(Circular) (Six-base) MAPSORT of: pFN21AB8123.seq Check: 3700 from: 1 to: 6256 pFN21AB8123 6256 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2440 2440 Size: 6256 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2585 4066 278 Size: 53 83 186 936 1049 1481 2468 Fragments arranged by size: 2468 1481 1049 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2353 2859 3548 3599 1937 Size: 416 506 689 51 4594 Fragments arranged by size: 4594 689 506 416 51 Acc65I G'GTAC_C Cuts at: 3577 3577 Size: 6256 AclI AA'CG_TT Cuts at: 1563 4383 4756 1563 Size: 2820 373 3063 Fragments arranged by size: 3063 2820 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2772 3315 4319 5703 5971 1632 Size: 1140 543 1004 1384 268 1917 Fragments arranged by size: 1917 1384 1140 1004 543 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6059 AflIII A'CryG_T Cuts at: 1203 5155 6132 1203 Size: 3952 977 1327 Fragments arranged by size: 3952 1327 977 AgeI A'CCGG_T Cuts at: 5087 5087 Size: 6256 AhdI GACnn_n'nnGTC Cuts at: 4985 4985 Size: 6256 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6256 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6256 AlwNI CAG_nnn'CTG Cuts at: 3999 5571 3999 Size: 1572 4684 ApaLI G'TGCA_C Cuts at: 4313 5469 4313 Size: 1156 5100 ApoI r'AATT_y Cuts at: 1493 2043 3308 3356 3407 3565 3791 3943 6052 Size: 550 1265 48 51 158 226 152 2109 Cuts at: 6052 1493 Size: 1697 Fragments arranged by size: 2109 1697 1265 550 226 158 152 51 48 AseI AT'TA_AT Cuts at: 160 1032 2337 4810 160 Size: 872 1305 2473 1606 Fragments arranged by size: 2473 1606 1305 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 AvaI C'yCGr_G Cuts at: 1870 1943 2880 3581 1870 Size: 73 937 701 4545 Fragments arranged by size: 4545 937 701 73 BamHI G'GATC_C Cuts at: 3586 3953 3586 Size: 367 5889 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2495 2962 3577 5032 618 Size: 325 217 627 708 467 615 1455 1842 Fragments arranged by size: 1842 1455 708 627 615 467 325 217 BanII G_rGCy'C Cuts at: 1391 2942 3575 1391 Size: 1551 633 4072 Fragments arranged by size: 4072 1551 633 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2194 2760 928 Size: 971 295 566 4424 Fragments arranged by size: 4424 971 566 295 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4447 4481 1903 Size: 34 2510 34 3678 Fragments arranged by size: 3678 2510 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3183 4148 5358 3183 Size: 965 1210 4081 Fragments arranged by size: 4081 1210 965 BclI T'GATC_A Cuts at: 1548 1548 Size: 6256 BfrBI ATG'CAT Cuts at: 3209 3209 Size: 6256 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4867 136 Size: 107 122 71 631 605 3195 1525 Fragments arranged by size: 3195 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6251 6251 Size: 6256 BlpI GC'TnA_GC Cuts at: 3673 3673 Size: 6256 Bme1580I G_kGCm'C Cuts at: 1790 2667 4317 5473 1790 Size: 877 1650 1156 2573 Fragments arranged by size: 2573 1650 1156 877 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6256 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2736 3136 3987 Size: 416 53 83 399 24 1319 400 851 Cuts at: 3987 4945 442 Size: 958 1753 Fragments arranged by size: 1753 1319 958 851 416 400 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6256 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2409 2571 2882 2952 4916 1239 Size: 1170 162 311 70 1964 2579 Fragments arranged by size: 2579 1964 1170 311 162 70 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2614 3023 4381 5246 5544 2614 Size: 409 1358 865 298 3326 Fragments arranged by size: 3326 1358 865 409 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4919 882 Size: 617 3420 2219 Fragments arranged by size: 3420 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4047 493 Size: 711 2843 2702 Fragments arranged by size: 2843 2702 711 BsaBI GATnn'nnATC Cuts at: 6250 6250 Size: 6256 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2582 4063 4445 5080 Size: 53 83 186 936 1049 1481 382 635 Cuts at: 5080 275 Size: 1451 Fragments arranged by size: 1481 1451 1049 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4689 5087 5361 5508 5919 4689 Size: 398 274 147 411 5026 Fragments arranged by size: 5026 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2382 2412 2488 2518 3092 Size: 30 165 30 979 30 76 30 574 Cuts at: 3092 3122 1178 Size: 30 4312 Fragments arranged by size: 4312 979 574 165 76 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2042 2241 2491 2496 1186 Size: 856 199 250 5 4946 Fragments arranged by size: 4946 856 250 199 5 BseYI C'CCAG_C Cuts at: 5459 5955 5459 Size: 496 5760 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 2707 3104 1163 Size: 573 339 632 397 4315 Fragments arranged by size: 4315 632 573 397 339 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3706 4467 5495 1540 Size: 275 186 1705 761 1028 2301 Fragments arranged by size: 2301 1705 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2431 2476 4317 4402 5473 2431 Size: 45 1841 85 1071 3214 Fragments arranged by size: 3214 1841 1071 85 45 BsmI GAATG_Cn' Cuts at: 2389 2695 3438 3767 3860 2389 Size: 306 743 329 93 4785 Fragments arranged by size: 4785 743 329 306 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2589 2589 Size: 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2431 2476 2667 2942 3575 4317 4402 Size: 399 641 45 191 275 633 742 85 Cuts at: 4402 5473 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 641 633 399 275 191 85 45 BspEI T'CCGG_A Cuts at: 5919 5919 Size: 6256 BspHI T'CATG_A Cuts at: 4145 5875 4145 Size: 1730 4526 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2440 3060 3611 844 Size: 1596 620 551 3489 Fragments arranged by size: 3489 1596 620 551 BsrBI CCG'CTC Cuts at: 1394 1823 2282 4143 1394 Size: 429 459 1861 3507 Fragments arranged by size: 3507 1861 459 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2703 4751 4925 65 Size: 1183 1455 2048 174 1396 Fragments arranged by size: 2048 1455 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4900 5087 1700 Size: 110 3090 187 2869 Fragments arranged by size: 3090 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6256 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6256 BssSI C'ACGA_G Cuts at: 3532 4316 5328 3532 Size: 784 1012 4460 Fragments arranged by size: 4460 1012 784 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6256 BstBI TT'CG_AA Cuts at: 5060 5060 Size: 6256 BstEII G'GTnAC_C Cuts at: 1184 2063 2176 1184 Size: 879 113 5264 Fragments arranged by size: 5264 879 113 BstXI CCAn_nnnn'nTGG Cuts at: 2492 2492 Size: 6256 BstYI r'GATC_y Cuts at: 1972 3033 3586 3953 4338 4355 5807 5893 5905 Size: 1061 553 367 385 17 1452 86 12 Cuts at: 5905 6251 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1061 553 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3549 3549 Size: 6256 Bsu36I CC'TnA_GG Cuts at: 2933 2933 Size: 6256 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2623 487 Size: 683 56 1397 4120 Fragments arranged by size: 4120 1397 683 56 BtsI GCAGTG_nn' Cuts at: 2497 3433 3772 4566 4586 2497 Size: 936 339 794 20 4167 Fragments arranged by size: 4167 936 794 339 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6256 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6221 DraI TTT'AAA Cuts at: 2421 3560 2421 Size: 1139 5117 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5263 809 Size: 507 3947 1802 Fragments arranged by size: 3947 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2576 3703 4592 Size: 54 1425 50 275 18 746 1127 889 Cuts at: 4592 6221 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1127 889 746 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3703 1537 Size: 275 1891 4090 Fragments arranged by size: 4090 1891 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4186 1353 Size: 2833 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4843 5217 5363 387 Size: 1064 138 3254 374 146 1280 Fragments arranged by size: 3254 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2409 2571 2772 2882 2952 3315 4319 Size: 393 777 162 201 110 70 363 1004 Cuts at: 4319 4916 5703 5971 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1004 787 777 597 393 363 268 201 162 110 70 EcoO109I rG'GnC_Cy Cuts at: 3103 3103 Size: 6256 EcoRI G'AATT_C Cuts at: 3565 3943 3565 Size: 378 5878 FspI TGC'GCA Cuts at: 4762 4762 Size: 6256 HaeII r_GCGC'y Cuts at: 1678 5403 1678 Size: 3725 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2546 2578 4897 4929 4971 Size: 32 135 32 974 32 2319 32 42 Cuts at: 4971 5003 1373 Size: 32 2626 Fragments arranged by size: 2626 2319 974 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2764 3600 3846 669 Size: 988 281 826 836 246 3079 Fragments arranged by size: 3079 988 836 826 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6256 HpaI GTT'AAC Cuts at: 3846 3846 Size: 6256 KpnI G_GTAC'C Cuts at: 3581 3581 Size: 6256 MfeI C'AATT_G Cuts at: 3243 3855 3243 Size: 612 5644 MluI A'CGCG_T Cuts at: 6132 6132 Size: 6256 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3384 5370 5554 1305 Size: 2079 1986 184 2007 Fragments arranged by size: 2079 2007 1986 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4434 Fragments arranged by size: 4434 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2111 2339 2563 2708 4214 4573 4732 Size: 648 945 228 224 145 1506 359 159 Cuts at: 4732 518 Size: 2042 Fragments arranged by size: 2042 1506 945 648 359 228 224 159 145 MspA1I CmG'CkG Cuts at: 1606 3672 4349 5497 5742 1606 Size: 2066 677 1148 245 2120 Fragments arranged by size: 2120 2066 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4765 Fragments arranged by size: 4765 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6256 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6256 NotI GC'GGCC_GC Cuts at: 3703 3703 Size: 6256 NsiI A_TGCA'T Cuts at: 3211 3211 Size: 6256 NspI r_CATG'y Cuts at: 1132 1229 2472 3213 3614 5159 1132 Size: 97 1243 741 401 1545 2229 Fragments arranged by size: 2229 1545 1243 741 401 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6256 PciI A'CATG_T Cuts at: 5155 5155 Size: 6256 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6256 PfoI T'CCnGG_A Cuts at: 2118 2898 2118 Size: 780 5476 PmeI GTTT'AAAC Cuts at: 3560 3560 Size: 6256 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4325 4357 5838 5870 4325 Size: 32 1481 32 4711 Fragments arranged by size: 4711 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3103 3103 Size: 6256 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6256 PsiI TTA'TAA Cuts at: 3826 3826 Size: 6256 PstI C_TGCA'G Cuts at: 830 2058 3608 830 Size: 1228 1550 3478 Fragments arranged by size: 3478 1550 1228 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6256 SalI G'TCGA_C Cuts at: 1936 3598 1936 Size: 1662 4594 SbfI CC_TGCA'GG Cuts at: 3608 3608 Size: 6256 ScaI AGT'ACT Cuts at: 4504 6216 4504 Size: 1712 4544 SfcI C'TryA_G Cuts at: 826 1045 2054 2321 3604 4739 5420 5611 826 Size: 219 1009 267 1283 1135 681 191 1471 Fragments arranged by size: 1471 1283 1135 1009 681 267 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6256 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6256 SmaI CCC'GGG Cuts at: 1872 2882 3583 1872 Size: 1010 701 4545 Fragments arranged by size: 4545 1010 701 SmlI C'TyrA_G Cuts at: 820 1017 1943 2593 3038 4360 5261 5523 820 Size: 197 926 650 445 1322 901 262 1553 Fragments arranged by size: 1553 1322 926 901 650 445 262 197 SnaBI TAC'GTA Cuts at: 493 4047 493 Size: 3554 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6256 SphI G_CATG'C Cuts at: 1132 3614 1132 Size: 2482 3774 SspI AAT'ATT Cuts at: 5 52 2815 4180 5 Size: 47 2763 1365 2081 Fragments arranged by size: 2763 2081 1365 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6256 StyI C'CwwG_G Cuts at: 513 1065 2004 2268 3459 3695 513 Size: 552 939 264 1191 236 3074 Fragments arranged by size: 3074 1191 939 552 264 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3075 4283 4300 4453 4638 5083 325 Size: 1255 1495 1208 17 153 185 445 1498 Fragments arranged by size: 1498 1495 1255 1208 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2910 3108 4502 Size: 274 80 33 51 1443 933 198 1394 Cuts at: 4502 6214 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1394 933 274 198 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2382 2414 2421 2453 2488 2520 6216 6248 2382 Size: 32 7 32 35 32 3696 32 2390 Fragments arranged by size: 3696 2390 35 32 32 32 32 7 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6256 XbaI T'CTAG_A Cuts at: 3175 3592 3175 Size: 417 5839 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2595 2976 2595 Size: 381 5875 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6256 XmaI C'CCGG_G Cuts at: 1870 2880 3581 1870 Size: 1010 701 4545 Fragments arranged by size: 4545 1010 701 XmnI GAAnn'nnTTC Cuts at: 2367 3005 4385 2367 Size: 638 1380 4238 Fragments arranged by size: 4238 1380 638 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2583 4064 276 Size: 53 83 186 936 1049 1481 2468 Fragments arranged by size: 2468 1481 1049 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI