Restriction map for
pFN21AB7756
(Linear) (Six-base) MAPSORT of: KIBB7756.seq Check: 9227 from: 1 to: 1494
KIBB7756.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AccI GT'mk_AC
Cuts at: 0 169 1494
Size: 169 1325
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 102 392 588 672 756 840 1008 1092
Size: 102 290 196 84 84 84 168 84
Cuts at: 1092 1176 1260 1494
Size: 84 84 234
Fragments arranged by size:
290 234 196 168 102 84 84 84
84 84 84
AleI CACnn'nnGTG
Cuts at: 0 1405 1494
Size: 1405 89
AlwNI CAG_nnn'CTG
Cuts at: 0 34 611 863 926 1031 1494
Size: 34 577 252 63 105 463
Fragments arranged by size:
577 463 252 105 63 34
ApaI G_GGCC'C
Cuts at: 0 101 1494
Size: 101 1393
ApoI r'AATT_y
Cuts at: 0 890 1270 1494
Size: 890 380 224
Fragments arranged by size:
890 380 224
AvaI C'yCGr_G
Cuts at: 0 101 1494
Size: 101 1393
BamHI G'GATC_C
Cuts at: 0 106 1494
Size: 106 1388
BanI G'GyrC_C
Cuts at: 0 158 1494
Size: 158 1336
BanII G_rGCy'C
Cuts at: 0 101 700 1288 1372 1494
Size: 101 599 588 84 122
Fragments arranged by size:
599 588 122 101 84
BbsI GAAGACnn'nnnn_
Cuts at: 0 311 1494
Size: 311 1183
BciVI GTATCCnnnnn_n'
Cuts at: 0 191 1494
Size: 191 1303
BfrBI ATG'CAT
Cuts at: 0 580 1494
Size: 580 914
BlpI GC'TnA_GC
Cuts at: 0 867 1287 1494
Size: 867 420 207
Fragments arranged by size:
867 420 207
Bme1580I G_kGCm'C
Cuts at: 0 101 491 1494
Size: 101 390 1003
Fragments arranged by size:
1003 390 101
BmrI ACTGGGnnnn_n'
Cuts at: 0 258 1160 1494
Size: 258 902 334
Fragments arranged by size:
902 334 258
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 719 1494
Size: 719 775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 308 316 668 752 1494
Size: 25 283 8 352 84 742
Fragments arranged by size:
742 352 283 84 25 8
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 1494
Size: 460 1034
BsaI GGTCTCn'nnnn_
Cuts at: 0 88 453 495 1494
Size: 88 365 42 999
Fragments arranged by size:
999 365 88 42
BsaWI w'CCGG_w
Cuts at: 0 814 898 1318 1445 1494
Size: 814 84 420 127 49
Fragments arranged by size:
814 420 127 84 49
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 76 1494
Size: 76 1418
BseYI C'CCAG_C
Cuts at: 0 94 245 1494
Size: 94 151 1249
Fragments arranged by size:
1249 151 94
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 687 771 1023 1191 1470 1494
Size: 687 84 252 168 279 24
Fragments arranged by size:
687 279 252 168 84 24
BsiHKAI G_wGCw'C
Cuts at: 0 700 1288 1372 1494
Size: 700 588 84 122
Fragments arranged by size:
700 588 122 84
BsmI GAATG_Cn'
Cuts at: 0 786 922 1426 1494
Size: 786 136 504 68
Fragments arranged by size:
786 504 136 68
Bsp1286I G_dGCh'C
Cuts at: 0 101 491 700 1288 1372 1494
Size: 101 390 209 588 84 122
Fragments arranged by size:
588 390 209 122 101 84
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 317 1494
Size: 317 1177
BssSI C'ACGA_G
Cuts at: 0 163 1494
Size: 163 1331
BstYI r'GATC_y
Cuts at: 0 106 1494
Size: 106 1388
BtgI C'CryG_G
Cuts at: 0 175 423 491 1494
Size: 175 248 68 1003
Fragments arranged by size:
1003 248 175 68
BtsI GCAGTG_nn'
Cuts at: 0 129 675 759 1494
Size: 129 546 84 735
Fragments arranged by size:
735 546 129 84
EarI CTCTTCn'nnn_
Cuts at: 0 439 631 759 1494
Size: 439 192 128 735
Fragments arranged by size:
735 439 192 128
EcoICRI GAG'CTC
Cuts at: 0 698 1286 1370 1494
Size: 698 588 84 124
Fragments arranged by size:
698 588 124 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 102 308 316 392 588 668 672
Size: 25 77 206 8 76 196 80 4
Cuts at: 672 752 756 840 1008 1092 1176 1260 1494
Size: 80 4 84 168 84 84 84 234
Fragments arranged by size:
234 206 196 168 84 84 84 84
80 80 77 76 25 8 4 4
EcoNI CCTnn'n_nnAGG
Cuts at: 0 26 1494
Size: 26 1468
EcoO109I rG'GnC_Cy
Cuts at: 0 31 98 1494
Size: 31 67 1396
Fragments arranged by size:
1396 67 31
EcoRI G'AATT_C
Cuts at: 0 890 1494
Size: 890 604
FspI TGC'GCA
Cuts at: 0 1035 1494
Size: 1035 459
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 438 452 470 484 1191 1223 1494
Size: 438 14 18 14 707 32 271
Fragments arranged by size:
707 438 271 32 18 14 14
HindIII A'AGCT_T
Cuts at: 0 374 1494
Size: 374 1120
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 248 1494
Size: 248 1246
MslI CAynn'nnrTG
Cuts at: 0 579 1251 1405 1494
Size: 579 672 154 89
Fragments arranged by size:
672 579 154 89
NdeI CA'TA_TG
Cuts at: 0 576 1494
Size: 576 918
NsiI A_TGCA'T
Cuts at: 0 582 1494
Size: 582 912
NspI r_CATG'y
Cuts at: 0 584 1494
Size: 584 910
PasI CC'CwG_GG
Cuts at: 0 409 1494
Size: 409 1085
PfoI T'CCnGG_A
Cuts at: 0 211 1494
Size: 211 1283
PspOMI G'GGCC_C
Cuts at: 0 97 1494
Size: 97 1397
SacI G_AGCT'C
Cuts at: 0 700 1288 1372 1494
Size: 700 588 84 122
Fragments arranged by size:
700 588 122 84
SapI GCTCTTCn'nnn_
Cuts at: 0 439 631 759 1494
Size: 439 192 128 735
Fragments arranged by size:
735 439 192 128
ScaI AGT'ACT
Cuts at: 0 17 1494
Size: 17 1477
SfcI C'TryA_G
Cuts at: 0 828 1080 1494
Size: 828 252 414
Fragments arranged by size:
828 414 252
SmaI CCC'GGG
Cuts at: 0 103 1494
Size: 103 1391
SmlI C'TyrA_G
Cuts at: 0 439 1494
Size: 439 1055
StuI AGG'CCT
Cuts at: 0 685 937 1021 1105 1189 1441 1494
Size: 685 252 84 84 84 252 53
Fragments arranged by size:
685 252 252 84 84 84 53
TatI w'GTAC_w
Cuts at: 0 15 1494
Size: 15 1479
XmaI C'CCGG_G
Cuts at: 0 101 1494
Size: 101 1393
XmnI GAAnn'nnTTC
Cuts at: 0 448 685 853 937 1021 1105 1189 1273
Size: 448 237 168 84 84 84 84 84
Cuts at: 1273 1441 1494
Size: 168 53
Fragments arranged by size:
448 237 168 168 84 84 84 84
84 53
Enzymes that do cut:
AccI AcuI AleI AlwNI ApaI ApoI AvaI BamHI
BanI BanII BbsI BciVI BfrBI BlpI Bme1580I BmrI
BplI BpmI BpuEI BsaI BsaWI BseRI BseYI BsgI
BsiHKAI BsmI Bsp1286I BspMI BssSI BstYI BtgI BtsI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI Hin4I
HindIII MmeI MslI NdeI NsiI NspI PasI PfoI
PspOMI SacI SapI ScaI SfcI SmaI SmlI StuI
TatI XmaI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AlfI AloI ApaLI AscI AseI AsiSI AvrII
BaeI BbeI BbvCI BcgI BclI BglI BglII BmgBI
BmtI Bpu10I BsaAI BsaBI BsaHI BsaXI BsiEI BsiWI
BsmBI BspEI BspHI BsrBI BsrDI BsrFI BsrGI BssHII
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EciI EcoRV
FalI FseI FspAI HaeII HincII HpaI KasI KpnI
MfeI MluI MscI MspA1I NaeI NarI NcoI NgoMIV
NheI NotI NruI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SbfI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StyI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
ZraI