(Circular) (Six-base) MAPSORT of: pFN21AB8116.seq Check: 485 from: 1 to: 5464 pFN21AB8116 5464 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3274 278 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2132 2807 1937 Size: 195 675 4594 Fragments arranged by size: 4594 675 195 Acc65I G'GTAC_C Cuts at: 2785 2785 Size: 5464 AclI AA'CG_TT Cuts at: 1563 3591 3964 1563 Size: 2028 373 3063 Fragments arranged by size: 3063 2028 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2616 3527 4911 5179 1632 Size: 984 911 1384 268 1917 Fragments arranged by size: 1917 1384 984 911 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5267 AflIII A'CryG_T Cuts at: 1203 4363 5340 1203 Size: 3160 977 1327 Fragments arranged by size: 3160 1327 977 AgeI A'CCGG_T Cuts at: 4295 4295 Size: 5464 AhdI GACnn_n'nnGTC Cuts at: 4193 4193 Size: 5464 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5464 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2123 1184 Size: 939 4525 AlwNI CAG_nnn'CTG Cuts at: 2539 2654 3207 4779 2539 Size: 115 553 1572 3224 Fragments arranged by size: 3224 1572 553 115 ApaI G_GGCC'C Cuts at: 2553 2553 Size: 5464 ApaLI G'TGCA_C Cuts at: 3521 4677 3521 Size: 1156 4308 ApoI r'AATT_y Cuts at: 1493 2773 2999 3151 5260 1493 Size: 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 226 152 AseI AT'TA_AT Cuts at: 160 1032 4018 160 Size: 872 2986 1606 Fragments arranged by size: 2986 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 AvaI C'yCGr_G Cuts at: 1870 1943 2789 1870 Size: 73 846 4545 Fragments arranged by size: 4545 846 73 BamHI G'GATC_C Cuts at: 2794 3161 2794 Size: 367 5097 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2067 2785 4240 618 Size: 325 217 627 280 718 1455 1842 Fragments arranged by size: 1842 1455 718 627 325 280 217 BanII G_rGCy'C Cuts at: 1391 2128 2553 2783 1391 Size: 737 425 230 4072 Fragments arranged by size: 4072 737 425 230 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2656 928 Size: 971 757 3736 Fragments arranged by size: 3736 971 757 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3655 3689 1903 Size: 34 50 34 1634 34 3678 Fragments arranged by size: 3678 1634 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3356 4566 3356 Size: 1210 4254 BclI T'GATC_A Cuts at: 1548 1548 Size: 5464 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4075 136 Size: 107 122 71 631 605 2403 1525 Fragments arranged by size: 2403 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5459 5459 Size: 5464 BlpI GC'TnA_GC Cuts at: 2881 2881 Size: 5464 Bme1580I G_kGCm'C Cuts at: 1790 2553 3525 4681 1790 Size: 763 972 1156 2573 Fragments arranged by size: 2573 1156 972 763 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5464 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2340 3195 4153 Size: 416 53 83 399 24 923 855 958 Cuts at: 4153 442 Size: 1753 Fragments arranged by size: 1753 958 923 855 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5464 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2216 2705 2726 4124 1239 Size: 977 489 21 1398 2579 Fragments arranged by size: 2579 1398 977 489 21 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3589 4454 4752 3589 Size: 865 298 4301 Fragments arranged by size: 4301 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4127 882 Size: 617 2628 2219 Fragments arranged by size: 2628 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3255 493 Size: 711 2051 2702 Fragments arranged by size: 2702 2051 711 BsaBI GATnn'nnATC Cuts at: 5458 5458 Size: 5464 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3271 3653 4288 275 Size: 53 83 186 936 1738 382 635 1451 Fragments arranged by size: 1738 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3897 4295 4569 4716 5127 3897 Size: 398 274 147 411 4234 Fragments arranged by size: 4234 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5239 Fragments arranged by size: 5239 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2363 1186 Size: 1177 4287 BseYI C'CCAG_C Cuts at: 2162 2715 4667 5163 2162 Size: 553 1952 496 2463 Fragments arranged by size: 2463 1952 553 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2555 1163 Size: 573 819 4072 Fragments arranged by size: 4072 819 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2914 3675 4703 1540 Size: 275 186 913 761 1028 2301 Fragments arranged by size: 2301 1028 913 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2490 3525 3610 4681 2490 Size: 1035 85 1071 3273 Fragments arranged by size: 3273 1071 1035 85 BsmI GAATG_Cn' Cuts at: 2975 3068 2975 Size: 93 5371 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2128 2490 2553 2783 3525 3610 4681 Size: 399 338 362 63 230 742 85 1071 Cuts at: 4681 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 362 338 230 85 63 BspEI T'CCGG_A Cuts at: 5127 5127 Size: 5464 BspHI T'CATG_A Cuts at: 3353 5083 3353 Size: 1730 3734 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2244 2819 844 Size: 1400 575 3489 Fragments arranged by size: 3489 1400 575 BsrBI CCG'CTC Cuts at: 1394 1823 3351 1394 Size: 429 1528 3507 Fragments arranged by size: 3507 1528 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2509 3959 4133 65 Size: 1183 1261 1450 174 1396 Fragments arranged by size: 1450 1396 1261 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4108 4295 1700 Size: 110 2298 187 2869 Fragments arranged by size: 2869 2298 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5464 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5464 BssSI C'ACGA_G Cuts at: 3524 4536 3524 Size: 1012 4452 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5464 BstBI TT'CG_AA Cuts at: 4268 4268 Size: 5464 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5464 BstYI r'GATC_y Cuts at: 1972 2428 2794 3161 3546 3563 5015 5101 5113 Size: 456 366 367 385 17 1452 86 12 Cuts at: 5113 5459 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 456 385 367 366 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2358 2358 Size: 5464 BtgI C'CryG_G Cuts at: 513 1065 2119 513 Size: 552 1054 3858 Fragments arranged by size: 3858 1054 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4725 Fragments arranged by size: 4725 683 56 BtsI GCAGTG_nn' Cuts at: 2259 2385 2980 3774 3794 2259 Size: 126 595 794 20 3929 Fragments arranged by size: 3929 794 595 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5464 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5429 DraI TTT'AAA Cuts at: 2768 2768 Size: 5464 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4471 809 Size: 507 3155 1802 Fragments arranged by size: 3155 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2116 2543 2911 Size: 54 1425 50 275 18 286 427 368 Cuts at: 2911 3800 5429 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 427 368 286 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2911 1537 Size: 275 1099 4090 Fragments arranged by size: 4090 1099 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2703 2760 3394 1353 Size: 1350 57 634 3423 Fragments arranged by size: 3423 1350 634 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4051 4425 4571 387 Size: 1064 138 2462 374 146 1280 Fragments arranged by size: 2462 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2216 2616 2705 2726 3527 4124 4911 Size: 393 584 400 89 21 801 597 787 Cuts at: 4911 5179 1239 Size: 268 1524 Fragments arranged by size: 1524 801 787 597 584 400 393 268 89 21 EcoO109I rG'GnC_Cy Cuts at: 2362 2519 2549 2550 2362 Size: 157 30 1 5276 Fragments arranged by size: 5276 157 30 1 EcoRI G'AATT_C Cuts at: 2773 3151 2773 Size: 378 5086 FspI TGC'GCA Cuts at: 3970 3970 Size: 5464 HaeII r_GCGC'y Cuts at: 1678 4611 1678 Size: 2933 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2425 2457 4105 4137 4179 Size: 32 135 32 853 32 1648 32 42 Cuts at: 4179 4211 1373 Size: 32 2626 Fragments arranged by size: 2626 1648 853 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2808 3054 669 Size: 988 281 870 246 3079 Fragments arranged by size: 3079 988 870 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5464 HpaI GTT'AAC Cuts at: 3054 3054 Size: 5464 KpnI G_GTAC'C Cuts at: 2789 2789 Size: 5464 MfeI C'AATT_G Cuts at: 3063 3063 Size: 5464 MluI A'CGCG_T Cuts at: 5340 5340 Size: 5464 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2133 4578 4762 1305 Size: 828 2445 184 2007 Fragments arranged by size: 2445 2007 828 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2118 2545 10 Size: 54 1425 343 286 427 2929 Fragments arranged by size: 2929 1425 427 343 286 54 MslI CAynn'nnrTG Cuts at: 518 1166 3422 3781 3940 518 Size: 648 2256 359 159 2042 Fragments arranged by size: 2256 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2452 2536 2880 3557 4705 4950 1606 Size: 846 84 344 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 677 344 245 84 NcoI C'CATG_G Cuts at: 513 1065 2119 513 Size: 552 1054 3858 Fragments arranged by size: 3858 1054 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5464 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5464 NotI GC'GGCC_GC Cuts at: 2911 2911 Size: 5464 NspI r_CATG'y Cuts at: 1132 1229 2211 2822 4367 1132 Size: 97 982 611 1545 2229 Fragments arranged by size: 2229 1545 982 611 97 PasI CC'CwG_GG Cuts at: 1348 2042 1348 Size: 694 4770 PciI A'CATG_T Cuts at: 4363 4363 Size: 5464 PflMI CCAn_nnn'nTGG Cuts at: 1108 2614 1108 Size: 1506 3958 PfoI T'CCnGG_A Cuts at: 2721 2721 Size: 5464 PmeI GTTT'AAAC Cuts at: 2768 2768 Size: 5464 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3533 3565 5046 5078 3533 Size: 32 1481 32 3919 Fragments arranged by size: 3919 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2519 2519 Size: 5464 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5464 PsiI TTA'TAA Cuts at: 3034 3034 Size: 5464 PspOMI G'GGCC_C Cuts at: 2549 2549 Size: 5464 PstI C_TGCA'G Cuts at: 830 2090 2325 2673 2816 830 Size: 1260 235 348 143 3478 Fragments arranged by size: 3478 1260 348 235 143 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5464 PvuII CAG'CTG Cuts at: 2452 2452 Size: 5464 SalI G'TCGA_C Cuts at: 1936 2806 1936 Size: 870 4594 SapI GCTCTTCn'nnn_ Cuts at: 2760 2760 Size: 5464 SbfI CC_TGCA'GG Cuts at: 2816 2816 Size: 5464 ScaI AGT'ACT Cuts at: 3712 5424 3712 Size: 1712 3752 SfcI C'TryA_G Cuts at: 826 1045 2086 2321 2669 2812 3947 4628 4819 Size: 219 1041 235 348 143 1135 681 191 Cuts at: 4819 826 Size: 1471 Fragments arranged by size: 1471 1135 1041 681 348 235 219 191 143 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5464 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5464 SmaI CCC'GGG Cuts at: 1872 2791 1872 Size: 919 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3568 4469 4731 820 Size: 197 926 1625 901 262 1553 Fragments arranged by size: 1625 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3255 493 Size: 2762 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5464 SphI G_CATG'C Cuts at: 1132 2211 2822 1132 Size: 1079 611 3774 Fragments arranged by size: 3774 1079 611 SspI AAT'ATT Cuts at: 5 52 3388 5 Size: 47 3336 2081 Fragments arranged by size: 3336 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5464 StyI C'CwwG_G Cuts at: 513 1065 2119 2903 513 Size: 552 1054 784 3074 Fragments arranged by size: 3074 1054 784 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3491 3508 3661 3846 4291 325 Size: 1255 1911 17 153 185 445 1498 Fragments arranged by size: 1911 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3710 5422 96 Size: 274 80 33 51 1443 1733 1712 138 Fragments arranged by size: 1733 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5424 5456 5424 Size: 32 5432 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5464 XbaI T'CTAG_A Cuts at: 2800 2800 Size: 5464 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5464 XmaI C'CCGG_G Cuts at: 1870 2789 1870 Size: 919 4545 XmnI GAAnn'nnTTC Cuts at: 3593 3593 Size: 5464 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3272 276 Size: 53 83 186 936 1738 2468 Fragments arranged by size: 2468 1738 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI