(Circular) (Six-base) MAPSORT of: pFN21AB8102.seq Check: 9166 from: 1 to: 5956 pFN21AB8102 5956 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3766 278 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3299 1937 Size: 1362 4594 Acc65I G'GTAC_C Cuts at: 3277 3277 Size: 5956 AclI AA'CG_TT Cuts at: 1563 4083 4456 1563 Size: 2520 373 3063 Fragments arranged by size: 3063 2520 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2539 4019 5403 5671 1632 Size: 907 1480 1384 268 1917 Fragments arranged by size: 1917 1480 1384 907 268 AfeI AGC'GCT Cuts at: 2985 2985 Size: 5956 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5759 AflIII A'CryG_T Cuts at: 1203 2330 4855 5832 1203 Size: 1127 2525 977 1327 Fragments arranged by size: 2525 1327 1127 977 AgeI A'CCGG_T Cuts at: 4787 4787 Size: 5956 AhdI GACnn_n'nnGTC Cuts at: 4685 4685 Size: 5956 AleI CACnn'nnGTG Cuts at: 1166 2222 2756 3036 1166 Size: 1056 534 280 4086 Fragments arranged by size: 4086 1056 534 280 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2732 1184 Size: 1548 4408 AlwNI CAG_nnn'CTG Cuts at: 2241 3699 5271 2241 Size: 1458 1572 2926 Fragments arranged by size: 2926 1572 1458 ApaLI G'TGCA_C Cuts at: 4013 5169 4013 Size: 1156 4800 ApoI r'AATT_y Cuts at: 1493 3265 3491 3643 5752 1493 Size: 1772 226 152 2109 1697 Fragments arranged by size: 2109 1772 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4510 160 Size: 872 3478 1606 Fragments arranged by size: 3478 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 AvaI C'yCGr_G Cuts at: 1870 1943 3281 1870 Size: 73 1338 4545 Fragments arranged by size: 4545 1338 73 BamHI G'GATC_C Cuts at: 3286 3653 3286 Size: 367 5589 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2477 2681 2813 2849 3173 Size: 325 217 627 690 204 132 36 324 Cuts at: 3173 3277 4732 618 Size: 104 1455 1842 Fragments arranged by size: 1842 1455 690 627 325 324 217 204 132 104 36 BanII G_rGCy'C Cuts at: 1391 2402 2654 3275 1391 Size: 1011 252 621 4072 Fragments arranged by size: 4072 1011 621 252 BbeI G_GCGC'C Cuts at: 2817 2817 Size: 5956 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4985 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4147 4181 1903 Size: 34 2210 34 3678 Fragments arranged by size: 3678 2210 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3848 5058 3848 Size: 1210 4746 BclI T'GATC_A Cuts at: 1548 1548 Size: 5956 BfrBI ATG'CAT Cuts at: 2074 2074 Size: 5956 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3111 4567 Size: 107 122 71 631 605 337 1102 1456 Cuts at: 4567 136 Size: 1525 Fragments arranged by size: 1525 1456 1102 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5951 5951 Size: 5956 BlpI GC'TnA_GC Cuts at: 3373 3373 Size: 5956 Bme1580I G_kGCm'C Cuts at: 1790 2646 2852 3178 4017 5173 1790 Size: 856 206 326 839 1156 2573 Fragments arranged by size: 2573 1156 856 839 326 206 BmgBI CAC'GTC Cuts at: 1318 3034 1318 Size: 1716 4240 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2262 3687 4645 Size: 416 53 83 399 24 845 1425 958 Cuts at: 4645 442 Size: 1753 Fragments arranged by size: 1753 1425 958 845 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5956 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2852 4616 1239 Size: 1613 1764 2579 Fragments arranged by size: 2579 1764 1613 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4081 4946 5244 4081 Size: 865 298 4793 Fragments arranged by size: 4793 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2940 4619 882 Size: 617 1441 1679 2219 Fragments arranged by size: 2219 1679 1441 617 BsaAI yAC'GTr Cuts at: 493 1204 3747 493 Size: 711 2543 2702 Fragments arranged by size: 2702 2543 711 BsaBI GATnn'nnATC Cuts at: 3158 5950 3158 Size: 2792 3164 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2814 2940 3763 4145 Size: 53 83 186 936 1281 126 823 382 Cuts at: 4145 4780 275 Size: 635 1451 Fragments arranged by size: 1451 1281 936 823 635 382 186 126 83 53 BsaWI w'CCGG_w Cuts at: 4389 4787 5061 5208 5619 4389 Size: 398 274 147 411 4726 Fragments arranged by size: 4726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 1178 Size: 30 165 30 819 30 4882 Fragments arranged by size: 4882 819 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3041 3137 3244 1186 Size: 1855 96 107 3898 Fragments arranged by size: 3898 1855 107 96 BseYI C'CCAG_C Cuts at: 2621 5159 5655 2621 Size: 2538 496 2922 Fragments arranged by size: 2922 2538 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2822 1163 Size: 573 1086 4297 Fragments arranged by size: 4297 1086 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3406 4167 5195 1540 Size: 275 186 1405 761 1028 2301 Fragments arranged by size: 2301 1405 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2229 2402 3033 4017 4102 5173 2229 Size: 173 631 984 85 1071 3012 Fragments arranged by size: 3012 1071 984 631 173 85 BsmI GAATG_Cn' Cuts at: 2887 3467 3560 2887 Size: 580 93 5283 Fragments arranged by size: 5283 580 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2229 2402 2646 2654 2852 3033 3178 Size: 399 439 173 244 8 198 181 145 Cuts at: 3178 3275 4017 4102 5173 1391 Size: 97 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 439 399 244 198 181 173 145 97 85 8 BspEI T'CCGG_A Cuts at: 5619 5619 Size: 5956 BspHI T'CATG_A Cuts at: 2349 3845 5575 2349 Size: 1496 1730 2730 Fragments arranged by size: 2730 1730 1496 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2211 2702 3311 844 Size: 1367 491 609 3489 Fragments arranged by size: 3489 1367 609 491 BsrBI CCG'CTC Cuts at: 1394 1823 2679 3843 1394 Size: 429 856 1164 3507 Fragments arranged by size: 3507 1164 856 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2097 4451 4625 65 Size: 1183 849 2354 174 1396 Fragments arranged by size: 2354 1396 1183 849 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2895 4600 4787 1700 Size: 110 1085 1705 187 2869 Fragments arranged by size: 2869 1705 1085 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5956 BssSI C'ACGA_G Cuts at: 2273 2317 4016 5028 2273 Size: 44 1699 1012 3201 Fragments arranged by size: 3201 1699 1012 44 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5956 BstBI TT'CG_AA Cuts at: 4760 4760 Size: 5956 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5956 BstXI CCAn_nnnn'nTGG Cuts at: 3166 3166 Size: 5956 BstYI r'GATC_y Cuts at: 1972 3286 3653 4038 4055 5507 5593 5605 5951 Size: 1314 367 385 17 1452 86 12 346 Cuts at: 5951 1972 Size: 1977 Fragments arranged by size: 1977 1452 1314 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3145 3190 487 Size: 683 56 1919 45 3253 Fragments arranged by size: 3253 1919 683 56 45 BtsI GCAGTG_nn' Cuts at: 3472 4266 4286 3472 Size: 794 20 5142 Fragments arranged by size: 5142 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5956 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5921 DraI TTT'AAA Cuts at: 2959 3260 2959 Size: 301 5655 DraIII CAC_nnn'GTG Cuts at: 2576 2824 2576 Size: 248 5708 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4963 809 Size: 507 3647 1802 Fragments arranged by size: 3647 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2703 2796 Size: 54 1425 50 275 18 303 570 93 Cuts at: 2796 3103 3403 4292 5921 8 Size: 307 300 889 1629 43 Fragments arranged by size: 1629 1425 889 570 307 303 300 275 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3403 1537 Size: 275 1591 4090 Fragments arranged by size: 4090 1591 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2284 2870 3221 3886 1353 Size: 931 586 351 665 3423 Fragments arranged by size: 3423 931 665 586 351 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4543 4917 5063 387 Size: 1064 138 2954 374 146 1280 Fragments arranged by size: 2954 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2400 2400 Size: 5956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2539 2852 4019 4616 5403 5671 1239 Size: 393 907 313 1167 597 787 268 1524 Fragments arranged by size: 1524 1167 907 787 597 393 313 268 EcoNI CCTnn'n_nnAGG Cuts at: 2737 2737 Size: 5956 EcoO109I rG'GnC_Cy Cuts at: 2691 2691 Size: 5956 EcoRI G'AATT_C Cuts at: 3265 3643 3265 Size: 378 5578 FspI TGC'GCA Cuts at: 4462 4462 Size: 5956 HaeII r_GCGC'y Cuts at: 1678 2025 2609 2817 2987 5103 1678 Size: 347 584 208 170 2116 2531 Fragments arranged by size: 2531 2116 584 347 208 170 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2192 2224 2286 2318 4597 Size: 32 135 32 620 32 62 32 2279 Cuts at: 4597 4629 4671 4703 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2279 620 135 62 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3300 3546 669 Size: 988 281 1362 246 3079 Fragments arranged by size: 3079 1362 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5956 HpaI GTT'AAC Cuts at: 3546 3546 Size: 5956 KasI G'GCGC_C Cuts at: 2813 2813 Size: 5956 KpnI G_GTAC'C Cuts at: 3281 3281 Size: 5956 MfeI C'AATT_G Cuts at: 3555 3555 Size: 5956 MluI A'CGCG_T Cuts at: 5832 5832 Size: 5956 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2568 5070 5254 1305 Size: 1263 2502 184 2007 Fragments arranged by size: 2502 2007 1263 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2705 3105 10 Size: 54 1425 343 303 570 400 2861 Fragments arranged by size: 2861 1425 570 400 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 2756 3036 3164 3914 4273 4432 Size: 648 1056 534 280 128 750 359 159 Cuts at: 4432 518 Size: 2042 Fragments arranged by size: 2042 1056 750 648 534 359 280 159 128 MspA1I CmG'CkG Cuts at: 1606 2722 2802 3372 4049 5197 5442 1606 Size: 1116 80 570 677 1148 245 2120 Fragments arranged by size: 2120 1148 1116 677 570 245 80 NarI GG'CG_CC Cuts at: 2814 2814 Size: 5956 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4465 Fragments arranged by size: 4465 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5956 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5956 NotI GC'GGCC_GC Cuts at: 3403 3403 Size: 5956 NsiI A_TGCA'T Cuts at: 2076 2076 Size: 5956 NspI r_CATG'y Cuts at: 1132 1229 3151 3314 4859 1132 Size: 97 1922 163 1545 2229 Fragments arranged by size: 2229 1922 1545 163 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5956 PciI A'CATG_T Cuts at: 4855 4855 Size: 5956 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5956 PmeI GTTT'AAAC Cuts at: 3260 3260 Size: 5956 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4025 4057 5538 5570 4025 Size: 32 1481 32 4411 Fragments arranged by size: 4411 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2691 2691 Size: 5956 PshAI GACnn'nnGTC Cuts at: 1613 2268 1613 Size: 655 5301 PsiI TTA'TAA Cuts at: 2364 3526 2364 Size: 1162 4794 PstI C_TGCA'G Cuts at: 830 2721 2991 3308 830 Size: 1891 270 317 3478 Fragments arranged by size: 3478 1891 317 270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5956 PvuII CAG'CTG Cuts at: 2722 2722 Size: 5956 SacI G_AGCT'C Cuts at: 2402 2402 Size: 5956 SalI G'TCGA_C Cuts at: 1936 3298 1936 Size: 1362 4594 SapI GCTCTTCn'nnn_ Cuts at: 2870 2870 Size: 5956 SbfI CC_TGCA'GG Cuts at: 3308 3308 Size: 5956 ScaI AGT'ACT Cuts at: 4204 5916 4204 Size: 1712 4244 SexAI A'CCwGG_T Cuts at: 2820 2820 Size: 5956 SfcI C'TryA_G Cuts at: 826 1045 2717 2987 3304 4439 5120 5311 826 Size: 219 1672 270 317 1135 681 191 1471 Fragments arranged by size: 1672 1471 1135 681 317 270 219 191 SfoI GGC'GCC Cuts at: 2815 2815 Size: 5956 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5956 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5956 SmaI CCC'GGG Cuts at: 1872 3283 1872 Size: 1411 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4060 4961 5223 820 Size: 197 926 2117 901 262 1553 Fragments arranged by size: 2117 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3747 493 Size: 3254 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5956 SphI G_CATG'C Cuts at: 1132 3151 3314 1132 Size: 2019 163 3774 Fragments arranged by size: 3774 2019 163 SspI AAT'ATT Cuts at: 5 52 3880 5 Size: 47 3828 2081 Fragments arranged by size: 3828 2081 47 StuI AGG'CCT Cuts at: 1517 2590 1517 Size: 1073 4883 StyI C'CwwG_G Cuts at: 513 1065 2004 3395 513 Size: 552 939 1391 3074 Fragments arranged by size: 3074 1391 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2495 2635 3158 3983 4000 4153 4338 Size: 1255 915 140 523 825 17 153 185 Cuts at: 4338 4783 325 Size: 445 1498 Fragments arranged by size: 1498 1255 915 825 523 445 185 153 140 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2667 4202 5914 Size: 274 80 33 51 1443 690 1535 1712 Cuts at: 5914 96 Size: 138 Fragments arranged by size: 1712 1535 1443 690 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2370 2402 5916 5948 2370 Size: 32 3514 32 2378 Fragments arranged by size: 3514 2378 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5956 XbaI T'CTAG_A Cuts at: 3292 3292 Size: 5956 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5956 XmaI C'CCGG_G Cuts at: 1870 3281 1870 Size: 1411 4545 XmnI GAAnn'nnTTC Cuts at: 4085 4085 Size: 5956 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3764 276 Size: 53 83 186 936 2230 2468 Fragments arranged by size: 2468 2230 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoRV FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SrfI SwaI XcmI