_nnn'CTG Cuts at: 2230 2266 2290 3115 3577 4146 5718 2230 Size: 36 24 825 462 569 1572 2915 Fragments arranged by size: 2915 1572 825 569 462 36 24 ApaI G_GGCC'C Cuts at: 2072 3144 2072 Size: 1072 5331 ApaLI G'TGCA_C Cuts at: 4460 5616 4460 Size: 1156 5247 ApoI r'AATT_y Cuts at: 1493 3712 3938 4090 6199 1493 Size: 2219 226 152 2109 1697 Fragments arranged by size: 2219 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4957 160 Size: 872 3925 1606 Fragments arranged by size: 3925 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 AvaI C'yCGr_G Cuts at: 1870 1943 2470 2611 3020 3728 1870 Size: 73 527 141 409 708 4545 Fragments arranged by size: 4545 708 527 409 141 73 BamHI G'GATC_C Cuts at: 3733 4100 3733 Size: 367 6036 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2242 2316 3038 3249 3649 Size: 325 217 627 455 74 722 211 400 Cuts at: 3649 3724 5179 618 Size: 75 1455 1842 Fragments arranged by size: 1842 1455 722 627 455 400 325 217 211 75 74 BanII G_rGCy'C Cuts at: 1391 2072 3144 3167 3382 3722 1391 Size: 681 1072 23 215 340 4072 Fragments arranged by size: 4072 1072 681 340 215 23 BbeI G_GCGC'C Cuts at: 3042 3042 Size: 6403 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3547 928 Size: 971 1648 3784 Fragments arranged by size: 3784 1648 971 BbvCI CC'TCA_GC Cuts at: 2297 2297 Size: 6403 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2764 2798 3011 3045 3433 3467 4594 Size: 34 827 34 213 34 388 34 1127 Cuts at: 4594 4628 1903 Size: 34 3678 Fragments arranged by size: 3678 1127 827 388 213 34 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4295 5505 4295 Size: 1210 5193 BclI T'GATC_A Cuts at: 1548 1548 Size: 6403 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3464 5014 136 Size: 107 122 71 631 605 1792 1550 1525 Fragments arranged by size: 1792 1550 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2052 2355 6398 2052 Size: 303 4043 2057 Fragments arranged by size: 4043 2057 303 BlpI GC'TnA_GC Cuts at: 3820 3820 Size: 6403 Bme1580I G_kGCm'C Cuts at: 1790 2072 2187 2247 3144 4464 5620 1790 Size: 282 115 60 897 1320 1156 2573 Fragments arranged by size: 2573 1320 1156 897 282 115 60 BmgBI CAC'GTC Cuts at: 1318 2080 2182 2575 2826 3196 1318 Size: 762 102 393 251 370 4525 Fragments arranged by size: 4525 762 393 370 251 102 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2422 2431 4134 Size: 416 53 83 399 24 1005 9 1703 Cuts at: 4134 5092 442 Size: 958 1753 Fragments arranged by size: 1753 1703 1005 958 416 399 83 53 24 9 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6403 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3396 3396 Size: 6403 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2380 2437 2954 3629 5063 1239 Size: 1141 57 517 675 1434 2579 Fragments arranged by size: 2579 1434 1141 675 517 57 Bpu10I CC'TnA_GC Cuts at: 2060 2297 2060 Size: 237 6166 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3338 4528 5393 5691 3338 Size: 1190 865 298 4050 Fragments arranged by size: 4050 1190 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2591 2969 5066 882 Size: 617 1092 378 2097 2219 Fragments arranged by size: 2219 2097 1092 617 378 BsaAI yAC'GTr Cuts at: 493 1204 3259 4194 493 Size: 711 2055 935 2702 Fragments arranged by size: 2702 2055 935 711 BsaBI GATnn'nnATC Cuts at: 3101 6397 3101 Size: 3296 3107 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3039 3438 3600 4210 Size: 53 83 186 936 1506 399 162 610 Cuts at: 4210 4592 5227 275 Size: 382 635 1451 Fragments arranged by size: 1506 1451 936 635 610 399 382 186 162 83 53 BsaWI w'CCGG_w Cuts at: 4836 5234 5508 5655 6066 4836 Size: 398 274 147 411 5173 Fragments arranged by size: 5173 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6178 Fragments arranged by size: 6178 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2231 2488 2879 3341 1186 Size: 1045 257 391 462 4248 Fragments arranged by size: 4248 1045 462 391 257 BseYI C'CCAG_C Cuts at: 2194 3148 5606 6102 2194 Size: 954 2458 496 2495 Fragments arranged by size: 2495 2458 954 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2276 2279 2329 2372 2606 1163 Size: 573 540 3 50 43 234 4960 Fragments arranged by size: 4960 573 540 234 50 43 3 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2076 3853 4614 5642 1540 Size: 275 186 75 1777 761 1028 2301 Fragments arranged by size: 2301 1777 1028 761 275 186 75 BsiHKAI G_wGCw'C Cuts at: 4464 4549 5620 4464 Size: 85 1071 5247 Fragments arranged by size: 5247 1071 85 BsmI GAATG_Cn' Cuts at: 3914 4007 3914 Size: 93 6310 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2187 2247 3144 3167 3382 3722 Size: 399 282 115 60 897 23 215 340 Cuts at: 3722 4464 4549 5620 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 897 742 399 340 282 215 115 85 60 23 BspEI T'CCGG_A Cuts at: 6066 6066 Size: 6403 BspHI T'CATG_A Cuts at: 4292 6022 4292 Size: 1730 4673 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2628 2805 3345 3758 844 Size: 1784 177 540 413 3489 Fragments arranged by size: 3489 1784 540 413 177 BsrBI CCG'CTC Cuts at: 1394 1823 4290 1394 Size: 429 2467 3507 Fragments arranged by size: 3507 2467 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4898 5072 65 Size: 1183 3650 174 1396 Fragments arranged by size: 3650 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2810 3474 5047 5234 1700 Size: 110 1000 664 1573 187 2869 Fragments arranged by size: 2869 1573 1000 664 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6403 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6403 BssSI C'ACGA_G Cuts at: 3530 4463 5475 3530 Size: 933 1012 4458 Fragments arranged by size: 4458 1012 933 BstAPI GCAn_nnn'nTGC Cuts at: 794 2266 3115 3577 794 Size: 1472 849 462 3620 Fragments arranged by size: 3620 1472 849 462 BstBI TT'CG_AA Cuts at: 5207 5207 Size: 6403 BstEII G'GTnAC_C Cuts at: 1184 2813 1184 Size: 1629 4774 BstYI r'GATC_y Cuts at: 1972 2052 2355 2724 3111 3733 4100 4485 4502 Size: 80 303 369 387 622 367 385 17 Cuts at: 4502 5954 6040 6052 6398 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 622 387 385 369 367 346 303 86 80 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3132 3659 513 Size: 552 939 1128 527 3257 Fragments arranged by size: 3257 1128 939 552 527 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5664 Fragments arranged by size: 5664 683 56 BtsI GCAGTG_nn' Cuts at: 3034 3919 4713 4733 3034 Size: 885 794 20 4704 Fragments arranged by size: 4704 885 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6403 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6368 DraI TTT'AAA Cuts at: 3707 3707 Size: 6403 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2042 5410 809 Size: 507 726 3368 1802 Fragments arranged by size: 3368 1802 726 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2073 2085 2199 Size: 54 1425 50 275 18 243 12 114 Cuts at: 2199 2766 2820 2985 3294 3456 3516 3588 3850 Size: 567 54 165 309 162 60 72 262 Cuts at: 3850 4739 6368 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 567 309 275 262 243 165 162 114 72 60 54 54 50 43 18 12 EagI C'GGCC_G Cuts at: 1537 1812 2073 3850 1537 Size: 275 261 1777 4090 Fragments arranged by size: 4090 1777 275 261 EarI CTCTTCn'nnn_ Cuts at: 1353 4333 1353 Size: 2980 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3482 4990 5364 5510 387 Size: 1064 138 1893 1508 374 146 1280 Fragments arranged by size: 1893 1508 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2380 2437 2954 3541 3629 4466 5063 Size: 393 748 57 517 587 88 837 597 Cuts at: 5063 5850 6118 1239 Size: 787 268 1524 Fragments arranged by size: 1524 837 787 748 597 587 517 393 268 88 57 EcoO109I rG'GnC_Cy Cuts at: 2287 3140 2287 Size: 853 5550 EcoRI G'AATT_C Cuts at: 3712 4090 3712 Size: 378 6025 EcoRV GAT'ATC Cuts at: 2638 2638 Size: 6403 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2923 2923 Size: 6403 FspI TGC'GCA Cuts at: 3390 4909 3390 Size: 1519 4884 FspAI rTGC'GCAy Cuts at: 3390 3390 Size: 6403 HaeII r_GCGC'y Cuts at: 1678 3042 3622 5550 1678 Size: 1364 580 1928 2531 Fragments arranged by size: 2531 1928 1364 580 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2627 2659 2672 2704 3204 Size: 32 135 32 1055 32 13 32 500 Cuts at: 3204 3236 5044 5076 5118 5150 1373 Size: 32 1808 32 42 32 2626 Fragments arranged by size: 2626 1808 1055 500 135 42 32 32 32 32 32 32 32 13 HincII GTy'rAC Cuts at: 669 1657 1938 2701 3544 3747 3993 669 Size: 988 281 763 843 203 246 3079 Fragments arranged by size: 3079 988 843 763 281 246 203 HindIII A'AGCT_T Cuts at: 748 748 Size: 6403 HpaI GTT'AAC Cuts at: 3993 3993 Size: 6403 KasI G'GCGC_C Cuts at: 3038 3038 Size: 6403 KpnI G_GTAC'C Cuts at: 2320 3253 3728 2320 Size: 933 475 4995 Fragments arranged by size: 4995 933 475 MfeI C'AATT_G Cuts at: 4002 4002 Size: 6403 MluI A'CGCG_T Cuts at: 6279 6279 Size: 6403 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2940 5517 5701 1305 Size: 1635 2577 184 2007 Fragments arranged by size: 2577 2007 1635 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3458 3518 10 Size: 54 1425 343 1626 60 2895 Fragments arranged by size: 2895 1626 1425 343 60 54 MslI CAynn'nnrTG Cuts at: 518 1166 2759 3224 3534 4361 4720 4879 518 Size: 648 1593 465 310 827 359 159 2042 Fragments arranged by size: 2042 1593 827 648 465 359 310 159 MspA1I CmG'CkG Cuts at: 1606 2198 2227 2956 3462 3819 4496 5644 5889 Size: 592 29 729 506 357 677 1148 245 Cuts at: 5889 1606 Size: 2120 Fragments arranged by size: 2120 1148 729 677 592 506 357 245 29 NarI GG'CG_CC Cuts at: 3039 3039 Size: 6403 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4912 Fragments arranged by size: 4912 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6403 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6403 NotI GC'GGCC_GC Cuts at: 3850 3850 Size: 6403 NspI r_CATG'y Cuts at: 1132 1229 2404 2971 3761 5306 1132 Size: 97 1175 567 790 1545 2229 Fragments arranged by size: 2229 1545 1175 790 567 97 PasI CC'CwG_GG Cuts at: 1348 3001 1348 Size: 1653 4750 PciI A'CATG_T Cuts at: 5302 5302 Size: 6403 PflMI CCAn_nnn'nTGG Cuts at: 1108 2208 2683 1108 Size: 1100 475 4828 Fragments arranged by size: 4828 1100 475 PfoI T'CCnGG_A Cuts at: 2676 3432 2676 Size: 756 5647 PmeI GTTT'AAAC Cuts at: 3707 3707 Size: 6403 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4472 4504 5985 6017 4472 Size: 32 1481 32 4858 Fragments arranged by size: 4858 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2287 2287 Size: 6403 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6403 PsiI TTA'TAA Cuts at: 3973 3973 Size: 6403 PspOMI G'GGCC_C Cuts at: 2068 3140 2068 Size: 1072 5331 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2204 2236 2204 Size: 32 6371 PstI C_TGCA'G Cuts at: 830 2777 3528 3755 830 Size: 1947 751 227 3478 Fragments arranged by size: 3478 1947 751 227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6403 PvuII CAG'CTG Cuts at: 2956 2956 Size: 6403 SalI G'TCGA_C Cuts at: 1936 3745 1936 Size: 1809 4594 SbfI CC_TGCA'GG Cuts at: 3755 3755 Size: 6403 ScaI AGT'ACT Cuts at: 4651 6363 4651 Size: 1712 4691 SexAI A'CCwGG_T Cuts at: 2166 2283 2166 Size: 117 6286 SfcI C'TryA_G Cuts at: 826 1045 2773 3524 3751 4886 5567 5758 826 Size: 219 1728 751 227 1135 681 191 1471 Fragments arranged by size: 1728 1471 1135 751 681 227 219 191 SfoI GGC'GCC Cuts at: 3040 3040 Size: 6403 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6403 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6403 SmaI CCC'GGG Cuts at: 1872 3730 1872 Size: 1858 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3353 4507 5408 5670 820 Size: 197 926 1410 1154 901 262 1553 Fragments arranged by size: 1553 1410 1154 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4194 493 Size: 3701 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6403 SphI G_CATG'C Cuts at: 1132 2971 3761 1132 Size: 1839 790 3774 Fragments arranged by size: 3774 1839 790 SspI AAT'ATT Cuts at: 5 52 4327 5 Size: 47 4275 2081 Fragments arranged by size: 4275 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6403 StyI C'CwwG_G Cuts at: 513 1065 2004 2337 2898 2979 3842 513 Size: 552 939 333 561 81 863 3074 Fragments arranged by size: 3074 939 863 561 552 333 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2208 2441 3140 4430 4447 4600 4785 Size: 1255 628 233 699 1290 17 153 185 Cuts at: 4785 5230 325 Size: 445 1498 Fragments arranged by size: 1498 1290 1255 699 628 445 233 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4649 6361 96 Size: 274 80 33 51 1443 2672 1712 138 Fragments arranged by size: 2672 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6363 6395 6363 Size: 32 6371 Tth111I GACn'n_nGTC Cuts at: 1316 2285 3194 1316 Size: 969 909 4525 Fragments arranged by size: 4525 969 909 XbaI T'CTAG_A Cuts at: 3739 3739 Size: 6403 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3286 3286 Size: 6403 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6403 XmaI C'CCGG_G Cuts at: 1870 3728 1870 Size: 1858 4545 XmnI GAAnn'nnTTC Cuts at: 4532 4532 Size: 6403 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3439 4211 276 Size: 53 83 186 936 1905 772 2468 Fragments arranged by size: 2468 1905 936 772 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI NaeI NgoMIV NruI NsiI PacI PmlI PspXI RsrII SacI SacII SanDI SapI SfiI SrfI SwaI