(Circular) (Six-base) MAPSORT of: pFN21AB8129.seq Check: 2930 from: 1 to: 5728 pFN21AB8129 5728 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2104 2104 Size: 5728 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3538 278 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2773 3071 1937 Size: 836 298 4594 Fragments arranged by size: 4594 836 298 Acc65I G'GTAC_C Cuts at: 3049 3049 Size: 5728 AclI AA'CG_TT Cuts at: 1563 3855 4228 1563 Size: 2292 373 3063 Fragments arranged by size: 3063 2292 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2103 2522 3791 5175 5443 1632 Size: 471 419 1269 1384 268 1917 Fragments arranged by size: 1917 1384 1269 471 419 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5531 AflIII A'CryG_T Cuts at: 1203 2765 4627 5604 1203 Size: 1562 1862 977 1327 Fragments arranged by size: 1862 1562 1327 977 AgeI A'CCGG_T Cuts at: 4559 4559 Size: 5728 AhdI GACnn_n'nnGTC Cuts at: 2769 4457 2769 Size: 1688 4040 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5728 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5728 AlwNI CAG_nnn'CTG Cuts at: 2204 2899 2962 2999 3471 5043 2204 Size: 695 63 37 472 1572 2889 Fragments arranged by size: 2889 1572 695 472 63 37 ApaLI G'TGCA_C Cuts at: 2320 3785 4941 2320 Size: 1465 1156 3107 Fragments arranged by size: 3107 1465 1156 ApoI r'AATT_y Cuts at: 1493 2546 3037 3263 3415 5524 1493 Size: 1053 491 226 152 2109 1697 Fragments arranged by size: 2109 1697 1053 491 226 152 AseI AT'TA_AT Cuts at: 160 1032 4282 160 Size: 872 3250 1606 Fragments arranged by size: 3250 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 AvaI C'yCGr_G Cuts at: 1870 1943 2259 2751 3053 1870 Size: 73 316 492 302 4545 Fragments arranged by size: 4545 492 316 302 73 AvrII C'CTAG_G Cuts at: 2020 2020 Size: 5728 BamHI G'GATC_C Cuts at: 2016 3058 3425 2016 Size: 1042 367 4319 Fragments arranged by size: 4319 1042 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3049 4504 618 Size: 325 217 627 1262 1455 1842 Fragments arranged by size: 1842 1455 1262 627 325 217 BanII G_rGCy'C Cuts at: 1391 2177 3047 1391 Size: 786 870 4072 Fragments arranged by size: 4072 870 786 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2667 928 Size: 971 768 3989 Fragments arranged by size: 3989 971 768 BbvCI CC'TCA_GC Cuts at: 2516 2516 Size: 5728 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3919 3953 1903 Size: 34 1982 34 3678 Fragments arranged by size: 3678 1982 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3620 4830 3620 Size: 1210 4518 BclI T'GATC_A Cuts at: 1548 1548 Size: 5728 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4339 136 Size: 107 122 71 631 605 2667 1525 Fragments arranged by size: 2667 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5723 5723 Size: 5728 BlpI GC'TnA_GC Cuts at: 3145 3145 Size: 5728 Bme1580I G_kGCm'C Cuts at: 1790 2324 2452 2788 3789 4945 1790 Size: 534 128 336 1001 1156 2573 Fragments arranged by size: 2573 1156 1001 534 336 128 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5728 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2504 3459 4417 Size: 416 53 83 399 24 1087 955 958 Cuts at: 4417 442 Size: 1753 Fragments arranged by size: 1753 1087 958 955 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5728 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3025 3025 Size: 5728 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2093 2117 2265 2292 2396 2972 2976 4388 Size: 854 24 148 27 104 576 4 1412 Cuts at: 4388 1239 Size: 2579 Fragments arranged by size: 2579 1412 854 576 148 104 27 24 4 Bpu10I CC'TnA_GC Cuts at: 2516 2516 Size: 5728 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2798 2837 3853 4718 5016 2798 Size: 39 1016 865 298 3510 Fragments arranged by size: 3510 1016 865 298 39 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4391 882 Size: 617 2892 2219 Fragments arranged by size: 2892 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2768 3519 493 Size: 711 1564 751 2702 Fragments arranged by size: 2702 1564 751 711 BsaBI GATnn'nnATC Cuts at: 2048 5722 2048 Size: 3674 2054 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3535 3917 4552 275 Size: 53 83 186 936 2002 382 635 1451 Fragments arranged by size: 2002 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4161 4559 4833 4980 5391 4161 Size: 398 274 147 411 4498 Fragments arranged by size: 4498 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2238 2268 1178 Size: 30 165 30 835 30 4638 Fragments arranged by size: 4638 835 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2747 3035 1186 Size: 1561 288 3879 Fragments arranged by size: 3879 1561 288 BseYI C'CCAG_C Cuts at: 2440 2484 2933 4931 5427 2440 Size: 44 449 1998 496 2741 Fragments arranged by size: 2741 1998 496 449 44 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5155 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3178 3939 4967 1540 Size: 275 186 1177 761 1028 2301 Fragments arranged by size: 2301 1177 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2324 3789 3874 4945 2324 Size: 1465 85 1071 3107 Fragments arranged by size: 3107 1465 1071 85 BsmI GAATG_Cn' Cuts at: 3239 3332 3239 Size: 93 5635 BsmBI CGTCTCn'nnnn_ Cuts at: 2402 2402 Size: 5728 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2177 2324 2452 2788 3047 3789 3874 Size: 399 387 147 128 336 259 742 85 Cuts at: 3874 4945 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 387 336 259 147 128 85 BspEI T'CCGG_A Cuts at: 5391 5391 Size: 5728 BspHI T'CATG_A Cuts at: 2670 3617 5347 2670 Size: 947 1730 3051 Fragments arranged by size: 3051 1730 947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2104 3016 3083 844 Size: 1260 912 67 3489 Fragments arranged by size: 3489 1260 912 67 BsrBI CCG'CTC Cuts at: 1394 1823 3615 1394 Size: 429 1792 3507 Fragments arranged by size: 3507 1792 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2265 4223 4397 65 Size: 1183 1017 1958 174 1396 Fragments arranged by size: 1958 1396 1183 1017 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4372 4559 1700 Size: 110 2562 187 2869 Fragments arranged by size: 2869 2562 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5728 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5728 BssSI C'ACGA_G Cuts at: 3788 4800 3788 Size: 1012 4716 BstAPI GCAn_nnn'nTGC Cuts at: 794 2899 2962 794 Size: 2105 63 3560 Fragments arranged by size: 3560 2105 63 BstBI TT'CG_AA Cuts at: 4532 4532 Size: 5728 BstEII G'GTnAC_C Cuts at: 1184 2644 1184 Size: 1460 4268 BstXI CCAn_nnnn'nTGG Cuts at: 2656 2656 Size: 5728 BstYI r'GATC_y Cuts at: 1972 2016 2043 3058 3425 3810 3827 5279 5365 Size: 44 27 1015 367 385 17 1452 86 Cuts at: 5365 5377 5723 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1015 385 367 346 86 44 27 17 12 BtgI C'CryG_G Cuts at: 513 1065 2053 2655 513 Size: 552 988 602 3586 Fragments arranged by size: 3586 988 602 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4989 Fragments arranged by size: 4989 683 56 BtsI GCAGTG_nn' Cuts at: 3244 4038 4058 3244 Size: 794 20 4914 Fragments arranged by size: 4914 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5728 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5693 DraI TTT'AAA Cuts at: 3032 3032 Size: 5728 DraIII CAC_nnn'GTG Cuts at: 2498 2498 Size: 5728 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4735 809 Size: 507 3419 1802 Fragments arranged by size: 3419 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2206 2841 3175 Size: 54 1425 50 275 18 376 635 334 Cuts at: 3175 4064 5693 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 635 376 334 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3175 1537 Size: 275 1363 4090 Fragments arranged by size: 4090 1363 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2909 3658 1353 Size: 1556 749 3423 Fragments arranged by size: 3423 1556 749 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2911 4315 4689 4835 387 Size: 1064 138 1322 1404 374 146 1280 Fragments arranged by size: 1404 1322 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2093 2103 2117 2265 2292 2396 2522 Size: 393 461 10 14 148 27 104 126 Cuts at: 2522 2972 2976 3791 4388 5175 5443 1239 Size: 450 4 815 597 787 268 1524 Fragments arranged by size: 1524 815 787 597 461 450 393 268 148 126 104 27 14 10 4 EcoNI CCTnn'n_nnAGG Cuts at: 2024 2111 2024 Size: 87 5641 EcoO109I rG'GnC_Cy Cuts at: 2104 2129 2747 2104 Size: 25 618 5085 Fragments arranged by size: 5085 618 25 EcoRI G'AATT_C Cuts at: 3037 3415 3037 Size: 378 5350 FspI TGC'GCA Cuts at: 4234 4234 Size: 5728 HaeII r_GCGC'y Cuts at: 1678 4875 1678 Size: 3197 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2121 2153 2755 2787 4369 Size: 32 135 32 549 32 602 32 1582 Cuts at: 4369 4401 4443 4475 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1582 602 549 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2774 3072 3318 669 Size: 988 281 836 298 246 3079 Fragments arranged by size: 3079 988 836 298 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5728 HpaI GTT'AAC Cuts at: 3318 3318 Size: 5728 KpnI G_GTAC'C Cuts at: 3053 3053 Size: 5728 MfeI C'AATT_G Cuts at: 3327 3327 Size: 5728 MluI A'CGCG_T Cuts at: 5604 5604 Size: 5728 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4842 5026 1305 Size: 3537 184 2007 Fragments arranged by size: 3537 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2843 10 Size: 54 1425 343 1011 2895 Fragments arranged by size: 2895 1425 1011 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2669 2675 3686 4045 4204 518 Size: 648 1503 6 1011 359 159 2042 Fragments arranged by size: 2042 1503 1011 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2440 2896 2950 3144 3821 4969 5214 1606 Size: 834 456 54 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 834 677 456 245 194 54 NcoI C'CATG_G Cuts at: 513 1065 2655 513 Size: 552 1590 3586 Fragments arranged by size: 3586 1590 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5728 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5728 NotI GC'GGCC_GC Cuts at: 3175 3175 Size: 5728 NspI r_CATG'y Cuts at: 1132 1229 2492 3086 4631 1132 Size: 97 1263 594 1545 2229 Fragments arranged by size: 2229 1545 1263 594 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5728 PciI A'CATG_T Cuts at: 4627 4627 Size: 5728 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5728 PfoI T'CCnGG_A Cuts at: 2859 2992 2859 Size: 133 5595 PmeI GTTT'AAAC Cuts at: 3032 3032 Size: 5728 PmlI CAC'GTG Cuts at: 2768 2768 Size: 5728 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3797 3829 5310 5342 3797 Size: 32 1481 32 4183 Fragments arranged by size: 4183 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2104 2129 2104 Size: 25 5703 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5728 PsiI TTA'TAA Cuts at: 3298 3298 Size: 5728 PstI C_TGCA'G Cuts at: 830 2391 2439 2481 2967 3080 830 Size: 1561 48 42 486 113 3478 Fragments arranged by size: 3478 1561 486 113 48 42 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5728 PvuII CAG'CTG Cuts at: 2440 2896 2950 2440 Size: 456 54 5218 Fragments arranged by size: 5218 456 54 SalI G'TCGA_C Cuts at: 1936 2772 3070 1936 Size: 836 298 4594 Fragments arranged by size: 4594 836 298 SapI GCTCTTCn'nnn_ Cuts at: 2909 2909 Size: 5728 SbfI CC_TGCA'GG Cuts at: 3080 3080 Size: 5728 ScaI AGT'ACT Cuts at: 3976 5688 3976 Size: 1712 4016 SfcI C'TryA_G Cuts at: 826 1045 2387 2435 2467 2477 2963 3076 4211 Size: 219 1342 48 32 10 486 113 1135 Cuts at: 4211 4892 5083 826 Size: 681 191 1471 Fragments arranged by size: 1471 1342 1135 681 486 219 191 113 48 32 10 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5728 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5728 SmaI CCC'GGG Cuts at: 1872 2753 3055 1872 Size: 881 302 4545 Fragments arranged by size: 4545 881 302 SmlI C'TyrA_G Cuts at: 820 1017 1943 2777 2852 3832 4733 4995 820 Size: 197 926 834 75 980 901 262 1553 Fragments arranged by size: 1553 980 926 901 834 262 197 75 SnaBI TAC'GTA Cuts at: 493 3519 493 Size: 3026 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5728 SphI G_CATG'C Cuts at: 1132 2492 3086 1132 Size: 1360 594 3774 Fragments arranged by size: 3774 1360 594 SspI AAT'ATT Cuts at: 5 52 3652 5 Size: 47 3600 2081 Fragments arranged by size: 3600 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5728 StyI C'CwwG_G Cuts at: 513 1065 2020 2655 3167 513 Size: 552 955 635 512 3074 Fragments arranged by size: 3074 955 635 552 512 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2486 3755 3772 3925 4110 4555 325 Size: 1255 906 1269 17 153 185 445 1498 Fragments arranged by size: 1498 1269 1255 906 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3974 5686 96 Size: 274 80 33 51 1443 1997 1712 138 Fragments arranged by size: 1997 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2238 2270 5688 5720 2238 Size: 32 3418 32 2246 Fragments arranged by size: 3418 2246 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2288 1316 Size: 972 4756 XbaI T'CTAG_A Cuts at: 3064 3064 Size: 5728 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5728 XmaI C'CCGG_G Cuts at: 1870 2751 3053 1870 Size: 881 302 4545 Fragments arranged by size: 4545 881 302 XmnI GAAnn'nnTTC Cuts at: 3857 3857 Size: 5728 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3536 276 Size: 53 83 186 936 2002 2468 Fragments arranged by size: 2468 2002 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI