(Circular) (Six-base) MAPSORT of: pFN21AB7795.seq Check: 2989 from: 1 to: 6088 pFN21AB7795 6088 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3898 278 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3431 1937 Size: 1494 4594 Acc65I G'GTAC_C Cuts at: 2264 3409 2264 Size: 1145 4943 AclI AA'CG_TT Cuts at: 1563 4215 4588 1563 Size: 2652 373 3063 Fragments arranged by size: 3063 2652 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2136 2279 2352 2415 3395 4151 5535 5803 Size: 504 143 73 63 980 756 1384 268 Cuts at: 5803 1632 Size: 1917 Fragments arranged by size: 1917 1384 980 756 504 268 143 73 63 AfeI AGC'GCT Cuts at: 2849 2849 Size: 6088 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5891 AflIII A'CryG_T Cuts at: 1203 3224 4987 5964 1203 Size: 2021 1763 977 1327 Fragments arranged by size: 2021 1763 1327 977 AgeI A'CCGG_T Cuts at: 2551 4919 2551 Size: 2368 3720 AhdI GACnn_n'nnGTC Cuts at: 4817 4817 Size: 6088 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6088 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6088 AlwNI CAG_nnn'CTG Cuts at: 2141 2534 3831 5403 2141 Size: 393 1297 1572 2826 Fragments arranged by size: 2826 1572 1297 393 ApaI G_GGCC'C Cuts at: 3059 3059 Size: 6088 ApaLI G'TGCA_C Cuts at: 4145 5301 4145 Size: 1156 4932 ApoI r'AATT_y Cuts at: 1493 3397 3623 3775 5884 1493 Size: 1904 226 152 2109 1697 Fragments arranged by size: 2109 1904 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4642 160 Size: 872 3610 1606 Fragments arranged by size: 3610 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 AvaI C'yCGr_G Cuts at: 1870 1943 2161 2750 2831 3142 3413 1870 Size: 73 218 589 81 311 271 4545 Fragments arranged by size: 4545 589 311 271 218 81 73 BamHI G'GATC_C Cuts at: 3418 3785 3418 Size: 367 5721 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2264 2745 2922 3099 3409 Size: 325 217 627 477 481 177 177 310 Cuts at: 3409 4864 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 481 477 325 310 217 177 177 BanII G_rGCy'C Cuts at: 1391 2074 2274 2564 2579 2821 2961 3059 3407 Size: 683 200 290 15 242 140 98 348 Cuts at: 3407 1391 Size: 4072 Fragments arranged by size: 4072 683 348 290 242 200 140 98 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2142 928 Size: 971 243 4874 Fragments arranged by size: 4874 971 243 BbvCI CC'TCA_GC Cuts at: 2461 2648 2461 Size: 187 5901 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2785 2819 3139 3173 4279 4313 1903 Size: 34 848 34 320 34 1106 34 3678 Fragments arranged by size: 3678 1106 848 320 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3980 5190 3980 Size: 1210 4878 BclI T'GATC_A Cuts at: 1548 1548 Size: 6088 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2228 2688 4699 Size: 107 122 71 631 605 556 460 2011 Cuts at: 4699 136 Size: 1525 Fragments arranged by size: 2011 1525 631 605 556 460 122 107 71 BglII A'GATC_T Cuts at: 6083 6083 Size: 6088 BlpI GC'TnA_GC Cuts at: 3086 3505 3086 Size: 419 5669 Bme1580I G_kGCm'C Cuts at: 1790 2915 3059 4149 5305 1790 Size: 1125 144 1090 1156 2573 Fragments arranged by size: 2573 1156 1125 1090 144 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6088 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3819 4777 442 Size: 416 53 83 399 24 2402 958 1753 Fragments arranged by size: 2402 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6088 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2869 2869 Size: 6088 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2492 4748 1239 Size: 818 435 2256 2579 Fragments arranged by size: 2579 2256 818 435 Bpu10I CC'TnA_GC Cuts at: 2461 2648 2461 Size: 187 5901 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4213 5078 5376 4213 Size: 865 298 4925 Fragments arranged by size: 4925 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4751 882 Size: 617 3252 2219 Fragments arranged by size: 3252 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 3879 493 Size: 711 2021 654 2702 Fragments arranged by size: 2702 2021 711 654 BsaBI GATnn'nnATC Cuts at: 6082 6082 Size: 6088 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3895 4277 4912 275 Size: 53 83 186 936 2362 382 635 1451 Fragments arranged by size: 2362 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2551 4521 4919 5193 5340 5751 2551 Size: 1970 398 274 147 411 2888 Fragments arranged by size: 2888 1970 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2375 2400 2405 2430 1178 Size: 30 165 30 972 25 5 25 4836 Fragments arranged by size: 4836 972 165 30 30 25 25 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2233 1186 Size: 1047 5041 BseYI C'CCAG_C Cuts at: 2411 2626 2711 5291 5787 2411 Size: 215 85 2580 496 2712 Fragments arranged by size: 2712 2580 496 215 85 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5515 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3247 3538 4299 5327 1540 Size: 275 186 1246 291 761 1028 2301 Fragments arranged by size: 2301 1246 1028 761 291 275 186 BsiHKAI G_wGCw'C Cuts at: 2564 2757 3165 4149 4234 5305 2564 Size: 193 408 984 85 1071 3347 Fragments arranged by size: 3347 1071 984 408 193 85 BsmI GAATG_Cn' Cuts at: 3131 3599 3692 3131 Size: 468 93 5527 Fragments arranged by size: 5527 468 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2436 2436 Size: 6088 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2074 2274 2564 2579 2757 2821 2915 Size: 399 284 200 290 15 178 64 94 Cuts at: 2915 2961 3059 3165 3407 4149 4234 5305 1391 Size: 46 98 106 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 290 284 242 200 178 106 98 94 85 64 46 15 BspEI T'CCGG_A Cuts at: 5751 5751 Size: 6088 BspHI T'CATG_A Cuts at: 3977 5707 3977 Size: 1730 4358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 844 Size: 2599 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3316 3975 1394 Size: 429 1493 659 3507 Fragments arranged by size: 3507 1493 659 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4583 4757 65 Size: 1183 3335 174 1396 Fragments arranged by size: 3335 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2551 3242 3344 4732 4919 1700 Size: 110 741 691 102 1388 187 2869 Fragments arranged by size: 2869 1388 741 691 187 110 102 BsrGI T'GTAC_A Cuts at: 96 2181 96 Size: 2085 4003 BssHII G'CGCG_C Cuts at: 1925 3090 3340 1925 Size: 1165 250 4673 Fragments arranged by size: 4673 1165 250 BssSI C'ACGA_G Cuts at: 2981 3164 4148 5160 2981 Size: 183 984 1012 3909 Fragments arranged by size: 3909 1012 984 183 BstAPI GCAn_nnn'nTGC Cuts at: 794 2193 3126 3303 794 Size: 1399 933 177 3579 Fragments arranged by size: 3579 1399 933 177 BstBI TT'CG_AA Cuts at: 4892 4892 Size: 6088 BstEII G'GTnAC_C Cuts at: 1184 2899 1184 Size: 1715 4373 BstXI CCAn_nnnn'nTGG Cuts at: 2785 2785 Size: 6088 BstYI r'GATC_y Cuts at: 1972 3418 3785 4170 4187 5639 5725 5737 6083 Size: 1446 367 385 17 1452 86 12 346 Cuts at: 6083 1972 Size: 1977 Fragments arranged by size: 1977 1452 1446 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5349 Fragments arranged by size: 5349 683 56 BtsI GCAGTG_nn' Cuts at: 3604 4398 4418 3604 Size: 794 20 5274 Fragments arranged by size: 5274 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6088 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6053 DraI TTT'AAA Cuts at: 3392 3392 Size: 6088 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3113 5095 809 Size: 507 1797 1982 1802 Fragments arranged by size: 1982 1802 1797 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 3244 3535 Size: 54 1425 50 275 18 289 1125 291 Cuts at: 3535 4424 6053 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1125 889 291 289 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3244 3535 1537 Size: 275 1432 291 4090 Fragments arranged by size: 4090 1432 291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2251 3386 4018 1353 Size: 898 1135 632 3423 Fragments arranged by size: 3423 1135 898 632 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4675 5049 5195 387 Size: 1064 138 3086 374 146 1280 Fragments arranged by size: 3086 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2562 2562 Size: 6088 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2136 2279 2352 2415 2492 3395 Size: 393 425 79 143 73 63 77 903 Cuts at: 3395 4151 4748 5535 5803 1239 Size: 756 597 787 268 1524 Fragments arranged by size: 1524 903 787 756 597 425 393 268 143 79 77 73 63 EcoNI CCTnn'n_nnAGG Cuts at: 2444 2444 Size: 6088 EcoO109I rG'GnC_Cy Cuts at: 2011 3055 3056 3327 2011 Size: 1044 1 271 4772 Fragments arranged by size: 4772 1044 271 1 EcoRI G'AATT_C Cuts at: 3397 3775 3397 Size: 378 5710 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3346 3346 Size: 6088 FspI TGC'GCA Cuts at: 4594 4594 Size: 6088 HaeII r_GCGC'y Cuts at: 1678 2086 2851 3086 5235 1678 Size: 408 765 235 2149 2531 Fragments arranged by size: 2531 2149 765 408 235 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2400 2407 2432 4729 Size: 32 135 32 803 25 7 25 2297 Cuts at: 4729 4761 4803 4835 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2297 803 135 42 32 32 32 32 25 25 7 HincII GTy'rAC Cuts at: 669 1657 1938 3432 3678 669 Size: 988 281 1494 246 3079 Fragments arranged by size: 3079 1494 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6088 HpaI GTT'AAC Cuts at: 3678 3678 Size: 6088 KpnI G_GTAC'C Cuts at: 2268 3413 2268 Size: 1145 4943 MfeI C'AATT_G Cuts at: 3687 3687 Size: 6088 MluI A'CGCG_T Cuts at: 5964 5964 Size: 6088 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2667 2911 5202 5386 1305 Size: 1362 244 2291 184 2007 Fragments arranged by size: 2291 2007 1362 244 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2121 10 Size: 54 1425 343 289 3977 Fragments arranged by size: 3977 1425 343 289 54 MslI CAynn'nnrTG Cuts at: 518 1166 4046 4405 4564 518 Size: 648 2880 359 159 2042 Fragments arranged by size: 2880 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2253 3025 3249 3504 4181 5329 5574 1606 Size: 647 772 224 255 677 1148 245 2120 Fragments arranged by size: 2120 1148 772 677 647 255 245 224 NaeI GCC'GGC Cuts at: 3346 3346 Size: 6088 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4597 Fragments arranged by size: 4597 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6088 NgoMIV G'CCGG_C Cuts at: 3344 3344 Size: 6088 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6088 NotI GC'GGCC_GC Cuts at: 3535 3535 Size: 6088 NspI r_CATG'y Cuts at: 1132 1229 2059 3297 3446 4991 1132 Size: 97 830 1238 149 1545 2229 Fragments arranged by size: 2229 1545 1238 830 149 97 PasI CC'CwG_GG Cuts at: 1348 2785 3262 1348 Size: 1437 477 4174 Fragments arranged by size: 4174 1437 477 PciI A'CATG_T Cuts at: 4987 4987 Size: 6088 PflMI CCAn_nnn'nTGG Cuts at: 1108 2117 2534 2864 1108 Size: 1009 417 330 4332 Fragments arranged by size: 4332 1009 417 330 PfoI T'CCnGG_A Cuts at: 3063 3333 3063 Size: 270 5818 PmeI GTTT'AAAC Cuts at: 3392 3392 Size: 6088 PmlI CAC'GTG Cuts at: 3225 3225 Size: 6088 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4157 4189 5670 5702 4157 Size: 32 1481 32 4543 Fragments arranged by size: 4543 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2011 3327 2011 Size: 1316 4772 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6088 PsiI TTA'TAA Cuts at: 3658 3658 Size: 6088 PspOMI G'GGCC_C Cuts at: 3055 3055 Size: 6088 PstI C_TGCA'G Cuts at: 830 2221 2640 3024 3122 3440 830 Size: 1391 419 384 98 318 3478 Fragments arranged by size: 3478 1391 419 384 318 98 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6088 PvuII CAG'CTG Cuts at: 3025 3025 Size: 6088 SacI G_AGCT'C Cuts at: 2564 2564 Size: 6088 SalI G'TCGA_C Cuts at: 1936 3430 1936 Size: 1494 4594 SapI GCTCTTCn'nnn_ Cuts at: 3386 3386 Size: 6088 SbfI CC_TGCA'GG Cuts at: 3440 3440 Size: 6088 ScaI AGT'ACT Cuts at: 4336 6048 4336 Size: 1712 4376 SfcI C'TryA_G Cuts at: 826 1045 2217 2636 3020 3118 3436 4571 5252 Size: 219 1172 419 384 98 318 1135 681 Cuts at: 5252 5443 826 Size: 191 1471 Fragments arranged by size: 1471 1172 1135 681 419 384 318 219 191 98 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6088 SgrAI Cr'CCGG_yG Cuts at: 1700 2551 1700 Size: 851 5237 SmaI CCC'GGG Cuts at: 1872 3415 1872 Size: 1543 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4192 5093 5355 820 Size: 197 926 2249 901 262 1553 Fragments arranged by size: 2249 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3879 493 Size: 3386 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6088 SphI G_CATG'C Cuts at: 1132 3446 1132 Size: 2314 3774 SspI AAT'ATT Cuts at: 5 52 4012 5 Size: 47 3960 2081 Fragments arranged by size: 3960 2081 47 StuI AGG'CCT Cuts at: 1517 2682 1517 Size: 1165 4923 StyI C'CwwG_G Cuts at: 513 1065 2004 2060 2907 3321 3527 513 Size: 552 939 56 847 414 206 3074 Fragments arranged by size: 3074 939 847 552 414 206 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2193 2684 2940 4115 4132 4285 4470 Size: 1255 613 491 256 1175 17 153 185 Cuts at: 4470 4915 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1175 613 491 445 256 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2181 4334 6046 Size: 274 80 33 51 1443 204 2153 1712 Cuts at: 6046 96 Size: 138 Fragments arranged by size: 2153 1712 1443 274 204 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6048 6080 6048 Size: 32 6056 Tth111I GACn'n_nGTC Cuts at: 1316 2137 1316 Size: 821 5267 XbaI T'CTAG_A Cuts at: 3424 3424 Size: 6088 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2257 2473 2011 Size: 246 216 5626 Fragments arranged by size: 5626 246 216 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6088 XmaI C'CCGG_G Cuts at: 1870 3413 1870 Size: 1543 4545 XmnI GAAnn'nnTTC Cuts at: 3191 3380 4217 3191 Size: 189 837 5062 Fragments arranged by size: 5062 837 189 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3896 276 Size: 53 83 186 936 2362 2468 Fragments arranged by size: 2468 2362 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI