(Circular) (Six-base) MAPSORT of: pFN21AB8108.seq Check: 5749 from: 1 to: 6139 pFN21AB8108 6139 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3949 278 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3482 1937 Size: 1545 4594 Acc65I G'GTAC_C Cuts at: 3460 3460 Size: 6139 AclI AA'CG_TT Cuts at: 1563 4266 4639 1563 Size: 2703 373 3063 Fragments arranged by size: 3063 2703 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3320 4202 5586 5854 1632 Size: 1688 882 1384 268 1917 Fragments arranged by size: 1917 1688 1384 882 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5942 AflIII A'CryG_T Cuts at: 1203 3175 3404 5038 6015 1203 Size: 1972 229 1634 977 1327 Fragments arranged by size: 1972 1634 1327 977 229 AgeI A'CCGG_T Cuts at: 4970 4970 Size: 6139 AhdI GACnn_n'nnGTC Cuts at: 4868 4868 Size: 6139 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6139 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6139 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2319 2351 2871 2903 3372 3404 2319 Size: 32 520 32 469 32 5054 Fragments arranged by size: 5054 520 469 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3882 5454 3882 Size: 1572 4567 ApaLI G'TGCA_C Cuts at: 4196 5352 4196 Size: 1156 4983 ApoI r'AATT_y Cuts at: 1493 3160 3448 3674 3826 5935 1493 Size: 1667 288 226 152 2109 1697 Fragments arranged by size: 2109 1697 1667 288 226 152 AseI AT'TA_AT Cuts at: 160 1032 4693 160 Size: 872 3661 1606 Fragments arranged by size: 3661 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 AvaI C'yCGr_G Cuts at: 1870 1943 3464 1870 Size: 73 1521 4545 Fragments arranged by size: 4545 1521 73 BamHI G'GATC_C Cuts at: 2059 3469 3836 2059 Size: 1410 367 4362 Fragments arranged by size: 4362 1410 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3460 4915 618 Size: 325 217 627 1673 1455 1842 Fragments arranged by size: 1842 1673 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3458 1391 Size: 2067 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2613 2963 928 Size: 971 714 350 4104 Fragments arranged by size: 4104 971 714 350 BbvCI CC'TCA_GC Cuts at: 2097 2097 Size: 6139 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4330 4364 1903 Size: 34 2393 34 3678 Fragments arranged by size: 3678 2393 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2385 4031 5241 2385 Size: 1646 1210 3283 Fragments arranged by size: 3283 1646 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6139 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2093 3187 4750 Size: 107 122 71 631 605 421 1094 1563 Cuts at: 4750 136 Size: 1525 Fragments arranged by size: 1563 1525 1094 631 605 421 122 107 71 BglII A'GATC_T Cuts at: 6134 6134 Size: 6139 BlpI GC'TnA_GC Cuts at: 3556 3556 Size: 6139 Bme1580I G_kGCm'C Cuts at: 1790 4200 5356 1790 Size: 2410 1156 2573 Fragments arranged by size: 2573 2410 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6139 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3870 4828 442 Size: 416 53 83 399 24 2453 958 1753 Fragments arranged by size: 2453 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6139 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4799 1239 Size: 3560 2579 Bpu10I CC'TnA_GC Cuts at: 2097 2097 Size: 6139 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4264 5129 5427 4264 Size: 865 298 4976 Fragments arranged by size: 4976 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4802 882 Size: 617 3303 2219 Fragments arranged by size: 3303 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2551 3930 493 Size: 711 1347 1379 2702 Fragments arranged by size: 2702 1379 1347 711 BsaBI GATnn'nnATC Cuts at: 2588 3333 6133 2588 Size: 745 2800 2594 Fragments arranged by size: 2800 2594 745 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3946 4328 4963 275 Size: 53 83 186 936 2413 382 635 1451 Fragments arranged by size: 2413 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4572 4970 5244 5391 5802 4572 Size: 398 274 147 411 4909 Fragments arranged by size: 4909 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2178 2208 2332 2362 1178 Size: 30 165 30 775 30 124 30 4955 Fragments arranged by size: 4955 775 165 124 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2033 3441 1186 Size: 847 1408 3884 Fragments arranged by size: 3884 1408 847 BseYI C'CCAG_C Cuts at: 5342 5838 5342 Size: 496 5643 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5566 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3589 4350 5378 1540 Size: 275 186 1588 761 1028 2301 Fragments arranged by size: 2301 1588 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4200 4285 5356 4200 Size: 85 1071 4983 Fragments arranged by size: 4983 1071 85 BsmI GAATG_Cn' Cuts at: 2411 3650 3743 2411 Size: 1239 93 4807 Fragments arranged by size: 4807 1239 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3458 4200 4285 5356 1391 Size: 399 1668 742 85 1071 2174 Fragments arranged by size: 2174 1668 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5802 5802 Size: 6139 BspHI T'CATG_A Cuts at: 3127 4028 5758 3127 Size: 901 1730 3508 Fragments arranged by size: 3508 1730 901 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3494 844 Size: 2650 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4026 1394 Size: 429 2203 3507 Fragments arranged by size: 3507 2203 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4634 4808 65 Size: 1183 3386 174 1396 Fragments arranged by size: 3386 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2711 4783 4970 1700 Size: 110 901 2072 187 2869 Fragments arranged by size: 2869 2072 901 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6139 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6139 BssSI C'ACGA_G Cuts at: 4199 5211 4199 Size: 1012 5127 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6139 BstBI TT'CG_AA Cuts at: 4943 4943 Size: 6139 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6139 BstXI CCAn_nnnn'nTGG Cuts at: 2048 2048 Size: 6139 BstYI r'GATC_y Cuts at: 1972 2059 3328 3469 3836 4221 4238 5690 5776 Size: 87 1269 141 367 385 17 1452 86 Cuts at: 5776 5788 6134 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1269 385 367 346 141 87 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4648 Fragments arranged by size: 4648 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2289 487 Size: 683 56 1063 4337 Fragments arranged by size: 4337 1063 683 56 BtsI GCAGTG_nn' Cuts at: 3655 4449 4469 3655 Size: 794 20 5325 Fragments arranged by size: 5325 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6139 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6104 DraI TTT'AAA Cuts at: 3443 3443 Size: 6139 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5146 809 Size: 507 3830 1802 Fragments arranged by size: 3830 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3586 4475 6104 Size: 54 1425 50 275 18 1756 889 1629 Cuts at: 6104 8 Size: 43 Fragments arranged by size: 1756 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3586 1537 Size: 275 1774 4090 Fragments arranged by size: 4090 1774 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2315 2487 4069 1353 Size: 776 186 172 1582 3423 Fragments arranged by size: 3423 1582 776 186 172 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4726 5100 5246 387 Size: 1064 138 3137 374 146 1280 Fragments arranged by size: 3137 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3320 4202 4799 5586 5854 1239 Size: 393 1688 882 597 787 268 1524 Fragments arranged by size: 1688 1524 882 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2144 2144 Size: 6139 EcoRI G'AATT_C Cuts at: 3448 3826 3448 Size: 378 5761 FspI TGC'GCA Cuts at: 4645 4645 Size: 6139 HaeII r_GCGC'y Cuts at: 1678 5286 1678 Size: 3608 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2336 2368 2661 Size: 32 135 32 593 32 139 32 293 Cuts at: 2661 2693 4780 4812 4854 4886 1373 Size: 32 2087 32 42 32 2626 Fragments arranged by size: 2626 2087 593 293 139 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3483 3729 669 Size: 988 281 1545 246 3079 Fragments arranged by size: 3079 1545 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6139 HpaI GTT'AAC Cuts at: 3729 3729 Size: 6139 KpnI G_GTAC'C Cuts at: 3464 3464 Size: 6139 MfeI C'AATT_G Cuts at: 3000 3090 3738 3000 Size: 90 648 5401 Fragments arranged by size: 5401 648 90 MluI A'CGCG_T Cuts at: 6015 6015 Size: 6139 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2196 2583 3151 3425 5253 5437 1305 Size: 891 387 568 274 1828 184 2007 Fragments arranged by size: 2007 1828 891 568 387 274 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4317 Fragments arranged by size: 4317 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2046 2772 3343 4097 4456 4615 518 Size: 648 880 726 571 754 359 159 2042 Fragments arranged by size: 2042 880 754 726 648 571 359 159 MspA1I CmG'CkG Cuts at: 1606 2441 3555 4232 5380 5625 1606 Size: 835 1114 677 1148 245 2120 Fragments arranged by size: 2120 1148 1114 835 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4648 Fragments arranged by size: 4648 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6139 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6139 NotI GC'GGCC_GC Cuts at: 3586 3586 Size: 6139 NspI r_CATG'y Cuts at: 1132 1229 3023 3179 3408 3497 5042 1132 Size: 97 1794 156 229 89 1545 2229 Fragments arranged by size: 2229 1794 1545 229 156 97 89 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6139 PciI A'CATG_T Cuts at: 3175 3404 5038 3175 Size: 229 1634 4276 Fragments arranged by size: 4276 1634 229 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6139 PfoI T'CCnGG_A Cuts at: 2033 2033 Size: 6139 PmeI GTTT'AAAC Cuts at: 3443 3443 Size: 6139 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4208 4240 5721 5753 4208 Size: 32 1481 32 4594 Fragments arranged by size: 4594 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6139 PsiI TTA'TAA Cuts at: 3709 3709 Size: 6139 PstI C_TGCA'G Cuts at: 830 2155 2524 3491 830 Size: 1325 369 967 3478 Fragments arranged by size: 3478 1325 967 369 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6139 PvuII CAG'CTG Cuts at: 2441 2441 Size: 6139 SalI G'TCGA_C Cuts at: 1936 3481 1936 Size: 1545 4594 SapI GCTCTTCn'nnn_ Cuts at: 2129 2129 Size: 6139 SbfI CC_TGCA'GG Cuts at: 3491 3491 Size: 6139 ScaI AGT'ACT Cuts at: 4387 6099 4387 Size: 1712 4427 SfcI C'TryA_G Cuts at: 826 1045 2151 2520 3487 4622 5303 5494 826 Size: 219 1106 369 967 1135 681 191 1471 Fragments arranged by size: 1471 1135 1106 967 681 369 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6139 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6139 SmaI CCC'GGG Cuts at: 1872 3466 1872 Size: 1594 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4243 5144 5406 820 Size: 197 926 2300 901 262 1553 Fragments arranged by size: 2300 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2551 3930 493 Size: 2058 1379 2702 Fragments arranged by size: 2702 2058 1379 SpeI A'CTAG_T Cuts at: 152 152 Size: 6139 SphI G_CATG'C Cuts at: 1132 3497 1132 Size: 2365 3774 SspI AAT'ATT Cuts at: 5 52 4063 5 Size: 47 4011 2081 Fragments arranged by size: 4011 2081 47 StuI AGG'CCT Cuts at: 1517 2652 1517 Size: 1135 5004 StyI C'CwwG_G Cuts at: 513 1065 2004 3578 513 Size: 552 939 1574 3074 Fragments arranged by size: 3074 1574 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2434 4166 4183 4336 4521 4966 325 Size: 1255 854 1732 17 153 185 445 1498 Fragments arranged by size: 1732 1498 1255 854 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3107 4385 6097 Size: 274 80 33 51 1443 1130 1278 1712 Cuts at: 6097 96 Size: 138 Fragments arranged by size: 1712 1443 1278 1130 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2178 2210 2797 2829 3018 3050 6099 6131 2178 Size: 32 587 32 189 32 3049 32 2186 Fragments arranged by size: 3049 2186 587 189 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6139 XbaI T'CTAG_A Cuts at: 3475 3475 Size: 6139 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6139 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6139 XmaI C'CCGG_G Cuts at: 1870 3464 1870 Size: 1594 4545 XmnI GAAnn'nnTTC Cuts at: 4268 4268 Size: 6139 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3947 276 Size: 53 83 186 936 2413 2468 Fragments arranged by size: 2468 2413 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI