(Circular) (Six-base) MAPSORT of: pFN21AB7756.seq Check: 5699 from: 1 to: 6199 pFN21AB7756 6199 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4009 278 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2174 3542 1937 Size: 237 1368 4594 Fragments arranged by size: 4594 1368 237 Acc65I G'GTAC_C Cuts at: 3520 3520 Size: 6199 AclI AA'CG_TT Cuts at: 1563 4326 4699 1563 Size: 2763 373 3063 Fragments arranged by size: 3063 2763 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2107 2397 2593 2677 2761 2845 3013 3097 Size: 475 290 196 84 84 84 168 84 Cuts at: 3097 3181 3265 4262 5646 5914 1632 Size: 84 84 997 1384 268 1917 Fragments arranged by size: 1917 1384 997 475 290 268 196 168 84 84 84 84 84 84 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6002 AflIII A'CryG_T Cuts at: 1203 5098 6075 1203 Size: 3895 977 1327 Fragments arranged by size: 3895 1327 977 AgeI A'CCGG_T Cuts at: 5030 5030 Size: 6199 AhdI GACnn_n'nnGTC Cuts at: 4928 4928 Size: 6199 AleI CACnn'nnGTG Cuts at: 1166 3410 1166 Size: 2244 3955 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6199 AlwNI CAG_nnn'CTG Cuts at: 2039 2616 2868 2931 3036 3942 5514 2039 Size: 577 252 63 105 906 1572 2724 Fragments arranged by size: 2724 1572 906 577 252 105 63 ApaI G_GGCC'C Cuts at: 2106 2106 Size: 6199 ApaLI G'TGCA_C Cuts at: 4256 5412 4256 Size: 1156 5043 ApoI r'AATT_y Cuts at: 1493 2895 3275 3508 3734 3886 5995 1493 Size: 1402 380 233 226 152 2109 1697 Fragments arranged by size: 2109 1697 1402 380 233 226 152 AseI AT'TA_AT Cuts at: 160 1032 4753 160 Size: 872 3721 1606 Fragments arranged by size: 3721 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 AvaI C'yCGr_G Cuts at: 1870 1943 2106 3524 1870 Size: 73 163 1418 4545 Fragments arranged by size: 4545 1418 163 73 BamHI G'GATC_C Cuts at: 2111 3529 3896 2111 Size: 1418 367 4414 Fragments arranged by size: 4414 1418 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2163 3520 4975 618 Size: 325 217 627 376 1357 1455 1842 Fragments arranged by size: 1842 1455 1357 627 376 325 217 BanII G_rGCy'C Cuts at: 1391 2106 2705 3293 3377 3518 1391 Size: 715 599 588 84 141 4072 Fragments arranged by size: 4072 715 599 588 141 84 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2316 928 Size: 971 417 4811 Fragments arranged by size: 4811 971 417 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4390 4424 1903 Size: 34 50 34 2369 34 3678 Fragments arranged by size: 3678 2369 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2196 4091 5301 2196 Size: 1895 1210 3094 Fragments arranged by size: 3094 1895 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6199 BfrBI ATG'CAT Cuts at: 2585 2585 Size: 6199 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4810 136 Size: 107 122 71 631 605 3138 1525 Fragments arranged by size: 3138 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6194 6194 Size: 6199 BlpI GC'TnA_GC Cuts at: 2872 3292 3616 2872 Size: 420 324 5455 Fragments arranged by size: 5455 420 324 Bme1580I G_kGCm'C Cuts at: 1790 2106 2496 4260 5416 1790 Size: 316 390 1764 1156 2573 Fragments arranged by size: 2573 1764 1156 390 316 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6199 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2263 3165 3930 Size: 416 53 83 399 24 846 902 765 Cuts at: 3930 4888 442 Size: 958 1753 Fragments arranged by size: 1753 958 902 846 765 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6199 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2724 2724 Size: 6199 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2030 2313 2321 2673 2757 4859 1239 Size: 791 283 8 352 84 2102 2579 Fragments arranged by size: 2579 2102 791 352 283 84 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2465 4324 5189 5487 2465 Size: 1859 865 298 3177 Fragments arranged by size: 3177 1859 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2093 2458 2500 4862 882 Size: 617 594 365 42 2362 2219 Fragments arranged by size: 2362 2219 617 594 365 42 BsaAI yAC'GTr Cuts at: 493 1204 3990 493 Size: 711 2786 2702 Fragments arranged by size: 2786 2702 711 BsaBI GATnn'nnATC Cuts at: 6193 6193 Size: 6199 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4006 4388 5023 275 Size: 53 83 186 936 2473 382 635 1451 Fragments arranged by size: 2473 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2819 2903 3323 3450 4632 5030 5304 5451 5862 Size: 84 420 127 1182 398 274 147 411 Cuts at: 5862 2819 Size: 3156 Fragments arranged by size: 3156 1182 420 411 398 274 147 127 84 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5974 Fragments arranged by size: 5974 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2081 1186 Size: 895 5304 BseYI C'CCAG_C Cuts at: 2099 2250 5402 5898 2099 Size: 151 3152 496 2400 Fragments arranged by size: 3152 2400 496 151 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2692 2776 3028 3196 3475 1163 Size: 573 956 84 252 168 279 3887 Fragments arranged by size: 3887 956 573 279 252 168 84 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3649 4410 5438 1540 Size: 275 186 1648 761 1028 2301 Fragments arranged by size: 2301 1648 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2705 3293 3377 4260 4345 5416 2705 Size: 588 84 883 85 1071 3488 Fragments arranged by size: 3488 1071 883 588 85 84 BsmI GAATG_Cn' Cuts at: 2791 2927 3431 3710 3803 2791 Size: 136 504 279 93 5187 Fragments arranged by size: 5187 504 279 136 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2106 2496 2705 3293 3377 3518 4260 Size: 399 316 390 209 588 84 141 742 Cuts at: 4260 4345 5416 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 588 399 390 316 209 141 85 84 BspEI T'CCGG_A Cuts at: 5862 5862 Size: 6199 BspHI T'CATG_A Cuts at: 4088 5818 4088 Size: 1730 4469 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2322 3554 844 Size: 1478 1232 3489 Fragments arranged by size: 3489 1478 1232 BsrBI CCG'CTC Cuts at: 1394 1823 4086 1394 Size: 429 2263 3507 Fragments arranged by size: 3507 2263 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4694 4868 65 Size: 1183 3446 174 1396 Fragments arranged by size: 3446 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4843 5030 1700 Size: 110 3033 187 2869 Fragments arranged by size: 3033 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6199 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6199 BssSI C'ACGA_G Cuts at: 2168 4259 5271 2168 Size: 2091 1012 3096 Fragments arranged by size: 3096 2091 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6199 BstBI TT'CG_AA Cuts at: 5003 5003 Size: 6199 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6199 BstYI r'GATC_y Cuts at: 1972 2111 3529 3896 4281 4298 5750 5836 5848 Size: 139 1418 367 385 17 1452 86 12 Cuts at: 5848 6194 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1418 385 367 346 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2180 2428 2496 513 Size: 552 1115 248 68 4216 Fragments arranged by size: 4216 1115 552 248 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5460 Fragments arranged by size: 5460 683 56 BtsI GCAGTG_nn' Cuts at: 2134 2680 2764 3715 4509 4529 2134 Size: 546 84 951 794 20 3804 Fragments arranged by size: 3804 951 794 546 84 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6199 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6164 DraI TTT'AAA Cuts at: 3503 3503 Size: 6199 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5206 809 Size: 507 3890 1802 Fragments arranged by size: 3890 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3646 4535 6164 Size: 54 1425 50 275 18 1816 889 1629 Cuts at: 6164 8 Size: 43 Fragments arranged by size: 1816 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3646 1537 Size: 275 1834 4090 Fragments arranged by size: 4090 1834 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2444 2636 2764 4129 1353 Size: 1091 192 128 1365 3423 Fragments arranged by size: 3423 1365 1091 192 128 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4786 5160 5306 387 Size: 1064 138 3197 374 146 1280 Fragments arranged by size: 3197 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2703 3291 3375 2703 Size: 588 84 5527 Fragments arranged by size: 5527 588 84 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2030 2107 2313 2321 2397 2593 2673 Size: 393 398 77 206 8 76 196 80 Cuts at: 2673 2677 2757 2761 2845 3013 3097 3181 3265 Size: 4 80 4 84 168 84 84 84 Cuts at: 3265 4262 4859 5646 5914 1239 Size: 997 597 787 268 1524 Fragments arranged by size: 1524 997 787 597 398 393 268 206 196 168 84 84 84 84 80 80 77 76 8 4 4 EcoNI CCTnn'n_nnAGG Cuts at: 2031 2031 Size: 6199 EcoO109I rG'GnC_Cy Cuts at: 2036 2103 2036 Size: 67 6132 EcoRI G'AATT_C Cuts at: 2895 3508 3886 2895 Size: 613 378 5208 Fragments arranged by size: 5208 613 378 FspI TGC'GCA Cuts at: 3040 4705 3040 Size: 1665 4534 HaeII r_GCGC'y Cuts at: 1678 5346 1678 Size: 3668 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2443 2457 2475 2489 3196 Size: 32 135 32 871 14 18 14 707 Cuts at: 3196 3228 4840 4872 4914 4946 1373 Size: 32 1612 32 42 32 2626 Fragments arranged by size: 2626 1612 871 707 135 42 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 3543 3789 669 Size: 988 281 1605 246 3079 Fragments arranged by size: 3079 1605 988 281 246 HindIII A'AGCT_T Cuts at: 748 2379 748 Size: 1631 4568 HpaI GTT'AAC Cuts at: 3789 3789 Size: 6199 KpnI G_GTAC'C Cuts at: 3524 3524 Size: 6199 MfeI C'AATT_G Cuts at: 3798 3798 Size: 6199 MluI A'CGCG_T Cuts at: 6075 6075 Size: 6199 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2253 5313 5497 1305 Size: 948 3060 184 2007 Fragments arranged by size: 3060 2007 948 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4377 Fragments arranged by size: 4377 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2584 3256 3410 4157 4516 4675 518 Size: 648 1418 672 154 747 359 159 2042 Fragments arranged by size: 2042 1418 747 672 648 359 159 154 MspA1I CmG'CkG Cuts at: 1606 3615 4292 5440 5685 1606 Size: 2009 677 1148 245 2120 Fragments arranged by size: 2120 2009 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5647 NdeI CA'TA_TG Cuts at: 387 2581 387 Size: 2194 4005 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6199 NotI GC'GGCC_GC Cuts at: 3646 3646 Size: 6199 NsiI A_TGCA'T Cuts at: 2587 2587 Size: 6199 NspI r_CATG'y Cuts at: 1132 1229 2589 3557 5102 1132 Size: 97 1360 968 1545 2229 Fragments arranged by size: 2229 1545 1360 968 97 PasI CC'CwG_GG Cuts at: 1348 2414 1348 Size: 1066 5133 PciI A'CATG_T Cuts at: 5098 5098 Size: 6199 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6199 PfoI T'CCnGG_A Cuts at: 2216 2216 Size: 6199 PmeI GTTT'AAAC Cuts at: 3503 3503 Size: 6199 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4268 4300 5781 5813 4268 Size: 32 1481 32 4654 Fragments arranged by size: 4654 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6199 PsiI TTA'TAA Cuts at: 3769 3769 Size: 6199 PspOMI G'GGCC_C Cuts at: 2102 2102 Size: 6199 PstI C_TGCA'G Cuts at: 830 3551 830 Size: 2721 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6199 SacI G_AGCT'C Cuts at: 2705 3293 3377 2705 Size: 588 84 5527 Fragments arranged by size: 5527 588 84 SalI G'TCGA_C Cuts at: 1936 3541 1936 Size: 1605 4594 SapI GCTCTTCn'nnn_ Cuts at: 2444 2636 2764 2444 Size: 192 128 5879 Fragments arranged by size: 5879 192 128 SbfI CC_TGCA'GG Cuts at: 3551 3551 Size: 6199 ScaI AGT'ACT Cuts at: 2022 4447 6159 2022 Size: 2425 1712 2062 Fragments arranged by size: 2425 2062 1712 SfcI C'TryA_G Cuts at: 826 1045 2833 3085 3547 4682 5363 5554 826 Size: 219 1788 252 462 1135 681 191 1471 Fragments arranged by size: 1788 1471 1135 681 462 252 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6199 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6199 SmaI CCC'GGG Cuts at: 1872 2108 3526 1872 Size: 236 1418 4545 Fragments arranged by size: 4545 1418 236 SmlI C'TyrA_G Cuts at: 820 1017 1943 2444 4303 5204 5466 820 Size: 197 926 501 1859 901 262 1553 Fragments arranged by size: 1859 1553 926 901 501 262 197 SnaBI TAC'GTA Cuts at: 493 3990 493 Size: 3497 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6199 SphI G_CATG'C Cuts at: 1132 3557 1132 Size: 2425 3774 SspI AAT'ATT Cuts at: 5 52 4123 5 Size: 47 4071 2081 Fragments arranged by size: 4071 2081 47 StuI AGG'CCT Cuts at: 1517 2690 2942 3026 3110 3194 3446 1517 Size: 1173 252 84 84 84 252 4270 Fragments arranged by size: 4270 1173 252 252 84 84 84 StyI C'CwwG_G Cuts at: 513 1065 3638 513 Size: 552 2573 3074 Fragments arranged by size: 3074 2573 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4226 4243 4396 4581 5026 325 Size: 1255 2646 17 153 185 445 1498 Fragments arranged by size: 2646 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2020 4445 6157 Size: 274 80 33 51 1443 43 2425 1712 Cuts at: 6157 96 Size: 138 Fragments arranged by size: 2425 1712 1443 274 138 80 51 43 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6159 6191 6159 Size: 32 6167 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6199 XbaI T'CTAG_A Cuts at: 3535 3535 Size: 6199 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6199 XmaI C'CCGG_G Cuts at: 1870 2106 3524 1870 Size: 236 1418 4545 Fragments arranged by size: 4545 1418 236 XmnI GAAnn'nnTTC Cuts at: 2453 2690 2858 2942 3026 3110 3194 3278 3446 Size: 237 168 84 84 84 84 84 168 Cuts at: 3446 4328 2453 Size: 882 4324 Fragments arranged by size: 4324 882 237 168 168 84 84 84 84 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4007 276 Size: 53 83 186 936 2473 2468 Fragments arranged by size: 2473 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI