Restriction map for
pFN21AB8122
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccIII AcsI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AviII AvrII BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsiWI
BsmBI BsmFI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
FauI FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MluI MroI MseI MslI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI PvuI RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tru9I Tsp509I Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccII 181 CGCG CG^CG
AciI 81 GCGG CCGC(-3/-1)
AclI 356 AACGTT AA^CGTT
AlwI 189 GATCC GGATC(4/5)
BalI 348 TGGCCA TGG^CCA
BanI 501 GGTGCC G^G[CT][GA]CC
BcnI 318 CCGGG CC^[GC]GG
BsaI 270 GGTCTC GGTCTC(1/5)
BsgI 286 GTGCAG GTGCAG(16/14)
BsiEI 44 CGACCG CG[GA][CT]^CG
BsrDI 237 CATTGC GCAATG(2/0)
BsrFI 228 GCCGGT [GA]^CCGG[CT]
BstEII 231 GGTCACC G^GT[ACGT]ACC
BstPI 231 GGTCACC G^GT[ACGT]ACC
BstUI 181 CGCG CG^CG
BstYI 188 AGATCC [GA]^GATC[CT]
CfoI 14 GCGC GCG^C
Cfr10I 228 GCCGGT [GA]^CCGG[CT]
DpnII 189 GATC ^GATC
DraII 330 AGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 348 TGGCCA [CT]^GGCC[GA]
EcoO109I 330 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 231 GGTCACC G^GT[ACGT]ACC
EcoT22I 175 ATGCAT ATGCA^T
HaeII 13 AGCGCC [GA]GCGC^[CT]
HhaI 14 GCGC GCG^C
HinP1I 14 GCGC G^CGC
HphI 233 TCACC GGTGA(8/7)
MaeII 357 ACGT A^CGT
MboI 189 GATC ^GATC
MflI 188 AGATCC [GA]^GATC[CT]
MluNI 348 TGGCCA TGG^CCA
MscI 348 TGGCCA TGG^CCA
MspA1I 103 CAGCTG C[AC]G^C[GT]G
MvnI 181 CGCG CG^CG
NciI 318 CCGGG CC^[GC]GG
NdeII 189 GATC ^GATC
NsiI 175 ATGCAT ATGCA^T
PpuMI 330 AGGACCC [GA]G^G[AT]CC[CT]
Psp1406I 356 AACGTT AA^CGTT
PstI 123 CTGCAG CTGCA^G
PvuII 103 CAGCTG CAG^CTG
SacI 64 GAGCTC GAGCT^C
Sau3AI 189 GATC ^GATC
SfcI 123 CTGCAG C^T[GA][CT]AG
StuI 251 AGGCCT AGG^CCT
XhoII 188 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AspHI 49 GTGCTC G[AT]GC[AT]^C
64 GAGCTC
AvaII 89 GGACC G^G[AT]CC
331 GGACC
BanII 64 GAGCTC G[GA]GC[CT]^C
585 GAGCCC
BsiHKAI 49 GTGCTC G[AT]GC[AT]^C
64 GAGCTC
BsmAI 578 GAGAC GTCTC(1/5)
271 GTCTC
BsrI 17 CCAGT ACTGG(1/-1)
301 CCAGT
BstF5I 441 GGATG GGATG(2/0)
447 GGATG
Cfr13I 89 GGACC G^G[ACGT]CC
331 GGACC
DsaI 266 CCATGG C^C[GA][CT]GG
275 CCATGG
EcoT14I 266 CCATGG C^C[AT][AT]GG
275 CCATGG
FokI 441 GGATG GGATG(9/13)
447 GGATG
HapII 229 CCGG C^CGG
318 CCGG
HinfI 3 GAATC G^A[ACGT]TC
632 GATTC
HpaII 229 CCGG C^CGG
318 CCGG
MspI 229 CCGG C^CGG
318 CCGG
NcoI 266 CCATGG C^CATGG
275 CCATGG
NlaIII 267 CATG CATG^
276 CATG
NlaIV 331 GGACCC GG[ACGT]^[ACGT]CC
501 GGTGCC
Sau96I 89 GGACC G^G[ACGT]CC
331 GGACC
StyI 266 CCATGG C^C[AT][AT]GG
275 CCATGG
TfiI 3 GAATC G^A[AT]TC
632 GATTC
Tsp45I 40 GTGAC ^GT[GC]AC
232 GTCAC
TspRI 38 CAGTG CA[GC]TG[ACGT][ACGT]^
345 CAGTG
XcmI 266 CCATGGTCTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
312 CCAGCTCCGGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I DdeI EarI EcoRII
Fnu4HI HaeIII ItaI Ksp632I MaeIII
MboII MnlI MvaI MwoI ScrFI
SfaNI TseI