Restriction map for
pFN21AB8117
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BbvCI BcgI BciVI BfrI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FseI FspI
HgaI HpaI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StyI
SwaI Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 1099 GTCGAC GT^[AC][GT]AC
AccII 8 CGCG CG^CG
AcsI 425 AAATTC [GA]^AATT[CT]
AcyI 246 GGCGCC G[GA]^CG[CT]C
AocI 1444 CCTCAGG CC^T[ACGT]AGG
ApaI 416 GGGCCC GGGCC^C
ApoI 425 AAATTC [GA]^AATT[CT]
BbeI 246 GGCGCC GGCGC^C
BbrPI 190 CACGTG CAC^GTG
BclI 252 TGATCA T^GATCA
BglII 119 AGATCT A^GATCT
BsaAI 190 CACGTG [CT]AC^GT[GA]
BsaHI 246 GGCGCC G[GA]^CG[CT]C
BsaI 781 GGTCTC GGTCTC(1/5)
BsgI 1022 GTGCAG GTGCAG(16/14)
BstAPI 936 GCAGGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 842 TTCGAA TT^CGAA
BstUI 8 CGCG CG^CG
Bsu36I 1444 CCTCAGG CC^T[ACGT]AGG
DraIII 379 CACGCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1031 CCACGG C^C[GA][CT]GG
EarI 1197 GAAGAG CTCTTC(1/4)
Eco81I 1444 CCTCAGG CC^T[ACGT]AGG
FbaI 252 TGATCA T^GATCA
Hin1I 246 GGCGCC G[GA]^CG[CT]C
HincII 1099 GTCGAC GT[CT]^[GA]AC
HindII 1099 GTCGAC GT[CT]^[GA]AC
HindIII 1126 AAGCTT A^AGCTT
KasI 246 GGCGCC G^GCGCC
Ksp632I 1197 GAAGAG CTCTTC(1/4)
MseI 713 TTAA T^TAA
MvnI 8 CGCG CG^CG
NarI 246 GGCGCC GG^CGCC
NspV 842 TTCGAA TT^CGAA
PmaCI 190 CACGTG CAC^GTG
PmlI 190 CACGTG CAC^GTG
PvuII 143 CAGCTG CAG^CTG
SalI 1099 GTCGAC G^TCGAC
SfoI 246 GGCGCC GGC^GCC
SfuI 842 TTCGAA TT^CGAA
Tru9I 713 TTAA T^TAA
Tsp509I 426 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 480 GTAC GT^AC
640 GTAC
BamHI 762 GGATCC G^GATCC
1440 GGATCC
BanI 246 GGCGCC G^G[CT][GA]CC
354 GGTGCC
BanII 416 GGGCCC G[GA]GC[CT]^C
746 GAGCCC
BseRI 436 GAGGAG GAGGAG(10/8)
1390 GAGGAG
BsmFI 344 GGGAC GGGAC(10/14)
805 GTCCC
HaeII 17 GGCGCT [GA]GCGC^[CT]
246 GGCGCC
MslI 531 CACAGCCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1224 CACAACCATG
NaeI 680 GCCGGC GCC^GGC
867 GCCGGC
NgoMIV 680 GCCGGC G^CCGGC
867 GCCGGC
NlaIII 1041 CATG CATG^
1230 CATG
PflMI 378 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
501 CCAGGAGCTGG
PleI 1120 GACTC GAGTC(4/5)
651 GAGTC
PstI 1182 CTGCAG CTGCA^G
1264 CTGCAG
RsaI 480 GTAC GT^AC
640 GTAC
SmaI 461 CCCGGG CCC^GGG
1400 CCCGGG
TfiI 540 GATTC G^A[AT]TC
732 GATTC
Van91I 378 CCACGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
501 CCAGGAGCTGG
XcmI 1031 CCACGGGAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1424 CCAGGGAGAAGCTGG
XmaCI 461 CCCGGG C^CCGGG
1400 CCCGGG
XmaI 461 CCCGGG C^CCGGG
1400 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AluI AlwI AlwNI AspHI
AvaI AvaII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsoBI Bsp1286I BspMI
BsrFI BsrI BssKI BstF5I BstNI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIV PpuMI Sau3AI Sau96I
ScrFI SfaNI SfcI StuI TaqI
TseI Tsp45I TspRI TthHB8I XhoII