Restriction map for
pFN21AB8105
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcsI AflII AflIII AgeI
AhdI AluI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspHI
AvaI AviII AvrII BamHI BanII
BbeI BbrPI BbvCI BbvI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Cac8I CelII
CfoI Cfr10I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI Fnu4HI
FseI FspI HaeII HhaI HinP1I
HindIII HpaI HphI ItaI KasI
KpnI KspI MaeII MaeIII MamI
MfeI MluI MslI MspA1I MunI
MvnI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PleI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfaNI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
TseI Tsp45I Tsp509I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcyI 220 GACGCC G[GA]^CG[CT]C
AlwI 60 GATCC GGATC(4/5)
AocI 214 CCTGAGG CC^T[ACGT]AGG
AspI 79 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 171 TGGCCA TGG^CCA
BanI 120 GGTGCC G^G[CT][GA]CC
BbsI 301 GAAGAC GAAGAC(2/6)
BfaI 148 CTAG C^TAG
BmyI 151 GTGCCC G[AGT]GC[ACT]^C
BpuAI 301 GAAGAC GAAGAC(2/6)
BsaHI 220 GACGCC G[GA]^CG[CT]C
BsaI 210 GAGACC GGTCTC(1/5)
BsiYI 9 CCAGTACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 9 CCAGTACAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 210 GAGAC GTCTC(1/5)
BsmFI 85 GTCCC GGGAC(10/14)
Bsp1286I 151 GTGCCC G[AGT]GC[ACT]^C
BspHI 294 TCATGA T^CATGA
BssKI 116 CCAGG ^CC[ACGT]GG
BstNI 116 CCAGG CC^[AT]GG
BstYI 59 AGATCC [GA]^GATC[CT]
Bsu36I 214 CCTGAGG CC^T[ACGT]AGG
DraII 83 GGGTCCC [GA]G^G[ACGT]CC[CT]
DsaI 269 CCATGG C^C[GA][CT]GG
EaeI 171 TGGCCA [CT]^GGCC[GA]
EarI 195 GAAGAG CTCTTC(1/4)
Eco81I 214 CCTGAGG CC^T[ACGT]AGG
EcoO109I 83 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRII 116 CCAGG ^CC[AT]GG
EcoT14I 269 CCATGG C^C[AT][AT]GG
HgaI 220 GACGC GACGC(5/10)
Hin1I 220 GACGCC G[GA]^CG[CT]C
HincII 238 GTCAAC GT[CT]^[GA]AC
HindII 238 GTCAAC GT[CT]^[GA]AC
HinfI 200 GAATC G^A[ACGT]TC
Ksp632I 195 GAAGAG CTCTTC(1/4)
MaeI 148 CTAG C^TAG
MflI 59 AGATCC [GA]^GATC[CT]
MluNI 171 TGGCCA TGG^CCA
MscI 171 TGGCCA TGG^CCA
MseI 187 TTAA T^TAA
MvaI 116 CCAGG CC^[AT]GG
MwoI 106 GCTCCAAAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 269 CCATGG C^CATGG
PflFI 79 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 83 GGGTCCC [GA]G^G[AT]CC[CT]
RcaI 294 TCATGA T^CATGA
ScrFI 116 CCAGG CC^[ACGT]GG
SfcI 324 CTACAG C^T[GA][CT]AG
StyI 269 CCATGG C^C[AT][AT]GG
TfiI 200 GAATC G^A[AT]TC
Tru9I 187 TTAA T^TAA
Tth111I 79 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 260 CCAGTGCTACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 59 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AfaI 12 GTAC GT^AC
144 GTAC
AvaII 21 GGACC G^G[AT]CC
84 GGTCC
BsaJI 116 CCAGGG C^C[ACGT][ACGT]GG
269 CCATGG
BstF5I 25 CATCC GGATG(2/0)
249 CATCC
DdeI 208 CTGAG C^T[ACGT]AG
215 CTGAG
DpnII 60 GATC ^GATC
127 GATC
FokI 25 CATCC GGATG(9/13)
249 CATCC
MboI 60 GATC ^GATC
127 GATC
NdeII 60 GATC ^GATC
127 GATC
NlaIII 270 CATG CATG^
295 CATG
RsaI 12 GTAC GT^AC
144 GTAC
Sau3AI 60 GATC ^GATC
127 GATC
TspRI 261 CAGTG CA[GC]TG[ACGT][ACGT]^
327 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 15 enzymes) #
#########################################
AccIII BsaWI BseAI BspEI BsrI
Cfr13I HaeIII HapII HpaII MboII
MnlI MroI MspI NlaIV Sau96I