Restriction map for
pFN21AB8117
(Circular) (Six-base) MAPSORT of: pFN21AB8117.seq Check: 6501 from: 1 to: 6178
pFN21AB8117 6178 bp
With 182 enzymes: SgfI *
February 27, 2009 14:40 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2181 2932 2966 2181
Size: 751 34 5393
Fragments arranged by size:
5393 751 34
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3988 278
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3105 3521 1937
Size: 1168 416 4594
Fragments arranged by size:
4594 1168 416
Acc65I G'GTAC_C
Cuts at: 3499 3499
Size: 6178
AclI AA'CG_TT
Cuts at: 1563 4305 4678 1563
Size: 2742 373 3063
Fragments arranged by size:
3063 2742 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3299 4241 5625 5893 1632
Size: 1667 942 1384 268 1917
Fragments arranged by size:
1917 1667 1384 942 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5981
AflIII A'CryG_T
Cuts at: 1203 5077 6054 1203
Size: 3874 977 1327
Fragments arranged by size:
3874 1327 977
AgeI A'CCGG_T
Cuts at: 5009 5009
Size: 6178
AhdI GACnn_n'nnGTC
Cuts at: 4907 4907
Size: 6178
AleI CACnn'nnGTG
Cuts at: 1166 2540 1166
Size: 1374 4804
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6178
AlwNI CAG_nnn'CTG
Cuts at: 2512 2947 3195 3265 3277 3334 3921 5493 2512
Size: 435 248 70 12 57 587 1572 3197
Fragments arranged by size:
3197 1572 587 435 248 70 57 12
ApaI G_GGCC'C
Cuts at: 2425 2425
Size: 6178
ApaLI G'TGCA_C
Cuts at: 4235 5391 4235
Size: 1156 5022
ApoI r'AATT_y
Cuts at: 1493 2430 3487 3713 3865 5974 1493
Size: 937 1057 226 152 2109 1697
Fragments arranged by size:
2109 1697 1057 937 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4732 160
Size: 872 3700 1606
Fragments arranged by size:
3700 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
AvaI C'yCGr_G
Cuts at: 1870 1943 2080 2264 2466 2818 3405 3503 1870
Size: 73 137 184 202 352 587 98 4545
Fragments arranged by size:
4545 587 352 202 184 137 98 73
BamHI G'GATC_C
Cuts at: 2767 3445 3508 3875 2767
Size: 678 63 367 5070
Fragments arranged by size:
5070 678 367 63
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2251 2359 3499 4954 618
Size: 325 217 627 464 108 1140 1455 1842
Fragments arranged by size:
1842 1455 1140 627 464 325 217 108
BanII G_rGCy'C
Cuts at: 1391 2425 2755 3497 1391
Size: 1034 330 742 4072
Fragments arranged by size:
4072 1034 742 330
BbeI G_GCGC'C
Cuts at: 2255 2255
Size: 6178
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2120 2672 3057 928
Size: 971 221 552 385 4049
Fragments arranged by size:
4049 971 552 385 221
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4369 4403 1903
Size: 34 2432 34 3678
Fragments arranged by size:
3678 2432 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4070 5280 4070
Size: 1210 4968
BclI T'GATC_A
Cuts at: 1548 2257 1548
Size: 709 5469
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2151 2891 3329
Size: 107 122 71 631 605 479 740 438
Cuts at: 3329 3471 4789 136
Size: 142 1318 1525
Fragments arranged by size:
1525 1318 740 631 605 479 438 142
122 107 71
BglII A'GATC_T
Cuts at: 2124 6173 2124
Size: 4049 2129
BlpI GC'TnA_GC
Cuts at: 2396 3074 3338 3595 2396
Size: 678 264 257 4979
Fragments arranged by size:
4979 678 264 257
Bme1580I G_kGCm'C
Cuts at: 1790 2226 2425 3036 3120 3419 4239 5395 1790
Size: 436 199 611 84 299 820 1156 2573
Fragments arranged by size:
2573 1156 820 611 436 299 199 84
BmgBI CAC'GTC
Cuts at: 1318 2353 2575 1318
Size: 1035 222 4921
Fragments arranged by size:
4921 1035 222
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3385 3909 4867
Size: 416 53 83 399 24 1968 524 958
Cuts at: 4867 442
Size: 1753
Fragments arranged by size:
1968 1753 958 524 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6178
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2534 2630 2774 3023 4838 1239
Size: 1295 96 144 249 1815 2579
Fragments arranged by size:
2579 1815 1295 249 144 96
Bpu10I CC'TnA_GC
Cuts at: 2525 2601 2525
Size: 76 6102
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4303 5168 5466 4303
Size: 865 298 5015
Fragments arranged by size:
5015 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2792 4841 882
Size: 617 1293 2049 2219
Fragments arranged by size:
2219 2049 1293 617
BsaAI yAC'GTr
Cuts at: 493 1204 2197 3969 493
Size: 711 993 1772 2702
Fragments arranged by size:
2702 1772 993 711
BsaBI GATnn'nnATC
Cuts at: 6172 6172
Size: 6178
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2252 3985 4367 5002
Size: 53 83 186 936 719 1733 382 635
Cuts at: 5002 275
Size: 1451
Fragments arranged by size:
1733 1451 936 719 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4611 5009 5283 5430 5841 4611
Size: 398 274 147 411 4948
Fragments arranged by size:
4948 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2084 2114 1178
Size: 30 165 30 681 30 5242
Fragments arranged by size:
5242 681 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2456 3410 1186
Size: 1270 954 3954
Fragments arranged by size:
3954 1270 954
BseYI C'CCAG_C
Cuts at: 2146 2937 3195 3284 5381 5877 2146
Size: 791 258 89 2097 496 2447
Fragments arranged by size:
2447 2097 791 496 258 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3048 1163
Size: 573 1312 4293
Fragments arranged by size:
4293 1312 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3628 4389 5417 1540
Size: 275 186 1627 761 1028 2301
Fragments arranged by size:
2301 1627 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2078 2462 2607 3075 4239 4324 5395 2078
Size: 384 145 468 1164 85 1071 2861
Fragments arranged by size:
2861 1164 1071 468 384 145 85
BsmI GAATG_Cn'
Cuts at: 3689 3782 3689
Size: 93 6085
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2078 2226 2425 2462 2607 2755 3036
Size: 399 288 148 199 37 145 148 281
Cuts at: 3036 3075 3120 3419 3497 4239 4324 5395 1391
Size: 39 45 299 78 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 399 299 288 281 199
148 148 145 85 78 45 39 37
BspEI T'CCGG_A
Cuts at: 5841 5841
Size: 6178
BspHI T'CATG_A
Cuts at: 4067 5797 4067
Size: 1730 4448
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2181 2932 2966 3533 844
Size: 1337 751 34 567 3489
Fragments arranged by size:
3489 1337 751 567 34
BsrBI CCG'CTC
Cuts at: 1394 1823 4065 1394
Size: 429 2242 3507
Fragments arranged by size:
3507 2242 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4673 4847 65
Size: 1183 3425 174 1396
Fragments arranged by size:
3425 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2685 2872 3254 4822 5009 1700
Size: 110 875 187 382 1568 187 2869
Fragments arranged by size:
2869 1568 875 382 187 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6178
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6178
BssSI C'ACGA_G
Cuts at: 4238 5250 4238
Size: 1012 5166
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2947 794
Size: 2153 4025
BstBI TT'CG_AA
Cuts at: 2848 4982 2848
Size: 2134 4044
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6178
BstYI r'GATC_y
Cuts at: 1972 2124 2767 3445 3508 3875 4260 4277 5729
Size: 152 643 678 63 367 385 17 1452
Cuts at: 5729 5815 5827 6173 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 678 643 385 367 346 152
86 63 17 12
Bsu36I CC'TnA_GG
Cuts at: 3450 3450
Size: 6178
BtgI C'CryG_G
Cuts at: 513 1065 2004 3036 513
Size: 552 939 1032 3655
Fragments arranged by size:
3655 1032 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5439
Fragments arranged by size:
5439 683 56
BtsI GCAGTG_nn'
Cuts at: 2146 3036 3694 4488 4508 2146
Size: 890 658 794 20 3816
Fragments arranged by size:
3816 890 794 658 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6178
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6143
DraI TTT'AAA
Cuts at: 3482 3482
Size: 6178
DraIII CAC_nnn'GTG
Cuts at: 2389 2389
Size: 6178
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5185 809
Size: 507 3869 1802
Fragments arranged by size:
3869 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2311 2853 3256
Size: 54 1425 50 275 18 481 542 403
Cuts at: 3256 3356 3625 4514 6143 8
Size: 100 269 889 1629 43
Fragments arranged by size:
1629 1425 889 542 481 403 275 269
100 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3625 1537
Size: 275 1813 4090
Fragments arranged by size:
4090 1813 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3197 4108 1353
Size: 1844 911 3423
Fragments arranged by size:
3423 1844 911
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2452 2891 2972 4765 5139 5285
Size: 1064 138 863 439 81 1793 374 146
Cuts at: 5285 387
Size: 1280
Fragments arranged by size:
1793 1280 1064 863 439 374 146 138
81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2534 2630 2774 3023 3299 4241 4838
Size: 393 902 96 144 249 276 942 597
Cuts at: 4838 5625 5893 1239
Size: 787 268 1524
Fragments arranged by size:
1524 942 902 787 597 393 276 268
249 144 96
EcoO109I rG'GnC_Cy
Cuts at: 2422 2809 2914 3209 3274 3331 2422
Size: 387 105 295 65 57 5269
Fragments arranged by size:
5269 387 295 105 65 57
EcoRI G'AATT_C
Cuts at: 3487 3865 3487
Size: 378 5800
FspI TGC'GCA
Cuts at: 4684 4684
Size: 6178
HaeII r_GCGC'y
Cuts at: 1678 2026 2255 5325 1678
Size: 348 229 3070 2531
Fragments arranged by size:
3070 2531 348 229
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2641 2673 4819 4851 4893
Size: 32 135 32 1069 32 2146 32 42
Cuts at: 4893 4925 1373
Size: 32 2626
Fragments arranged by size:
2626 2146 1069 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3106 3522 3768 669
Size: 988 281 1168 416 246 3079
Fragments arranged by size:
3079 1168 988 416 281 246
HindIII A'AGCT_T
Cuts at: 748 3131 748
Size: 2383 3795
HpaI GTT'AAC
Cuts at: 3768 3768
Size: 6178
KasI G'GCGC_C
Cuts at: 2251 2251
Size: 6178
KpnI G_GTAC'C
Cuts at: 3503 3503
Size: 6178
MfeI C'AATT_G
Cuts at: 3777 3777
Size: 6178
MluI A'CGCG_T
Cuts at: 6054 6054
Size: 6178
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5292 5476 1305
Size: 3987 184 2007
Fragments arranged by size:
3987 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4356
Fragments arranged by size:
4356 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2540 3233 4136 4495 4654 518
Size: 648 1374 693 903 359 159 2042
Fragments arranged by size:
2042 1374 903 693 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2150 2316 3192 3594 4271 5419 5664 1606
Size: 544 166 876 402 677 1148 245 2120
Fragments arranged by size:
2120 1148 876 677 544 402 245 166
NaeI GCC'GGC
Cuts at: 2687 2874 2687
Size: 187 5991
NarI GG'CG_CC
Cuts at: 2252 2252
Size: 6178
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4687
Fragments arranged by size:
4687 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6178
NgoMIV G'CCGG_C
Cuts at: 2685 2872 2685
Size: 187 5991
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6178
NotI GC'GGCC_GC
Cuts at: 3625 3625
Size: 6178
NspI r_CATG'y
Cuts at: 1132 1229 3536 5081 1132
Size: 97 2307 1545 2229
Fragments arranged by size:
2307 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2638 3429 1348
Size: 1290 791 4097
Fragments arranged by size:
4097 1290 791
PciI A'CATG_T
Cuts at: 5077 5077
Size: 6178
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2389 2512 1108
Size: 1281 123 4774
Fragments arranged by size:
4774 1281 123
PfoI T'CCnGG_A
Cuts at: 2790 2790
Size: 6178
PmeI GTTT'AAAC
Cuts at: 3482 3482
Size: 6178
PmlI CAC'GTG
Cuts at: 2197 2197
Size: 6178
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4247 4279 5760 5792 4247
Size: 32 1481 32 4633
Fragments arranged by size:
4633 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2809 3209 3274 2809
Size: 400 65 5713
Fragments arranged by size:
5713 400 65
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6178
PsiI TTA'TAA
Cuts at: 3748 3748
Size: 6178
PspOMI G'GGCC_C
Cuts at: 2421 2421
Size: 6178
PstI C_TGCA'G
Cuts at: 830 3191 3273 3530 830
Size: 2361 82 257 3478
Fragments arranged by size:
3478 2361 257 82
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6178
PvuII CAG'CTG
Cuts at: 2150 2150
Size: 6178
SalI G'TCGA_C
Cuts at: 1936 3104 3520 1936
Size: 1168 416 4594
Fragments arranged by size:
4594 1168 416
SbfI CC_TGCA'GG
Cuts at: 3530 3530
Size: 6178
ScaI AGT'ACT
Cuts at: 4426 6138 4426
Size: 1712 4466
SfcI C'TryA_G
Cuts at: 826 1045 3187 3213 3269 3526 4661 5342 5533
Size: 219 2142 26 56 257 1135 681 191
Cuts at: 5533 826
Size: 1471
Fragments arranged by size:
2142 1471 1135 681 257 219 191 56
26
SfoI GGC'GCC
Cuts at: 2253 2253
Size: 6178
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6178
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6178
SmaI CCC'GGG
Cuts at: 1872 2468 3407 3505 1872
Size: 596 939 98 4545
Fragments arranged by size:
4545 939 596 98
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4282 5183 5445 820
Size: 197 926 2339 901 262 1553
Fragments arranged by size:
2339 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3969 493
Size: 3476 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6178
SphI G_CATG'C
Cuts at: 1132 3536 1132
Size: 2404 3774
SrfI GCCC'GGGC
Cuts at: 2468 2468
Size: 6178
SspI AAT'ATT
Cuts at: 5 52 4102 5
Size: 47 4050 2081
Fragments arranged by size:
4050 2081 47
StuI AGG'CCT
Cuts at: 1517 2378 3180 3310 1517
Size: 861 802 130 4385
Fragments arranged by size:
4385 861 802 130
StyI C'CwwG_G
Cuts at: 513 1065 2004 3617 513
Size: 552 939 1613 3074
Fragments arranged by size:
3074 1613 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2630 3187 4205 4222 4375 4560 5005
Size: 1255 1050 557 1018 17 153 185 445
Cuts at: 5005 325
Size: 1498
Fragments arranged by size:
1498 1255 1050 1018 557 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2644 4424 6136
Size: 274 80 33 51 1443 667 1780 1712
Cuts at: 6136 96
Size: 138
Fragments arranged by size:
1780 1712 1443 667 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2084 2116 6138 6170 2084
Size: 32 4022 32 2092
Fragments arranged by size:
4022 2092 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6178
XbaI T'CTAG_A
Cuts at: 3514 3514
Size: 6178
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3043 3436 3043
Size: 393 5785
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6178
XmaI C'CCGG_G
Cuts at: 1870 2466 3405 3503 1870
Size: 596 939 98 4545
Fragments arranged by size:
4545 939 596 98
XmnI GAAnn'nnTTC
Cuts at: 4307 4307
Size: 6178
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3986 276
Size: 53 83 186 936 2452 2468
Fragments arranged by size:
2468 2452 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI
BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI
FseI FspAI NruI NsiI PacI PspXI PsrI RsrII
SacI SacII SanDI SapI SexAI SfiI SwaI