(Circular) (Six-base) MAPSORT of: pFN21AB7731.seq Check: 4782 from: 1 to: 6001 pFN21AB7731 6001 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3811 278 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3344 1937 Size: 1407 4594 Acc65I G'GTAC_C Cuts at: 3322 3322 Size: 6001 AclI AA'CG_TT Cuts at: 1563 4128 4501 1563 Size: 2565 373 3063 Fragments arranged by size: 3063 2565 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4064 5448 5716 1632 Size: 2432 1384 268 1917 Fragments arranged by size: 2432 1917 1384 268 AfeI AGC'GCT Cuts at: 2987 2987 Size: 6001 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5804 AflIII A'CryG_T Cuts at: 1203 4900 5877 1203 Size: 3697 977 1327 Fragments arranged by size: 3697 1327 977 AgeI A'CCGG_T Cuts at: 4832 4832 Size: 6001 AhdI GACnn_n'nnGTC Cuts at: 4730 4730 Size: 6001 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6001 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2079 2291 2987 1184 Size: 895 212 696 4198 Fragments arranged by size: 4198 895 696 212 AlwNI CAG_nnn'CTG Cuts at: 2032 2201 2967 3744 5316 2032 Size: 169 766 777 1572 2717 Fragments arranged by size: 2717 1572 777 766 169 ApaI G_GGCC'C Cuts at: 2445 2445 Size: 6001 ApaLI G'TGCA_C Cuts at: 2047 4058 5214 2047 Size: 2011 1156 2834 Fragments arranged by size: 2834 2011 1156 ApoI r'AATT_y Cuts at: 1493 2639 3310 3536 3688 5797 1493 Size: 1146 671 226 152 2109 1697 Fragments arranged by size: 2109 1697 1146 671 226 152 AseI AT'TA_AT Cuts at: 160 1032 4555 160 Size: 872 3523 1606 Fragments arranged by size: 3523 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 AvaI C'yCGr_G Cuts at: 1870 1943 3326 1870 Size: 73 1383 4545 Fragments arranged by size: 4545 1383 73 BamHI G'GATC_C Cuts at: 3331 3698 3331 Size: 367 5634 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2828 2953 3103 3322 4777 Size: 325 217 627 1041 125 150 219 1455 Cuts at: 4777 618 Size: 1842 Fragments arranged by size: 1842 1455 1041 627 325 219 217 150 125 BanII G_rGCy'C Cuts at: 1391 2222 2239 2445 2570 3012 3320 1391 Size: 831 17 206 125 442 308 4072 Fragments arranged by size: 4072 831 442 308 206 125 17 BbeI G_GCGC'C Cuts at: 2832 2957 3107 2832 Size: 125 150 5726 Fragments arranged by size: 5726 150 125 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3115 928 Size: 971 1216 3814 Fragments arranged by size: 3814 1216 971 BbvCI CC'TCA_GC Cuts at: 2461 2461 Size: 6001 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3073 3107 4192 4226 1903 Size: 34 1136 34 1085 34 3678 Fragments arranged by size: 3678 1136 1085 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3893 5103 3893 Size: 1210 4791 BclI T'GATC_A Cuts at: 1548 1548 Size: 6001 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2574 2951 4612 Size: 107 122 71 631 605 902 377 1661 Cuts at: 4612 136 Size: 1525 Fragments arranged by size: 1661 1525 902 631 605 377 122 107 71 BglII A'GATC_T Cuts at: 2481 2721 3238 5996 2481 Size: 240 517 2758 2486 Fragments arranged by size: 2758 2486 517 240 BlpI GC'TnA_GC Cuts at: 3418 3418 Size: 6001 Bme1580I G_kGCm'C Cuts at: 1790 2051 2288 2445 2874 4062 5218 1790 Size: 261 237 157 429 1188 1156 2573 Fragments arranged by size: 2573 1188 1156 429 261 237 157 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6001 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2298 3732 4690 Size: 416 53 83 399 24 881 1434 958 Cuts at: 4690 442 Size: 1753 Fragments arranged by size: 1753 1434 958 881 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2796 1056 Size: 1740 4261 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2276 2276 Size: 6001 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2054 2427 4661 1239 Size: 815 373 2234 2579 Fragments arranged by size: 2579 2234 815 373 Bpu10I CC'TnA_GC Cuts at: 2461 2805 2461 Size: 344 5657 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4126 4991 5289 4126 Size: 865 298 4838 Fragments arranged by size: 4838 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4664 882 Size: 617 3165 2219 Fragments arranged by size: 3165 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2842 3076 3792 493 Size: 711 1638 234 716 2702 Fragments arranged by size: 2702 1638 716 711 234 BsaBI GATnn'nnATC Cuts at: 2720 5995 2720 Size: 3275 2726 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2829 2954 3104 3808 Size: 53 83 186 936 1296 125 150 704 Cuts at: 3808 4190 4825 275 Size: 382 635 1451 Fragments arranged by size: 1451 1296 936 704 635 382 186 150 125 83 53 BsaWI w'CCGG_w Cuts at: 4434 4832 5106 5253 5664 4434 Size: 398 274 147 411 4771 Fragments arranged by size: 4771 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2400 2430 1178 Size: 30 165 30 997 30 4749 Fragments arranged by size: 4749 997 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2355 2449 1186 Size: 1169 94 4738 Fragments arranged by size: 4738 1169 94 BseYI C'CCAG_C Cuts at: 2239 2578 5204 5700 2239 Size: 339 2626 496 2540 Fragments arranged by size: 2626 2540 496 339 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2765 1163 Size: 573 1029 4399 Fragments arranged by size: 4399 1029 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2282 2669 3451 4212 5240 1540 Size: 275 186 281 387 782 761 1028 2301 Fragments arranged by size: 2301 1028 782 761 387 281 275 186 BsiHKAI G_wGCw'C Cuts at: 2051 2222 4062 4147 5218 2051 Size: 171 1840 85 1071 2834 Fragments arranged by size: 2834 1840 1071 171 85 BsmI GAATG_Cn' Cuts at: 3512 3605 3512 Size: 93 5908 BsmBI CGTCTCn'nnnn_ Cuts at: 3264 3264 Size: 6001 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2222 2239 2288 2445 2570 2874 Size: 399 261 171 17 49 157 125 304 Cuts at: 2874 3012 3320 4062 4147 5218 1391 Size: 138 308 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 304 261 171 157 138 125 85 49 17 BspEI T'CCGG_A Cuts at: 5664 5664 Size: 6001 BspHI T'CATG_A Cuts at: 3890 5620 3890 Size: 1730 4271 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2700 3063 3356 844 Size: 1856 363 293 3489 Fragments arranged by size: 3489 1856 363 293 BsrBI CCG'CTC Cuts at: 1394 1823 2821 3888 1394 Size: 429 998 1067 3507 Fragments arranged by size: 3507 1067 998 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4496 4670 65 Size: 1183 3248 174 1396 Fragments arranged by size: 3248 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2664 2736 2788 4645 4832 1700 Size: 110 854 72 52 1857 187 2869 Fragments arranged by size: 2869 1857 854 187 110 72 52 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6001 BssHII G'CGCG_C Cuts at: 1925 2613 1925 Size: 688 5313 BssSI C'ACGA_G Cuts at: 2608 4061 5073 2608 Size: 1453 1012 3536 Fragments arranged by size: 3536 1453 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6001 BstBI TT'CG_AA Cuts at: 4805 4805 Size: 6001 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6001 BstXI CCAn_nnnn'nTGG Cuts at: 2179 2327 3206 2179 Size: 148 879 4974 Fragments arranged by size: 4974 879 148 BstYI r'GATC_y Cuts at: 1972 2481 2721 3238 3331 3698 4083 4100 5552 Size: 509 240 517 93 367 385 17 1452 Cuts at: 5552 5638 5650 5996 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 517 509 385 367 346 240 93 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 3018 3099 3205 513 Size: 552 1953 81 106 3309 Fragments arranged by size: 3309 1953 552 106 81 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5262 Fragments arranged by size: 5262 683 56 BtsI GCAGTG_nn' Cuts at: 2538 3517 4311 4331 2538 Size: 979 794 20 4208 Fragments arranged by size: 4208 979 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6001 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5966 DraI TTT'AAA Cuts at: 3305 3305 Size: 6001 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5008 809 Size: 507 3692 1802 Fragments arranged by size: 3692 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2279 2666 2685 Size: 54 1425 50 275 18 449 387 19 Cuts at: 2685 2836 2854 2940 3156 3448 4337 5966 8 Size: 151 18 86 216 292 889 1629 43 Fragments arranged by size: 1629 1425 889 449 387 292 275 216 151 86 54 50 43 19 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2279 2666 3448 1537 Size: 275 467 387 782 4090 Fragments arranged by size: 4090 782 467 387 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3931 1353 Size: 2578 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4588 4962 5108 387 Size: 1064 138 2999 374 146 1280 Fragments arranged by size: 2999 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2220 2220 Size: 6001 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2054 2427 4064 4661 5448 5716 1239 Size: 393 422 373 1637 597 787 268 1524 Fragments arranged by size: 1637 1524 787 597 422 393 373 268 EcoO109I rG'GnC_Cy Cuts at: 2029 2029 Size: 6001 EcoRI G'AATT_C Cuts at: 2639 3310 3688 2639 Size: 671 378 4952 Fragments arranged by size: 4952 671 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 6001 FspI TGC'GCA Cuts at: 4507 4507 Size: 6001 HaeII r_GCGC'y Cuts at: 1678 2832 2957 2989 2999 3051 3107 5148 1678 Size: 1154 125 32 10 52 56 2041 2531 Fragments arranged by size: 2531 2041 1154 125 56 52 32 10 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2879 2911 4642 4674 4716 Size: 32 135 32 1307 32 1731 32 42 Cuts at: 4716 4748 1373 Size: 32 2626 Fragments arranged by size: 2626 1731 1307 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3345 3591 669 Size: 988 281 1407 246 3079 Fragments arranged by size: 3079 1407 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6001 HpaI GTT'AAC Cuts at: 3591 3591 Size: 6001 KasI G'GCGC_C Cuts at: 2828 2953 3103 2828 Size: 125 150 5726 Fragments arranged by size: 5726 150 125 KpnI G_GTAC'C Cuts at: 3326 3326 Size: 6001 MfeI C'AATT_G Cuts at: 3600 3600 Size: 6001 MluI A'CGCG_T Cuts at: 5877 5877 Size: 6001 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5115 5299 1305 Size: 3810 184 2007 Fragments arranged by size: 3810 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2687 2838 10 Size: 54 1425 343 855 151 3173 Fragments arranged by size: 3173 1425 855 343 151 54 MslI CAynn'nnrTG Cuts at: 518 1166 3204 3959 4318 4477 518 Size: 648 2038 755 359 159 2042 Fragments arranged by size: 2042 2038 755 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2271 2532 2620 2834 2860 2967 3417 4094 Size: 665 261 88 214 26 107 450 677 Cuts at: 4094 5242 5487 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 665 450 261 245 214 107 88 26 NaeI GCC'GGC Cuts at: 2666 2790 2666 Size: 124 5877 NarI GG'CG_CC Cuts at: 2829 2954 3104 2829 Size: 125 150 5726 Fragments arranged by size: 5726 150 125 NcoI C'CATG_G Cuts at: 513 1065 3018 3099 3205 513 Size: 552 1953 81 106 3309 Fragments arranged by size: 3309 1953 552 106 81 NdeI CA'TA_TG Cuts at: 387 387 Size: 6001 NgoMIV G'CCGG_C Cuts at: 2664 2788 2664 Size: 124 5877 NheI G'CTAG_C Cuts at: 1052 2792 1052 Size: 1740 4261 NotI GC'GGCC_GC Cuts at: 3448 3448 Size: 6001 NspI r_CATG'y Cuts at: 1132 1229 2278 3359 4904 1132 Size: 97 1049 1081 1545 2229 Fragments arranged by size: 2229 1545 1081 1049 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6001 PciI A'CATG_T Cuts at: 4900 4900 Size: 6001 PflMI CCAn_nnn'nTGG Cuts at: 1108 3019 1108 Size: 1911 4090 PfoI T'CCnGG_A Cuts at: 2404 2404 Size: 6001 PmeI GTTT'AAAC Cuts at: 3305 3305 Size: 6001 PmlI CAC'GTG Cuts at: 2842 2842 Size: 6001 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4070 4102 5583 5615 4070 Size: 32 1481 32 4456 Fragments arranged by size: 4456 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6001 PsiI TTA'TAA Cuts at: 3571 3571 Size: 6001 PspOMI G'GGCC_C Cuts at: 2441 2441 Size: 6001 PstI C_TGCA'G Cuts at: 830 2545 3060 3353 830 Size: 1715 515 293 3478 Fragments arranged by size: 3478 1715 515 293 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6001 PvuII CAG'CTG Cuts at: 2271 2532 2620 2860 2967 2271 Size: 261 88 240 107 5305 Fragments arranged by size: 5305 261 240 107 88 SacI G_AGCT'C Cuts at: 2222 2222 Size: 6001 SalI G'TCGA_C Cuts at: 1936 3343 1936 Size: 1407 4594 SbfI CC_TGCA'GG Cuts at: 3060 3353 3060 Size: 293 5708 ScaI AGT'ACT Cuts at: 4249 5961 4249 Size: 1712 4289 SfcI C'TryA_G Cuts at: 826 1045 2104 2131 2541 3056 3169 3349 4484 Size: 219 1059 27 410 515 113 180 1135 Cuts at: 4484 5165 5356 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1059 681 515 410 219 191 180 113 27 SfoI GGC'GCC Cuts at: 2830 2955 3105 2830 Size: 125 150 5726 Fragments arranged by size: 5726 150 125 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6001 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6001 SmaI CCC'GGG Cuts at: 1872 3328 1872 Size: 1456 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4105 5006 5268 820 Size: 197 926 2162 901 262 1553 Fragments arranged by size: 2162 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3792 493 Size: 3299 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6001 SphI G_CATG'C Cuts at: 1132 2278 3359 1132 Size: 1146 1081 3774 Fragments arranged by size: 3774 1146 1081 SspI AAT'ATT Cuts at: 5 52 3925 5 Size: 47 3873 2081 Fragments arranged by size: 3873 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6001 StyI C'CwwG_G Cuts at: 513 1065 2769 3018 3099 3205 3440 513 Size: 552 1704 249 81 106 235 3074 Fragments arranged by size: 3074 1704 552 249 235 106 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2410 4028 4045 4198 4383 4828 325 Size: 1255 830 1618 17 153 185 445 1498 Fragments arranged by size: 1618 1498 1255 830 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2748 4247 5959 Size: 274 80 33 51 1443 771 1499 1712 Cuts at: 5959 96 Size: 138 Fragments arranged by size: 1712 1499 1443 771 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2176 2208 2400 2432 5961 5993 2176 Size: 32 192 32 3529 32 2184 Fragments arranged by size: 3529 2184 192 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6001 XbaI T'CTAG_A Cuts at: 3337 3337 Size: 6001 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2306 2306 Size: 6001 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6001 XmaI C'CCGG_G Cuts at: 1870 3326 1870 Size: 1456 4545 XmnI GAAnn'nnTTC Cuts at: 4130 4130 Size: 6001 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3809 276 Size: 53 83 186 936 2275 2468 Fragments arranged by size: 2468 2275 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI NruI NsiI PacI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI