Restriction map for
pFN21AB8119
##################################
# NO CUT ENZYMES ( 138 enzymes) #
##################################
Acc65I AccIII AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BfaI BfrI BglII
BlnI BpuAI BsaAI BsaBI BsaI
BsaWI BseAI BseRI BsgI BsiHKAI
BsiWI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrGI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eam1105I EarI
Eco47III Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FokI FspI
HindIII HpaI KasI KpnI Ksp632I
MaeI MamI MfeI MflI MluI
MroI MseI MslI MunI NarI
NcoI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SapI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
SspBI SwaI Tru9I Tth111I XbaI
XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 259 GACGTC GACGT^C
AccI 54 GTCGAC GT^[AC][GT]AC
AluI 332 AGCT AG^CT
AlwI 421 GATCC GGATC(4/5)
AlwNI 458 CAGGAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 280 GGGCCC GGGCC^C
AvaI 285 CCCGGG C^[CT]CG[GA]G
BalI 212 TGGCCA TGG^CCA
BcgI 499 GCAAAGCATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 43 GCCCTGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 270 GCTCAGC GC^T[ACGT]AGC
BpmI 229 CTGGAG CTGGAG(16/14)
Bpu1102I 270 GCTCAGC GC^T[ACGT]AGC
BsmAI 193 GTCTC GTCTC(1/5)
BsmBI 192 CGTCTC CGTCTC(1/5)
BsmI 504 GCATTC GAATGC(1/-1)
BsoBI 285 CCCGGG C^[CT]CG[GA]G
BspMI 347 ACCTGC ACCTGC(4/8)
BsrFI 245 GCCGGC [GA]^CCGG[CT]
BssSI 73 CTCGTG CACGAG(-5/-1)
BstXI 377 CCATGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 270 GCTCAGC GC^T[ACGT]AGC
Cfr10I 245 GCCGGC [GA]^CCGG[CT]
DpnII 421 GATC ^GATC
EcoT14I 470 CCAAGG C^C[AT][AT]GG
FseI 244 GGCCGGCC GGCCGG^CC
HincII 54 GTCGAC GT[CT]^[GA]AC
HindII 54 GTCGAC GT[CT]^[GA]AC
HinfI 357 GAATC G^A[ACGT]TC
HphI 474 GGTGA GGTGA(8/7)
KspI 15 CCGCGG CCGC^GG
MboI 421 GATC ^GATC
MboII 634 GAAGA GAAGA(8/7)
MluNI 212 TGGCCA TGG^CCA
MscI 212 TGGCCA TGG^CCA
NaeI 245 GCCGGC GCC^GGC
NdeI 362 CATATG CA^TATG
NdeII 421 GATC ^GATC
NgoMIV 245 GCCGGC G^CCGGC
PflMI 584 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 593 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 186 GACGCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 15 CCGCGG CCGC^GG
SalI 54 GTCGAC G^TCGAC
Sau3AI 421 GATC ^GATC
SbfI 348 CCTGCAGG CCTGCA^GG
SmaI 285 CCCGGG CCC^GGG
Sse8387I 348 CCTGCAGG CCTGCA^GG
SspI 638 AATATT AAT^ATT
StuI 125 AGGCCT AGG^CCT
StyI 470 CCAAGG C^C[AT][AT]GG
TfiI 357 GAATC G^A[AT]TC
Tsp509I 556 AATT ^AATT
Van91I 584 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 218 CCATCTCCACGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 285 CCCGGG C^CCGGG
XmaI 285 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 51 GGCGTC G[GA]^CG[CT]C
259 GACGTC
BanII 280 GGGCCC G[GA]GC[CT]^C
321 GAGCCC
BsaHI 51 GGCGTC G[GA]^CG[CT]C
259 GACGTC
BsiEI 99 CGGCCG CG[GA][CT]^CG
243 CGGCCG
BsrI 116 ACTGG ACTGG(1/-1)
200 ACTGG
BstNI 539 CCTGG CC^[AT]GG
597 CCTGG
DraII 280 GGGCCCC [GA]G^G[ACGT]CC[CT]
593 GGGTCCT
DsaI 15 CCGCGG C^C[GA][CT]GG
108 CCACGG
EagI 99 CGGCCG C^GGCCG
243 CGGCCG
EclXI 99 CGGCCG C^GGCCG
243 CGGCCG
Eco52I 99 CGGCCG C^GGCCG
243 CGGCCG
EcoO109I 280 GGGCCCC [GA]G^G[ACGT]CC[CT]
593 GGGTCCT
EcoRII 539 CCTGG ^CC[AT]GG
597 CCTGG
HaeII 66 GGCGCT [GA]GCGC^[CT]
205 GGCGCT
Hin1I 51 GGCGTC G[GA]^CG[CT]C
259 GACGTC
MaeII 191 ACGT A^CGT
260 ACGT
MvaI 539 CCTGG CC^[AT]GG
597 CCTGG
NlaIII 378 CATG CATG^
412 CATG
PstI 328 CTGCAG CTGCA^G
349 CTGCAG
SfcI 328 CTGCAG C^T[GA][CT]AG
349 CTGCAG
TaqI 55 TCGA T^CGA
519 TCGA
TspRI 32 CACTG CA[GC]TG[ACGT][ACGT]^
371 CACTG
TthHB8I 55 TCGA T^CGA
519 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AccII AciI AvaII BbvI BcnI
BmyI BsaJI BsiYI BslI BsmFI
Bsp1286I BssHII BssKI BstUI Cac8I
CfoI Cfr13I DdeI EaeI FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HpaII ItaI MaeIII MnlI
MspA1I MspI MvnI MwoI NciI
NlaIV Sau96I ScrFI TseI Tsp45I