Restriction map for
pFN21AB8101
(Linear) (Six-base) MAPSORT of: KIBB8101.seq Check: 4412 from: 1 to: 1341
KIBB8101.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AatII G_ACGT'C
Cuts at: 0 512 1322 1341
Size: 512 810 19
Fragments arranged by size:
810 512 19
AccI GT'mk_AC
Cuts at: 0 277 329 512 1341
Size: 277 52 183 829
Fragments arranged by size:
829 277 183 52
AflIII A'CryG_T
Cuts at: 0 998 1341
Size: 998 343
AgeI A'CCGG_T
Cuts at: 0 34 1341
Size: 34 1307
AleI CACnn'nnGTG
Cuts at: 0 1066 1341
Size: 1066 275
AlwNI CAG_nnn'CTG
Cuts at: 0 174 1341
Size: 174 1167
ApaI G_GGCC'C
Cuts at: 0 185 1341
Size: 185 1156
ApoI r'AATT_y
Cuts at: 0 235 1341
Size: 235 1106
BanII G_rGCy'C
Cuts at: 0 130 185 667 1124 1166 1341
Size: 130 55 482 457 42 175
Fragments arranged by size:
482 457 175 130 55 42
BciVI GTATCCnnnnn_n'
Cuts at: 0 916 1341
Size: 916 425
BfrBI ATG'CAT
Cuts at: 0 50 1341
Size: 50 1291
BglII A'GATC_T
Cuts at: 0 344 1341
Size: 344 997
BlpI GC'TnA_GC
Cuts at: 0 1334 1341
Size: 1334 7
Bme1580I G_kGCm'C
Cuts at: 0 185 1341
Size: 185 1156
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 147 1341
Size: 147 1194
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 196 1129 1341
Size: 166 30 933 212
Fragments arranged by size:
933 212 166 30
Bpu10I CC'TnA_GC
Cuts at: 0 910 1341
Size: 910 431
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 628 1341
Size: 628 713
BsaI GGTCTCn'nnnn_
Cuts at: 0 43 604 1066 1341
Size: 43 561 462 275
Fragments arranged by size:
561 462 275 43
BsaAI yAC'GTr
Cuts at: 0 999 1341
Size: 999 342
BsaHI Gr'CG_yC
Cuts at: 0 509 938 1319 1341
Size: 509 429 381 22
Fragments arranged by size:
509 429 381 22
BsaWI w'CCGG_w
Cuts at: 0 34 101 515 1341
Size: 34 67 414 826
Fragments arranged by size:
826 414 67 34
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 139 169 928 958 1341
Size: 139 30 759 30 383
Fragments arranged by size:
759 383 139 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 120 163 1341
Size: 120 43 1178
Fragments arranged by size:
1178 120 43
BseYI C'CCAG_C
Cuts at: 0 27 850 1341
Size: 27 823 491
Fragments arranged by size:
823 491 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1341
Size: 76 1265
BsiHKAI G_wGCw'C
Cuts at: 0 130 1341
Size: 130 1211
BsmI GAATG_Cn'
Cuts at: 0 52 1341
Size: 52 1289
Bsp1286I G_dGCh'C
Cuts at: 0 130 185 667 1124 1166 1341
Size: 130 55 482 457 42 175
Fragments arranged by size:
482 457 175 130 55 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1246 1341
Size: 1246 95
BsrBI CCG'CTC
Cuts at: 0 681 1341
Size: 681 660
BsrFI r'CCGG_y
Cuts at: 0 34 1101 1341
Size: 34 1067 240
Fragments arranged by size:
1067 240 34
BsrGI T'GTAC_A
Cuts at: 0 632 1341
Size: 632 709
BstXI CCAn_nnnn'nTGG
Cuts at: 0 393 1341
Size: 393 948
BstYI r'GATC_y
Cuts at: 0 344 1341
Size: 344 997
BstZ17I GTA'TAC
Cuts at: 0 278 1341
Size: 278 1063
BtgI C'CryG_G
Cuts at: 0 1076 1341
Size: 1076 265
ClaI AT'CG_AT
Cuts at: 0 545 1341
Size: 545 796
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1209 1244 1341
Size: 1209 35 97
Fragments arranged by size:
1209 97 35
DraIII CAC_nnn'GTG
Cuts at: 0 1026 1341
Size: 1026 315
EaeI y'GGCC_r
Cuts at: 0 555 882 1341
Size: 555 327 459
Fragments arranged by size:
555 459 327
EcoICRI GAG'CTC
Cuts at: 0 128 1341
Size: 128 1213
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 196 1129 1341
Size: 166 30 933 212
Fragments arranged by size:
933 212 166 30
EcoO109I rG'GnC_Cy
Cuts at: 0 181 182 1341
Size: 181 1 1159
Fragments arranged by size:
1159 181 1
HaeII r_GCGC'y
Cuts at: 0 417 571 1341
Size: 417 154 770
Fragments arranged by size:
770 417 154
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 928 960 1341
Size: 928 32 381
Fragments arranged by size:
928 381 32
HincII GTy'rAC
Cuts at: 0 222 483 618 978 1341
Size: 222 261 135 360 363
Fragments arranged by size:
363 360 261 222 135
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1097 1341
Size: 1097 244
MscI TGG'CCA
Cuts at: 0 557 884 1341
Size: 557 327 457
Fragments arranged by size:
557 457 327
MslI CAynn'nnrTG
Cuts at: 0 70 775 997 1066 1341
Size: 70 705 222 69 275
Fragments arranged by size:
705 275 222 70 69
MspA1I CmG'CkG
Cuts at: 0 258 827 837 1341
Size: 258 569 10 504
Fragments arranged by size:
569 504 258 10
NaeI GCC'GGC
Cuts at: 0 1103 1341
Size: 1103 238
NgoMIV G'CCGG_C
Cuts at: 0 1101 1341
Size: 1101 240
NsiI A_TGCA'T
Cuts at: 0 52 1341
Size: 52 1289
NspI r_CATG'y
Cuts at: 0 402 504 1341
Size: 402 102 837
Fragments arranged by size:
837 402 102
PasI CC'CwG_GG
Cuts at: 0 705 1035 1341
Size: 705 330 306
Fragments arranged by size:
705 330 306
PflMI CCAn_nnn'nTGG
Cuts at: 0 564 1341
Size: 564 777
PfoI T'CCnGG_A
Cuts at: 0 860 1341
Size: 860 481
PspOMI G'GGCC_C
Cuts at: 0 181 1341
Size: 181 1160
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 97 129 1341
Size: 97 32 1212
Fragments arranged by size:
1212 97 32
PvuII CAG'CTG
Cuts at: 0 258 837 1341
Size: 258 579 504
Fragments arranged by size:
579 504 258
RsrII CG'GwC_CG
Cuts at: 0 864 1341
Size: 864 477
SacI G_AGCT'C
Cuts at: 0 130 1341
Size: 130 1211
SexAI A'CCwGG_T
Cuts at: 0 971 1341
Size: 971 370
SmlI C'TyrA_G
Cuts at: 0 607 1341
Size: 607 734
SphI G_CATG'C
Cuts at: 0 504 1341
Size: 504 837
StuI AGG'CCT
Cuts at: 0 376 1341
Size: 376 965
StyI C'CwwG_G
Cuts at: 0 377 1341
Size: 377 964
TatI w'GTAC_w
Cuts at: 0 632 1043 1341
Size: 632 411 298
Fragments arranged by size:
632 411 298
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 141 240 1065 1341
Size: 141 99 825 276
Fragments arranged by size:
825 276 141 99
ZraI GAC'GTC
Cuts at: 0 510 1320 1341
Size: 510 810 21
Fragments arranged by size:
810 510 21
Enzymes that do cut:
AatII AccI AflIII AgeI AleI AlwNI ApaI ApoI
BanII BciVI BfrBI BglII BlpI Bme1580I BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI
BsrGI BstXI BstYI BstZ17I BtgI ClaI CspCI DraIII
EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I HincII MmeI
MscI MslI MspA1I NaeI NgoMIV NsiI NspI PasI
PflMI PfoI PspOMI PsrI PvuII RsrII SacI SexAI
SmlI SphI StuI StyI TatI XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AcuI AfeI AflII AhdI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BcgI BclI BglI
BmgBI BmrI BmtI BsaBI BsiEI BsiWI BsmBI BspEI
BspHI BsrDI BssHII BssSI BstAPI BstBI BstEII Bsu36I
BtgZI BtsI DraI DrdI EagI EarI EciI EcoNI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
KasI KpnI MfeI MluI NarI NcoI NdeI NheI
NotI NruI PacI PciI PmeI PmlI PpiI PpuMI
PshAI PsiI PspXI PstI PvuI SacII SalI SanDI
SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SrfI SspI SwaI TaqII TstI
Tth111I XbaI XhoI XmaI XmnI