(Circular) (Six-base) MAPSORT of: pFN21AB8106.seq Check: 4347 from: 1 to: 5236 pFN21AB8106 5236 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2416 2416 Size: 5236 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3046 278 Size: 53 83 186 936 1510 2468 Fragments arranged by size: 2468 1510 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2036 2208 2579 1937 Size: 99 172 371 4594 Fragments arranged by size: 4594 371 172 99 Acc65I G'GTAC_C Cuts at: 2557 2557 Size: 5236 AclI AA'CG_TT Cuts at: 1563 3363 3736 1563 Size: 1800 373 3063 Fragments arranged by size: 3063 1800 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3299 4683 4951 1632 Size: 1667 1384 268 1917 Fragments arranged by size: 1917 1667 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5039 AflIII A'CryG_T Cuts at: 1203 4135 5112 1203 Size: 2932 977 1327 Fragments arranged by size: 2932 1327 977 AgeI A'CCGG_T Cuts at: 4067 4067 Size: 5236 AhdI GACnn_n'nnGTC Cuts at: 3965 3965 Size: 5236 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5236 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5236 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2351 2383 2351 Size: 32 5204 AlwNI CAG_nnn'CTG Cuts at: 2317 2494 2979 4551 2317 Size: 177 485 1572 3002 Fragments arranged by size: 3002 1572 485 177 ApaLI G'TGCA_C Cuts at: 3293 4449 3293 Size: 1156 4080 ApoI r'AATT_y Cuts at: 1493 2545 2771 2923 5032 1493 Size: 1052 226 152 2109 1697 Fragments arranged by size: 2109 1697 1052 226 152 AseI AT'TA_AT Cuts at: 160 1032 3790 160 Size: 872 2758 1606 Fragments arranged by size: 2758 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5236 AvaI C'yCGr_G Cuts at: 1870 1943 2561 1870 Size: 73 618 4545 Fragments arranged by size: 4545 618 73 BamHI G'GATC_C Cuts at: 2566 2933 2566 Size: 367 4869 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2557 4012 618 Size: 325 217 627 770 1455 1842 Fragments arranged by size: 1842 1455 770 627 325 217 BanII G_rGCy'C Cuts at: 1391 2555 1391 Size: 1164 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4265 BbvCI CC'TCA_GC Cuts at: 2318 2318 Size: 5236 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3427 3461 1903 Size: 34 1490 34 3678 Fragments arranged by size: 3678 1490 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3128 4338 3128 Size: 1210 4026 BclI T'GATC_A Cuts at: 1548 1548 Size: 5236 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3847 136 Size: 107 122 71 631 605 337 1838 1525 Fragments arranged by size: 1838 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2224 5231 2224 Size: 3007 2229 BlpI GC'TnA_GC Cuts at: 2653 2653 Size: 5236 Bme1580I G_kGCm'C Cuts at: 1790 3297 4453 1790 Size: 1507 1156 2573 Fragments arranged by size: 2573 1507 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5236 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2106 2967 3925 Size: 416 53 83 399 24 689 861 958 Cuts at: 3925 442 Size: 1753 Fragments arranged by size: 1753 958 861 689 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5236 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 3896 1239 Size: 1130 1527 2579 Fragments arranged by size: 2579 1527 1130 Bpu10I CC'TnA_GC Cuts at: 2318 2318 Size: 5236 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3361 4226 4524 3361 Size: 865 298 4073 Fragments arranged by size: 4073 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3899 882 Size: 617 2400 2219 Fragments arranged by size: 2400 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3027 493 Size: 711 1823 2702 Fragments arranged by size: 2702 1823 711 BsaBI GATnn'nnATC Cuts at: 2073 5230 2073 Size: 3157 2079 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3043 3425 4060 275 Size: 53 83 186 936 1510 382 635 1451 Fragments arranged by size: 1510 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3669 4067 4341 4488 4899 3669 Size: 398 274 147 411 4006 Fragments arranged by size: 4006 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2351 2381 1178 Size: 30 165 30 948 30 4033 Fragments arranged by size: 4033 948 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5236 BseYI C'CCAG_C Cuts at: 2421 4439 4935 2421 Size: 2018 496 2722 Fragments arranged by size: 2722 2018 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4663 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2686 3447 4475 1540 Size: 275 186 685 761 1028 2301 Fragments arranged by size: 2301 1028 761 685 275 186 BsiHKAI G_wGCw'C Cuts at: 2344 3297 3382 4453 2344 Size: 953 85 1071 3127 Fragments arranged by size: 3127 1071 953 85 BsmI GAATG_Cn' Cuts at: 2747 2840 2747 Size: 93 5143 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2344 2555 3297 3382 4453 1391 Size: 399 554 211 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 554 399 211 85 BspEI T'CCGG_A Cuts at: 4899 4899 Size: 5236 BspHI T'CATG_A Cuts at: 3125 4855 3125 Size: 1730 3506 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2416 2591 844 Size: 1572 175 3489 Fragments arranged by size: 3489 1572 175 BsrBI CCG'CTC Cuts at: 1394 1823 3123 1394 Size: 429 1300 3507 Fragments arranged by size: 3507 1300 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3731 3905 65 Size: 1183 2483 174 1396 Fragments arranged by size: 2483 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3880 4067 1700 Size: 110 2070 187 2869 Fragments arranged by size: 2869 2070 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5236 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5236 BssSI C'ACGA_G Cuts at: 3296 4308 3296 Size: 1012 4224 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5236 BstBI TT'CG_AA Cuts at: 4040 4040 Size: 5236 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5236 BstYI r'GATC_y Cuts at: 1972 2224 2566 2933 3318 3335 4787 4873 4885 Size: 252 342 367 385 17 1452 86 12 Cuts at: 4885 5231 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 385 367 346 342 252 86 17 12 BstZ17I GTA'TAC Cuts at: 2037 2209 2037 Size: 172 5064 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3745 Fragments arranged by size: 3745 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4497 Fragments arranged by size: 4497 683 56 BtsI GCAGTG_nn' Cuts at: 2342 2752 3546 3566 2342 Size: 410 794 20 4012 Fragments arranged by size: 4012 794 410 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5236 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5201 DraI TTT'AAA Cuts at: 2540 2540 Size: 5236 DraIII CAC_nnn'GTG Cuts at: 2440 2440 Size: 5236 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4243 809 Size: 507 2927 1802 Fragments arranged by size: 2927 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2683 3572 5201 Size: 54 1425 50 275 18 853 889 1629 Cuts at: 5201 8 Size: 43 Fragments arranged by size: 1629 1425 889 853 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2683 1537 Size: 275 871 4090 Fragments arranged by size: 4090 871 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2300 3166 1353 Size: 947 866 3423 Fragments arranged by size: 3423 947 866 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3823 4197 4343 387 Size: 1064 138 2234 374 146 1280 Fragments arranged by size: 2234 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 3299 3896 4683 4951 1239 Size: 393 737 930 597 787 268 1524 Fragments arranged by size: 1524 930 787 737 597 393 268 EcoRI G'AATT_C Cuts at: 2545 2923 2545 Size: 378 4858 FspI TGC'GCA Cuts at: 3742 3742 Size: 5236 HaeII r_GCGC'y Cuts at: 1678 2015 4383 1678 Size: 337 2368 2531 Fragments arranged by size: 2531 2368 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 2470 2502 3877 Size: 32 135 32 816 32 50 32 1375 Cuts at: 3877 3909 3951 3983 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1375 816 135 50 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2580 2826 669 Size: 988 281 642 246 3079 Fragments arranged by size: 3079 988 642 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5236 HpaI GTT'AAC Cuts at: 2826 2826 Size: 5236 KpnI G_GTAC'C Cuts at: 2561 2561 Size: 5236 MfeI C'AATT_G Cuts at: 2835 2835 Size: 5236 MluI A'CGCG_T Cuts at: 5112 5112 Size: 5236 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4350 4534 1305 Size: 3045 184 2007 Fragments arranged by size: 3045 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3414 Fragments arranged by size: 3414 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3194 3553 3712 518 Size: 648 2028 359 159 2042 Fragments arranged by size: 2042 2028 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2652 3329 4477 4722 1606 Size: 1046 677 1148 245 2120 Fragments arranged by size: 2120 1148 1046 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3745 Fragments arranged by size: 3745 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5236 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5236 NotI GC'GGCC_GC Cuts at: 2683 2683 Size: 5236 NspI r_CATG'y Cuts at: 1132 1229 2594 4139 1132 Size: 97 1365 1545 2229 Fragments arranged by size: 2229 1545 1365 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5236 PciI A'CATG_T Cuts at: 4135 4135 Size: 5236 PflMI CCAn_nnn'nTGG Cuts at: 1108 2428 1108 Size: 1320 3916 PmeI GTTT'AAAC Cuts at: 2540 2540 Size: 5236 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2351 2383 3305 3337 4818 4850 2351 Size: 32 922 32 1481 32 2737 Fragments arranged by size: 2737 1481 922 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5236 PsiI TTA'TAA Cuts at: 2806 2806 Size: 5236 PstI C_TGCA'G Cuts at: 830 2499 2588 830 Size: 1669 89 3478 Fragments arranged by size: 3478 1669 89 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5236 SalI G'TCGA_C Cuts at: 1936 2578 1936 Size: 642 4594 SbfI CC_TGCA'GG Cuts at: 2588 2588 Size: 5236 ScaI AGT'ACT Cuts at: 2093 3484 5196 2093 Size: 1391 1712 2133 Fragments arranged by size: 2133 1712 1391 SfcI C'TryA_G Cuts at: 826 1045 2495 2584 3719 4400 4591 826 Size: 219 1450 89 1135 681 191 1471 Fragments arranged by size: 1471 1450 1135 681 219 191 89 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5236 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5236 SmaI CCC'GGG Cuts at: 1872 2563 1872 Size: 691 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3340 4241 4503 820 Size: 197 926 1397 901 262 1553 Fragments arranged by size: 1553 1397 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3027 493 Size: 2534 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5236 SphI G_CATG'C Cuts at: 1132 2594 1132 Size: 1462 3774 SspI AAT'ATT Cuts at: 5 52 3160 5 Size: 47 3108 2081 Fragments arranged by size: 3108 2081 47 StuI AGG'CCT Cuts at: 1517 2132 2196 1517 Size: 615 64 4557 Fragments arranged by size: 4557 615 64 StyI C'CwwG_G Cuts at: 513 1065 2004 2675 513 Size: 552 939 671 3074 Fragments arranged by size: 3074 939 671 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3263 3280 3433 3618 4063 325 Size: 1255 1683 17 153 185 445 1498 Fragments arranged by size: 1683 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2091 3482 5194 Size: 274 80 33 51 1443 114 1391 1712 Cuts at: 5194 96 Size: 138 Fragments arranged by size: 1712 1443 1391 274 138 114 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2158 2190 5196 5228 2158 Size: 32 3006 32 2166 Fragments arranged by size: 3006 2166 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2482 1316 Size: 1166 4070 XbaI T'CTAG_A Cuts at: 2572 2572 Size: 5236 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2056 2056 Size: 5236 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5236 XmaI C'CCGG_G Cuts at: 1870 2561 1870 Size: 691 4545 XmnI GAAnn'nnTTC Cuts at: 2147 3365 2147 Size: 1218 4018 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3044 276 Size: 53 83 186 936 1510 2468 Fragments arranged by size: 2468 1510 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI Bsu36I EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI