Restriction map for
pFN21AB8119
(Linear) (Six-base) MAPSORT of: KIBB8119.seq Check: 5779 from: 1 to: 660
KIBB8119.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AatII G_ACGT'C
Cuts at: 0 263 660
Size: 263 397
AccI GT'mk_AC
Cuts at: 0 55 660
Size: 55 605
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 319 660
Size: 319 341
AlwNI CAG_nnn'CTG
Cuts at: 0 463 660
Size: 463 197
ApaI G_GGCC'C
Cuts at: 0 284 660
Size: 284 376
AvaI C'yCGr_G
Cuts at: 0 285 660
Size: 285 375
BanII G_rGCy'C
Cuts at: 0 284 325 660
Size: 284 41 335
Fragments arranged by size:
335 284 41
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 488 522 660
Size: 488 34 138
Fragments arranged by size:
488 138 34
BglI GCCn_nnn'nGGC
Cuts at: 0 49 660
Size: 49 611
BlpI GC'TnA_GC
Cuts at: 0 271 660
Size: 271 389
Bme1580I G_kGCm'C
Cuts at: 0 284 308 660
Size: 284 24 352
Fragments arranged by size:
352 284 24
BmgBI CAC'GTC
Cuts at: 0 192 660
Size: 192 468
BmrI ACTGGGnnnn_n'
Cuts at: 0 126 210 660
Size: 126 84 450
Fragments arranged by size:
450 126 84
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 660
Size: 250 410
Bpu10I CC'TnA_GC
Cuts at: 0 319 660
Size: 319 341
BsaHI Gr'CG_yC
Cuts at: 0 52 260 660
Size: 52 208 400
Fragments arranged by size:
400 208 52
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 112 142 660
Size: 112 30 518
Fragments arranged by size:
518 112 30
BsiEI CG_ry'CG
Cuts at: 0 102 246 660
Size: 102 144 414
Fragments arranged by size:
414 144 102
BsmI GAATG_Cn'
Cuts at: 0 504 660
Size: 504 156
BsmBI CGTCTCn'nnnn_
Cuts at: 0 198 660
Size: 198 462
Bsp1286I G_dGCh'C
Cuts at: 0 284 308 325 660
Size: 284 24 17 335
Fragments arranged by size:
335 284 24 17
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 356 660
Size: 356 304
BsrFI r'CCGG_y
Cuts at: 0 245 660
Size: 245 415
BssHII G'CGCG_C
Cuts at: 0 25 27 166 660
Size: 25 2 139 494
Fragments arranged by size:
494 139 25 2
BssSI C'ACGA_G
Cuts at: 0 73 660
Size: 73 587
BstXI CCAn_nnnn'nTGG
Cuts at: 0 384 660
Size: 384 276
BtgI C'CryG_G
Cuts at: 0 15 108 660
Size: 15 93 552
Fragments arranged by size:
552 93 15
BtsI GCAGTG_nn'
Cuts at: 0 31 660
Size: 31 629
EaeI y'GGCC_r
Cuts at: 0 2 99 212 243 660
Size: 2 97 113 31 417
Fragments arranged by size:
417 113 97 31 2
EagI C'GGCC_G
Cuts at: 0 99 243 660
Size: 99 144 417
Fragments arranged by size:
417 144 99
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 250 660
Size: 250 410
EcoO109I rG'GnC_Cy
Cuts at: 0 281 594 660
Size: 281 313 66
Fragments arranged by size:
313 281 66
FseI GG_CCGG'CC
Cuts at: 0 249 660
Size: 249 411
HaeII r_GCGC'y
Cuts at: 0 70 209 660
Size: 70 139 451
Fragments arranged by size:
451 139 70
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 112 144 250 282 605 637 660
Size: 112 32 106 32 323 32 23
Fragments arranged by size:
323 112 106 32 32 32 23
HincII GTy'rAC
Cuts at: 0 56 660
Size: 56 604
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 660
Size: 221 439
MscI TGG'CCA
Cuts at: 0 214 660
Size: 214 446
MspA1I CmG'CkG
Cuts at: 0 17 299 327 660
Size: 17 282 28 333
Fragments arranged by size:
333 282 28 17
NaeI GCC'GGC
Cuts at: 0 247 660
Size: 247 413
NdeI CA'TA_TG
Cuts at: 0 363 660
Size: 363 297
NgoMIV G'CCGG_C
Cuts at: 0 245 660
Size: 245 415
PflMI CCAn_nnn'nTGG
Cuts at: 0 590 660
Size: 590 70
PfoI T'CCnGG_A
Cuts at: 0 596 660
Size: 596 64
PpuMI rG'GwC_Cy
Cuts at: 0 594 660
Size: 594 66
PshAI GACnn'nnGTC
Cuts at: 0 190 660
Size: 190 470
PsiI TTA'TAA
Cuts at: 0 644 660
Size: 644 16
PspOMI G'GGCC_C
Cuts at: 0 280 660
Size: 280 380
PstI C_TGCA'G
Cuts at: 0 332 353 660
Size: 332 21 307
Fragments arranged by size:
332 307 21
SacII CC_GC'GG
Cuts at: 0 18 660
Size: 18 642
SalI G'TCGA_C
Cuts at: 0 54 660
Size: 54 606
SbfI CC_TGCA'GG
Cuts at: 0 353 660
Size: 353 307
SfcI C'TryA_G
Cuts at: 0 328 349 660
Size: 328 21 311
Fragments arranged by size:
328 311 21
SmaI CCC'GGG
Cuts at: 0 287 660
Size: 287 373
SspI AAT'ATT
Cuts at: 0 640 660
Size: 640 20
StuI AGG'CCT
Cuts at: 0 127 660
Size: 127 533
StyI C'CwwG_G
Cuts at: 0 470 660
Size: 470 190
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 225 660
Size: 225 435
XmaI C'CCGG_G
Cuts at: 0 285 660
Size: 285 375
ZraI GAC'GTC
Cuts at: 0 261 660
Size: 261 399
Enzymes that do cut:
AatII AccI AlfI AlwNI ApaI AvaI BanII BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaHI
BsaXI BsiEI BsmI BsmBI Bsp1286I BspMI BsrFI BssHII
BssSI BstXI BtgI BtsI EaeI EagI Eco57MI EcoO109I
FseI HaeII Hin4I HincII MmeI MscI MspA1I NaeI
NdeI NgoMIV PflMI PfoI PpuMI PshAI PsiI PspOMI
PstI SacII SalI SbfI SfcI SmaI SspI StuI
StyI XcmI XmaI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BmtI BplI BpuEI BsaI BsaAI
BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BspEI
BspHI BsrBI BsrDI BsrGI BstAPI BstBI BstEII BstYI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII DrdI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FspI
FspAI HindIII HpaI KasI KpnI MfeI MluI MslI
NarI NcoI NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PpiI PspXI PsrI PvuI
PvuII RsrII SacI SanDI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmlI SnaBI SpeI SphI SrfI
SwaI TaqII TatI TstI Tth111I XbaI XhoI XmnI