Restriction map for
pFN21AB8117
(Linear) (Six-base) MAPSORT of: KIBB8117.seq Check: 845 from: 1 to: 1473
KIBB8117.orf
With 182 enzymes: SgfI *
March 3, 2008 11:03 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 176 927 961 1473
Size: 176 751 34 512
Fragments arranged by size:
751 512 176 34
AccI GT'mk_AC
Cuts at: 0 1100 1473
Size: 1100 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1294 1473
Size: 1294 179
AleI CACnn'nnGTG
Cuts at: 0 535 1473
Size: 535 938
AlwNI CAG_nnn'CTG
Cuts at: 0 507 942 1190 1260 1272 1329 1473
Size: 507 435 248 70 12 57 144
Fragments arranged by size:
507 435 248 144 70 57 12
ApaI G_GGCC'C
Cuts at: 0 420 1473
Size: 420 1053
ApoI r'AATT_y
Cuts at: 0 425 1473
Size: 425 1048
AvaI C'yCGr_G
Cuts at: 0 75 259 461 813 1400 1473
Size: 75 184 202 352 587 73
Fragments arranged by size:
587 352 202 184 75 73
BamHI G'GATC_C
Cuts at: 0 762 1440 1473
Size: 762 678 33
Fragments arranged by size:
762 678 33
BanI G'GyrC_C
Cuts at: 0 246 354 1473
Size: 246 108 1119
Fragments arranged by size:
1119 246 108
BanII G_rGCy'C
Cuts at: 0 420 750 1473
Size: 420 330 723
Fragments arranged by size:
723 420 330
BbeI G_GCGC'C
Cuts at: 0 250 1473
Size: 250 1223
BbsI GAAGACnn'nnnn_
Cuts at: 0 115 667 1052 1473
Size: 115 552 385 421
Fragments arranged by size:
552 421 385 115
BclI T'GATC_A
Cuts at: 0 252 1473
Size: 252 1221
BglI GCCn_nnn'nGGC
Cuts at: 0 146 886 1324 1466 1473
Size: 146 740 438 142 7
Fragments arranged by size:
740 438 146 142 7
BglII A'GATC_T
Cuts at: 0 119 1473
Size: 119 1354
BlpI GC'TnA_GC
Cuts at: 0 391 1069 1333 1473
Size: 391 678 264 140
Fragments arranged by size:
678 391 264 140
Bme1580I G_kGCm'C
Cuts at: 0 221 420 1031 1115 1414 1473
Size: 221 199 611 84 299 59
Fragments arranged by size:
611 299 221 199 84 59
BmgBI CAC'GTC
Cuts at: 0 348 570 1473
Size: 348 222 903
Fragments arranged by size:
903 348 222
BmrI ACTGGGnnnn_n'
Cuts at: 0 1380 1473
Size: 1380 93
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 625 769 1018 1473
Size: 529 96 144 249 455
Fragments arranged by size:
529 455 249 144 96
Bpu10I CC'TnA_GC
Cuts at: 0 520 596 1473
Size: 520 76 877
Fragments arranged by size:
877 520 76
BsaI GGTCTCn'nnnn_
Cuts at: 0 787 1473
Size: 787 686
BsaAI yAC'GTr
Cuts at: 0 192 1473
Size: 192 1281
BsaHI Gr'CG_yC
Cuts at: 0 247 1473
Size: 247 1226
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 79 109 1473
Size: 79 30 1364
Fragments arranged by size:
1364 79 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 451 1405 1473
Size: 451 954 68
Fragments arranged by size:
954 451 68
BseYI C'CCAG_C
Cuts at: 0 141 932 1190 1279 1473
Size: 141 791 258 89 194
Fragments arranged by size:
791 258 194 141 89
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1043 1473
Size: 1043 430
BsiHKAI G_wGCw'C
Cuts at: 0 73 457 602 1070 1473
Size: 73 384 145 468 403
Fragments arranged by size:
468 403 384 145 73
Bsp1286I G_dGCh'C
Cuts at: 0 73 221 420 457 602 750 1031 1070
Size: 73 148 199 37 145 148 281 39
Cuts at: 1070 1115 1414 1473
Size: 45 299 59
Fragments arranged by size:
299 281 199 148 148 145 73 59
45 39 37
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 176 927 961 1473
Size: 176 751 34 512
Fragments arranged by size:
751 512 176 34
BsrFI r'CCGG_y
Cuts at: 0 680 867 1249 1473
Size: 680 187 382 224
Fragments arranged by size:
680 382 224 187
BstAPI GCAn_nnn'nTGC
Cuts at: 0 942 1473
Size: 942 531
BstBI TT'CG_AA
Cuts at: 0 843 1473
Size: 843 630
BstYI r'GATC_y
Cuts at: 0 119 762 1440 1473
Size: 119 643 678 33
Fragments arranged by size:
678 643 119 33
Bsu36I CC'TnA_GG
Cuts at: 0 1445 1473
Size: 1445 28
BtgI C'CryG_G
Cuts at: 0 1031 1473
Size: 1031 442
BtsI GCAGTG_nn'
Cuts at: 0 141 1031 1473
Size: 141 890 442
Fragments arranged by size:
890 442 141
DraIII CAC_nnn'GTG
Cuts at: 0 384 1473
Size: 384 1089
EaeI y'GGCC_r
Cuts at: 0 306 848 1251 1351 1473
Size: 306 542 403 100 122
Fragments arranged by size:
542 403 306 122 100
EarI CTCTTCn'nnn_
Cuts at: 0 1192 1473
Size: 1192 281
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 447 886 967 1473
Size: 447 439 81 506
Fragments arranged by size:
506 447 439 81
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 625 769 1018 1294 1473
Size: 529 96 144 249 276 179
Fragments arranged by size:
529 276 249 179 144 96
EcoO109I rG'GnC_Cy
Cuts at: 0 417 804 909 1204 1269 1326 1473
Size: 417 387 105 295 65 57 147
Fragments arranged by size:
417 387 295 147 105 65 57
HaeII r_GCGC'y
Cuts at: 0 21 250 1473
Size: 21 229 1223
Fragments arranged by size:
1223 229 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 636 668 1473
Size: 636 32 805
Fragments arranged by size:
805 636 32
HincII GTy'rAC
Cuts at: 0 1101 1473
Size: 1101 372
HindIII A'AGCT_T
Cuts at: 0 1126 1473
Size: 1126 347
KasI G'GCGC_C
Cuts at: 0 246 1473
Size: 246 1227
MslI CAynn'nnrTG
Cuts at: 0 535 1228 1473
Size: 535 693 245
Fragments arranged by size:
693 535 245
MspA1I CmG'CkG
Cuts at: 0 145 311 1187 1473
Size: 145 166 876 286
Fragments arranged by size:
876 286 166 145
NaeI GCC'GGC
Cuts at: 0 682 869 1473
Size: 682 187 604
Fragments arranged by size:
682 604 187
NarI GG'CG_CC
Cuts at: 0 247 1473
Size: 247 1226
NgoMIV G'CCGG_C
Cuts at: 0 680 867 1473
Size: 680 187 606
Fragments arranged by size:
680 606 187
PasI CC'CwG_GG
Cuts at: 0 633 1424 1473
Size: 633 791 49
Fragments arranged by size:
791 633 49
PflMI CCAn_nnn'nTGG
Cuts at: 0 384 507 1473
Size: 384 123 966
Fragments arranged by size:
966 384 123
PfoI T'CCnGG_A
Cuts at: 0 785 1473
Size: 785 688
PmlI CAC'GTG
Cuts at: 0 192 1473
Size: 192 1281
PpuMI rG'GwC_Cy
Cuts at: 0 804 1204 1269 1473
Size: 804 400 65 204
Fragments arranged by size:
804 400 204 65
PspOMI G'GGCC_C
Cuts at: 0 416 1473
Size: 416 1057
PstI C_TGCA'G
Cuts at: 0 1186 1268 1473
Size: 1186 82 205
Fragments arranged by size:
1186 205 82
PvuII CAG'CTG
Cuts at: 0 145 1473
Size: 145 1328
SalI G'TCGA_C
Cuts at: 0 1099 1473
Size: 1099 374
SfcI C'TryA_G
Cuts at: 0 1182 1208 1264 1473
Size: 1182 26 56 209
Fragments arranged by size:
1182 209 56 26
SfoI GGC'GCC
Cuts at: 0 248 1473
Size: 248 1225
SmaI CCC'GGG
Cuts at: 0 463 1402 1473
Size: 463 939 71
Fragments arranged by size:
939 463 71
SrfI GCCC'GGGC
Cuts at: 0 463 1473
Size: 463 1010
StuI AGG'CCT
Cuts at: 0 373 1175 1305 1473
Size: 373 802 130 168
Fragments arranged by size:
802 373 168 130
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 625 1182 1473
Size: 625 557 291
Fragments arranged by size:
625 557 291
TatI w'GTAC_w
Cuts at: 0 639 1473
Size: 639 834
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 79 111 1473
Size: 79 32 1362
Fragments arranged by size:
1362 79 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1038 1431 1473
Size: 1038 393 42
Fragments arranged by size:
1038 393 42
XmaI C'CCGG_G
Cuts at: 0 461 1400 1473
Size: 461 939 73
Fragments arranged by size:
939 461 73
Enzymes that do cut:
AarI AccI AcuI AleI AlwNI ApaI ApoI AvaI
BamHI BanI BanII BbeI BbsI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI
BsrFI BstAPI BstBI BstYI Bsu36I BtgI BtsI DraIII
EaeI EarI EciI Eco57MI EcoO109I HaeII Hin4I HincII
HindIII KasI MslI MspA1I NaeI NarI NgoMIV PasI
PflMI PfoI PmlI PpuMI PspOMI PstI PvuII SalI
SfcI SfoI SmaI SrfI StuI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BbvCI BcgI BciVI BfrBI BmtI BplI BpuEI BsaBI
BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI
BsrDI BsrGI BssHII BssSI BstEII BstXI BstZ17I BtgZI
ClaI CspCI DraI DrdI EagI EcoICRI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HpaI KpnI MfeI
MluI MmeI MscI NcoI NdeI NheI NotI NruI
NsiI NspI PacI PciI PmeI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SspI StyI SwaI Tth111I XbaI XhoI
XmnI ZraI