Restriction map for
pFN21AB7756
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BbeI BbrPI BbvCI BcgI
BclI BfrI BglI BglII BlnI
BsaAI BsaBI BsaHI BseAI BsiEI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT14I FauI FbaI FseI HaeII
HgaI Hin1I HincII HindII HpaI
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MspA1I MunI MwoI NaeI NarI
NcoI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AccI 168 GTCTAC GT^[AC][GT]AC
AccII 1092 CGCG CG^CG
ApaI 97 GGGCCC GGGCC^C
AvaI 101 CCCGGG C^[CT]CG[GA]G
AvaII 428 GGACC G^G[AT]CC
AviII 1033 TGCGCA TGC^GCA
BamHI 106 GGATCC G^GATCC
BanI 158 GGTGCC G^G[CT][GA]CC
BbsI 304 GAAGAC GAAGAC(2/6)
BciVI 197 GGATAC GTATCC(6/5)
BfaI 229 CTAG C^TAG
BpuAI 304 GAAGAC GAAGAC(2/6)
BseRI 85 CTCCTC GAGGAG(10/8)
BsoBI 101 CCCGGG C^[CT]CG[GA]G
BspMI 308 ACCTGC ACCTGC(4/8)
BssSI 163 CTCGTG CACGAG(-5/-1)
BstUI 1092 CGCG CG^CG
BstYI 106 GGATCC [GA]^GATC[CT]
Cac8I 360 GCAAGC GC[ACGT]^[ACGT]GC
EcoNI 22 CCTTCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 890 GAATTC G^AATTC
EcoT22I 578 ATGCAT ATGCA^T
FspI 1033 TGCGCA TGC^GCA
HindIII 374 AAGCTT A^AGCTT
MaeI 229 CTAG C^TAG
MflI 106 GGATCC [GA]^GATC[CT]
MseI 1361 TTAA T^TAA
MvnI 1092 CGCG CG^CG
NdeI 575 CATATG CA^TATG
NsiI 578 ATGCAT ATGCA^T
NspI 580 GCATGT [GA]CATG^[CT]
ScaI 15 AGTACT AGT^ACT
SmaI 101 CCCGGG CCC^GGG
SmlI 439 CTTGAG C^T[CT][GA]AG
Tru9I 1361 TTAA T^TAA
XhoII 106 GGATCC [GA]^GATC[CT]
XmaCI 101 CCCGGG C^CCGGG
XmaI 101 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AciI 475 CCGC CCGC(-3/-1)
1091 CCGC
AcsI 890 GAATTC [GA]^AATT[CT]
1270 AAATTC
AfaI 16 GTAC GT^AC
1202 GTAC
ApoI 890 GAATTC [GA]^AATT[CT]
1270 AAATTC
BlpI 866 GCTCAGC GC^T[ACGT]AGC
1286 GCTCAGC
Bpu1102I 866 GCTCAGC GC^T[ACGT]AGC
1286 GCTCAGC
BstF5I 1373 CATCC GGATG(2/0)
290 GGATG
CelII 866 GCTCAGC GC^T[ACGT]AGC
1286 GCTCAGC
CfoI 284 GCGC GCG^C
1034 GCGC
DpnII 107 GATC ^GATC
347 GATC
DraII 30 AGGCCCT [GA]G^G[ACGT]CC[CT]
97 GGGCCCC
EcoO109I 30 AGGCCCT [GA]G^G[ACGT]CC[CT]
97 GGGCCCC
FokI 1373 CATCC GGATG(9/13)
290 GGATG
HhaI 284 GCGC GCG^C
1034 GCGC
HinP1I 284 GCGC G^CGC
1034 GCGC
HphI 349 TCACC GGTGA(8/7)
791 TCACC
MboI 107 GATC ^GATC
347 GATC
NdeII 107 GATC ^GATC
347 GATC
RsaI 16 GTAC GT^AC
1202 GTAC
Sau3AI 107 GATC ^GATC
347 GATC
SfcI 828 CTACAG C^T[GA][CT]AG
1080 CTACAG
Tsp509I 891 AATT ^AATT
1271 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AluI AlwI AlwNI Asp700I AspHI
BanII BbvI BcnI BmyI BpmI
BsaI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
Bsp1286I BsrI BssKI BstNI Cfr13I
DdeI DsaI EarI EcoRII Fnu4HI
HaeIII HapII HinfI HpaII ItaI
Ksp632I MaeIII MboII MnlI MslI
MspI MvaI NciI NlaIII NlaIV
PleI SacI SapI Sau96I ScrFI
SfaNI StuI TfiI TseI Tsp45I
TspRI XmnI