(Circular) (Six-base) MAPSORT of: pFN21AB8119.seq Check: 4411 from: 1 to: 5365 pFN21AB8119 5365 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2268 3175 278 Size: 53 83 186 936 732 907 2468 Fragments arranged by size: 2468 936 907 732 186 83 53 AccI GT'mk_AC Cuts at: 1937 2060 2708 1937 Size: 123 648 4594 Fragments arranged by size: 4594 648 123 Acc65I G'GTAC_C Cuts at: 2686 2686 Size: 5365 AclI AA'CG_TT Cuts at: 1563 3492 3865 1563 Size: 1929 373 3063 Fragments arranged by size: 3063 1929 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3428 4812 5080 1632 Size: 1796 1384 268 1917 Fragments arranged by size: 1917 1796 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5168 AflIII A'CryG_T Cuts at: 1203 4264 5241 1203 Size: 3061 977 1327 Fragments arranged by size: 3061 1327 977 AgeI A'CCGG_T Cuts at: 4196 4196 Size: 5365 AhdI GACnn_n'nnGTC Cuts at: 4094 4094 Size: 5365 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5365 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2324 1184 Size: 1140 4225 AlwNI CAG_nnn'CTG Cuts at: 2468 3108 4680 2468 Size: 640 1572 3153 Fragments arranged by size: 3153 1572 640 ApaI G_GGCC'C Cuts at: 2289 2289 Size: 5365 ApaLI G'TGCA_C Cuts at: 3422 4578 3422 Size: 1156 4209 ApoI r'AATT_y Cuts at: 1493 2674 2900 3052 5161 1493 Size: 1181 226 152 2109 1697 Fragments arranged by size: 2109 1697 1181 226 152 AseI AT'TA_AT Cuts at: 160 1032 3919 160 Size: 872 2887 1606 Fragments arranged by size: 2887 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 AvaI C'yCGr_G Cuts at: 1870 1943 2290 2690 1870 Size: 73 347 400 4545 Fragments arranged by size: 4545 400 347 73 BamHI G'GATC_C Cuts at: 2695 3062 2695 Size: 367 4998 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2686 4141 618 Size: 325 217 627 899 1455 1842 Fragments arranged by size: 1842 1455 899 627 325 217 BanII G_rGCy'C Cuts at: 1391 2289 2330 2684 1391 Size: 898 41 354 4072 Fragments arranged by size: 4072 898 354 41 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4394 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2493 2527 3556 3590 1903 Size: 34 556 34 1029 34 3678 Fragments arranged by size: 3678 1029 556 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3257 4467 3257 Size: 1210 4155 BclI T'GATC_A Cuts at: 1548 1548 Size: 5365 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2054 3976 136 Size: 107 122 71 631 605 382 1922 1525 Fragments arranged by size: 1922 1525 631 605 382 122 107 71 BglII A'GATC_T Cuts at: 5360 5360 Size: 5365 BlpI GC'TnA_GC Cuts at: 2276 2782 2276 Size: 506 4859 Bme1580I G_kGCm'C Cuts at: 1790 2289 2313 3426 4582 1790 Size: 499 24 1113 1156 2573 Fragments arranged by size: 2573 1156 1113 499 24 BmgBI CAC'GTC Cuts at: 1318 2197 1318 Size: 879 4486 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2131 2215 3096 Size: 416 53 83 399 24 714 84 881 Cuts at: 3096 4054 442 Size: 958 1753 Fragments arranged by size: 1753 958 881 714 416 399 84 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5365 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2255 4025 1239 Size: 1016 1770 2579 Fragments arranged by size: 2579 1770 1016 Bpu10I CC'TnA_GC Cuts at: 2324 2324 Size: 5365 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3490 4355 4653 3490 Size: 865 298 4202 Fragments arranged by size: 4202 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4028 882 Size: 617 2529 2219 Fragments arranged by size: 2529 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3156 493 Size: 711 1952 2702 Fragments arranged by size: 2702 1952 711 BsaBI GATnn'nnATC Cuts at: 5359 5359 Size: 5365 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2057 2265 3172 3554 Size: 53 83 186 936 524 208 907 382 Cuts at: 3554 4189 275 Size: 635 1451 Fragments arranged by size: 1451 936 907 635 524 382 208 186 83 53 BsaWI w'CCGG_w Cuts at: 3798 4196 4470 4617 5028 3798 Size: 398 274 147 411 4135 Fragments arranged by size: 4135 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2117 2147 1178 Size: 30 165 30 714 30 4396 Fragments arranged by size: 4396 714 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5365 BseYI C'CCAG_C Cuts at: 4568 5064 4568 Size: 496 4869 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4792 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2107 2251 2815 3576 4604 1540 Size: 275 186 106 144 564 761 1028 2301 Fragments arranged by size: 2301 1028 761 564 275 186 144 106 BsiHKAI G_wGCw'C Cuts at: 3426 3511 4582 3426 Size: 85 1071 4209 Fragments arranged by size: 4209 1071 85 BsmI GAATG_Cn' Cuts at: 2509 2876 2969 2509 Size: 367 93 4905 Fragments arranged by size: 4905 367 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2203 2203 Size: 5365 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2289 2313 2330 2684 3426 3511 4582 Size: 399 499 24 17 354 742 85 1071 Cuts at: 4582 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 499 399 354 85 24 17 BspEI T'CCGG_A Cuts at: 5028 5028 Size: 5365 BspHI T'CATG_A Cuts at: 3254 4984 3254 Size: 1730 3635 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2361 2720 844 Size: 1517 359 3489 Fragments arranged by size: 3489 1517 359 BsrBI CCG'CTC Cuts at: 1394 1823 3252 1394 Size: 429 1429 3507 Fragments arranged by size: 3507 1429 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3860 4034 65 Size: 1183 2612 174 1396 Fragments arranged by size: 2612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2250 4009 4196 1700 Size: 110 440 1759 187 2869 Fragments arranged by size: 2869 1759 440 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5365 BssHII G'CGCG_C Cuts at: 1925 2030 2032 2171 1925 Size: 105 2 139 5119 Fragments arranged by size: 5119 139 105 2 BssSI C'ACGA_G Cuts at: 2078 3425 4437 2078 Size: 1347 1012 3006 Fragments arranged by size: 3006 1347 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5365 BstBI TT'CG_AA Cuts at: 4169 4169 Size: 5365 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5365 BstXI CCAn_nnnn'nTGG Cuts at: 2389 2389 Size: 5365 BstYI r'GATC_y Cuts at: 1972 2695 3062 3447 3464 4916 5002 5014 5360 Size: 723 367 385 17 1452 86 12 346 Cuts at: 5360 1972 Size: 1977 Fragments arranged by size: 1977 1452 723 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2020 2113 513 Size: 552 939 16 93 3765 Fragments arranged by size: 3765 939 552 93 16 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4626 Fragments arranged by size: 4626 683 56 BtsI GCAGTG_nn' Cuts at: 2036 2881 3675 3695 2036 Size: 845 794 20 3706 Fragments arranged by size: 3706 845 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5365 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5330 DraI TTT'AAA Cuts at: 2669 2669 Size: 5365 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4372 809 Size: 507 3056 1802 Fragments arranged by size: 3056 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2007 2104 2217 Size: 54 1425 50 275 18 177 97 113 Cuts at: 2217 2248 2812 3701 5330 8 Size: 31 564 889 1629 43 Fragments arranged by size: 1629 1425 889 564 275 177 113 97 54 50 43 31 18 EagI C'GGCC_G Cuts at: 1537 1812 2104 2248 2812 1537 Size: 275 292 144 564 4090 Fragments arranged by size: 4090 564 292 275 144 EarI CTCTTCn'nnn_ Cuts at: 1353 3295 1353 Size: 1942 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3952 4326 4472 387 Size: 1064 138 2363 374 146 1280 Fragments arranged by size: 2363 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2255 3428 4025 4812 5080 1239 Size: 393 623 1173 597 787 268 1524 Fragments arranged by size: 1524 1173 787 623 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2286 2599 2286 Size: 313 5052 EcoRI G'AATT_C Cuts at: 2674 3052 2674 Size: 378 4987 FseI GG_CCGG'CC Cuts at: 2254 2254 Size: 5365 FspI TGC'GCA Cuts at: 3871 3871 Size: 5365 HaeII r_GCGC'y Cuts at: 1678 2075 2214 4512 1678 Size: 397 139 2298 2531 Fragments arranged by size: 2531 2298 397 139 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2117 2149 2255 2287 2610 Size: 32 135 32 545 32 106 32 323 Cuts at: 2610 2642 4006 4038 4080 4112 1373 Size: 32 1364 32 42 32 2626 Fragments arranged by size: 2626 1364 545 323 135 106 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2061 2709 2955 669 Size: 988 281 123 648 246 3079 Fragments arranged by size: 3079 988 648 281 246 123 HindIII A'AGCT_T Cuts at: 748 748 Size: 5365 HpaI GTT'AAC Cuts at: 2955 2955 Size: 5365 KpnI G_GTAC'C Cuts at: 2690 2690 Size: 5365 MfeI C'AATT_G Cuts at: 2964 2964 Size: 5365 MluI A'CGCG_T Cuts at: 5241 5241 Size: 5365 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2226 4479 4663 1305 Size: 921 2253 184 2007 Fragments arranged by size: 2253 2007 921 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2219 10 Size: 54 1425 343 387 3156 Fragments arranged by size: 3156 1425 387 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3323 3682 3841 518 Size: 648 2157 359 159 2042 Fragments arranged by size: 2157 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2304 2332 2781 3458 4606 4851 1606 Size: 416 282 28 449 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 449 416 282 245 28 NaeI GCC'GGC Cuts at: 2252 2252 Size: 5365 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3874 Fragments arranged by size: 3874 939 552 NdeI CA'TA_TG Cuts at: 387 2368 387 Size: 1981 3384 NgoMIV G'CCGG_C Cuts at: 2250 2250 Size: 5365 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5365 NotI GC'GGCC_GC Cuts at: 2812 2812 Size: 5365 NspI r_CATG'y Cuts at: 1132 1229 2723 4268 1132 Size: 97 1494 1545 2229 Fragments arranged by size: 2229 1545 1494 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5365 PciI A'CATG_T Cuts at: 4264 4264 Size: 5365 PflMI CCAn_nnn'nTGG Cuts at: 1108 2595 1108 Size: 1487 3878 PfoI T'CCnGG_A Cuts at: 2601 2601 Size: 5365 PmeI GTTT'AAAC Cuts at: 2669 2669 Size: 5365 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3434 3466 4947 4979 3434 Size: 32 1481 32 3820 Fragments arranged by size: 3820 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2599 2599 Size: 5365 PshAI GACnn'nnGTC Cuts at: 1613 2195 1613 Size: 582 4783 PsiI TTA'TAA Cuts at: 2649 2935 2649 Size: 286 5079 PspOMI G'GGCC_C Cuts at: 2285 2285 Size: 5365 PstI C_TGCA'G Cuts at: 830 2337 2358 2717 830 Size: 1507 21 359 3478 Fragments arranged by size: 3478 1507 359 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5365 SacII CC_GC'GG Cuts at: 2023 2023 Size: 5365 SalI G'TCGA_C Cuts at: 1936 2059 2707 1936 Size: 123 648 4594 Fragments arranged by size: 4594 648 123 SbfI CC_TGCA'GG Cuts at: 2358 2717 2358 Size: 359 5006 ScaI AGT'ACT Cuts at: 3613 5325 3613 Size: 1712 3653 SfcI C'TryA_G Cuts at: 826 1045 2333 2354 2713 3848 4529 4720 826 Size: 219 1288 21 359 1135 681 191 1471 Fragments arranged by size: 1471 1288 1135 681 359 219 191 21 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5365 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5365 SmaI CCC'GGG Cuts at: 1872 2292 2692 1872 Size: 420 400 4545 Fragments arranged by size: 4545 420 400 SmlI C'TyrA_G Cuts at: 820 1017 1943 3469 4370 4632 820 Size: 197 926 1526 901 262 1553 Fragments arranged by size: 1553 1526 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3156 493 Size: 2663 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5365 SphI G_CATG'C Cuts at: 1132 2723 1132 Size: 1591 3774 SspI AAT'ATT Cuts at: 5 52 2645 3289 5 Size: 47 2593 644 2081 Fragments arranged by size: 2593 2081 644 47 StuI AGG'CCT Cuts at: 1517 2132 1517 Size: 615 4750 StyI C'CwwG_G Cuts at: 513 1065 2004 2475 2804 513 Size: 552 939 471 329 3074 Fragments arranged by size: 3074 939 552 471 329 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3392 3409 3562 3747 4192 325 Size: 1255 1812 17 153 185 445 1498 Fragments arranged by size: 1812 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3611 5323 96 Size: 274 80 33 51 1443 1634 1712 138 Fragments arranged by size: 1712 1634 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5325 5357 5325 Size: 32 5333 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5365 XbaI T'CTAG_A Cuts at: 2701 2701 Size: 5365 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2230 2230 Size: 5365 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5365 XmaI C'CCGG_G Cuts at: 1870 2290 2690 1870 Size: 420 400 4545 Fragments arranged by size: 4545 420 400 XmnI GAAnn'nnTTC Cuts at: 3494 3494 Size: 5365 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2266 3173 276 Size: 53 83 186 936 732 907 2468 Fragments arranged by size: 2468 936 907 732 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI