Restriction map for
pFN21AB7755
(Circular) (Six-base) MAPSORT of: pFN21AB7755.seq Check: 2684 from: 1 to: 6043
pFN21AB7755 6043 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2215 2391 2215
Size: 176 5867
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2850 3853 278
Size: 53 83 186 936 1314 1003 2468
Fragments arranged by size:
2468 1314 1003 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3386 1937
Size: 1449 4594
Acc65I G'GTAC_C
Cuts at: 3364 3364
Size: 6043
AclI AA'CG_TT
Cuts at: 1563 4170 4543 1563
Size: 2607 373 3063
Fragments arranged by size:
3063 2607 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2637 2756 2770 4106 5490 5758 1632
Size: 1005 119 14 1336 1384 268 1917
Fragments arranged by size:
1917 1384 1336 1005 268 119 14
AfeI AGC'GCT
Cuts at: 2192 2192
Size: 6043
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5846
AflIII A'CryG_T
Cuts at: 1203 4942 5919 1203
Size: 3739 977 1327
Fragments arranged by size:
3739 1327 977
AgeI A'CCGG_T
Cuts at: 4874 4874
Size: 6043
AhdI GACnn_n'nnGTC
Cuts at: 2218 4772 2218
Size: 2554 3489
AleI CACnn'nnGTG
Cuts at: 1166 2961 1166
Size: 1795 4248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6043
AlwNI CAG_nnn'CTG
Cuts at: 2869 3306 3786 5358 2869
Size: 437 480 1572 3554
Fragments arranged by size:
3554 1572 480 437
ApaI G_GGCC'C
Cuts at: 2956 2956
Size: 6043
ApaLI G'TGCA_C
Cuts at: 4100 5256 4100
Size: 1156 4887
ApoI r'AATT_y
Cuts at: 1493 3352 3578 3730 5839 1493
Size: 1859 226 152 2109 1697
Fragments arranged by size:
2109 1859 1697 226 152
AscI GG'CGCG_CC
Cuts at: 2351 2351
Size: 6043
AseI AT'TA_AT
Cuts at: 160 1032 4597 160
Size: 872 3565 1606
Fragments arranged by size:
3565 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
AvaI C'yCGr_G
Cuts at: 1870 1943 2160 2566 2894 3060 3368 1870
Size: 73 217 406 328 166 308 4545
Fragments arranged by size:
4545 406 328 308 217 166 73
BamHI G'GATC_C
Cuts at: 3373 3740 3373
Size: 367 5676
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2054 2156 2254 2600 2637
Size: 325 217 627 267 102 98 346 37
Cuts at: 2637 2720 2769 2828 2918 3182 3364 4819 618
Size: 83 49 59 90 264 182 1455 1842
Fragments arranged by size:
1842 1455 627 346 325 267 264 217
182 102 98 90 83 59 49 37
BanII G_rGCy'C
Cuts at: 1391 2400 2811 2956 3038 3362 1391
Size: 1009 411 145 82 324 4072
Fragments arranged by size:
4072 1009 411 324 145 82
BbeI G_GCGC'C
Cuts at: 2160 2724 2773 2922 2160
Size: 564 49 149 5281
Fragments arranged by size:
5281 564 149 49
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5072
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 4234 4268 1903
Size: 34 50 34 2213 34 3678
Fragments arranged by size:
3678 2213 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3935 5145 3935
Size: 1210 4833
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6043
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2052 2972 3048
Size: 107 122 71 631 605 380 920 76
Cuts at: 3048 3110 3241 4654 136
Size: 62 131 1413 1525
Fragments arranged by size:
1525 1413 920 631 605 380 131 122
107 76 71 62
BglII A'GATC_T
Cuts at: 6038 6038
Size: 6043
BlpI GC'TnA_GC
Cuts at: 2399 2432 2810 3460 2399
Size: 33 378 650 4982
Fragments arranged by size:
4982 650 378 33
Bme1580I G_kGCm'C
Cuts at: 1790 2259 2640 2831 2956 2968 4104 5260 1790
Size: 469 381 191 125 12 1136 1156 2573
Fragments arranged by size:
2573 1156 1136 469 381 191 125 12
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6043
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3316 3774 4732
Size: 416 53 83 399 24 1899 458 958
Cuts at: 4732 442
Size: 1753
Fragments arranged by size:
1899 1753 958 458 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6043
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2044 2044
Size: 6043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2266 2762 3154 4703 1239
Size: 1027 496 392 1549 2579
Fragments arranged by size:
2579 1549 1027 496 392
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2216 4168 5033 5331 2216
Size: 1952 865 298 2928
Fragments arranged by size:
2928 1952 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4706 882
Size: 617 3207 2219
Fragments arranged by size:
3207 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2077 3834 493
Size: 711 873 1757 2702
Fragments arranged by size:
2702 1757 873 711
BsaBI GATnn'nnATC
Cuts at: 6037 6037
Size: 6043
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2157 2214 2721 2749
Size: 53 83 186 936 624 57 507 28
Cuts at: 2749 2770 2838 2847 2919 2939 3850 4232 4867
Size: 21 68 9 72 20 911 382 635
Cuts at: 4867 275
Size: 1451
Fragments arranged by size:
1451 936 911 635 624 507 382 186
83 72 68 57 53 28 21 20
9
BsaWI w'CCGG_w
Cuts at: 4476 4874 5148 5295 5706 4476
Size: 398 274 147 411 4813
Fragments arranged by size:
4813 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2543 2573 1178
Size: 30 165 30 1140 30 4648
Fragments arranged by size:
4648 1140 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2477 1186
Size: 1291 4752
BseYI C'CCAG_C
Cuts at: 2031 2410 2578 5246 5742 2031
Size: 379 168 2668 496 2332
Fragments arranged by size:
2668 2332 496 379 168
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2369 2504 1163
Size: 573 633 135 4702
Fragments arranged by size:
4702 633 573 135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2756 3011 3114 3493 4254 5282
Size: 275 186 755 255 103 379 761 1028
Cuts at: 5282 1540
Size: 2301
Fragments arranged by size:
2301 1028 761 755 379 275 255 186
103
BsiHKAI G_wGCw'C
Cuts at: 2232 2400 2811 4104 4189 5260 2232
Size: 168 411 1293 85 1071 3015
Fragments arranged by size:
3015 1293 1071 411 168 85
BsmI GAATG_Cn'
Cuts at: 2327 3554 3647 2327
Size: 1227 93 4723
Fragments arranged by size:
4723 1227 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2112 2987 2112
Size: 875 5168
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2232 2259 2400 2640 2811 2831 2956
Size: 399 442 27 141 240 171 20 125
Cuts at: 2956 2968 3038 3362 4104 4189 5260 1391
Size: 12 70 324 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 442 399 324 240 171
141 125 85 70 27 20 12
BspEI T'CCGG_A
Cuts at: 5706 5706
Size: 6043
BspHI T'CATG_A
Cuts at: 3932 5662 3932
Size: 1730 4313
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2215 2391 2877 3168 3398 844
Size: 1371 176 486 291 230 3489
Fragments arranged by size:
3489 1371 486 291 230 176
BsrBI CCG'CTC
Cuts at: 1394 1823 2028 3930 1394
Size: 429 205 1902 3507
Fragments arranged by size:
3507 1902 429 205
BsrDI GCAATG_nn'
Cuts at: 65 1248 4538 4712 65
Size: 1183 3290 174 1396
Fragments arranged by size:
3290 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2114 2263 2546 2751 2755 3122 3134
Size: 110 304 149 283 205 4 367 12
Cuts at: 3134 3240 3316 4687 4874 1700
Size: 106 76 1371 187 2869
Fragments arranged by size:
2869 1371 367 304 283 205 187 149
110 106 76 12 4
BsrGI T'GTAC_A
Cuts at: 96 2607 3321 96
Size: 2511 714 2818
Fragments arranged by size:
2818 2511 714
BssHII G'CGCG_C
Cuts at: 1925 2286 2351 1925
Size: 361 65 5617
Fragments arranged by size:
5617 361 65
BssSI C'ACGA_G
Cuts at: 2852 4103 5115 2852
Size: 1251 1012 3780
Fragments arranged by size:
3780 1251 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6043
BstBI TT'CG_AA
Cuts at: 4847 4847
Size: 6043
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6043
BstYI r'GATC_y
Cuts at: 1972 3373 3740 4125 4142 5594 5680 5692 6038
Size: 1401 367 385 17 1452 86 12 346
Cuts at: 6038 1972
Size: 1977
Fragments arranged by size:
1977 1452 1401 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2522 2644 2668 2832 3005 513
Size: 552 1457 122 24 164 173 3551
Fragments arranged by size:
3551 1457 552 173 164 122 24
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5304
Fragments arranged by size:
5304 683 56
BtsI GCAGTG_nn'
Cuts at: 3559 4353 4373 3559
Size: 794 20 5229
Fragments arranged by size:
5229 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6043
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6008
DraI TTT'AAA
Cuts at: 3347 3347
Size: 6043
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5050 809
Size: 507 3734 1802
Fragments arranged by size:
3734 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2364 2548 2753
Size: 54 1425 50 275 18 534 184 205
Cuts at: 2753 3008 3040 3091 3111 3490 4379 6008 8
Size: 255 32 51 20 379 889 1629 43
Fragments arranged by size:
1629 1425 889 534 379 275 255 205
184 54 51 50 43 32 20 18
EagI C'GGCC_G
Cuts at: 1537 1812 2753 3008 3111 3490 1537
Size: 275 941 255 103 379 4090
Fragments arranged by size:
4090 941 379 275 255 103
EarI CTCTTCn'nnn_
Cuts at: 1353 2528 3973 1353
Size: 1175 1445 3423
Fragments arranged by size:
3423 1445 1175
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2189 2198 4630 5004 5150 387
Size: 1064 138 600 9 2432 374 146 1280
Fragments arranged by size:
2432 1280 1064 600 374 146 138 9
EcoICRI GAG'CTC
Cuts at: 2398 2809 2398
Size: 411 5632
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2266 2637 2756 2762 2770 3154 4106
Size: 393 634 371 119 6 8 384 952
Cuts at: 4106 4703 5490 5758 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 952 787 634 597 393 384 371
268 119 8 6
EcoO109I rG'GnC_Cy
Cuts at: 2125 2582 2999 2125
Size: 457 417 5169
Fragments arranged by size:
5169 457 417
EcoRI G'AATT_C
Cuts at: 3352 3730 3352
Size: 378 5665
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2470 2642 2470
Size: 172 5871
FspI TGC'GCA
Cuts at: 4549 4549
Size: 6043
HaeII r_GCGC'y
Cuts at: 1678 2160 2194 2428 2476 2541 2724 2773 2922
Size: 482 34 234 48 65 183 49 149
Cuts at: 2922 3105 5190 1678
Size: 183 2085 2531
Fragments arranged by size:
2531 2085 482 234 183 183 149 65
49 48 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2075 2107 2204 2236 2549
Size: 32 135 32 503 32 97 32 313
Cuts at: 2549 2581 4684 4716 4758 4790 1373
Size: 32 2103 32 42 32 2626
Fragments arranged by size:
2626 2103 503 313 135 97 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3387 3633 669
Size: 988 281 1449 246 3079
Fragments arranged by size:
3079 1449 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6043
HpaI GTT'AAC
Cuts at: 3633 3633
Size: 6043
KasI G'GCGC_C
Cuts at: 2156 2720 2769 2918 2156
Size: 564 49 149 5281
Fragments arranged by size:
5281 564 149 49
KpnI G_GTAC'C
Cuts at: 3368 3368
Size: 6043
MfeI C'AATT_G
Cuts at: 3642 3642
Size: 6043
MluI A'CGCG_T
Cuts at: 5919 5919
Size: 6043
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2586 5157 5341 1305
Size: 1281 2571 184 2007
Fragments arranged by size:
2571 2007 1281 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4221
Fragments arranged by size:
4221 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2264 2643 2961 4001 4360 4519 518
Size: 648 1098 379 318 1040 359 159 2042
Fragments arranged by size:
2042 1098 1040 648 379 359 318 159
MspA1I CmG'CkG
Cuts at: 1606 2766 3090 3459 4136 5284 5529 1606
Size: 1160 324 369 677 1148 245 2120
Fragments arranged by size:
2120 1160 1148 677 369 324 245
NaeI GCC'GGC
Cuts at: 2116 2548 2753 3124 3136 3242 2116
Size: 432 205 371 12 106 4917
Fragments arranged by size:
4917 432 371 205 106 12
NarI GG'CG_CC
Cuts at: 2157 2721 2770 2919 2157
Size: 564 49 149 5281
Fragments arranged by size:
5281 564 149 49
NcoI C'CATG_G
Cuts at: 513 1065 2644 2832 513
Size: 552 1579 188 3724
Fragments arranged by size:
3724 1579 552 188
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6043
NgoMIV G'CCGG_C
Cuts at: 2114 2546 2751 3122 3134 3240 2114
Size: 432 205 371 12 106 4917
Fragments arranged by size:
4917 432 371 205 106 12
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6043
NotI GC'GGCC_GC
Cuts at: 3490 3490
Size: 6043
NspI r_CATG'y
Cuts at: 1132 1229 2309 3401 4946 1132
Size: 97 1080 1092 1545 2229
Fragments arranged by size:
2229 1545 1092 1080 97
PasI CC'CwG_GG
Cuts at: 1348 2875 1348
Size: 1527 4516
PciI A'CATG_T
Cuts at: 4942 4942
Size: 6043
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3306 1108
Size: 2198 3845
PmeI GTTT'AAAC
Cuts at: 3347 3347
Size: 6043
PmlI CAC'GTG
Cuts at: 2077 2077
Size: 6043
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4112 4144 5625 5657 4112
Size: 32 1481 32 4498
Fragments arranged by size:
4498 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2582 2999 2582
Size: 417 5626
PshAI GACnn'nnGTC
Cuts at: 1613 2247 1613
Size: 634 5409
PsiI TTA'TAA
Cuts at: 3613 3613
Size: 6043
PspOMI G'GGCC_C
Cuts at: 2952 2952
Size: 6043
PstI C_TGCA'G
Cuts at: 830 2045 2712 3089 3165 3395 830
Size: 1215 667 377 76 230 3478
Fragments arranged by size:
3478 1215 667 377 230 76
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6043
SacI G_AGCT'C
Cuts at: 2400 2811 2400
Size: 411 5632
SalI G'TCGA_C
Cuts at: 1936 3385 1936
Size: 1449 4594
SanDI GG'GwC_CC
Cuts at: 2582 2999 2582
Size: 417 5626
SapI GCTCTTCn'nnn_
Cuts at: 2528 2528
Size: 6043
SbfI CC_TGCA'GG
Cuts at: 3165 3395 3165
Size: 230 5813
ScaI AGT'ACT
Cuts at: 4291 6003 4291
Size: 1712 4331
SfcI C'TryA_G
Cuts at: 826 1045 2041 2708 3085 3161 3391 4526 5207
Size: 219 996 667 377 76 230 1135 681
Cuts at: 5207 5398 826
Size: 191 1471
Fragments arranged by size:
1471 1135 996 681 667 377 230 219
191 76
SfoI GGC'GCC
Cuts at: 2158 2722 2771 2920 2158
Size: 564 49 149 5281
Fragments arranged by size:
5281 564 149 49
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6043
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6043
SmaI CCC'GGG
Cuts at: 1872 2162 3370 1872
Size: 290 1208 4545
Fragments arranged by size:
4545 1208 290
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2231 4147 5048 5310 820
Size: 197 926 288 1916 901 262 1553
Fragments arranged by size:
1916 1553 926 901 288 262 197
SnaBI TAC'GTA
Cuts at: 493 3834 493
Size: 3341 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6043
SphI G_CATG'C
Cuts at: 1132 3401 1132
Size: 2269 3774
SspI AAT'ATT
Cuts at: 5 52 3967 5
Size: 47 3915 2081
Fragments arranged by size:
3915 2081 47
StuI AGG'CCT
Cuts at: 1517 2909 3167 1517
Size: 1392 258 4393
Fragments arranged by size:
4393 1392 258
StyI C'CwwG_G
Cuts at: 513 1065 2644 2832 3482 513
Size: 552 1579 188 650 3074
Fragments arranged by size:
3074 1579 650 552 188
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 4070 4087 4240 4425 4870 325
Size: 1255 2490 17 153 185 445 1498
Fragments arranged by size:
2490 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2607 3321 4289
Size: 274 80 33 51 1443 630 714 968
Cuts at: 4289 6001 96
Size: 1712 138
Fragments arranged by size:
1712 1443 968 714 630 274 138 80
51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2543 2575 6003 6035 2543
Size: 32 3428 32 2551
Fragments arranged by size:
3428 2551 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6043
XbaI T'CTAG_A
Cuts at: 3379 3379
Size: 6043
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2074 2074
Size: 6043
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6043
XmaI C'CCGG_G
Cuts at: 1870 2160 3368 1870
Size: 290 1208 4545
Fragments arranged by size:
4545 1208 290
XmnI GAAnn'nnTTC
Cuts at: 2327 4172 2327
Size: 1845 4198
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2848 3851 276
Size: 53 83 186 936 1314 1003 2468
Fragments arranged by size:
2468 1314 1003 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PstI PvuI SacI SalI SanDI SapI
SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AloI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstXI
BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI
NsiI PacI PfoI PspXI PsrI PvuII RsrII SacII
SexAI SfiI SrfI SwaI