Restriction map for
pFN21AB7744
(Linear) (Six-base) MAPSORT of: KIBB7744.seq Check: 2903 from: 1 to: 1461
KIBB7744.orf
With 182 enzymes: SgfI *
March 3, 2008 11:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 133 1265 1461
Size: 133 1132 196
Fragments arranged by size:
1132 196 133
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 391 1461
Size: 262 129 1070
Fragments arranged by size:
1070 262 129
AfeI AGC'GCT
Cuts at: 0 1225 1461
Size: 1225 236
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 866 898 1461
Size: 866 32 563
Fragments arranged by size:
866 563 32
AlwNI CAG_nnn'CTG
Cuts at: 0 159 222 312 1461
Size: 159 63 90 1149
Fragments arranged by size:
1149 159 90 63
ApaI G_GGCC'C
Cuts at: 0 973 1461
Size: 973 488
AvaI C'yCGr_G
Cuts at: 0 335 523 1229 1407 1461
Size: 335 188 706 178 54
Fragments arranged by size:
706 335 188 178 54
AvrII C'CTAG_G
Cuts at: 0 733 1461
Size: 733 728
BamHI G'GATC_C
Cuts at: 0 881 1461
Size: 881 580
BanII G_rGCy'C
Cuts at: 0 183 336 664 914 973 1461
Size: 183 153 328 250 59 488
Fragments arranged by size:
488 328 250 183 153 59
BbvCI CC'TCA_GC
Cuts at: 0 749 1220 1461
Size: 749 471 241
Fragments arranged by size:
749 471 241
BclI T'GATC_A
Cuts at: 0 95 1008 1461
Size: 95 913 453
Fragments arranged by size:
913 453 95
BglI GCCn_nnn'nGGC
Cuts at: 0 35 170 1419 1461
Size: 35 135 1249 42
Fragments arranged by size:
1249 135 42 35
BlpI GC'TnA_GC
Cuts at: 0 551 982 1461
Size: 551 431 479
Fragments arranged by size:
551 479 431
Bme1580I G_kGCm'C
Cuts at: 0 973 1019 1461
Size: 973 46 442
Fragments arranged by size:
973 442 46
BmrI ACTGGGnnnn_n'
Cuts at: 0 433 1461
Size: 433 1028
BmtI G_CTAG'C
Cuts at: 0 61 142 1461
Size: 61 81 1319
Fragments arranged by size:
1319 81 61
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 417 1433 1461
Size: 417 1016 28
Fragments arranged by size:
1016 417 28
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 281 348 455 869 924 936 1461
Size: 281 67 107 414 55 12 525
Fragments arranged by size:
525 414 281 107 67 55 12
Bpu10I CC'TnA_GC
Cuts at: 0 268 749 1220 1461
Size: 268 481 471 241
Fragments arranged by size:
481 471 268 241
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 387 1135 1461
Size: 387 748 326
Fragments arranged by size:
748 387 326
BsaI GGTCTCn'nnnn_
Cuts at: 0 1112 1461
Size: 1112 349
BsaAI yAC'GTr
Cuts at: 0 638 1461
Size: 638 823
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1273 1303 1461
Size: 1273 30 158
Fragments arranged by size:
1273 158 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 927 1392 1395 1461
Size: 400 527 465 3 66
Fragments arranged by size:
527 465 400 66 3
BseYI C'CCAG_C
Cuts at: 0 25 159 215 702 1312 1354 1461
Size: 25 134 56 487 610 42 107
Fragments arranged by size:
610 487 134 107 56 42 25
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 325 1461
Size: 325 1136
BsiHKAI G_wGCw'C
Cuts at: 0 635 1126 1461
Size: 635 491 335
Fragments arranged by size:
635 491 335
BsmI GAATG_Cn'
Cuts at: 0 818 1461
Size: 818 643
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1159 1421 1461
Size: 1159 262 40
Fragments arranged by size:
1159 262 40
Bsp1286I G_dGCh'C
Cuts at: 0 183 336 635 664 914 973 1019 1126
Size: 183 153 299 29 250 59 46 107
Cuts at: 1126 1461
Size: 335
Fragments arranged by size:
335 299 250 183 153 107 59 46
29
BspHI T'CATG_A
Cuts at: 0 1047 1461
Size: 1047 414
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 133 1265 1461
Size: 133 1132 196
Fragments arranged by size:
1132 196 133
BsrBI CCG'CTC
Cuts at: 0 1276 1385 1461
Size: 1276 109 76
Fragments arranged by size:
1276 109 76
BsrFI r'CCGG_y
Cuts at: 0 1303 1461
Size: 1303 158
BssSI C'ACGA_G
Cuts at: 0 741 1461
Size: 741 720
BstAPI GCAn_nnn'nTGC
Cuts at: 0 630 1039 1461
Size: 630 409 422
Fragments arranged by size:
630 422 409
BstXI CCAn_nnnn'nTGG
Cuts at: 0 297 1461
Size: 297 1164
BstYI r'GATC_y
Cuts at: 0 360 881 1461
Size: 360 521 580
Fragments arranged by size:
580 521 360
DraIII CAC_nnn'GTG
Cuts at: 0 85 1461
Size: 85 1376
EaeI y'GGCC_r
Cuts at: 0 73 287 600 1241 1461
Size: 73 214 313 641 220
Fragments arranged by size:
641 313 220 214 73
EarI CTCTTCn'nnn_
Cuts at: 0 311 995 1461
Size: 311 684 466
Fragments arranged by size:
684 466 311
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 262 281 348 391 455 869 924 936
Size: 262 19 67 43 64 414 55 12
Cuts at: 936 1461
Size: 525
Fragments arranged by size:
525 414 262 67 64 55 43 19
12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 280 316 950 964 1407 1461
Size: 280 36 634 14 443 54
Fragments arranged by size:
634 443 280 54 36 14
EcoO109I rG'GnC_Cy
Cuts at: 0 272 293 429 737 753 969 970 1461
Size: 272 21 136 308 16 216 1 491
Fragments arranged by size:
491 308 272 216 136 21 16 1
FspI TGC'GCA
Cuts at: 0 679 1461
Size: 679 782
HaeII r_GCGC'y
Cuts at: 0 1227 1461
Size: 1227 234
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 179 211 636 668 1364 1396 1461
Size: 179 32 425 32 696 32 65
Fragments arranged by size:
696 425 179 65 32 32 32
MscI TGG'CCA
Cuts at: 0 602 1461
Size: 602 859
MspA1I CmG'CkG
Cuts at: 0 159 1286 1461
Size: 159 1127 175
Fragments arranged by size:
1127 175 159
NaeI GCC'GGC
Cuts at: 0 1305 1461
Size: 1305 156
NgoMIV G'CCGG_C
Cuts at: 0 1303 1461
Size: 1303 158
NheI G'CTAG_C
Cuts at: 0 57 138 1461
Size: 57 81 1323
Fragments arranged by size:
1323 81 57
NspI r_CATG'y
Cuts at: 0 326 1341 1461
Size: 326 1015 120
Fragments arranged by size:
1015 326 120
PasI CC'CwG_GG
Cuts at: 0 966 988 1461
Size: 966 22 473
Fragments arranged by size:
966 473 22
PflMI CCAn_nnn'nTGG
Cuts at: 0 132 159 1461
Size: 132 27 1302
Fragments arranged by size:
1302 132 27
PmlI CAC'GTG
Cuts at: 0 638 1461
Size: 638 823
PpuMI rG'GwC_Cy
Cuts at: 0 429 1461
Size: 429 1032
PspOMI G'GGCC_C
Cuts at: 0 969 1461
Size: 969 492
PspXI vC'TCGA_Gb
Cuts at: 0 335 1407 1461
Size: 335 1072 54
Fragments arranged by size:
1072 335 54
PstI C_TGCA'G
Cuts at: 0 257 1461
Size: 257 1204
PvuII CAG'CTG
Cuts at: 0 159 1461
Size: 159 1302
SanDI GG'GwC_CC
Cuts at: 0 429 1461
Size: 429 1032
SexAI A'CCwGG_T
Cuts at: 0 565 1461
Size: 565 896
SfcI C'TryA_G
Cuts at: 0 253 1206 1461
Size: 253 953 255
Fragments arranged by size:
953 255 253
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 170 1419 1461
Size: 170 1249 42
Fragments arranged by size:
1249 170 42
SmaI CCC'GGG
Cuts at: 0 1231 1461
Size: 1231 230
SmlI C'TyrA_G
Cuts at: 0 335 402 1150 1407 1461
Size: 335 67 748 257 54
Fragments arranged by size:
748 335 257 67 54
SphI G_CATG'C
Cuts at: 0 326 1461
Size: 326 1135
StuI AGG'CCT
Cuts at: 0 368 1461
Size: 368 1093
StyI C'CwwG_G
Cuts at: 0 296 733 757 1461
Size: 296 437 24 704
Fragments arranged by size:
704 437 296 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1094 1461
Size: 1094 367
Tth111I GACn'n_nGTC
Cuts at: 0 567 1461
Size: 567 894
XbaI T'CTAG_A
Cuts at: 0 463 1461
Size: 463 998
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 595 1461
Size: 595 866
XhoI C'TCGA_G
Cuts at: 0 335 1407 1461
Size: 335 1072 54
Fragments arranged by size:
1072 335 54
XmaI C'CCGG_G
Cuts at: 0 1229 1461
Size: 1229 232
XmnI GAAnn'nnTTC
Cuts at: 0 1198 1461
Size: 1198 263
Enzymes that do cut:
AarI AcuI AfeI AloI AlwNI ApaI AvaI AvrII
BamHI BanII BbvCI BclI BglI BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI DraIII
EaeI EarI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I
MscI MspA1I NaeI NgoMIV NheI NspI PasI PflMI
PmlI PpuMI PspOMI PspXI PstI PvuII SanDI SexAI
SfcI SfiI SmaI SmlI SphI StuI StyI TaqII
Tth111I XbaI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AflII AflIII AgeI AhdI
AleI AlfI ApaLI ApoI AscI AseI AsiSI BaeI
BanI BbeI BbsI BcgI BciVI BfrBI BglII BmgBI
BsaBI BsaHI BsaWI BsiEI BsiWI BspEI BsrDI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspAI HincII HindIII HpaI KasI
KpnI MfeI MluI MmeI MslI NarI NcoI NdeI
NotI NruI NsiI PacI PciI PfoI PmeI PpiI
PshAI PsiI PsrI PvuI RsrII SacI SacII SalI
SapI SbfI ScaI SfoI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI TatI TstI ZraI