(Circular) (Six-base) MAPSORT of: pFN21AB8125.seq Check: 2286 from: 1 to: 6070 pFN21AB8125 6070 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2637 3880 278 Size: 53 83 186 936 1101 1243 2468 Fragments arranged by size: 2468 1243 1101 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2572 3261 3413 1937 Size: 635 689 152 4594 Fragments arranged by size: 4594 689 635 152 Acc65I G'GTAC_C Cuts at: 3391 3391 Size: 6070 AclI AA'CG_TT Cuts at: 1563 4197 4570 1563 Size: 2634 373 3063 Fragments arranged by size: 3063 2634 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2147 3315 4133 5517 5785 1632 Size: 515 1168 818 1384 268 1917 Fragments arranged by size: 1917 1384 1168 818 515 268 AflII C'TTAA_G Cuts at: 820 1017 2869 820 Size: 197 1852 4021 Fragments arranged by size: 4021 1852 197 AflIII A'CryG_T Cuts at: 1203 2773 4969 5946 1203 Size: 1570 2196 977 1327 Fragments arranged by size: 2196 1570 1327 977 AgeI A'CCGG_T Cuts at: 4901 4901 Size: 6070 AhdI GACnn_n'nnGTC Cuts at: 4799 4799 Size: 6070 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6070 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6070 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3315 3347 3315 Size: 32 6038 AlwNI CAG_nnn'CTG Cuts at: 2056 3813 5385 2056 Size: 1757 1572 2741 Fragments arranged by size: 2741 1757 1572 ApaLI G'TGCA_C Cuts at: 4127 5283 4127 Size: 1156 4914 ApoI r'AATT_y Cuts at: 1493 2023 2059 2285 2564 3190 3379 3605 3757 Size: 530 36 226 279 626 189 226 152 Cuts at: 3757 5866 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 626 530 279 226 226 189 152 36 AseI AT'TA_AT Cuts at: 160 1032 4624 160 Size: 872 3592 1606 Fragments arranged by size: 3592 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 AvaI C'yCGr_G Cuts at: 1870 1943 2014 3395 1870 Size: 73 71 1381 4545 Fragments arranged by size: 4545 1381 73 71 AvrII C'CTAG_G Cuts at: 2825 2825 Size: 6070 BamHI G'GATC_C Cuts at: 3400 3767 3400 Size: 367 5703 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3391 4846 618 Size: 325 217 627 1604 1455 1842 Fragments arranged by size: 1842 1604 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2213 3389 1391 Size: 822 1176 4072 Fragments arranged by size: 4072 1176 822 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3210 928 Size: 971 1311 3788 Fragments arranged by size: 3788 1311 971 BbvCI CC'TCA_GC Cuts at: 3366 3366 Size: 6070 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2646 2680 4261 4295 1903 Size: 34 709 34 1581 34 3678 Fragments arranged by size: 3678 1581 709 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3962 5172 3962 Size: 1210 4860 BclI T'GATC_A Cuts at: 1548 3154 1548 Size: 1606 4464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4681 136 Size: 107 122 71 631 605 3009 1525 Fragments arranged by size: 3009 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6065 6065 Size: 6070 BlpI GC'TnA_GC Cuts at: 3487 3487 Size: 6070 Bme1580I G_kGCm'C Cuts at: 1790 2178 4131 5287 1790 Size: 388 1953 1156 2573 Fragments arranged by size: 2573 1953 1156 388 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6070 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2065 3801 4759 Size: 416 53 83 399 24 648 1736 958 Cuts at: 4759 442 Size: 1753 Fragments arranged by size: 1753 1736 958 648 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3308 1056 Size: 2252 3818 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2913 3265 2913 Size: 352 5718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4730 1239 Size: 3491 2579 Bpu10I CC'TnA_GC Cuts at: 2212 3366 2212 Size: 1154 4916 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2368 4195 5060 5358 2368 Size: 1827 865 298 3080 Fragments arranged by size: 3080 1827 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4733 882 Size: 617 3234 2219 Fragments arranged by size: 3234 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3861 493 Size: 711 2657 2702 Fragments arranged by size: 2702 2657 711 BsaBI GATnn'nnATC Cuts at: 6064 6064 Size: 6070 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2634 3877 4259 4894 Size: 53 83 186 936 1101 1243 382 635 Cuts at: 4894 275 Size: 1451 Fragments arranged by size: 1451 1243 1101 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4503 4901 5175 5322 5733 4503 Size: 398 274 147 411 4840 Fragments arranged by size: 4840 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2952 2982 1178 Size: 30 165 30 1549 30 4266 Fragments arranged by size: 4266 1549 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2660 1186 Size: 1474 4596 BseYI C'CCAG_C Cuts at: 2832 3010 5273 5769 2832 Size: 178 2263 496 3133 Fragments arranged by size: 3133 2263 496 178 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2162 2423 1163 Size: 573 426 261 4810 Fragments arranged by size: 4810 573 426 261 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3520 4281 5309 1540 Size: 275 186 1519 761 1028 2301 Fragments arranged by size: 2301 1519 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2213 4131 4216 5287 2213 Size: 1918 85 1071 2996 Fragments arranged by size: 2996 1918 1071 85 BsmI GAATG_Cn' Cuts at: 3581 3674 3581 Size: 93 5977 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2178 2213 3389 4131 4216 5287 1391 Size: 399 388 35 1176 742 85 1071 2174 Fragments arranged by size: 2174 1176 1071 742 399 388 85 35 BspEI T'CCGG_A Cuts at: 5733 5733 Size: 6070 BspHI T'CATG_A Cuts at: 2262 2637 3959 5689 2262 Size: 375 1322 1730 2643 Fragments arranged by size: 2643 1730 1322 375 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2118 3425 844 Size: 1274 1307 3489 Fragments arranged by size: 3489 1307 1274 BsrBI CCG'CTC Cuts at: 1394 1823 3957 1394 Size: 429 2134 3507 Fragments arranged by size: 3507 2134 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4565 4739 65 Size: 1183 3317 174 1396 Fragments arranged by size: 3317 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4714 4901 1700 Size: 110 2904 187 2869 Fragments arranged by size: 2904 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6070 BssHII G'CGCG_C Cuts at: 1925 2146 2148 1925 Size: 221 2 5847 Fragments arranged by size: 5847 221 2 BssSI C'ACGA_G Cuts at: 4130 5142 4130 Size: 1012 5058 BstAPI GCAn_nnn'nTGC Cuts at: 794 2173 2410 794 Size: 1379 237 4454 Fragments arranged by size: 4454 1379 237 BstBI TT'CG_AA Cuts at: 4874 4874 Size: 6070 BstEII G'GTnAC_C Cuts at: 1184 3221 1184 Size: 2037 4033 BstYI r'GATC_y Cuts at: 1972 2226 3400 3767 4152 4169 5621 5707 5719 Size: 254 1174 367 385 17 1452 86 12 Cuts at: 5719 6065 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1174 385 367 346 254 86 17 12 BstZ17I GTA'TAC Cuts at: 2573 2573 Size: 6070 Bsu36I CC'TnA_GG Cuts at: 2385 2385 Size: 6070 BtgI C'CryG_G Cuts at: 513 1065 2136 3208 3351 513 Size: 552 1071 1072 143 3232 Fragments arranged by size: 3232 1072 1071 552 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2233 487 Size: 683 56 1007 4324 Fragments arranged by size: 4324 1007 683 56 BtsI GCAGTG_nn' Cuts at: 3132 3586 4380 4400 3132 Size: 454 794 20 4802 Fragments arranged by size: 4802 794 454 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6070 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6035 DraI TTT'AAA Cuts at: 3374 3374 Size: 6070 DraIII CAC_nnn'GTG Cuts at: 2173 2173 Size: 6070 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5077 809 Size: 507 3761 1802 Fragments arranged by size: 3761 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2437 2622 3211 Size: 54 1425 50 275 18 607 185 589 Cuts at: 3211 3517 4406 6035 8 Size: 306 889 1629 43 Fragments arranged by size: 1629 1425 889 607 589 306 275 185 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3517 1537 Size: 275 1705 4090 Fragments arranged by size: 4090 1705 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2201 2792 3291 4000 1353 Size: 848 591 499 709 3423 Fragments arranged by size: 3423 848 709 591 499 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4657 5031 5177 387 Size: 1064 138 3068 374 146 1280 Fragments arranged by size: 3068 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2211 2211 Size: 6070 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2147 3315 4133 4730 5517 5785 1239 Size: 393 515 1168 818 597 787 268 1524 Fragments arranged by size: 1524 1168 818 787 597 515 393 268 EcoO109I rG'GnC_Cy Cuts at: 2829 3346 2829 Size: 517 5553 EcoRI G'AATT_C Cuts at: 2023 2059 2564 3190 3379 3757 2023 Size: 36 505 626 189 378 4336 Fragments arranged by size: 4336 626 505 378 189 36 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2512 2803 2512 Size: 291 5779 FspI TGC'GCA Cuts at: 4576 4576 Size: 6070 HaeII r_GCGC'y Cuts at: 1678 5217 1678 Size: 3539 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2211 2243 4711 4743 4785 Size: 32 135 32 639 32 2468 32 42 Cuts at: 4785 4817 1373 Size: 32 2626 Fragments arranged by size: 2626 2468 639 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3414 3660 669 Size: 988 281 1476 246 3079 Fragments arranged by size: 3079 1476 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6070 HpaI GTT'AAC Cuts at: 3660 3660 Size: 6070 KpnI G_GTAC'C Cuts at: 3395 3395 Size: 6070 MfeI C'AATT_G Cuts at: 3669 3669 Size: 6070 MluI A'CGCG_T Cuts at: 5946 5946 Size: 6070 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2088 2863 2968 5184 5368 1305 Size: 783 775 105 2216 184 2007 Fragments arranged by size: 2216 2007 783 775 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 2439 3213 10 Size: 54 1425 343 607 774 2867 Fragments arranged by size: 2867 1425 774 607 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4028 4387 4546 518 Size: 648 2862 359 159 2042 Fragments arranged by size: 2862 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3486 4163 5311 5556 1606 Size: 1880 677 1148 245 2120 Fragments arranged by size: 2120 1880 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3208 3351 513 Size: 552 2143 143 3232 Fragments arranged by size: 3232 2143 552 143 NdeI CA'TA_TG Cuts at: 387 387 Size: 6070 NheI G'CTAG_C Cuts at: 1052 3304 1052 Size: 2252 3818 NotI GC'GGCC_GC Cuts at: 3517 3517 Size: 6070 NspI r_CATG'y Cuts at: 1132 1229 2159 2777 3290 3428 4973 1132 Size: 97 930 618 513 138 1545 2229 Fragments arranged by size: 2229 1545 930 618 513 138 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6070 PciI A'CATG_T Cuts at: 2773 4969 2773 Size: 2196 3874 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6070 PmeI GTTT'AAAC Cuts at: 3374 3374 Size: 6070 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4139 4171 5652 5684 4139 Size: 32 1481 32 4525 Fragments arranged by size: 4525 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3346 3346 Size: 6070 PshAI GACnn'nnGTC Cuts at: 1613 2121 1613 Size: 508 5562 PsiI TTA'TAA Cuts at: 3640 3640 Size: 6070 PstI C_TGCA'G Cuts at: 830 3422 830 Size: 2592 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6070 SacI G_AGCT'C Cuts at: 2213 2213 Size: 6070 SalI G'TCGA_C Cuts at: 1936 3412 1936 Size: 1476 4594 SanDI GG'GwC_CC Cuts at: 3346 3346 Size: 6070 SapI GCTCTTCn'nnn_ Cuts at: 2201 2201 Size: 6070 SbfI CC_TGCA'GG Cuts at: 3422 3422 Size: 6070 ScaI AGT'ACT Cuts at: 4318 6030 4318 Size: 1712 4358 SexAI A'CCwGG_T Cuts at: 2586 2586 Size: 6070 SfcI C'TryA_G Cuts at: 826 1045 3418 4553 5234 5425 826 Size: 219 2373 1135 681 191 1471 Fragments arranged by size: 2373 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6070 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6070 SmaI CCC'GGG Cuts at: 1872 3397 1872 Size: 1525 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2347 2869 4174 5075 5337 820 Size: 197 926 404 522 1305 901 262 1553 Fragments arranged by size: 1553 1305 926 901 522 404 262 197 SnaBI TAC'GTA Cuts at: 493 3861 493 Size: 3368 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6070 SphI G_CATG'C Cuts at: 1132 3428 1132 Size: 2296 3774 SspI AAT'ATT Cuts at: 5 52 2278 2461 3994 5 Size: 47 2226 183 1533 2081 Fragments arranged by size: 2226 2081 1533 183 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6070 StyI C'CwwG_G Cuts at: 513 1065 2754 2825 3208 3351 3509 513 Size: 552 1689 71 383 143 158 3074 Fragments arranged by size: 3074 1689 552 383 158 143 71 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2117 4097 4114 4267 4452 4897 325 Size: 1255 537 1980 17 153 185 445 1498 Fragments arranged by size: 1980 1498 1255 537 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2841 4316 6028 Size: 274 80 33 51 1443 864 1475 1712 Cuts at: 6028 96 Size: 138 Fragments arranged by size: 1712 1475 1443 864 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2952 2984 6030 6062 2952 Size: 32 3046 32 2960 Fragments arranged by size: 3046 2960 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6070 XbaI T'CTAG_A Cuts at: 3406 3406 Size: 6070 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2752 3358 2752 Size: 606 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6070 XmaI C'CCGG_G Cuts at: 1870 3395 1870 Size: 1525 4545 XmnI GAAnn'nnTTC Cuts at: 2495 4199 2495 Size: 1704 4366 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2635 3878 276 Size: 53 83 186 936 1101 1243 2468 Fragments arranged by size: 2468 1243 1101 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BfrBI BsiWI BsmBI BstXI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SfiI SfoI SrfI SwaI