(Circular) (Six-base) MAPSORT of: pFN21AB7744.seq Check: 847 from: 1 to: 6166 pFN21AB7744 6166 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2138 3270 2138 Size: 1132 5034 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3976 278 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3509 1937 Size: 1572 4594 Acc65I G'GTAC_C Cuts at: 3487 3487 Size: 6166 AclI AA'CG_TT Cuts at: 1563 4293 4666 1563 Size: 2730 373 3063 Fragments arranged by size: 3063 2730 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2267 2396 4229 5613 5881 1632 Size: 635 129 1833 1384 268 1917 Fragments arranged by size: 1917 1833 1384 635 268 129 AfeI AGC'GCT Cuts at: 3230 3230 Size: 6166 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5969 AflIII A'CryG_T Cuts at: 1203 5065 6042 1203 Size: 3862 977 1327 Fragments arranged by size: 3862 1327 977 AgeI A'CCGG_T Cuts at: 4997 4997 Size: 6166 AhdI GACnn_n'nnGTC Cuts at: 4895 4895 Size: 6166 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6166 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6166 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2871 2903 2871 Size: 32 6134 AlwNI CAG_nnn'CTG Cuts at: 2164 2227 2317 3909 5481 2164 Size: 63 90 1592 1572 2849 Fragments arranged by size: 2849 1592 1572 90 63 ApaI G_GGCC'C Cuts at: 2978 2978 Size: 6166 ApaLI G'TGCA_C Cuts at: 4223 5379 4223 Size: 1156 5010 ApoI r'AATT_y Cuts at: 1493 3475 3701 3853 5962 1493 Size: 1982 226 152 2109 1697 Fragments arranged by size: 2109 1982 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4720 160 Size: 872 3688 1606 Fragments arranged by size: 3688 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 AvaI C'yCGr_G Cuts at: 1870 1943 2340 2528 3234 3412 3491 1870 Size: 73 397 188 706 178 79 4545 Fragments arranged by size: 4545 706 397 188 178 79 73 AvrII C'CTAG_G Cuts at: 2738 2738 Size: 6166 BamHI G'GATC_C Cuts at: 2886 3496 3863 2886 Size: 610 367 5189 Fragments arranged by size: 5189 610 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3487 4942 618 Size: 325 217 627 1700 1455 1842 Fragments arranged by size: 1842 1700 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2188 2341 2669 2919 2978 3485 1391 Size: 797 153 328 250 59 507 4072 Fragments arranged by size: 4072 797 507 328 250 153 59 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5195 BbvCI CC'TCA_GC Cuts at: 2754 3225 2754 Size: 471 5695 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4357 4391 1903 Size: 34 2420 34 3678 Fragments arranged by size: 3678 2420 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4058 5268 4058 Size: 1210 4956 BclI T'GATC_A Cuts at: 1548 2100 3013 1548 Size: 552 913 4701 Fragments arranged by size: 4701 913 552 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2040 2175 3424 Size: 107 122 71 631 605 368 135 1249 Cuts at: 3424 4777 136 Size: 1353 1525 Fragments arranged by size: 1525 1353 1249 631 605 368 135 122 107 71 BglII A'GATC_T Cuts at: 6161 6161 Size: 6166 BlpI GC'TnA_GC Cuts at: 2556 2987 3583 2556 Size: 431 596 5139 Fragments arranged by size: 5139 596 431 Bme1580I G_kGCm'C Cuts at: 1790 2978 3024 4227 5383 1790 Size: 1188 46 1203 1156 2573 Fragments arranged by size: 2573 1203 1188 1156 46 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6166 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2438 3897 4855 Size: 416 53 83 399 24 1021 1459 958 Cuts at: 4855 442 Size: 1753 Fragments arranged by size: 1753 1459 1021 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2066 2147 1056 Size: 1010 81 5075 Fragments arranged by size: 5075 1010 81 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2422 3438 2422 Size: 1016 5150 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2286 2353 2460 2874 2929 2941 4826 1239 Size: 1047 67 107 414 55 12 1885 2579 Fragments arranged by size: 2579 1885 1047 414 107 67 55 12 Bpu10I CC'TnA_GC Cuts at: 2273 2754 3225 2273 Size: 481 471 5214 Fragments arranged by size: 5214 481 471 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2392 3140 4291 5156 5454 2392 Size: 748 1151 865 298 3104 Fragments arranged by size: 3104 1151 865 748 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3117 4829 882 Size: 617 1618 1712 2219 Fragments arranged by size: 2219 1712 1618 617 BsaAI yAC'GTr Cuts at: 493 1204 2643 3957 493 Size: 711 1439 1314 2702 Fragments arranged by size: 2702 1439 1314 711 BsaBI GATnn'nnATC Cuts at: 6160 6160 Size: 6166 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3973 4355 4990 275 Size: 53 83 186 936 2440 382 635 1451 Fragments arranged by size: 2440 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4599 4997 5271 5418 5829 4599 Size: 398 274 147 411 4936 Fragments arranged by size: 4936 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3278 3308 1178 Size: 30 165 30 1875 30 4036 Fragments arranged by size: 4036 1875 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2405 2932 3397 3400 1186 Size: 1219 527 465 3 3952 Fragments arranged by size: 3952 1219 527 465 3 BseYI C'CCAG_C Cuts at: 2030 2164 2220 2707 3317 3359 5369 5865 2030 Size: 134 56 487 610 42 2010 496 2331 Fragments arranged by size: 2331 2010 610 496 487 134 56 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2330 1163 Size: 573 594 4999 Fragments arranged by size: 4999 594 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3616 4377 5405 1540 Size: 275 186 1615 761 1028 2301 Fragments arranged by size: 2301 1615 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2640 3131 4227 4312 5383 2640 Size: 491 1096 85 1071 3423 Fragments arranged by size: 3423 1096 1071 491 85 BsmI GAATG_Cn' Cuts at: 2823 3677 3770 2823 Size: 854 93 5219 Fragments arranged by size: 5219 854 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3164 3426 3164 Size: 262 5904 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2188 2341 2640 2669 2919 2978 3024 Size: 399 398 153 299 29 250 59 46 Cuts at: 3024 3131 3485 4227 4312 5383 1391 Size: 107 354 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 398 354 299 250 153 107 85 59 46 29 BspEI T'CCGG_A Cuts at: 5829 5829 Size: 6166 BspHI T'CATG_A Cuts at: 3052 4055 5785 3052 Size: 1003 1730 3433 Fragments arranged by size: 3433 1730 1003 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2138 3270 3521 844 Size: 1294 1132 251 3489 Fragments arranged by size: 3489 1294 1132 251 BsrBI CCG'CTC Cuts at: 1394 1823 3281 3390 4053 1394 Size: 429 1458 109 663 3507 Fragments arranged by size: 3507 1458 663 429 109 BsrDI GCAATG_nn' Cuts at: 65 1248 4661 4835 65 Size: 1183 3413 174 1396 Fragments arranged by size: 3413 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3308 4810 4997 1700 Size: 110 1498 1502 187 2869 Fragments arranged by size: 2869 1502 1498 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6166 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6166 BssSI C'ACGA_G Cuts at: 2746 4226 5238 2746 Size: 1480 1012 3674 Fragments arranged by size: 3674 1480 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2635 3044 794 Size: 1841 409 3916 Fragments arranged by size: 3916 1841 409 BstBI TT'CG_AA Cuts at: 4970 4970 Size: 6166 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6166 BstXI CCAn_nnnn'nTGG Cuts at: 2302 2302 Size: 6166 BstYI r'GATC_y Cuts at: 1972 2365 2886 3496 3863 4248 4265 5717 5803 Size: 393 521 610 367 385 17 1452 86 Cuts at: 5803 5815 6161 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 610 521 393 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5614 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5427 Fragments arranged by size: 5427 683 56 BtsI GCAGTG_nn' Cuts at: 3682 4476 4496 3682 Size: 794 20 5352 Fragments arranged by size: 5352 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6166 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6131 DraI TTT'AAA Cuts at: 3470 3470 Size: 6166 DraIII CAC_nnn'GTG Cuts at: 2090 2090 Size: 6166 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5173 809 Size: 507 3857 1802 Fragments arranged by size: 3857 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2078 2292 2605 Size: 54 1425 50 275 18 248 214 313 Cuts at: 2605 3246 3613 4502 6131 8 Size: 641 367 889 1629 43 Fragments arranged by size: 1629 1425 889 641 367 313 275 248 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3613 1537 Size: 275 1801 4090 Fragments arranged by size: 4090 1801 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2316 3000 4096 1353 Size: 963 684 1096 3423 Fragments arranged by size: 3423 1096 963 684 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4753 5127 5273 387 Size: 1064 138 3164 374 146 1280 Fragments arranged by size: 3164 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2267 2286 2353 2396 2460 2874 2929 Size: 393 635 19 67 43 64 414 55 Cuts at: 2929 2941 4229 4826 5613 5881 1239 Size: 12 1288 597 787 268 1524 Fragments arranged by size: 1524 1288 787 635 597 414 393 268 67 64 55 43 19 12 EcoNI CCTnn'n_nnAGG Cuts at: 2285 2321 2955 2969 3412 2285 Size: 36 634 14 443 5039 Fragments arranged by size: 5039 634 443 36 14 EcoO109I rG'GnC_Cy Cuts at: 2277 2298 2434 2742 2758 2974 2975 2277 Size: 21 136 308 16 216 1 5468 Fragments arranged by size: 5468 308 216 136 21 16 1 EcoRI G'AATT_C Cuts at: 3475 3853 3475 Size: 378 5788 FspI TGC'GCA Cuts at: 2684 4672 2684 Size: 1988 4178 HaeII r_GCGC'y Cuts at: 1678 3232 5313 1678 Size: 1554 2081 2531 Fragments arranged by size: 2531 2081 1554 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2184 2216 2641 2673 3369 Size: 32 135 32 612 32 425 32 696 Cuts at: 3369 3401 4807 4839 4881 4913 1373 Size: 32 1406 32 42 32 2626 Fragments arranged by size: 2626 1406 696 612 425 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3510 3756 669 Size: 988 281 1572 246 3079 Fragments arranged by size: 3079 1572 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6166 HpaI GTT'AAC Cuts at: 3756 3756 Size: 6166 KpnI G_GTAC'C Cuts at: 3491 3491 Size: 6166 MfeI C'AATT_G Cuts at: 3765 3765 Size: 6166 MluI A'CGCG_T Cuts at: 6042 6042 Size: 6166 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5280 5464 1305 Size: 3975 184 2007 Fragments arranged by size: 3975 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2607 10 Size: 54 1425 343 775 3569 Fragments arranged by size: 3569 1425 775 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4124 4483 4642 518 Size: 648 2958 359 159 2042 Fragments arranged by size: 2958 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2164 3291 3582 4259 5407 5652 1606 Size: 558 1127 291 677 1148 245 2120 Fragments arranged by size: 2120 1148 1127 677 558 291 245 NaeI GCC'GGC Cuts at: 3310 3310 Size: 6166 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5614 NdeI CA'TA_TG Cuts at: 387 387 Size: 6166 NgoMIV G'CCGG_C Cuts at: 3308 3308 Size: 6166 NheI G'CTAG_C Cuts at: 1052 2062 2143 1052 Size: 1010 81 5075 Fragments arranged by size: 5075 1010 81 NotI GC'GGCC_GC Cuts at: 3613 3613 Size: 6166 NspI r_CATG'y Cuts at: 1132 1229 2331 3346 3524 5069 1132 Size: 97 1102 1015 178 1545 2229 Fragments arranged by size: 2229 1545 1102 1015 178 97 PasI CC'CwG_GG Cuts at: 1348 2971 2993 1348 Size: 1623 22 4521 Fragments arranged by size: 4521 1623 22 PciI A'CATG_T Cuts at: 5065 5065 Size: 6166 PflMI CCAn_nnn'nTGG Cuts at: 1108 2137 2164 1108 Size: 1029 27 5110 Fragments arranged by size: 5110 1029 27 PmeI GTTT'AAAC Cuts at: 3470 3470 Size: 6166 PmlI CAC'GTG Cuts at: 2643 2643 Size: 6166 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4235 4267 5748 5780 4235 Size: 32 1481 32 4621 Fragments arranged by size: 4621 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2434 2434 Size: 6166 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6166 PsiI TTA'TAA Cuts at: 3736 3736 Size: 6166 PspOMI G'GGCC_C Cuts at: 2974 2974 Size: 6166 PspXI vC'TCGA_Gb Cuts at: 2340 3412 2340 Size: 1072 5094 PstI C_TGCA'G Cuts at: 830 2262 3518 830 Size: 1432 1256 3478 Fragments arranged by size: 3478 1432 1256 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6166 PvuII CAG'CTG Cuts at: 2164 2164 Size: 6166 SalI G'TCGA_C Cuts at: 1936 3508 1936 Size: 1572 4594 SanDI GG'GwC_CC Cuts at: 2434 2434 Size: 6166 SbfI CC_TGCA'GG Cuts at: 3518 3518 Size: 6166 ScaI AGT'ACT Cuts at: 4414 6126 4414 Size: 1712 4454 SexAI A'CCwGG_T Cuts at: 2570 2570 Size: 6166 SfcI C'TryA_G Cuts at: 826 1045 2258 3211 3514 4649 5330 5521 826 Size: 219 1213 953 303 1135 681 191 1471 Fragments arranged by size: 1471 1213 1135 953 681 303 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2175 3424 2175 Size: 1249 4917 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6166 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6166 SmaI CCC'GGG Cuts at: 1872 3236 3493 1872 Size: 1364 257 4545 Fragments arranged by size: 4545 1364 257 SmlI C'TyrA_G Cuts at: 820 1017 1943 2340 2407 3155 3412 4270 5171 Size: 197 926 397 67 748 257 858 901 Cuts at: 5171 5433 820 Size: 262 1553 Fragments arranged by size: 1553 926 901 858 748 397 262 257 197 67 SnaBI TAC'GTA Cuts at: 493 3957 493 Size: 3464 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6166 SphI G_CATG'C Cuts at: 1132 2331 3524 1132 Size: 1199 1193 3774 Fragments arranged by size: 3774 1199 1193 SspI AAT'ATT Cuts at: 5 52 4090 5 Size: 47 4038 2081 Fragments arranged by size: 4038 2081 47 StuI AGG'CCT Cuts at: 1517 2373 1517 Size: 856 5310 StyI C'CwwG_G Cuts at: 513 1065 2301 2738 2762 3605 513 Size: 552 1236 437 24 843 3074 Fragments arranged by size: 3074 1236 843 552 437 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3099 4193 4210 4363 4548 4993 325 Size: 1255 1519 1094 17 153 185 445 1498 Fragments arranged by size: 1519 1498 1255 1094 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4412 6124 96 Size: 274 80 33 51 1443 2435 1712 138 Fragments arranged by size: 2435 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6126 6158 6126 Size: 32 6134 Tth111I GACn'n_nGTC Cuts at: 1316 2572 1316 Size: 1256 4910 XbaI T'CTAG_A Cuts at: 2468 3502 2468 Size: 1034 5132 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2600 2600 Size: 6166 XhoI C'TCGA_G Cuts at: 1943 2340 3412 1943 Size: 397 1072 4697 Fragments arranged by size: 4697 1072 397 XmaI C'CCGG_G Cuts at: 1870 3234 3491 1870 Size: 1364 257 4545 Fragments arranged by size: 4545 1364 257 XmnI GAAnn'nnTTC Cuts at: 3203 4295 3203 Size: 1092 5074 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3974 276 Size: 53 83 186 936 2440 2468 Fragments arranged by size: 2468 2440 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PfoI PsrI RsrII SacI SacII SapI SfoI SrfI SwaI