(Circular) (Six-base) MAPSORT of: pFN21AB7789.seq Check: 7100 from: 1 to: 6079 pFN21AB7789 6079 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3889 278 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2316 3422 1937 Size: 379 1106 4594 Fragments arranged by size: 4594 1106 379 Acc65I G'GTAC_C Cuts at: 3400 3400 Size: 6079 AclI AA'CG_TT Cuts at: 1563 4206 4579 1563 Size: 2643 373 3063 Fragments arranged by size: 3063 2643 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2031 2244 4142 5526 5794 1632 Size: 399 213 1898 1384 268 1917 Fragments arranged by size: 1917 1898 1384 399 268 213 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5882 AflIII A'CryG_T Cuts at: 1203 4978 5955 1203 Size: 3775 977 1327 Fragments arranged by size: 3775 1327 977 AgeI A'CCGG_T Cuts at: 4910 4910 Size: 6079 AhdI GACnn_n'nnGTC Cuts at: 4808 4808 Size: 6079 AleI CACnn'nnGTG Cuts at: 1166 2528 1166 Size: 1362 4717 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6079 AlwNI CAG_nnn'CTG Cuts at: 2791 3096 3822 5394 2791 Size: 305 726 1572 3476 Fragments arranged by size: 3476 1572 726 305 ApaI G_GGCC'C Cuts at: 2079 2893 2079 Size: 814 5265 ApaLI G'TGCA_C Cuts at: 4136 5292 4136 Size: 1156 4923 ApoI r'AATT_y Cuts at: 1493 2267 3388 3614 3766 5875 1493 Size: 774 1121 226 152 2109 1697 Fragments arranged by size: 2109 1697 1121 774 226 152 AseI AT'TA_AT Cuts at: 160 1032 4633 160 Size: 872 3601 1606 Fragments arranged by size: 3601 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 AvaI C'yCGr_G Cuts at: 1870 1943 2072 2078 2116 2238 2711 3404 1870 Size: 73 129 6 38 122 473 693 4545 Fragments arranged by size: 4545 693 473 129 122 73 38 6 BamHI G'GATC_C Cuts at: 3409 3776 3409 Size: 367 5712 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3340 3400 4855 618 Size: 325 217 627 1553 60 1455 1842 Fragments arranged by size: 1842 1553 1455 627 325 217 60 BanII G_rGCy'C Cuts at: 1391 2073 2079 2394 2893 3021 3398 1391 Size: 682 6 315 499 128 377 4072 Fragments arranged by size: 4072 682 499 377 315 128 6 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2471 2607 928 Size: 971 572 136 4400 Fragments arranged by size: 4400 971 572 136 BbvCI CC'TCA_GC Cuts at: 2097 3158 2097 Size: 1061 5018 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2555 2589 4270 4304 1903 Size: 34 618 34 1681 34 3678 Fragments arranged by size: 3678 1681 618 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3971 5181 3971 Size: 1210 4869 BclI T'GATC_A Cuts at: 1548 1548 Size: 6079 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 4690 136 Size: 107 122 71 631 605 337 2681 1525 Fragments arranged by size: 2681 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 2721 6074 2721 Size: 3353 2726 BlpI GC'TnA_GC Cuts at: 3080 3496 3080 Size: 416 5663 Bme1580I G_kGCm'C Cuts at: 1790 2079 2893 3140 3292 3343 4140 5296 1790 Size: 289 814 247 152 51 797 1156 2573 Fragments arranged by size: 2573 1156 814 797 289 247 152 51 BmgBI CAC'GTC Cuts at: 1318 2842 1318 Size: 1524 4555 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3286 3810 4768 Size: 416 53 83 399 24 1869 524 958 Cuts at: 4768 442 Size: 1753 Fragments arranged by size: 1869 1753 958 524 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6079 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3050 3134 4739 1239 Size: 1811 84 1605 2579 Fragments arranged by size: 2579 1811 1605 84 Bpu10I CC'TnA_GC Cuts at: 2097 2384 2406 3158 2097 Size: 287 22 752 5018 Fragments arranged by size: 5018 752 287 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2630 3317 4204 5069 5367 2630 Size: 687 887 865 298 3342 Fragments arranged by size: 3342 887 865 687 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4742 882 Size: 617 3243 2219 Fragments arranged by size: 3243 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2284 3244 3870 493 Size: 711 1080 960 626 2702 Fragments arranged by size: 2702 1080 960 711 626 BsaBI GATnn'nnATC Cuts at: 2552 6073 2552 Size: 3521 2558 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3886 4268 4903 275 Size: 53 83 186 936 2353 382 635 1451 Fragments arranged by size: 2353 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2200 4512 4910 5184 5331 5742 2200 Size: 2312 398 274 147 411 2537 Fragments arranged by size: 2537 2312 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2909 2939 1178 Size: 30 165 30 1506 30 4318 Fragments arranged by size: 4318 1506 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3287 1186 Size: 2101 3978 BseYI C'CCAG_C Cuts at: 5282 5778 5282 Size: 496 5583 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2480 1163 Size: 573 744 4762 Fragments arranged by size: 4762 744 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2094 2201 3529 4290 5318 1540 Size: 275 186 93 107 1328 761 1028 2301 Fragments arranged by size: 2301 1328 1028 761 275 186 107 93 BsiHKAI G_wGCw'C Cuts at: 2394 3021 3038 3279 4140 4225 5296 2394 Size: 627 17 241 861 85 1071 3177 Fragments arranged by size: 3177 1071 861 627 241 85 17 BsmI GAATG_Cn' Cuts at: 2092 2215 3590 3683 2092 Size: 123 1375 93 4488 Fragments arranged by size: 4488 1375 123 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2131 2131 Size: 6079 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2073 2079 2394 2893 3021 3038 3140 Size: 399 283 6 315 499 128 17 102 Cuts at: 3140 3279 3292 3343 3398 4140 4225 5296 1391 Size: 139 13 51 55 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 499 399 315 283 139 128 102 85 55 51 17 13 6 BspEI T'CCGG_A Cuts at: 5742 5742 Size: 6079 BspHI T'CATG_A Cuts at: 3968 5698 3968 Size: 1730 4349 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3434 844 Size: 2590 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3966 1394 Size: 429 2143 3507 Fragments arranged by size: 3507 2143 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4574 4748 65 Size: 1183 3326 174 1396 Fragments arranged by size: 3326 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2193 2295 2325 2880 4723 4910 1700 Size: 110 383 102 30 555 1843 187 2869 Fragments arranged by size: 2869 1843 555 383 187 110 102 30 BsrGI T'GTAC_A Cuts at: 96 2275 2359 96 Size: 2179 84 3816 Fragments arranged by size: 3816 2179 84 BssHII G'CGCG_C Cuts at: 1925 2144 1925 Size: 219 5860 BssSI C'ACGA_G Cuts at: 4139 5151 4139 Size: 1012 5067 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6079 BstBI TT'CG_AA Cuts at: 4883 4883 Size: 6079 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6079 BstXI CCAn_nnnn'nTGG Cuts at: 2900 3268 3316 2900 Size: 368 48 5663 Fragments arranged by size: 5663 368 48 BstYI r'GATC_y Cuts at: 1972 2547 2721 3117 3409 3776 4161 4178 5630 Size: 575 174 396 292 367 385 17 1452 Cuts at: 5630 5716 5728 6074 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 575 396 385 367 346 292 174 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2980 513 Size: 552 939 976 3612 Fragments arranged by size: 3612 976 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2986 487 Size: 683 56 1760 3580 Fragments arranged by size: 3580 1760 683 56 BtsI GCAGTG_nn' Cuts at: 3595 4389 4409 3595 Size: 794 20 5265 Fragments arranged by size: 5265 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6079 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6044 DraI TTT'AAA Cuts at: 3383 3383 Size: 6079 DraIII CAC_nnn'GTG Cuts at: 2284 2284 Size: 6079 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5086 809 Size: 507 3770 1802 Fragments arranged by size: 3770 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2091 2802 3526 Size: 54 1425 50 275 18 261 711 724 Cuts at: 3526 4415 6044 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 724 711 275 261 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2091 3526 1537 Size: 275 279 1435 4090 Fragments arranged by size: 4090 1435 279 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2660 4009 1353 Size: 1307 1349 3423 Fragments arranged by size: 3423 1349 1307 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2121 4666 5040 5186 387 Size: 1064 138 532 2545 374 146 1280 Fragments arranged by size: 2545 1280 1064 532 374 146 138 EcoICRI GAG'CTC Cuts at: 2392 3019 2392 Size: 627 5452 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 2244 3050 3134 4142 4739 5526 Size: 393 399 213 806 84 1008 597 787 Cuts at: 5526 5794 1239 Size: 268 1524 Fragments arranged by size: 1524 1008 806 787 597 399 393 268 213 84 EcoNI CCTnn'n_nnAGG Cuts at: 2870 2870 Size: 6079 EcoO109I rG'GnC_Cy Cuts at: 2889 3154 2889 Size: 265 5814 EcoRI G'AATT_C Cuts at: 2267 3388 3766 2267 Size: 1121 378 4580 Fragments arranged by size: 4580 1121 378 FspI TGC'GCA Cuts at: 4585 4585 Size: 6079 HaeII r_GCGC'y Cuts at: 1678 2336 5226 1678 Size: 658 2890 2531 Fragments arranged by size: 2890 2531 658 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2221 2253 2767 2799 2909 Size: 32 135 32 649 32 514 32 110 Cuts at: 2909 2941 4720 4752 4794 4826 1373 Size: 32 1779 32 42 32 2626 Fragments arranged by size: 2626 1779 649 514 135 110 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3423 3669 669 Size: 988 281 1485 246 3079 Fragments arranged by size: 3079 1485 988 281 246 HindIII A'AGCT_T Cuts at: 748 2807 748 Size: 2059 4020 HpaI GTT'AAC Cuts at: 3669 3669 Size: 6079 KpnI G_GTAC'C Cuts at: 3404 3404 Size: 6079 MfeI C'AATT_G Cuts at: 3678 3678 Size: 6079 MluI A'CGCG_T Cuts at: 5955 5955 Size: 6079 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5193 5377 1305 Size: 3888 184 2007 Fragments arranged by size: 3888 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2804 10 Size: 54 1425 343 972 3285 Fragments arranged by size: 3285 1425 972 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2528 2744 3314 4037 4396 4555 518 Size: 648 1362 216 570 723 359 159 2042 Fragments arranged by size: 2042 1362 723 648 570 359 216 159 MspA1I CmG'CkG Cuts at: 1606 2096 2982 3061 3495 4172 5320 5565 1606 Size: 490 886 79 434 677 1148 245 2120 Fragments arranged by size: 2120 1148 886 677 490 434 245 79 NaeI GCC'GGC Cuts at: 2195 2195 Size: 6079 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4588 Fragments arranged by size: 4588 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6079 NgoMIV G'CCGG_C Cuts at: 2193 2193 Size: 6079 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6079 NotI GC'GGCC_GC Cuts at: 2091 3526 2091 Size: 1435 4644 NspI r_CATG'y Cuts at: 1132 1229 3437 4982 1132 Size: 97 2208 1545 2229 Fragments arranged by size: 2229 2208 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6079 PciI A'CATG_T Cuts at: 4978 4978 Size: 6079 PflMI CCAn_nnn'nTGG Cuts at: 1108 2625 1108 Size: 1517 4562 PfoI T'CCnGG_A Cuts at: 2814 2814 Size: 6079 PmeI GTTT'AAAC Cuts at: 3383 3383 Size: 6079 PmlI CAC'GTG Cuts at: 3244 3244 Size: 6079 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4148 4180 5661 5693 4148 Size: 32 1481 32 4534 Fragments arranged by size: 4534 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3154 3154 Size: 6079 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6079 PsiI TTA'TAA Cuts at: 3649 3649 Size: 6079 PspOMI G'GGCC_C Cuts at: 2075 2889 2075 Size: 814 5265 PstI C_TGCA'G Cuts at: 830 3431 830 Size: 2601 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6079 SacI G_AGCT'C Cuts at: 2394 3021 2394 Size: 627 5452 SacII CC_GC'GG Cuts at: 2983 2983 Size: 6079 SalI G'TCGA_C Cuts at: 1936 3421 1936 Size: 1485 4594 SapI GCTCTTCn'nnn_ Cuts at: 2660 2660 Size: 6079 SbfI CC_TGCA'GG Cuts at: 3431 3431 Size: 6079 ScaI AGT'ACT Cuts at: 2639 3227 4327 6039 2639 Size: 588 1100 1712 2679 Fragments arranged by size: 2679 1712 1100 588 SfcI C'TryA_G Cuts at: 826 1045 3427 4562 5243 5434 826 Size: 219 2382 1135 681 191 1471 Fragments arranged by size: 2382 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6079 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6079 SmaI CCC'GGG Cuts at: 1872 2074 2080 2118 2713 3406 1872 Size: 202 6 38 595 693 4545 Fragments arranged by size: 4545 693 595 202 38 6 SmlI C'TyrA_G Cuts at: 820 1017 1943 2238 2645 3332 4183 5084 5346 Size: 197 926 295 407 687 851 901 262 Cuts at: 5346 820 Size: 1553 Fragments arranged by size: 1553 926 901 851 687 407 295 262 197 SnaBI TAC'GTA Cuts at: 493 3870 493 Size: 3377 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6079 SphI G_CATG'C Cuts at: 1132 3437 1132 Size: 2305 3774 SrfI GCCC'GGGC Cuts at: 2074 2074 Size: 6079 SspI AAT'ATT Cuts at: 5 52 4003 5 Size: 47 3951 2081 Fragments arranged by size: 3951 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6079 StyI C'CwwG_G Cuts at: 513 1065 2004 2499 2526 2913 3518 513 Size: 552 939 495 27 387 605 3074 Fragments arranged by size: 3074 939 605 552 495 387 27 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3094 3194 3274 3322 4106 4123 4276 Size: 1255 1514 100 80 48 784 17 153 Cuts at: 4276 4461 4906 325 Size: 185 445 1498 Fragments arranged by size: 1514 1498 1255 784 445 185 153 100 80 48 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2275 2359 2637 Size: 274 80 33 51 1443 298 84 278 Cuts at: 2637 2649 2832 3225 4325 6037 96 Size: 12 183 393 1100 1712 138 Fragments arranged by size: 1712 1443 1100 393 298 278 274 183 138 84 80 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2928 2960 6039 6071 2928 Size: 32 3079 32 2936 Fragments arranged by size: 3079 2936 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3323 1316 Size: 2007 4072 XbaI T'CTAG_A Cuts at: 3415 3415 Size: 6079 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2497 2812 2497 Size: 315 5764 XhoI C'TCGA_G Cuts at: 1943 2238 1943 Size: 295 5784 XmaI C'CCGG_G Cuts at: 1870 2072 2078 2116 2711 3404 1870 Size: 202 6 38 595 693 4545 Fragments arranged by size: 4545 693 595 202 38 6 XmnI GAAnn'nnTTC Cuts at: 4208 4208 Size: 6079 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3887 276 Size: 53 83 186 936 2353 2468 Fragments arranged by size: 2468 2353 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SfoI SwaI