(Circular) (Six-base) MAPSORT of: pFN21AB7758.seq Check: 9932 from: 1 to: 6199 pFN21AB7758 6199 bp With 182 enzymes: SgfI * October 20, 2009 10:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2996 2996 Size: 6199 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2769 4009 278 Size: 53 83 186 936 1233 1240 2468 Fragments arranged by size: 2468 1240 1233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2025 3542 1937 Size: 88 1517 4594 Fragments arranged by size: 4594 1517 88 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4262 5646 5914 1632 Size: 2630 1384 268 1917 Fragments arranged by size: 2630 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 3248 5098 6075 1203 Size: 2045 1850 977 1327 Fragments arranged by size: 2045 1850 1327 977 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6199 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6199 AlwNI CAG_nnn'CTG Cuts at: 2106 2455 3942 5514 2106 Size: 349 1487 1572 2791 Fragments arranged by size: 2791 1572 1487 349 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 3001 3508 3734 3886 5995 1493 Size: 1508 507 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 507 226 152 AseI AT'TA_AT Cuts at: 160 1032 4753 160 Size: 872 3721 1606 Fragments arranged by size: 3721 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 3524 1870 Size: 73 1581 4545 Fragments arranged by size: 4545 1581 73 BamHI G'GATC_C Cuts at: 3105 3529 3896 3105 Size: 424 367 5408 Fragments arranged by size: 5408 424 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3520 4975 618 Size: 325 217 627 1733 1455 1842 Fragments arranged by size: 1842 1733 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2067 2267 3155 3497 3518 1391 Size: 676 200 888 342 21 4072 Fragments arranged by size: 4072 888 676 342 200 21 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3028 928 Size: 971 1129 4099 Fragments arranged by size: 4099 1129 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4390 4424 1903 Size: 34 2453 34 3678 Fragments arranged by size: 3678 2453 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4091 5301 4091 Size: 1210 4989 BclI T'GATC_A Cuts at: 1548 2145 1548 Size: 597 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2319 2710 2848 6194 2319 Size: 391 138 3346 2324 Fragments arranged by size: 3346 2324 391 138 BlpI GC'TnA_GC Cuts at: 3616 3616 Size: 6199 Bme1580I G_kGCm'C Cuts at: 1790 3488 4260 5416 1790 Size: 1698 772 1156 2573 Fragments arranged by size: 2573 1698 1156 772 BmgBI CAC'GTC Cuts at: 1318 2911 1318 Size: 1593 4606 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2911 3082 3235 Size: 416 53 83 399 24 1494 171 153 Cuts at: 3235 3930 4888 442 Size: 695 958 1753 Fragments arranged by size: 1753 1494 958 695 416 399 171 153 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3292 3292 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2081 2300 2639 2699 3184 4859 1239 Size: 842 219 339 60 485 1675 2579 Fragments arranged by size: 2579 1675 842 485 339 219 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2137 4324 5189 5487 2137 Size: 2187 865 298 2849 Fragments arranged by size: 2849 2187 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4862 882 Size: 617 3363 2219 Fragments arranged by size: 3363 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3990 493 Size: 711 2786 2702 Fragments arranged by size: 2786 2702 711 BsaBI GATnn'nnATC Cuts at: 6193 6193 Size: 6199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2766 4006 4388 5023 Size: 53 83 186 936 1233 1240 382 635 Cuts at: 5023 275 Size: 1451 Fragments arranged by size: 1451 1240 1233 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4632 5030 5304 5451 5862 4632 Size: 398 274 147 411 4969 Fragments arranged by size: 4969 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2609 3265 3268 1186 Size: 1423 656 3 4117 Fragments arranged by size: 4117 1423 656 3 BseYI C'CCAG_C Cuts at: 2352 5402 5898 2352 Size: 3050 496 2653 Fragments arranged by size: 3050 2653 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2974 1163 Size: 573 1238 4388 Fragments arranged by size: 4388 1238 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3649 4410 5438 1540 Size: 275 186 1648 761 1028 2301 Fragments arranged by size: 2301 1648 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2067 2301 3155 3397 3497 4260 4345 5416 2067 Size: 234 854 242 100 763 85 1071 2850 Fragments arranged by size: 2850 1071 854 763 242 234 100 85 BsmI GAATG_Cn' Cuts at: 3710 3803 3710 Size: 93 6106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2067 2267 2301 3155 3397 3488 3497 Size: 399 277 200 34 854 242 91 9 Cuts at: 3497 3518 4260 4345 5416 1391 Size: 21 742 85 1071 2174 Fragments arranged by size: 2174 1071 854 742 399 277 242 200 91 85 34 21 9 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 2769 4088 5818 2769 Size: 1319 1730 3150 Fragments arranged by size: 3150 1730 1319 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2054 2428 2996 3442 3554 844 Size: 1210 374 568 446 112 3489 Fragments arranged by size: 3489 1210 568 446 374 112 BsrBI CCG'CTC Cuts at: 1394 1823 4086 1394 Size: 429 2263 3507 Fragments arranged by size: 3507 2263 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4694 4868 65 Size: 1183 3446 174 1396 Fragments arranged by size: 3446 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4843 5030 1700 Size: 110 3033 187 2869 Fragments arranged by size: 3033 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 4259 5271 4259 Size: 1012 5187 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6199 BstBI TT'CG_AA Cuts at: 2939 5003 2939 Size: 2064 4135 BstEII G'GTnAC_C Cuts at: 1184 2041 2384 1184 Size: 857 343 4999 Fragments arranged by size: 4999 857 343 BstXI CCAn_nnnn'nTGG Cuts at: 2059 2059 Size: 6199 BstYI r'GATC_y Cuts at: 1972 2319 2710 2848 3105 3529 3896 4281 4298 Size: 347 391 138 257 424 367 385 17 Cuts at: 4298 5750 5836 5848 6194 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 424 391 385 367 347 346 257 138 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2302 3388 513 Size: 552 939 298 1086 3324 Fragments arranged by size: 3324 1086 939 552 298 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5460 Fragments arranged by size: 5460 683 56 BtsI GCAGTG_nn' Cuts at: 2121 2935 3715 4509 4529 2121 Size: 814 780 794 20 3791 Fragments arranged by size: 3791 814 794 780 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 3503 3503 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2672 5206 809 Size: 507 1356 2534 1802 Fragments arranged by size: 2534 1802 1356 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2226 3646 Size: 54 1425 50 275 18 265 131 1420 Cuts at: 3646 4535 6164 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1420 889 275 265 131 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 3061 3143 3286 4129 1353 Size: 923 785 82 143 843 3423 Fragments arranged by size: 3423 923 843 785 143 82 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4786 5160 5306 387 Size: 1064 138 3197 374 146 1280 Fragments arranged by size: 3197 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2065 3153 3495 2065 Size: 1088 342 4769 Fragments arranged by size: 4769 1088 342 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2081 2300 2639 2699 3184 4262 4859 Size: 393 449 219 339 60 485 1078 597 Cuts at: 4859 5646 5914 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1078 787 597 485 449 393 339 268 219 60 EcoRI G'AATT_C Cuts at: 3001 3508 3886 3001 Size: 507 378 5314 Fragments arranged by size: 5314 507 378 FspI TGC'GCA Cuts at: 4705 4705 Size: 6199 HaeII r_GCGC'y Cuts at: 1678 5346 1678 Size: 3668 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2062 2094 2905 2937 3263 Size: 32 135 32 490 32 811 32 326 Cuts at: 3263 3295 3384 3416 4840 4872 4914 4946 1373 Size: 32 89 32 1424 32 42 32 2626 Fragments arranged by size: 2626 1424 811 490 326 135 89 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3543 3789 669 Size: 988 281 1605 246 3079 Fragments arranged by size: 3079 1605 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6199 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2233 5313 5497 1305 Size: 928 3080 184 2007 Fragments arranged by size: 3080 2007 928 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2097 2228 10 Size: 54 1425 343 265 131 3981 Fragments arranged by size: 3981 1425 343 265 131 54 MslI CAynn'nnrTG Cuts at: 518 1166 3479 4157 4516 4675 518 Size: 648 2313 678 359 159 2042 Fragments arranged by size: 2313 2042 678 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2169 2671 2719 3615 4292 5440 5685 1606 Size: 563 502 48 896 677 1148 245 2120 Fragments arranged by size: 2120 1148 896 677 563 502 245 48 NcoI C'CATG_G Cuts at: 513 1065 2004 2302 513 Size: 552 939 298 4410 Fragments arranged by size: 4410 939 552 298 NdeI CA'TA_TG Cuts at: 387 387 Size: 6199 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 3252 3557 5102 1132 Size: 97 2023 305 1545 2229 Fragments arranged by size: 2229 2023 1545 305 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6199 PciI A'CATG_T Cuts at: 3248 5098 3248 Size: 1850 4349 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 5781 5813 4268 Size: 32 1481 32 4654 Fragments arranged by size: 4654 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2888 1613 Size: 1275 4924 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PstI C_TGCA'G Cuts at: 830 2439 2451 2754 3551 830 Size: 1609 12 303 797 3478 Fragments arranged by size: 3478 1609 797 303 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 PvuII CAG'CTG Cuts at: 2169 2671 2169 Size: 502 5697 SacI G_AGCT'C Cuts at: 2067 3155 3497 2067 Size: 1088 342 4769 Fragments arranged by size: 4769 1088 342 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SapI GCTCTTCn'nnn_ Cuts at: 3143 3143 Size: 6199 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 2897 4447 6159 2897 Size: 1550 1712 2937 Fragments arranged by size: 2937 1712 1550 SfcI C'TryA_G Cuts at: 826 1045 2435 2447 2661 2750 3547 4682 5363 Size: 219 1390 12 214 89 797 1135 681 Cuts at: 5363 5554 826 Size: 191 1471 Fragments arranged by size: 1471 1390 1135 797 681 219 214 191 89 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 3526 1872 Size: 1654 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2152 4303 5204 5466 820 Size: 197 926 209 2151 901 262 1553 Fragments arranged by size: 2151 1553 926 901 262 209 197 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 3050 3068 4123 5 Size: 47 2998 18 1055 2081 Fragments arranged by size: 2998 2081 1055 47 18 StuI AGG'CCT Cuts at: 1517 1517 Size: 6199 StyI C'CwwG_G Cuts at: 513 1065 2004 2302 2380 3291 3455 3638 513 Size: 552 939 298 78 911 164 183 3074 Fragments arranged by size: 3074 939 911 552 298 183 164 78 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2893 4226 4243 4396 4581 5026 325 Size: 1255 1313 1333 17 153 185 445 1498 Fragments arranged by size: 1498 1333 1313 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2895 4445 6157 Size: 274 80 33 51 1443 918 1550 1712 Cuts at: 6157 96 Size: 138 Fragments arranged by size: 1712 1550 1443 918 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6159 6191 6159 Size: 32 6167 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6199 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 3524 1870 Size: 1654 4545 XmnI GAAnn'nnTTC Cuts at: 2561 4328 2561 Size: 1767 4432 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2767 4007 276 Size: 53 83 186 936 1233 1240 2468 Fragments arranged by size: 2468 1240 1233 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI