Restriction map for
pFN21AB8101
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
Acc65I AccII AccIII AclI AflII
AhdI Alw44I AlwI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BclI BfaI
BfrI BglI BlnI BpuAI BsaBI
BseAI BsiEI BsiWI BsmBI BsoBI
BspEI BspHI BspLU11I BsrDI BssHII
BssSI BstAPI BstBI BstEII BstPI
BstUI Bsu36I DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
FbaI FseI FspI HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MseI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tru9I Tth111I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AcsI 235 AAATTC [GA]^AATT[CT]
AflIII 998 ACGTGT A^C[GA][CT]GT
AgeI 34 ACCGGT A^CCGGT
AlwNI 169 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 181 GGGCCC GGGCC^C
ApoI 235 AAATTC [GA]^AATT[CT]
AspHI 126 GAGCTC G[AT]GC[AT]^C
BciVI 905 GTATCC GTATCC(6/5)
BglII 344 AGATCT A^GATCT
BlpI 1333 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1333 GCTGAGC GC^T[ACGT]AGC
BsaAI 997 TACGTG [CT]AC^GT[GA]
BsgI 91 CTGCAC GTGCAG(16/14)
BsiHKAI 126 GAGCTC G[AT]GC[AT]^C
BsmI 46 GAATGC GAATGC(1/-1)
Bsp1407I 632 TGTACA T^GTACA
BspDI 544 ATCGAT AT^CGAT
BspMI 1237 ACCTGC ACCTGC(4/8)
BsrBI 679 CCGCTC CCGCTC(-3/-3)
BsrGI 632 TGTACA T^GTACA
Bst1107I 276 GTATAC GTA^TAC
BstXI 386 CCAATCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 344 AGATCT [GA]^GATC[CT]
BstZ17I 276 GTATAC GTA^TAC
CelII 1333 GCTGAGC GC^T[ACGT]AGC
ClaI 544 ATCGAT AT^CGAT
CpoI 863 CGGACCG CG^G[AT]CCG
DraIII 1021 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1076 CCACGG C^C[GA][CT]GG
EcoT14I 377 CCTTGG C^C[AT][AT]GG
EcoT22I 48 ATGCAT ATGCA^T
HgaI 937 GACGC GACGC(5/10)
MboII 81 GAAGA GAAGA(8/7)
MflI 344 AGATCT [GA]^GATC[CT]
NaeI 1101 GCCGGC GCC^GGC
NgoMIV 1101 GCCGGC G^CCGGC
NsiI 48 ATGCAT ATGCA^T
PflMI 558 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 34 ACCGGT A^CCGGT
RsrII 863 CGGACCG CG^G[AT]CCG
SacI 126 GAGCTC GAGCT^C
SexAI 971 ACCAGGT A^CC[AT]GGT
SmlI 607 CTTGAG C^T[CT][GA]AG
SphI 500 GCATGC GCATG^C
SspBI 632 TGTACA T^GTACA
StuI 374 AGGCCT AGG^CCT
StyI 377 CCTTGG C^C[AT][AT]GG
TfiI 228 GATTC G^A[AT]TC
Tsp509I 236 AATT ^AATT
Van91I 558 CCATGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 344 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AatII 508 GACGTC GACGT^C
1318 GACGTC
AfaI 633 GTAC GT^AC
1044 GTAC
BalI 555 TGGCCA TGG^CCA
882 TGGCCA
BcnI 650 CCCGG CC^[GC]GG
861 CCCGG
BseRI 129 CTCCTC GAGGAG(10/8)
148 GAGGAG
BsrFI 34 ACCGGT [GA]^CCGG[CT]
1101 GCCGGC
BsrI 212 ACTGG ACTGG(1/-1)
553 ACTGG
Cfr10I 34 ACCGGT [GA]^CCGG[CT]
1101 GCCGGC
DraII 180 GGGGCCC [GA]G^G[ACGT]CC[CT]
181 GGGCCCC
EaeI 555 TGGCCA [CT]^GGCC[GA]
882 TGGCCA
EcoO109I 180 GGGGCCC [GA]G^G[ACGT]CC[CT]
181 GGGCCCC
FauI 678 CCCGC CCCGC(4/6)
824 CCCGC
HaeII 413 GGCGCT [GA]GCGC^[CT]
567 GGCGCT
HphI 285 GGTGA GGTGA(8/7)
41 TCACC
MluNI 555 TGGCCA TGG^CCA
882 TGGCCA
MscI 555 TGGCCA TGG^CCA
882 TGGCCA
NciI 650 CCCGG CC^[GC]GG
861 CCCGG
NspI 398 GCATGT [GA]CATG^[CT]
500 GCATGC
PvuII 256 CAGCTG CAG^CTG
835 CAGCTG
RsaI 633 GTAC GT^AC
1044 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AccI AciI AcyI AluI AvaII
BanII BbvI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BsiYI BslI
BsmAI BsmFI Bsp1286I BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HincII
HindII HinfI HpaII ItaI MaeII
MaeIII MboI MnlI MslI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SfaNI TaqI TseI Tsp45I TspRI
TthHB8I XcmI