(Circular) (Six-base) MAPSORT of: pFN21AB7741.seq Check: 7183 from: 1 to: 6013 pFN21AB7741 6013 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3356 1937 Size: 1419 4594 Acc65I G'GTAC_C Cuts at: 3334 3334 Size: 6013 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2098 4076 5460 5728 1632 Size: 466 1978 1384 268 1917 Fragments arranged by size: 1978 1917 1384 466 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 4912 5889 1203 Size: 3709 977 1327 Fragments arranged by size: 3709 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AlwNI CAG_nnn'CTG Cuts at: 3756 5328 3756 Size: 1572 4441 ApaLI G'TGCA_C Cuts at: 2472 4070 5226 2472 Size: 1598 1156 3259 Fragments arranged by size: 3259 1598 1156 ApoI r'AATT_y Cuts at: 1493 2727 3322 3548 3700 5809 1493 Size: 1234 595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1234 595 226 152 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 3338 1870 Size: 73 1395 4545 Fragments arranged by size: 4545 1395 73 BamHI G'GATC_C Cuts at: 3343 3710 3343 Size: 367 5646 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3334 4789 618 Size: 325 217 627 1547 1455 1842 Fragments arranged by size: 1842 1547 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2374 2998 3305 3332 1391 Size: 983 624 307 27 4072 Fragments arranged by size: 4072 983 624 307 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2326 3073 3157 928 Size: 971 427 747 84 3784 Fragments arranged by size: 3784 971 747 427 84 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4204 4238 1903 Size: 34 2267 34 3678 Fragments arranged by size: 3678 2267 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3905 5115 3905 Size: 1210 4803 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BfrBI ATG'CAT Cuts at: 2659 2659 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2825 6008 2825 Size: 3183 2830 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2476 4074 5230 1790 Size: 686 1598 1156 2573 Fragments arranged by size: 2573 1598 1156 686 BmgBI CAC'GTC Cuts at: 1318 2973 1318 Size: 1655 4358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2457 2709 3048 Size: 416 53 83 399 24 1040 252 339 Cuts at: 3048 3744 4702 442 Size: 696 958 1753 Fragments arranged by size: 1753 1040 958 696 416 399 339 252 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2945 2945 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2316 2685 2769 2858 3024 3192 4673 1239 Size: 1077 369 84 89 166 168 1481 2579 Fragments arranged by size: 2579 1481 1077 369 168 166 89 84 Bpu10I CC'TnA_GC Cuts at: 3299 3299 Size: 6013 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4138 5003 5301 4138 Size: 865 298 4850 Fragments arranged by size: 4850 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2497 4676 882 Size: 617 998 2179 2219 Fragments arranged by size: 2219 2179 998 617 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2420 3820 4202 4837 Size: 53 83 186 936 887 1400 382 635 Cuts at: 4837 275 Size: 1451 Fragments arranged by size: 1451 1400 936 887 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4446 4844 5118 5265 5676 4446 Size: 398 274 147 411 4783 Fragments arranged by size: 4783 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5788 Fragments arranged by size: 5788 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2066 1186 Size: 880 5133 BseYI C'CCAG_C Cuts at: 5216 5712 5216 Size: 496 5517 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3157 1163 Size: 573 1421 4019 Fragments arranged by size: 4019 1421 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2133 3463 4224 5252 1540 Size: 275 186 132 1330 761 1028 2301 Fragments arranged by size: 2301 1330 1028 761 275 186 132 BsiHKAI G_wGCw'C Cuts at: 2374 2476 2998 4074 4159 5230 2374 Size: 102 522 1076 85 1071 3157 Fragments arranged by size: 3157 1076 1071 522 102 85 BsmI GAATG_Cn' Cuts at: 2519 3524 3617 2519 Size: 1005 93 4915 Fragments arranged by size: 4915 1005 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2411 2411 Size: 6013 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2374 2476 2998 3305 3332 4074 4159 Size: 399 584 102 522 307 27 742 85 Cuts at: 4159 5230 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 584 522 399 307 102 85 27 BspEI T'CCGG_A Cuts at: 5676 5676 Size: 6013 BspHI T'CATG_A Cuts at: 3238 3902 5632 3238 Size: 664 1730 3619 Fragments arranged by size: 3619 1730 664 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2789 3368 844 Size: 1945 579 3489 Fragments arranged by size: 3489 1945 579 BsrBI CCG'CTC Cuts at: 1394 1823 3900 1394 Size: 429 2077 3507 Fragments arranged by size: 3507 2077 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4844 1700 Size: 110 2847 187 2869 Fragments arranged by size: 2869 2847 187 110 BsrGI T'GTAC_A Cuts at: 96 3107 96 Size: 3011 3002 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2523 794 Size: 1729 4284 BstBI TT'CG_AA Cuts at: 2641 3064 4817 2641 Size: 423 1753 3837 Fragments arranged by size: 3837 1753 423 BstEII G'GTnAC_C Cuts at: 1184 2801 1184 Size: 1617 4396 BstXI CCAn_nnnn'nTGG Cuts at: 3275 3275 Size: 6013 BstYI r'GATC_y Cuts at: 1972 2825 3343 3710 4095 4112 5564 5650 5662 Size: 853 518 367 385 17 1452 86 12 Cuts at: 5662 6008 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 853 518 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4522 Fragments arranged by size: 4522 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5274 Fragments arranged by size: 5274 683 56 BtsI GCAGTG_nn' Cuts at: 3529 4323 4343 3529 Size: 794 20 5199 Fragments arranged by size: 5199 794 20 ClaI AT'CG_AT Cuts at: 1554 2706 1554 Size: 1152 4861 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5978 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3255 3460 4349 Size: 54 1425 50 275 18 1425 205 889 Cuts at: 4349 5978 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1425 889 275 205 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3943 1353 Size: 2590 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4600 4974 5120 387 Size: 1064 138 3011 374 146 1280 Fragments arranged by size: 3011 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2372 2996 2372 Size: 624 5389 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2098 2316 2685 2769 2858 3024 3192 Size: 393 466 218 369 84 89 166 168 Cuts at: 3192 4076 4673 5460 5728 1239 Size: 884 597 787 268 1524 Fragments arranged by size: 1524 884 787 597 466 393 369 268 218 168 166 89 84 EcoO109I rG'GnC_Cy Cuts at: 2589 2589 Size: 6013 EcoRI G'AATT_C Cuts at: 3322 3700 3322 Size: 378 5635 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4654 4686 4728 4760 1373 Size: 32 135 32 3082 32 42 32 2626 Fragments arranged by size: 3082 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3338 3338 Size: 6013 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2113 5127 5311 1305 Size: 808 3014 184 2007 Fragments arranged by size: 3014 2007 808 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4191 Fragments arranged by size: 4191 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2311 2901 3103 3971 4330 4489 518 Size: 648 1145 590 202 868 359 159 2042 Fragments arranged by size: 2042 1145 868 648 590 359 202 159 MspA1I CmG'CkG Cuts at: 1606 3429 4106 5254 5499 1606 Size: 1823 677 1148 245 2120 Fragments arranged by size: 2120 1823 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4522 Fragments arranged by size: 4522 939 552 NdeI CA'TA_TG Cuts at: 387 3100 387 Size: 2713 3300 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NsiI A_TGCA'T Cuts at: 2661 2661 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 3371 4916 1132 Size: 97 2142 1545 2229 Fragments arranged by size: 2229 2142 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6013 PciI A'CATG_T Cuts at: 4912 4912 Size: 6013 PflMI CCAn_nnn'nTGG Cuts at: 1108 2179 1108 Size: 1071 4942 PfoI T'CCnGG_A Cuts at: 2352 2352 Size: 6013 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2111 2143 4082 4114 5595 5627 2111 Size: 32 1939 32 1481 32 2497 Fragments arranged by size: 2497 1939 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2589 2589 Size: 6013 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PstI C_TGCA'G Cuts at: 830 3365 830 Size: 2535 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SacI G_AGCT'C Cuts at: 2374 2998 2374 Size: 624 5389 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SfcI C'TryA_G Cuts at: 826 1045 2089 3361 4496 5177 5368 826 Size: 219 1044 1272 1135 681 191 1471 Fragments arranged by size: 1471 1272 1135 1044 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4117 5018 5280 820 Size: 197 926 2174 901 262 1553 Fragments arranged by size: 2174 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6013 StyI C'CwwG_G Cuts at: 513 1065 2004 3452 513 Size: 552 939 1448 3074 Fragments arranged by size: 3074 1448 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2060 2119 3120 4040 4057 4210 4395 Size: 1255 480 59 1001 920 17 153 185 Cuts at: 4395 4840 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1001 920 480 445 185 153 59 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3107 4259 5971 Size: 274 80 33 51 1443 1130 1152 1712 Cuts at: 5971 96 Size: 138 Fragments arranged by size: 1712 1443 1152 1130 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6013 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 2333 4142 2333 Size: 1809 4204 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI