(Circular) (Six-base) MAPSORT of: pFN21AB7749.seq Check: 5035 from: 1 to: 6034 pFN21AB7749 6034 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3844 278 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3377 1937 Size: 1440 4594 Acc65I G'GTAC_C Cuts at: 2912 3355 2912 Size: 443 5591 AclI AA'CG_TT Cuts at: 1563 4161 4534 1563 Size: 2598 373 3063 Fragments arranged by size: 3063 2598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4097 5481 5749 1632 Size: 2465 1384 268 1917 Fragments arranged by size: 2465 1917 1384 268 AfeI AGC'GCT Cuts at: 2987 2987 Size: 6034 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5837 AflIII A'CryG_T Cuts at: 1203 4933 5910 1203 Size: 3730 977 1327 Fragments arranged by size: 3730 1327 977 AgeI A'CCGG_T Cuts at: 4865 4865 Size: 6034 AhdI GACnn_n'nnGTC Cuts at: 4763 4763 Size: 6034 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6034 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2783 2825 1184 Size: 1599 42 4393 Fragments arranged by size: 4393 1599 42 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2615 2647 2615 Size: 32 6002 AlwNI CAG_nnn'CTG Cuts at: 3777 5349 3777 Size: 1572 4462 ApaLI G'TGCA_C Cuts at: 4091 5247 4091 Size: 1156 4878 ApoI r'AATT_y Cuts at: 1493 3303 3343 3569 3721 5830 1493 Size: 1810 40 226 152 2109 1697 Fragments arranged by size: 2109 1810 1697 226 152 40 AseI AT'TA_AT Cuts at: 160 1032 4588 160 Size: 872 3556 1606 Fragments arranged by size: 3556 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 AvaI C'yCGr_G Cuts at: 1870 1943 3181 3359 1870 Size: 73 1238 178 4545 Fragments arranged by size: 4545 1238 178 73 BamHI G'GATC_C Cuts at: 3364 3731 3364 Size: 367 5667 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2123 2912 3017 3185 3203 Size: 325 217 627 336 789 105 168 18 Cuts at: 3203 3355 4810 618 Size: 152 1455 1842 Fragments arranged by size: 1842 1455 789 627 336 325 217 168 152 105 18 BanII G_rGCy'C Cuts at: 1391 2435 2558 2877 3126 3353 1391 Size: 1044 123 319 249 227 4072 Fragments arranged by size: 4072 1044 319 249 227 123 BbeI G_GCGC'C Cuts at: 2127 3189 2127 Size: 1062 4972 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3091 928 Size: 971 1192 3871 Fragments arranged by size: 3871 1192 971 BbvCI CC'TCA_GC Cuts at: 2319 2331 2319 Size: 12 6022 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2133 2167 4225 4259 1903 Size: 34 50 34 112 34 2058 34 3678 Fragments arranged by size: 3678 2058 112 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3926 5136 3926 Size: 1210 4824 BclI T'GATC_A Cuts at: 1548 1548 Size: 6034 BfrBI ATG'CAT Cuts at: 2845 2845 Size: 6034 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2423 3054 4645 Size: 107 122 71 631 605 751 631 1591 Cuts at: 4645 136 Size: 1525 Fragments arranged by size: 1591 1525 751 631 631 605 122 107 71 BglII A'GATC_T Cuts at: 2712 6029 2712 Size: 3317 2717 BlpI GC'TnA_GC Cuts at: 3451 3451 Size: 6034 Bme1580I G_kGCm'C Cuts at: 1790 2814 3020 4095 5251 1790 Size: 1024 206 1075 1156 2573 Fragments arranged by size: 2573 1156 1075 1024 206 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6034 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2872 3765 4723 Size: 416 53 83 399 24 1455 893 958 Cuts at: 4723 442 Size: 1753 Fragments arranged by size: 1753 1455 958 893 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6034 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2144 2155 2506 2642 4694 1239 Size: 905 11 351 136 2052 2579 Fragments arranged by size: 2579 2052 905 351 136 11 Bpu10I CC'TnA_GC Cuts at: 2228 2319 2331 2527 2228 Size: 91 12 196 5735 Fragments arranged by size: 5735 196 91 12 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4159 5024 5322 4159 Size: 865 298 4871 Fragments arranged by size: 4871 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4697 882 Size: 617 3198 2219 Fragments arranged by size: 3198 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3825 493 Size: 711 2621 2702 Fragments arranged by size: 2702 2621 711 BsaBI GATnn'nnATC Cuts at: 2711 6028 2711 Size: 3317 2717 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2108 2124 3186 3841 Size: 53 83 186 936 575 16 1062 655 Cuts at: 3841 4223 4858 275 Size: 382 635 1451 Fragments arranged by size: 1451 1062 936 655 635 575 382 186 83 53 16 BsaWI w'CCGG_w Cuts at: 4467 4865 5139 5286 5697 4467 Size: 398 274 147 411 4804 Fragments arranged by size: 4804 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2615 2645 1178 Size: 30 165 30 1212 30 4567 Fragments arranged by size: 4567 1212 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2376 1186 Size: 1190 4844 BseYI C'CCAG_C Cuts at: 2954 3214 5237 5733 2954 Size: 260 2023 496 3255 Fragments arranged by size: 3255 2023 496 260 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2258 2325 1163 Size: 573 522 67 4872 Fragments arranged by size: 4872 573 522 67 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2660 3484 4245 5273 1540 Size: 275 186 659 824 761 1028 2301 Fragments arranged by size: 2301 1028 824 761 659 275 186 BsiHKAI G_wGCw'C Cuts at: 4095 4180 5251 4095 Size: 85 1071 4878 Fragments arranged by size: 4878 1071 85 BsmI GAATG_Cn' Cuts at: 2398 2845 3545 3638 2398 Size: 447 700 93 4794 Fragments arranged by size: 4794 700 447 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2435 2558 2814 2877 3020 3126 3353 Size: 399 645 123 256 63 143 106 227 Cuts at: 3353 4095 4180 5251 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 645 399 256 227 143 123 106 85 63 BspEI T'CCGG_A Cuts at: 5697 5697 Size: 6034 BspHI T'CATG_A Cuts at: 3923 5653 3923 Size: 1730 4304 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3389 844 Size: 2545 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3921 1394 Size: 429 2098 3507 Fragments arranged by size: 3507 2098 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4529 4703 65 Size: 1183 3281 174 1396 Fragments arranged by size: 3281 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2655 2822 3278 4678 4865 1700 Size: 110 845 167 456 1400 187 2869 Fragments arranged by size: 2869 1400 845 456 187 167 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6034 BssHII G'CGCG_C Cuts at: 1925 2581 2583 2585 2604 1925 Size: 656 2 2 19 5355 Fragments arranged by size: 5355 656 19 2 2 BssSI C'ACGA_G Cuts at: 2599 4094 5106 2599 Size: 1495 1012 3527 Fragments arranged by size: 3527 1495 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6034 BstBI TT'CG_AA Cuts at: 4838 4838 Size: 6034 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6034 BstYI r'GATC_y Cuts at: 1972 2712 3364 3731 4116 4133 5585 5671 5683 Size: 740 652 367 385 17 1452 86 12 Cuts at: 5683 6029 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 740 652 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3189 3232 513 Size: 552 2124 43 3315 Fragments arranged by size: 3315 2124 552 43 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5295 Fragments arranged by size: 5295 683 56 BtsI GCAGTG_nn' Cuts at: 3550 4344 4364 3550 Size: 794 20 5220 Fragments arranged by size: 5220 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6034 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5999 DraI TTT'AAA Cuts at: 3338 3338 Size: 6034 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5041 809 Size: 507 3725 1802 Fragments arranged by size: 3725 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2424 2457 2657 Size: 54 1425 50 275 18 594 33 200 Cuts at: 2657 2676 3223 3280 3481 4370 5999 8 Size: 19 547 57 201 889 1629 43 Fragments arranged by size: 1629 1425 889 594 547 275 201 200 57 54 50 43 33 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2657 3481 1537 Size: 275 845 824 4090 Fragments arranged by size: 4090 845 824 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2377 3964 1353 Size: 1024 1587 3423 Fragments arranged by size: 3423 1587 1024 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2101 4621 4995 5141 387 Size: 1064 138 512 2520 374 146 1280 Fragments arranged by size: 2520 1280 1064 512 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2144 2155 2506 2642 4097 4694 5481 Size: 393 512 11 351 136 1455 597 787 Cuts at: 5481 5749 1239 Size: 268 1524 Fragments arranged by size: 1524 1455 787 597 512 393 351 268 136 11 EcoO109I rG'GnC_Cy Cuts at: 3072 3242 3072 Size: 170 5864 EcoRI G'AATT_C Cuts at: 3303 3343 3721 3303 Size: 40 378 5616 Fragments arranged by size: 5616 378 40 FspI TGC'GCA Cuts at: 2222 2962 4540 2222 Size: 740 1578 3716 Fragments arranged by size: 3716 1578 740 HaeII r_GCGC'y Cuts at: 1678 2127 2178 2235 2704 2989 3189 5181 1678 Size: 449 51 57 469 285 200 1992 2531 Fragments arranged by size: 2531 1992 469 449 285 200 57 51 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2665 2697 2864 2896 4675 Size: 32 135 32 1093 32 167 32 1779 Cuts at: 4675 4707 4749 4781 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1779 1093 167 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2857 3378 3624 669 Size: 988 281 919 521 246 3079 Fragments arranged by size: 3079 988 919 521 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6034 HpaI GTT'AAC Cuts at: 3624 3624 Size: 6034 KasI G'GCGC_C Cuts at: 2123 3185 2123 Size: 1062 4972 KpnI G_GTAC'C Cuts at: 2916 3359 2916 Size: 443 5591 MfeI C'AATT_G Cuts at: 3633 3633 Size: 6034 MluI A'CGCG_T Cuts at: 5910 5910 Size: 6034 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5148 5332 1305 Size: 3843 184 2007 Fragments arranged by size: 3843 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 2678 10 Size: 54 1425 343 627 219 3366 Fragments arranged by size: 3366 1425 627 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 3042 3992 4351 4510 518 Size: 648 1876 950 359 159 2042 Fragments arranged by size: 2042 1876 950 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2611 2958 3214 3222 3450 4127 5275 5520 Size: 1005 347 256 8 228 677 1148 245 Cuts at: 5520 1606 Size: 2120 Fragments arranged by size: 2120 1148 1005 677 347 256 245 228 8 NaeI GCC'GGC Cuts at: 2657 2657 Size: 6034 NarI GG'CG_CC Cuts at: 2124 3186 2124 Size: 1062 4972 NcoI C'CATG_G Cuts at: 513 1065 3189 513 Size: 552 2124 3358 Fragments arranged by size: 3358 2124 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6034 NgoMIV G'CCGG_C Cuts at: 2655 2655 Size: 6034 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6034 NotI GC'GGCC_GC Cuts at: 3481 3481 Size: 6034 NsiI A_TGCA'T Cuts at: 2847 2847 Size: 6034 NspI r_CATG'y Cuts at: 1132 1229 2770 3392 4937 1132 Size: 97 1541 622 1545 2229 Fragments arranged by size: 2229 1545 1541 622 97 PasI CC'CwG_GG Cuts at: 1348 2908 1348 Size: 1560 4474 PciI A'CATG_T Cuts at: 4933 4933 Size: 6034 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6034 PmeI GTTT'AAAC Cuts at: 3338 3338 Size: 6034 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2615 2647 4103 4135 5616 5648 2615 Size: 32 1456 32 1481 32 3001 Fragments arranged by size: 3001 1481 1456 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3072 3072 Size: 6034 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6034 PsiI TTA'TAA Cuts at: 2082 3604 2082 Size: 1522 4512 PstI C_TGCA'G Cuts at: 830 3054 3386 830 Size: 2224 332 3478 Fragments arranged by size: 3478 2224 332 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6034 PvuII CAG'CTG Cuts at: 2611 2958 2611 Size: 347 5687 SalI G'TCGA_C Cuts at: 1936 3376 1936 Size: 1440 4594 SanDI GG'GwC_CC Cuts at: 3072 3072 Size: 6034 SbfI CC_TGCA'GG Cuts at: 3054 3386 3054 Size: 332 5702 ScaI AGT'ACT Cuts at: 4282 5994 4282 Size: 1712 4322 SexAI A'CCwGG_T Cuts at: 3330 3330 Size: 6034 SfcI C'TryA_G Cuts at: 826 1045 3050 3382 4517 5198 5389 826 Size: 219 2005 332 1135 681 191 1471 Fragments arranged by size: 2005 1471 1135 681 332 219 191 SfoI GGC'GCC Cuts at: 2125 3187 2125 Size: 1062 4972 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6034 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6034 SmaI CCC'GGG Cuts at: 1872 3361 1872 Size: 1489 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4138 5039 5301 820 Size: 197 926 2195 901 262 1553 Fragments arranged by size: 2195 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3825 493 Size: 3332 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6034 SphI G_CATG'C Cuts at: 1132 2770 3392 1132 Size: 1638 622 3774 Fragments arranged by size: 3774 1638 622 SspI AAT'ATT Cuts at: 5 52 3958 5 Size: 47 3906 2081 Fragments arranged by size: 3906 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6034 StyI C'CwwG_G Cuts at: 513 1065 2190 3189 3473 513 Size: 552 1125 999 284 3074 Fragments arranged by size: 3074 1125 999 552 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2464 3331 3341 4061 4078 4231 4416 Size: 1255 884 867 10 720 17 153 185 Cuts at: 4416 4861 325 Size: 445 1498 Fragments arranged by size: 1498 1255 884 867 720 445 185 153 17 10 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2739 4280 5992 Size: 274 80 33 51 1443 762 1541 1712 Cuts at: 5992 96 Size: 138 Fragments arranged by size: 1712 1541 1443 762 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5994 6026 5994 Size: 32 6002 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6034 XbaI T'CTAG_A Cuts at: 3370 3370 Size: 6034 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6034 XmaI C'CCGG_G Cuts at: 1870 3359 1870 Size: 1489 4545 XmnI GAAnn'nnTTC Cuts at: 2630 4163 2630 Size: 1533 4501 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3842 276 Size: 53 83 186 936 2308 2468 Fragments arranged by size: 2468 2308 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI ApaI AscI AvrII BaeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SrfI SwaI XcmI