(Circular) (Six-base) MAPSORT of: pFN21AB8132.seq Check: 4251 from: 1 to: 6235 pFN21AB8132 6235 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4045 278 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2172 3578 1937 Size: 235 1406 4594 Fragments arranged by size: 4594 1406 235 Acc65I G'GTAC_C Cuts at: 3556 3556 Size: 6235 AclI AA'CG_TT Cuts at: 1563 4362 4735 1563 Size: 2799 373 3063 Fragments arranged by size: 3063 2799 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2672 2741 3343 4298 5682 5950 1632 Size: 1040 69 602 955 1384 268 1917 Fragments arranged by size: 1917 1384 1040 955 602 268 69 AfeI AGC'GCT Cuts at: 2866 3323 2866 Size: 457 5778 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6038 AflIII A'CryG_T Cuts at: 1203 5134 6111 1203 Size: 3931 977 1327 Fragments arranged by size: 3931 1327 977 AgeI A'CCGG_T Cuts at: 5066 5066 Size: 6235 AhdI GACnn_n'nnGTC Cuts at: 3028 4964 3028 Size: 1936 4299 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6235 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6235 AlwNI CAG_nnn'CTG Cuts at: 2203 2236 2269 2296 2650 2680 3978 5550 2203 Size: 33 33 27 354 30 1298 1572 2888 Fragments arranged by size: 2888 1572 1298 354 33 33 30 27 ApaI G_GGCC'C Cuts at: 2193 3203 3434 2193 Size: 1010 231 4994 Fragments arranged by size: 4994 1010 231 ApaLI G'TGCA_C Cuts at: 4292 5448 4292 Size: 1156 5079 ApoI r'AATT_y Cuts at: 1493 2165 3544 3770 3922 6031 1493 Size: 672 1379 226 152 2109 1697 Fragments arranged by size: 2109 1697 1379 672 226 152 AseI AT'TA_AT Cuts at: 160 1032 4789 160 Size: 872 3757 1606 Fragments arranged by size: 3757 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 AvaI C'yCGr_G Cuts at: 1870 1943 2105 3125 3149 3426 3560 1870 Size: 73 162 1020 24 277 134 4545 Fragments arranged by size: 4545 1020 277 162 134 73 24 BamHI G'GATC_C Cuts at: 3565 3932 3565 Size: 367 5868 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2542 2926 2963 3422 3556 Size: 325 217 627 755 384 37 459 134 Cuts at: 3556 5011 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 755 627 459 384 325 217 134 37 BanII G_rGCy'C Cuts at: 1391 2045 2193 2423 2435 2502 2510 3203 3434 Size: 654 148 230 12 67 8 693 231 Cuts at: 3434 3554 1391 Size: 120 4072 Fragments arranged by size: 4072 693 654 231 230 148 120 67 12 8 BbeI G_GCGC'C Cuts at: 2930 3426 2930 Size: 496 5739 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3353 3392 928 Size: 971 1454 39 3771 Fragments arranged by size: 3771 1454 971 39 BbvCI CC'TCA_GC Cuts at: 2673 2673 Size: 6235 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4426 4460 1903 Size: 34 2489 34 3678 Fragments arranged by size: 3678 2489 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4127 5337 4127 Size: 1210 5025 BclI T'GATC_A Cuts at: 1548 1548 Size: 6235 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3153 3312 4846 Size: 107 122 71 631 605 1481 159 1534 Cuts at: 4846 136 Size: 1525 Fragments arranged by size: 1534 1525 1481 631 605 159 122 107 71 BglII A'GATC_T Cuts at: 6230 6230 Size: 6235 BlpI GC'TnA_GC Cuts at: 3652 3652 Size: 6235 Bme1580I G_kGCm'C Cuts at: 1790 2193 2966 3095 3203 3434 4296 5452 1790 Size: 403 773 129 108 231 862 1156 2573 Fragments arranged by size: 2573 1156 862 773 403 231 129 108 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6235 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3113 3966 4924 Size: 416 53 83 399 24 1696 853 958 Cuts at: 4924 442 Size: 1753 Fragments arranged by size: 1753 1696 958 853 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6235 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2269 3052 3175 2269 Size: 783 123 5329 Fragments arranged by size: 5329 783 123 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2291 2306 2764 3206 4895 1239 Size: 920 132 15 458 442 1689 2579 Fragments arranged by size: 2579 1689 920 458 442 132 15 Bpu10I CC'TnA_GC Cuts at: 2673 2910 2673 Size: 237 5998 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4360 5225 5523 4360 Size: 865 298 5072 Fragments arranged by size: 5072 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 4898 882 Size: 617 603 2796 2219 Fragments arranged by size: 2796 2219 617 603 BsaAI yAC'GTr Cuts at: 493 1204 4026 493 Size: 711 2822 2702 Fragments arranged by size: 2822 2702 711 BsaBI GATnn'nnATC Cuts at: 6229 6229 Size: 6235 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2927 2940 3156 3423 Size: 53 83 186 936 1394 13 216 267 Cuts at: 3423 4042 4424 5059 275 Size: 619 382 635 1451 Fragments arranged by size: 1451 1394 936 635 619 382 267 216 186 83 53 13 BsaWI w'CCGG_w Cuts at: 2569 2730 2802 3044 4668 5066 5340 5487 5898 Size: 161 72 242 1624 398 274 147 411 Cuts at: 5898 2569 Size: 2906 Fragments arranged by size: 2906 1624 411 398 274 242 161 147 72 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 2762 2792 1178 Size: 30 165 30 606 30 723 30 4621 Fragments arranged by size: 4621 723 606 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2608 2726 2897 2987 2990 1186 Size: 1422 118 171 90 3 4431 Fragments arranged by size: 4431 1422 171 118 90 3 BseYI C'CCAG_C Cuts at: 2047 2416 2510 2528 2763 2867 3415 5438 5934 Size: 369 94 18 235 104 548 2023 496 Cuts at: 5934 2047 Size: 2348 Fragments arranged by size: 2348 2023 548 496 369 235 104 94 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5662 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3316 3685 4446 5474 1540 Size: 275 186 1315 369 761 1028 2301 Fragments arranged by size: 2301 1315 1028 761 369 275 186 BsiHKAI G_wGCw'C Cuts at: 2045 2502 3297 3381 4296 4381 5452 2045 Size: 457 795 84 915 85 1071 2828 Fragments arranged by size: 2828 1071 915 795 457 85 84 BsmI GAATG_Cn' Cuts at: 3746 3839 3746 Size: 93 6142 BsmBI CGTCTCn'nnnn_ Cuts at: 3163 3163 Size: 6235 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2193 2423 2435 2502 2510 2966 Size: 399 255 148 230 12 67 8 456 Cuts at: 2966 3095 3203 3297 3381 3434 3554 4296 4381 Size: 129 108 94 84 53 120 742 85 Cuts at: 4381 5452 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 456 399 255 230 148 129 120 108 94 85 84 67 53 12 8 BspEI T'CCGG_A Cuts at: 2569 5898 2569 Size: 3329 2906 BspHI T'CATG_A Cuts at: 4124 5854 4124 Size: 1730 4505 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3590 844 Size: 2746 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4122 1394 Size: 429 2299 3507 Fragments arranged by size: 3507 2299 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4730 4904 65 Size: 1183 3482 174 1396 Fragments arranged by size: 3482 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4879 5066 1700 Size: 110 3069 187 2869 Fragments arranged by size: 3069 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6235 BssHII G'CGCG_C Cuts at: 1925 2249 1925 Size: 324 5911 BssSI C'ACGA_G Cuts at: 3392 4295 5307 3392 Size: 903 1012 4320 Fragments arranged by size: 4320 1012 903 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6235 BstBI TT'CG_AA Cuts at: 5039 5039 Size: 6235 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6235 BstXI CCAn_nnnn'nTGG Cuts at: 2522 2522 Size: 6235 BstYI r'GATC_y Cuts at: 1972 2059 2535 2818 3565 3932 4317 4334 5786 Size: 87 476 283 747 367 385 17 1452 Cuts at: 5786 5872 5884 6230 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 747 476 385 367 346 283 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2244 2244 Size: 6235 BtgI C'CryG_G Cuts at: 513 1065 2895 3229 3307 513 Size: 552 1830 334 78 3441 Fragments arranged by size: 3441 1830 552 334 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2466 487 Size: 683 56 1240 4256 Fragments arranged by size: 4256 1240 683 56 BtsI GCAGTG_nn' Cuts at: 3751 4545 4565 3751 Size: 794 20 5421 Fragments arranged by size: 5421 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6235 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6200 DraI TTT'AAA Cuts at: 3539 3539 Size: 6235 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5242 809 Size: 507 3926 1802 Fragments arranged by size: 3926 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3313 3682 4571 Size: 54 1425 50 275 18 1483 369 889 Cuts at: 4571 6200 8 Size: 1629 43 Fragments arranged by size: 1629 1483 1425 889 369 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3313 3682 1537 Size: 275 1501 369 4090 Fragments arranged by size: 4090 1501 369 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2394 2613 2717 2976 4165 1353 Size: 1041 219 104 259 1189 3423 Fragments arranged by size: 3423 1189 1041 259 219 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3492 4822 5196 5342 387 Size: 1064 138 1903 1330 374 146 1280 Fragments arranged by size: 1903 1330 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2043 2500 2043 Size: 457 5778 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2291 2306 2672 2741 2764 3206 Size: 393 527 132 15 366 69 23 442 Cuts at: 3206 3343 4298 4895 5682 5950 1239 Size: 137 955 597 787 268 1524 Fragments arranged by size: 1524 955 787 597 527 442 393 366 268 137 132 69 23 15 EcoNI CCTnn'n_nnAGG Cuts at: 2035 2375 2035 Size: 340 5895 EcoO109I rG'GnC_Cy Cuts at: 2124 2190 2380 2518 2668 2998 3013 3199 3200 Size: 66 190 138 150 330 15 186 1 Cuts at: 3200 3430 3431 2124 Size: 230 1 4928 Fragments arranged by size: 4928 330 230 190 186 150 138 66 15 1 1 EcoRI G'AATT_C Cuts at: 3544 3922 3544 Size: 378 5857 FspI TGC'GCA Cuts at: 4741 4741 Size: 6235 HaeII r_GCGC'y Cuts at: 1678 2868 2930 3325 3426 5382 1678 Size: 1190 62 395 101 1956 2531 Fragments arranged by size: 2531 1956 1190 395 101 62 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2762 2794 2942 2974 3014 Size: 32 135 32 1190 32 148 32 40 Cuts at: 3014 3046 4876 4908 4950 4982 1373 Size: 32 1830 32 42 32 2626 Fragments arranged by size: 2626 1830 1190 148 135 42 40 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3579 3825 669 Size: 988 281 1641 246 3079 Fragments arranged by size: 3079 1641 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6235 HpaI GTT'AAC Cuts at: 3825 3825 Size: 6235 KasI G'GCGC_C Cuts at: 2926 3422 2926 Size: 496 5739 KpnI G_GTAC'C Cuts at: 3560 3560 Size: 6235 MfeI C'AATT_G Cuts at: 3834 3834 Size: 6235 MluI A'CGCG_T Cuts at: 6111 6111 Size: 6235 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3057 5349 5533 1305 Size: 1752 2292 184 2007 Fragments arranged by size: 2292 2007 1752 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4413 Fragments arranged by size: 4413 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4193 4552 4711 518 Size: 648 3027 359 159 2042 Fragments arranged by size: 3027 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2897 3231 3651 4328 5476 5721 1606 Size: 1291 334 420 677 1148 245 2120 Fragments arranged by size: 2120 1291 1148 677 420 334 245 NarI GG'CG_CC Cuts at: 2927 3423 2927 Size: 496 5739 NcoI C'CATG_G Cuts at: 513 1065 3307 513 Size: 552 2242 3441 Fragments arranged by size: 3441 2242 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6235 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6235 NotI GC'GGCC_GC Cuts at: 3682 3682 Size: 6235 NruI TCG'CGA Cuts at: 2504 2504 Size: 6235 NspI r_CATG'y Cuts at: 1132 1229 3593 5138 1132 Size: 97 2364 1545 2229 Fragments arranged by size: 2364 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2325 2933 3203 1348 Size: 977 608 270 4380 Fragments arranged by size: 4380 977 608 270 PciI A'CATG_T Cuts at: 5134 5134 Size: 6235 PflMI CCAn_nnn'nTGG Cuts at: 1108 2203 1108 Size: 1095 5140 PfoI T'CCnGG_A Cuts at: 2160 2394 2160 Size: 234 6001 PmeI GTTT'AAAC Cuts at: 3539 3539 Size: 6235 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4304 4336 5817 5849 4304 Size: 32 1481 32 4690 Fragments arranged by size: 4690 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2124 2668 3013 2124 Size: 544 345 5346 Fragments arranged by size: 5346 544 345 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6235 PsiI TTA'TAA Cuts at: 3805 3805 Size: 6235 PspOMI G'GGCC_C Cuts at: 2189 3199 3430 2189 Size: 1010 231 4994 Fragments arranged by size: 4994 1010 231 PspXI vC'TCGA_Gb Cuts at: 3149 3149 Size: 6235 PstI C_TGCA'G Cuts at: 830 3587 830 Size: 2757 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6235 SacI G_AGCT'C Cuts at: 2045 2502 2045 Size: 457 5778 SacII CC_GC'GG Cuts at: 2898 3232 2898 Size: 334 5901 SalI G'TCGA_C Cuts at: 1936 3577 1936 Size: 1641 4594 SapI GCTCTTCn'nnn_ Cuts at: 2394 2717 2976 2394 Size: 323 259 5653 Fragments arranged by size: 5653 323 259 SbfI CC_TGCA'GG Cuts at: 3587 3587 Size: 6235 ScaI AGT'ACT Cuts at: 4483 6195 4483 Size: 1712 4523 SfcI C'TryA_G Cuts at: 826 1045 3583 4718 5399 5590 826 Size: 219 2538 1135 681 191 1471 Fragments arranged by size: 2538 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2928 3424 2928 Size: 496 5739 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6235 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6235 SmaI CCC'GGG Cuts at: 1872 3428 3562 1872 Size: 1556 134 4545 Fragments arranged by size: 4545 1556 134 SmlI C'TyrA_G Cuts at: 820 1017 1943 3149 4339 5240 5502 820 Size: 197 926 1206 1190 901 262 1553 Fragments arranged by size: 1553 1206 1190 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4026 493 Size: 3533 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6235 SphI G_CATG'C Cuts at: 1132 3593 1132 Size: 2461 3774 SspI AAT'ATT Cuts at: 5 52 4159 5 Size: 47 4107 2081 Fragments arranged by size: 4107 2081 47 StuI AGG'CCT Cuts at: 1517 3266 1517 Size: 1749 4486 StyI C'CwwG_G Cuts at: 513 1065 2256 2521 3307 3674 513 Size: 552 1191 265 786 367 3074 Fragments arranged by size: 3074 1191 786 552 367 265 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2317 2630 2859 4262 4279 4432 4617 Size: 1255 737 313 229 1403 17 153 185 Cuts at: 4617 5062 325 Size: 445 1498 Fragments arranged by size: 1498 1403 1255 737 445 313 229 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4481 6193 96 Size: 274 80 33 51 1443 2504 1712 138 Fragments arranged by size: 2504 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6195 6227 6195 Size: 32 6203 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6235 XbaI T'CTAG_A Cuts at: 3571 3571 Size: 6235 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 3305 2011 Size: 1294 4941 XhoI C'TCGA_G Cuts at: 1943 3149 1943 Size: 1206 5029 XmaI C'CCGG_G Cuts at: 1870 3426 3560 1870 Size: 1556 134 4545 Fragments arranged by size: 4545 1556 134 XmnI GAAnn'nnTTC Cuts at: 3350 4364 3350 Size: 1014 5221 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4043 276 Size: 53 83 186 936 2509 2468 Fragments arranged by size: 2509 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NsiI PacI PmlI PsrI PvuII RsrII SanDI SexAI SfiI SrfI SwaI