(Circular) (Six-base) MAPSORT of: pFN21AB8101.seq Check: 4615 from: 1 to: 6046 pFN21AB8101 6046 bp With 182 enzymes: SgfI * February 27, 2009 14:40 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2517 3327 3856 278 Size: 53 83 186 936 981 810 529 2468 Fragments arranged by size: 2468 981 936 810 529 186 83 53 AccI GT'mk_AC Cuts at: 1937 2282 2334 2517 3389 1937 Size: 345 52 183 872 4594 Fragments arranged by size: 4594 872 345 183 52 Acc65I G'GTAC_C Cuts at: 3367 3367 Size: 6046 AclI AA'CG_TT Cuts at: 1563 4173 4546 1563 Size: 2610 373 3063 Fragments arranged by size: 3063 2610 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4109 5493 5761 1632 Size: 2477 1384 268 1917 Fragments arranged by size: 2477 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5849 AflIII A'CryG_T Cuts at: 1203 3003 4945 5922 1203 Size: 1800 1942 977 1327 Fragments arranged by size: 1942 1800 1327 977 AgeI A'CCGG_T Cuts at: 2039 4877 2039 Size: 2838 3208 AhdI GACnn_n'nnGTC Cuts at: 4775 4775 Size: 6046 AleI CACnn'nnGTG Cuts at: 1166 3071 1166 Size: 1905 4141 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6046 AlwNI CAG_nnn'CTG Cuts at: 2179 3789 5361 2179 Size: 1610 1572 2864 Fragments arranged by size: 2864 1610 1572 ApaI G_GGCC'C Cuts at: 2190 2190 Size: 6046 ApaLI G'TGCA_C Cuts at: 4103 5259 4103 Size: 1156 4890 ApoI r'AATT_y Cuts at: 1493 2240 3355 3581 3733 5842 1493 Size: 747 1115 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 747 226 152 AseI AT'TA_AT Cuts at: 160 1032 4600 160 Size: 872 3568 1606 Fragments arranged by size: 3568 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 AvaI C'yCGr_G Cuts at: 1870 1943 3371 1870 Size: 73 1428 4545 Fragments arranged by size: 4545 1428 73 BamHI G'GATC_C Cuts at: 3376 3743 3376 Size: 367 5679 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3367 4822 618 Size: 325 217 627 1580 1455 1842 Fragments arranged by size: 1842 1580 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2135 2190 2672 3129 3171 3365 1391 Size: 744 55 482 457 42 194 4072 Fragments arranged by size: 4072 744 482 457 194 55 42 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5075 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4237 4271 1903 Size: 34 2300 34 3678 Fragments arranged by size: 3678 2300 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2921 3938 5148 2921 Size: 1017 1210 3819 Fragments arranged by size: 3819 1210 1017 BclI T'GATC_A Cuts at: 1548 1548 Size: 6046 BfrBI ATG'CAT Cuts at: 2055 2055 Size: 6046 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4657 136 Size: 107 122 71 631 605 2985 1525 Fragments arranged by size: 2985 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2349 6041 2349 Size: 3692 2354 BlpI GC'TnA_GC Cuts at: 3339 3463 3339 Size: 124 5922 Bme1580I G_kGCm'C Cuts at: 1790 2190 4107 5263 1790 Size: 400 1917 1156 2573 Fragments arranged by size: 2573 1917 1156 400 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6046 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3777 4735 442 Size: 416 53 83 399 24 2360 958 1753 Fragments arranged by size: 2360 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6046 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2152 2152 Size: 6046 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2171 2201 3134 4706 1239 Size: 932 30 933 1572 2579 Fragments arranged by size: 2579 1572 933 932 30 Bpu10I CC'TnA_GC Cuts at: 2915 2915 Size: 6046 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2633 4171 5036 5334 2633 Size: 1538 865 298 3345 Fragments arranged by size: 3345 1538 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2048 2609 3071 4709 882 Size: 617 549 561 462 1638 2219 Fragments arranged by size: 2219 1638 617 561 549 462 BsaAI yAC'GTr Cuts at: 493 1204 3004 3837 493 Size: 711 1800 833 2702 Fragments arranged by size: 2702 1800 833 711 BsaBI GATnn'nnATC Cuts at: 6040 6040 Size: 6046 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2514 2943 3324 3853 Size: 53 83 186 936 981 429 381 529 Cuts at: 3853 4235 4870 275 Size: 382 635 1451 Fragments arranged by size: 1451 981 936 635 529 429 382 381 186 83 53 BsaWI w'CCGG_w Cuts at: 2039 2106 2520 4479 4877 5151 5298 5709 2039 Size: 67 414 1959 398 274 147 411 2376 Fragments arranged by size: 2376 1959 414 411 398 274 147 67 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2144 2174 2933 2963 1178 Size: 30 165 30 741 30 759 30 4261 Fragments arranged by size: 4261 759 741 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2125 2168 1186 Size: 939 43 5064 Fragments arranged by size: 5064 939 43 BseYI C'CCAG_C Cuts at: 2032 2855 5249 5745 2032 Size: 823 2394 496 2333 Fragments arranged by size: 2394 2333 823 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2081 1163 Size: 573 345 5128 Fragments arranged by size: 5128 573 345 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3496 4257 5285 1540 Size: 275 186 1495 761 1028 2301 Fragments arranged by size: 2301 1495 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 4107 4192 5263 2135 Size: 1972 85 1071 2918 Fragments arranged by size: 2918 1972 1071 85 BsmI GAATG_Cn' Cuts at: 2057 3557 3650 2057 Size: 1500 93 4453 Fragments arranged by size: 4453 1500 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2135 2190 2672 3129 3171 3365 4107 Size: 399 345 55 482 457 42 194 742 Cuts at: 4107 4192 5263 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 482 457 399 345 194 85 55 42 BspEI T'CCGG_A Cuts at: 5709 5709 Size: 6046 BspHI T'CATG_A Cuts at: 3935 5665 3935 Size: 1730 4316 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3251 3334 3401 844 Size: 2407 83 67 3489 Fragments arranged by size: 3489 2407 83 67 BsrBI CCG'CTC Cuts at: 1394 1823 2686 3933 1394 Size: 429 863 1247 3507 Fragments arranged by size: 3507 1247 863 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4541 4715 65 Size: 1183 3293 174 1396 Fragments arranged by size: 3293 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2039 3106 4690 4877 1700 Size: 110 229 1067 1584 187 2869 Fragments arranged by size: 2869 1584 1067 229 187 110 BsrGI T'GTAC_A Cuts at: 96 2637 96 Size: 2541 3505 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6046 BssSI C'ACGA_G Cuts at: 4106 5118 4106 Size: 1012 5034 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6046 BstBI TT'CG_AA Cuts at: 4850 4850 Size: 6046 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6046 BstXI CCAn_nnnn'nTGG Cuts at: 2398 2398 Size: 6046 BstYI r'GATC_y Cuts at: 1972 2349 3376 3743 4128 4145 5597 5683 5695 Size: 377 1027 367 385 17 1452 86 12 Cuts at: 5695 6041 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1027 385 377 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2283 2283 Size: 6046 BtgI C'CryG_G Cuts at: 513 1065 2004 3081 513 Size: 552 939 1077 3478 Fragments arranged by size: 3478 1077 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5307 Fragments arranged by size: 5307 683 56 BtsI GCAGTG_nn' Cuts at: 3562 4356 4376 3562 Size: 794 20 5232 Fragments arranged by size: 5232 794 20 ClaI AT'CG_AT Cuts at: 1554 2550 1554 Size: 996 5050 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3214 3249 529 Size: 35 2650 35 3326 Fragments arranged by size: 3326 2650 35 35 DraI TTT'AAA Cuts at: 3350 3350 Size: 6046 DraIII CAC_nnn'GTG Cuts at: 3031 3031 Size: 6046 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5053 809 Size: 507 3737 1802 Fragments arranged by size: 3737 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2560 2887 3493 Size: 54 1425 50 275 18 730 327 606 Cuts at: 3493 4382 6011 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 730 606 327 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3493 1537 Size: 275 1681 4090 Fragments arranged by size: 4090 1681 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3976 1353 Size: 2623 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4633 5007 5153 387 Size: 1064 138 3044 374 146 1280 Fragments arranged by size: 3044 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2133 2133 Size: 6046 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2171 2201 3134 4109 4706 5493 5761 Size: 393 539 30 933 975 597 787 268 Cuts at: 5761 1239 Size: 1524 Fragments arranged by size: 1524 975 933 787 597 539 393 268 30 EcoO109I rG'GnC_Cy Cuts at: 2186 2187 2186 Size: 1 6045 EcoRI G'AATT_C Cuts at: 3355 3733 3355 Size: 378 5668 FspI TGC'GCA Cuts at: 4552 4552 Size: 6046 HaeII r_GCGC'y Cuts at: 1678 2422 2576 5193 1678 Size: 744 154 2617 2531 Fragments arranged by size: 2617 2531 744 154 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2933 2965 4687 4719 4761 Size: 32 135 32 1361 32 1722 32 42 Cuts at: 4761 4793 1373 Size: 32 2626 Fragments arranged by size: 2626 1722 1361 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2227 2488 2623 2983 3390 3636 Size: 988 281 289 261 135 360 407 246 Cuts at: 3636 669 Size: 3079 Fragments arranged by size: 3079 988 407 360 289 281 261 246 135 HindIII A'AGCT_T Cuts at: 748 748 Size: 6046 HpaI GTT'AAC Cuts at: 3636 3636 Size: 6046 KpnI G_GTAC'C Cuts at: 3371 3371 Size: 6046 MfeI C'AATT_G Cuts at: 3645 3645 Size: 6046 MluI A'CGCG_T Cuts at: 5922 5922 Size: 6046 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3102 5160 5344 1305 Size: 1797 2058 184 2007 Fragments arranged by size: 2058 2007 1797 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2562 2889 10 Size: 54 1425 343 730 327 3167 Fragments arranged by size: 3167 1425 730 343 327 54 MslI CAynn'nnrTG Cuts at: 518 1166 2075 2780 3002 3071 4004 4363 4522 Size: 648 909 705 222 69 933 359 159 Cuts at: 4522 518 Size: 2042 Fragments arranged by size: 2042 933 909 705 648 359 222 159 69 MspA1I CmG'CkG Cuts at: 1606 2263 2832 2842 3462 4139 5287 5532 1606 Size: 657 569 10 620 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 657 620 569 245 10 NaeI GCC'GGC Cuts at: 3108 3108 Size: 6046 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4555 Fragments arranged by size: 4555 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6046 NgoMIV G'CCGG_C Cuts at: 3106 3106 Size: 6046 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6046 NotI GC'GGCC_GC Cuts at: 3493 3493 Size: 6046 NsiI A_TGCA'T Cuts at: 2057 2057 Size: 6046 NspI r_CATG'y Cuts at: 1132 1229 2407 2509 3404 4949 1132 Size: 97 1178 102 895 1545 2229 Fragments arranged by size: 2229 1545 1178 895 102 97 PasI CC'CwG_GG Cuts at: 1348 2710 3040 1348 Size: 1362 330 4354 Fragments arranged by size: 4354 1362 330 PciI A'CATG_T Cuts at: 4945 4945 Size: 6046 PflMI CCAn_nnn'nTGG Cuts at: 1108 2569 1108 Size: 1461 4585 PfoI T'CCnGG_A Cuts at: 2865 2865 Size: 6046 PmeI GTTT'AAAC Cuts at: 3350 3350 Size: 6046 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4115 4147 5628 5660 4115 Size: 32 1481 32 4501 Fragments arranged by size: 4501 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6046 PsiI TTA'TAA Cuts at: 3616 3616 Size: 6046 PspOMI G'GGCC_C Cuts at: 2186 2186 Size: 6046 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2102 2134 2102 Size: 32 6014 PstI C_TGCA'G Cuts at: 830 3398 830 Size: 2568 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6046 PvuII CAG'CTG Cuts at: 2263 2842 2263 Size: 579 5467 RsrII CG'GwC_CG Cuts at: 2869 2869 Size: 6046 SacI G_AGCT'C Cuts at: 2135 2135 Size: 6046 SalI G'TCGA_C Cuts at: 1936 3388 1936 Size: 1452 4594 SbfI CC_TGCA'GG Cuts at: 3398 3398 Size: 6046 ScaI AGT'ACT Cuts at: 4294 6006 4294 Size: 1712 4334 SexAI A'CCwGG_T Cuts at: 2976 2976 Size: 6046 SfcI C'TryA_G Cuts at: 826 1045 3394 4529 5210 5401 826 Size: 219 2349 1135 681 191 1471 Fragments arranged by size: 2349 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6046 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6046 SmaI CCC'GGG Cuts at: 1872 3373 1872 Size: 1501 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2612 4150 5051 5313 820 Size: 197 926 669 1538 901 262 1553 Fragments arranged by size: 1553 1538 926 901 669 262 197 SnaBI TAC'GTA Cuts at: 493 3837 493 Size: 3344 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6046 SphI G_CATG'C Cuts at: 1132 2509 3404 1132 Size: 1377 895 3774 Fragments arranged by size: 3774 1377 895 SspI AAT'ATT Cuts at: 5 52 3970 5 Size: 47 3918 2081 Fragments arranged by size: 3918 2081 47 StuI AGG'CCT Cuts at: 1517 2381 1517 Size: 864 5182 StyI C'CwwG_G Cuts at: 513 1065 2004 2382 3485 513 Size: 552 939 378 1103 3074 Fragments arranged by size: 3074 1103 939 552 378 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4073 4090 4243 4428 4873 325 Size: 1255 2493 17 153 185 445 1498 Fragments arranged by size: 2493 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2637 3048 4292 Size: 274 80 33 51 1443 660 411 1244 Cuts at: 4292 6004 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1244 660 411 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6006 6038 6006 Size: 32 6014 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6046 XbaI T'CTAG_A Cuts at: 3382 3382 Size: 6046 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2146 2245 3070 2146 Size: 99 825 5122 Fragments arranged by size: 5122 825 99 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6046 XmaI C'CCGG_G Cuts at: 1870 3371 1870 Size: 1501 4545 XmnI GAAnn'nnTTC Cuts at: 4175 4175 Size: 6046 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2515 3325 3854 276 Size: 53 83 186 936 981 810 529 2468 Fragments arranged by size: 2468 981 936 810 529 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NarI NruI PacI PmlI PpuMI PspXI SacII SanDI SapI SfiI SfoI SrfI SwaI