Restriction map for
pFN21AB7744
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AocI ApaLI ApoI
AscI AseI AsnI Asp718I AspEI
BanI BbeI BbsI BcgI BciVI
BfrI BglII BpuAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BspLU11I BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI Hin1I HincII HindII
HindIII HpaI KasI KpnI KspI
MamI MfeI MluI MroI MseI
MslI MunI NarI NcoI NdeI
NotI NruI NsiI NspV PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tsp509I
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# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 1342 CGCG CG^CG
Aor51HI 1223 AGCGCT AGC^GCT
ApaI 969 GGGCCC GGGCC^C
Asp700I 1194 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 564 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 677 TGCGCA TGC^GCA
AvrII 733 CCTAGG C^CTAGG
BalI 600 TGGCCA TGG^CCA
BamHI 881 GGATCC G^GATCC
BbrPI 636 CACGTG CAC^GTG
BlnI 733 CCTAGG C^CTAGG
BsaAI 636 CACGTG [CT]AC^GT[GA]
BsaI 1106 GGTCTC GGTCTC(1/5)
BsgI 304 GTGCAG GTGCAG(16/14)
BsmI 818 GCATTC GAATGC(1/-1)
BspHI 1047 TCATGA T^CATGA
BsrFI 1303 GCCGGC [GA]^CCGG[CT]
BssSI 741 CTCGTG CACGAG(-5/-1)
BstUI 1342 CGCG CG^CG
BstXI 290 CCAGGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1303 GCCGGC [GA]^CCGG[CT]
DraIII 80 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1223 AGCGCT AGC^GCT
FspI 677 TGCGCA TGC^GCA
HaeII 1223 AGCGCT [GA]GCGC^[CT]
MaeII 637 ACGT A^CGT
MluNI 600 TGGCCA TGG^CCA
MscI 600 TGGCCA TGG^CCA
MvnI 1342 CGCG CG^CG
NaeI 1303 GCCGGC GCC^GGC
NgoMIV 1303 GCCGGC G^CCGGC
PflFI 564 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 636 CACGTG CAC^GTG
PmlI 636 CACGTG CAC^GTG
PpuMI 428 GGGACCC [GA]G^G[AT]CC[CT]
PstI 253 CTGCAG CTGCA^G
PvuII 157 CAGCTG CAG^CTG
RcaI 1047 TCATGA T^CATGA
SexAI 565 ACCAGGT A^CC[AT]GGT
SmaI 1229 CCCGGG CCC^GGG
SphI 322 GCATGC GCATG^C
StuI 366 AGGCCT AGG^CCT
TfiI 408 GAATC G^A[AT]TC
Tth111I 564 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 463 TCTAGA T^CTAGA
XcmI 588 CCAAGCCCCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1229 CCCGGG C^CCGGG
XmaI 1229 CCCGGG C^CCGGG
XmnI 1194 GAAGCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AfaI 52 GTAC GT^AC
1281 GTAC
AspHI 631 GTGCTC G[AT]GC[AT]^C
1122 GTGCTC
AvaII 429 GGACC G^G[AT]CC
1376 GGACC
BbvCI 1219 CCTCAGC CCTCAGC(-5/-2)
748 GCTGAGG
BclI 95 TGATCA T^GATCA
1008 TGATCA
BlpI 550 GCTCAGC GC^T[ACGT]AGC
981 GCTCAGC
Bpu1102I 550 GCTCAGC GC^T[ACGT]AGC
981 GCTCAGC
BsiHKAI 631 GTGCTC G[AT]GC[AT]^C
1122 GTGCTC
BsmBI 1415 CGTCTC CGTCTC(1/5)
1165 GAGACG
BspMI 1256 ACCTGC ACCTGC(4/8)
142 GCAGGT
BsrBI 1274 CCGCTC CCGCTC(-3/-3)
1383 CCGCTC
BstAPI 624 GCATGGAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1033 GCAACCTGTGC
BstYI 360 AGATCC [GA]^GATC[CT]
881 GGATCC
CelII 550 GCTCAGC GC^T[ACGT]AGC
981 GCTCAGC
EarI 316 GAAGAG CTCTTC(1/4)
1000 GAAGAG
FbaI 95 TGATCA T^GATCA
1008 TGATCA
HgaI 1167 GACGC GACGC(5/10)
725 GCGTC
HphI 98 TCACC GGTGA(8/7)
728 TCACC
Ksp632I 316 GAAGAG CTCTTC(1/4)
1000 GAAGAG
MaeIII 727 GTCAC ^GT[ACGT]AC
1346 GTCAC
MflI 360 AGATCC [GA]^GATC[CT]
881 GGATCC
MspA1I 157 CAGCTG C[AC]G^C[GT]G
1284 CCGCTG
NheI 57 GCTAGC G^CTAGC
138 GCTAGC
NspI 322 GCATGC [GA]CATG^[CT]
1337 ACATGC
PaeR7I 335 CTCGAG C^TCGAG
1407 CTCGAG
PflMI 126 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
153 CCAGCAGCTGG
PleI 1003 GAGTC GAGTC(4/5)
1178 GAGTC
RsaI 52 GTAC GT^AC
1281 GTAC
SfcI 253 CTGCAG C^T[GA][CT]AG
1206 CTGTAG
SfiI 163 GGCCGCAGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1412 GGCCGTCTCGGCC
Tsp45I 727 GTCAC ^GT[GC]AC
1346 GTCAC
Van91I 126 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
153 CCAGCAGCTGG
XhoI 335 CTCGAG C^TCGAG
1407 CTCGAG
XhoII 360 AGATCC [GA]^GATC[CT]
881 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
MaeI MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
SmlI StyI TaqI TseI TspRI
TthHB8I