Restriction map for
pFN21AB8124
(Circular) (Six-base) MAPSORT of: pFN21AB8124.seq Check: 8160 from: 1 to: 5902
pFN21AB8124 5902 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2574 2574
Size: 5902
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3712 278
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2132 3245 1937
Size: 195 1113 4594
Fragments arranged by size:
4594 1113 195
Acc65I G'GTAC_C
Cuts at: 3223 3223
Size: 5902
AclI AA'CG_TT
Cuts at: 1563 4029 4402 1563
Size: 2466 373 3063
Fragments arranged by size:
3063 2466 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2564 2727 3965 5349 5617 1632
Size: 932 163 1238 1384 268 1917
Fragments arranged by size:
1917 1384 1238 932 268 163
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5705
AflIII A'CryG_T
Cuts at: 1203 4801 5778 1203
Size: 3598 977 1327
Fragments arranged by size:
3598 1327 977
AgeI A'CCGG_T
Cuts at: 2480 4733 2480
Size: 2253 3649
AhdI GACnn_n'nnGTC
Cuts at: 4631 4631
Size: 5902
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5902
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5902
AlwNI CAG_nnn'CTG
Cuts at: 2542 3645 5217 2542
Size: 1103 1572 3227
Fragments arranged by size:
3227 1572 1103
ApaI G_GGCC'C
Cuts at: 2046 2312 2356 2046
Size: 266 44 5592
Fragments arranged by size:
5592 266 44
ApaLI G'TGCA_C
Cuts at: 3959 5115 3959
Size: 1156 4746
ApoI r'AATT_y
Cuts at: 1493 3155 3188 3211 3437 3589 5698 1493
Size: 1662 33 23 226 152 2109 1697
Fragments arranged by size:
2109 1697 1662 226 152 33 23
AseI AT'TA_AT
Cuts at: 160 1032 4456 160
Size: 872 3424 1606
Fragments arranged by size:
3424 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
AvaI C'yCGr_G
Cuts at: 1870 1943 2180 2204 2227 2348 2778 3227 1870
Size: 73 237 24 23 121 430 449 4545
Fragments arranged by size:
4545 449 430 237 121 73 24 23
BamHI G'GATC_C
Cuts at: 3232 3599 3232
Size: 367 5535
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2156 2366 3223 4678 618
Size: 325 217 627 369 210 857 1455 1842
Fragments arranged by size:
1842 1455 857 627 369 325 217 210
BanII G_rGCy'C
Cuts at: 1391 2046 2181 2312 2356 2516 2615 2813 3221
Size: 655 135 131 44 160 99 198 408
Cuts at: 3221 1391
Size: 4072
Fragments arranged by size:
4072 655 408 198 160 135 131 99
44
BbeI G_GCGC'C
Cuts at: 2370 2370
Size: 5902
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2877 928
Size: 971 978 3953
Fragments arranged by size:
3953 978 971
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4093 4127 1903
Size: 34 2156 34 3678
Fragments arranged by size:
3678 2156 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3124 3794 5004 3124
Size: 670 1210 4022
Fragments arranged by size:
4022 1210 670
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5902
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2321 2389 4513
Size: 107 122 71 631 605 649 68 2124
Cuts at: 4513 136
Size: 1525
Fragments arranged by size:
2124 1525 649 631 605 122 107 71
68
BglII A'GATC_T
Cuts at: 5897 5897
Size: 5902
BlpI GC'TnA_GC
Cuts at: 3319 3319
Size: 5902
Bme1580I G_kGCm'C
Cuts at: 1790 2046 2312 2356 3963 5119 1790
Size: 256 266 44 1607 1156 2573
Fragments arranged by size:
2573 1607 1156 266 256 44
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5902
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3633 4591 442
Size: 416 53 83 399 24 2216 958 1753
Fragments arranged by size:
2216 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5902
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2530 2890 2530
Size: 360 5542
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2306 2312 2679 3053 4562 1239
Size: 1067 6 367 374 1509 2579
Fragments arranged by size:
2579 1509 1067 374 367 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2543 2615 4027 4892 5190 2543
Size: 72 1412 865 298 3255
Fragments arranged by size:
3255 1412 865 298 72
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4565 882
Size: 617 3066 2219
Fragments arranged by size:
3066 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2101 2446 3693 493
Size: 711 897 345 1247 2702
Fragments arranged by size:
2702 1247 897 711 345
BsaBI GATnn'nnATC
Cuts at: 2850 3078 5896 2850
Size: 228 2818 2856
Fragments arranged by size:
2856 2818 228
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2367 3709 4091 4726
Size: 53 83 186 936 834 1342 382 635
Cuts at: 4726 275
Size: 1451
Fragments arranged by size:
1451 1342 936 834 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2480 4335 4733 5007 5154 5565 2480
Size: 1855 398 274 147 411 2817
Fragments arranged by size:
2817 1855 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2069 2099 2570 2600 1178
Size: 30 165 30 666 30 471 30 4480
Fragments arranged by size:
4480 666 471 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2608 2660 2804 2909 1186
Size: 1422 52 144 105 4179
Fragments arranged by size:
4179 1422 144 105 52
BseYI C'CCAG_C
Cuts at: 2108 2199 2305 2468 2593 5105 5601 2108
Size: 91 106 163 125 2512 496 2409
Fragments arranged by size:
2512 2409 496 163 125 106 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 2192 1163
Size: 573 393 63 4873
Fragments arranged by size:
4873 573 393 63
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3352 4113 5141 1540
Size: 275 186 1351 761 1028 2301
Fragments arranged by size:
2301 1351 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2516 3166 3963 4048 5119 2516
Size: 650 797 85 1071 3299
Fragments arranged by size:
3299 1071 797 650 85
BsmI GAATG_Cn'
Cuts at: 3413 3506 3413
Size: 93 5809
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2046 2181 2312 2356 2516 2615 2813
Size: 399 256 135 131 44 160 99 198
Cuts at: 2813 3166 3221 3963 4048 5119 1391
Size: 353 55 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 353 256 198 160
135 131 99 85 55 44
BspEI T'CCGG_A
Cuts at: 5565 5565
Size: 5902
BspHI T'CATG_A
Cuts at: 3791 5521 3791
Size: 1730 4172
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2574 2598 3257 844
Size: 1730 24 659 3489
Fragments arranged by size:
3489 1730 659 24
BsrBI CCG'CTC
Cuts at: 1394 1823 2463 3789 1394
Size: 429 640 1326 3507
Fragments arranged by size:
3507 1326 640 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4397 4571 65
Size: 1183 3149 174 1396
Fragments arranged by size:
3149 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2330 2480 2605 4546 4733 1700
Size: 110 520 150 125 1941 187 2869
Fragments arranged by size:
2869 1941 520 187 150 125 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5902
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5902
BssSI C'ACGA_G
Cuts at: 2530 3962 4974 2530
Size: 1432 1012 3458
Fragments arranged by size:
3458 1432 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2163 794
Size: 1369 4533
BstBI TT'CG_AA
Cuts at: 3193 4706 3193
Size: 1513 4389
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5902
BstYI r'GATC_y
Cuts at: 1972 2986 3232 3599 3984 4001 5453 5539 5551
Size: 1014 246 367 385 17 1452 86 12
Cuts at: 5551 5897 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1014 385 367 346 246 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2865 2865
Size: 5902
BtgI C'CryG_G
Cuts at: 513 1065 2004 2370 2661 513
Size: 552 939 366 291 3754
Fragments arranged by size:
3754 939 552 366 291
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2030 2516 487
Size: 683 56 804 486 3873
Fragments arranged by size:
3873 804 683 486 56
BtsI GCAGTG_nn'
Cuts at: 3418 4212 4232 3418
Size: 794 20 5088
Fragments arranged by size:
5088 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5902
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5867
DraI TTT'AAA
Cuts at: 3206 3206
Size: 5902
DraIII CAC_nnn'GTG
Cuts at: 2163 2163
Size: 5902
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4909 809
Size: 507 3593 1802
Fragments arranged by size:
3593 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2115 2217 2358
Size: 54 1425 50 275 18 285 102 141
Cuts at: 2358 3349 4238 5867 8
Size: 991 889 1629 43
Fragments arranged by size:
1629 1425 991 889 285 275 141 102
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3349 1537
Size: 275 1537 4090
Fragments arranged by size:
4090 1537 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2504 2706 3832 1353
Size: 1151 202 1126 3423
Fragments arranged by size:
3423 1151 1126 202
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2339 2416 2657 3034 4489
Size: 1064 138 435 315 77 241 377 1455
Cuts at: 4489 4863 5009 387
Size: 374 146 1280
Fragments arranged by size:
1455 1280 1064 435 377 374 315 241
146 138 77
EcoICRI GAG'CTC
Cuts at: 2514 2514
Size: 5902
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2306 2312 2564 2679 2727 3053 3965
Size: 393 674 6 252 115 48 326 912
Cuts at: 3965 4562 5349 5617 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 912 787 674 597 393 326 268
252 115 48 6
EcoNI CCTnn'n_nnAGG
Cuts at: 2360 2360
Size: 5902
EcoO109I rG'GnC_Cy
Cuts at: 2309 2352 2353 2650 2309
Size: 43 1 297 5561
Fragments arranged by size:
5561 297 43 1
EcoRI G'AATT_C
Cuts at: 3211 3589 3211
Size: 378 5524
EcoRV GAT'ATC
Cuts at: 3080 3080
Size: 5902
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2998 2998
Size: 5902
FspI TGC'GCA
Cuts at: 4408 4408
Size: 5902
HaeII r_GCGC'y
Cuts at: 1678 2216 2370 5049 1678
Size: 538 154 2679 2531
Fragments arranged by size:
2679 2531 538 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2069 2101 2225 2257 2318
Size: 32 135 32 497 32 124 32 61
Cuts at: 2318 2350 4543 4575 4617 4649 1373
Size: 32 2193 32 42 32 2626
Fragments arranged by size:
2626 2193 497 135 124 61 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2133 3246 3492 669
Size: 988 281 195 1113 246 3079
Fragments arranged by size:
3079 1113 988 281 246 195
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5902
HpaI GTT'AAC
Cuts at: 3492 3492
Size: 5902
KasI G'GCGC_C
Cuts at: 2366 2366
Size: 5902
KpnI G_GTAC'C
Cuts at: 3227 3227
Size: 5902
MfeI C'AATT_G
Cuts at: 3501 3501
Size: 5902
MluI A'CGCG_T
Cuts at: 5778 5778
Size: 5902
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5016 5200 1305
Size: 3711 184 2007
Fragments arranged by size:
3711 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4080
Fragments arranged by size:
4080 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3860 4219 4378 518
Size: 648 2694 359 159 2042
Fragments arranged by size:
2694 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2372 2389 2663 3318 3995 5143 5388 1606
Size: 766 17 274 655 677 1148 245 2120
Fragments arranged by size:
2120 1148 766 677 655 274 245 17
NarI GG'CG_CC
Cuts at: 2367 2367
Size: 5902
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4411
Fragments arranged by size:
4411 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5902
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5902
NotI GC'GGCC_GC
Cuts at: 3349 3349
Size: 5902
NspI r_CATG'y
Cuts at: 1132 1229 3260 4805 1132
Size: 97 2031 1545 2229
Fragments arranged by size:
2229 2031 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5902
PciI A'CATG_T
Cuts at: 4801 4801
Size: 5902
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5902
PfoI T'CCnGG_A
Cuts at: 2256 2733 2256
Size: 477 5425
PmeI GTTT'AAAC
Cuts at: 3206 3206
Size: 5902
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3971 4003 5484 5516 3971
Size: 32 1481 32 4357
Fragments arranged by size:
4357 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2650 2650
Size: 5902
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5902
PsiI TTA'TAA
Cuts at: 3472 3472
Size: 5902
PspOMI G'GGCC_C
Cuts at: 2042 2308 2352 2042
Size: 266 44 5592
Fragments arranged by size:
5592 266 44
PstI C_TGCA'G
Cuts at: 830 3254 830
Size: 2424 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5902
SacI G_AGCT'C
Cuts at: 2516 2516
Size: 5902
SacII CC_GC'GG
Cuts at: 2373 2664 2373
Size: 291 5611
SalI G'TCGA_C
Cuts at: 1936 2131 3244 1936
Size: 195 1113 4594
Fragments arranged by size:
4594 1113 195
SapI GCTCTTCn'nnn_
Cuts at: 2504 2504
Size: 5902
SbfI CC_TGCA'GG
Cuts at: 3254 3254
Size: 5902
ScaI AGT'ACT
Cuts at: 4150 5862 4150
Size: 1712 4190
SfcI C'TryA_G
Cuts at: 826 1045 3250 4385 5066 5257 826
Size: 219 2205 1135 681 191 1471
Fragments arranged by size:
2205 1471 1135 681 219 191
SfoI GGC'GCC
Cuts at: 2368 2368
Size: 5902
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5902
SgrAI Cr'CCGG_yG
Cuts at: 1700 2605 1700
Size: 905 4997
SmaI CCC'GGG
Cuts at: 1872 2182 2206 2350 2780 3229 1872
Size: 310 24 144 430 449 4545
Fragments arranged by size:
4545 449 430 310 144 24
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2558 2630 4006 4907 5169 820
Size: 197 926 615 72 1376 901 262 1553
Fragments arranged by size:
1553 1376 926 901 615 262 197 72
SnaBI TAC'GTA
Cuts at: 493 3693 493
Size: 3200 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5902
SphI G_CATG'C
Cuts at: 1132 3260 1132
Size: 2128 3774
SspI AAT'ATT
Cuts at: 5 52 3826 5
Size: 47 3774 2081
Fragments arranged by size:
3774 2081 47
StuI AGG'CCT
Cuts at: 1517 2225 2297 3008 1517
Size: 708 72 711 4411
Fragments arranged by size:
4411 711 708 72
StyI C'CwwG_G
Cuts at: 513 1065 2004 3341 513
Size: 552 939 1337 3074
Fragments arranged by size:
3074 1337 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2063 2872 3929 3946 4099 4284 4729
Size: 1255 483 809 1057 17 153 185 445
Cuts at: 4729 325
Size: 1498
Fragments arranged by size:
1498 1255 1057 809 483 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2250 4148 5860
Size: 274 80 33 51 1443 273 1898 1712
Cuts at: 5860 96
Size: 138
Fragments arranged by size:
1898 1712 1443 274 273 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2994 3026 5862 5894 2994
Size: 32 2836 32 3002
Fragments arranged by size:
3002 2836 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5902
XbaI T'CTAG_A
Cuts at: 3238 3238
Size: 5902
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3142 3142
Size: 5902
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5902
XmaI C'CCGG_G
Cuts at: 1870 2180 2204 2348 2778 3227 1870
Size: 310 24 144 430 449 4545
Fragments arranged by size:
4545 449 430 310 144 24
XmnI GAAnn'nnTTC
Cuts at: 4031 4031
Size: 5902
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3710 276
Size: 53 83 186 936 2176 2468
Fragments arranged by size:
2468 2176 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MmeI MscI MslI
MspA1I NarI NcoI NdeI NheI NotI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI SacI SacII SalI SapI SbfI
ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I
BsiWI BsmBI BstXI BstZ17I FseI FspAI NaeI NgoMIV
NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII
SanDI SexAI SfiI SrfI SwaI