(Circular) (Six-base) MAPSORT of: pFN21AB7726.seq Check: 167 from: 1 to: 6145 pFN21AB7726 6145 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3285 3285 Size: 6145 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2124 3955 278 Size: 53 83 186 936 588 1831 2468 Fragments arranged by size: 2468 1831 936 588 186 83 53 AccI GT'mk_AC Cuts at: 1937 3488 1937 Size: 1551 4594 Acc65I G'GTAC_C Cuts at: 3466 3466 Size: 6145 AclI AA'CG_TT Cuts at: 1563 4272 4645 1563 Size: 2709 373 3063 Fragments arranged by size: 3063 2709 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4208 5592 5860 1632 Size: 2576 1384 268 1917 Fragments arranged by size: 2576 1917 1384 268 AfeI AGC'GCT Cuts at: 2034 2034 Size: 6145 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5948 AflIII A'CryG_T Cuts at: 1203 5044 6021 1203 Size: 3841 977 1327 Fragments arranged by size: 3841 1327 977 AgeI A'CCGG_T Cuts at: 4976 4976 Size: 6145 AhdI GACnn_n'nnGTC Cuts at: 4874 4874 Size: 6145 AleI CACnn'nnGTG Cuts at: 1166 3416 1166 Size: 2250 3895 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2444 1184 Size: 1260 4885 AlwNI CAG_nnn'CTG Cuts at: 2324 3888 5460 2324 Size: 1564 1572 3009 Fragments arranged by size: 3009 1572 1564 ApaI G_GGCC'C Cuts at: 3444 3444 Size: 6145 ApaLI G'TGCA_C Cuts at: 3053 3419 4202 5358 3053 Size: 366 783 1156 3840 Fragments arranged by size: 3840 1156 783 366 ApoI r'AATT_y Cuts at: 1493 2903 3454 3680 3832 5941 1493 Size: 1410 551 226 152 2109 1697 Fragments arranged by size: 2109 1697 1410 551 226 152 AseI AT'TA_AT Cuts at: 160 1032 4699 160 Size: 872 3667 1606 Fragments arranged by size: 3667 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 AvaI C'yCGr_G Cuts at: 1870 1943 2246 3434 3470 1870 Size: 73 303 1188 36 4545 Fragments arranged by size: 4545 1188 303 73 36 BamHI G'GATC_C Cuts at: 2109 3475 3842 2109 Size: 1366 367 4412 Fragments arranged by size: 4412 1366 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3466 4921 618 Size: 325 217 627 1679 1455 1842 Fragments arranged by size: 1842 1679 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2815 2884 3097 3444 3464 1391 Size: 1424 69 213 347 20 4072 Fragments arranged by size: 4072 1424 347 213 69 20 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2985 3003 928 Size: 971 1086 18 4070 Fragments arranged by size: 4070 1086 971 18 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4336 4370 1903 Size: 34 2399 34 3678 Fragments arranged by size: 3678 2399 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4037 5247 4037 Size: 1210 4935 BclI T'GATC_A Cuts at: 1548 1548 Size: 6145 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4756 136 Size: 107 122 71 631 605 3084 1525 Fragments arranged by size: 3084 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6140 6140 Size: 6145 BlpI GC'TnA_GC Cuts at: 3562 3562 Size: 6145 Bme1580I G_kGCm'C Cuts at: 1790 3057 3423 3444 4206 5362 1790 Size: 1267 366 21 762 1156 2573 Fragments arranged by size: 2573 1267 1156 762 366 21 BmgBI CAC'GTC Cuts at: 1318 2074 2233 3171 1318 Size: 756 159 938 4292 Fragments arranged by size: 4292 938 756 159 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2544 2682 3876 Size: 416 53 83 399 24 1127 138 1194 Cuts at: 3876 4834 442 Size: 958 1753 Fragments arranged by size: 1753 1194 1127 958 416 399 138 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6145 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2301 2689 2799 4805 1239 Size: 1062 388 110 2006 2579 Fragments arranged by size: 2579 2006 1062 388 110 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2835 3272 4270 5135 5433 2835 Size: 437 998 865 298 3547 Fragments arranged by size: 3547 998 865 437 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4808 882 Size: 617 3309 2219 Fragments arranged by size: 3309 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2371 3214 3936 493 Size: 711 1167 843 722 2702 Fragments arranged by size: 2702 1167 843 722 711 BsaBI GATnn'nnATC Cuts at: 6139 6139 Size: 6145 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2121 3952 4334 4969 Size: 53 83 186 936 588 1831 382 635 Cuts at: 4969 275 Size: 1451 Fragments arranged by size: 1831 1451 936 635 588 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3311 4578 4976 5250 5397 5808 3311 Size: 1267 398 274 147 411 3648 Fragments arranged by size: 3648 1267 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2211 2241 2455 2485 2687 Size: 30 165 30 808 30 214 30 202 Cuts at: 2687 2717 1178 Size: 30 4606 Fragments arranged by size: 4606 808 214 202 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2056 2825 1186 Size: 870 769 4506 Fragments arranged by size: 4506 870 769 BseYI C'CCAG_C Cuts at: 2035 2077 5348 5844 2035 Size: 42 3271 496 2336 Fragments arranged by size: 3271 2336 496 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2310 2816 1163 Size: 573 574 506 4492 Fragments arranged by size: 4492 574 573 506 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2346 3595 4356 5384 1540 Size: 275 186 345 1249 761 1028 2301 Fragments arranged by size: 2301 1249 1028 761 345 275 186 BsiHKAI G_wGCw'C Cuts at: 2158 2238 2370 2945 3057 3423 4206 4291 5362 Size: 80 132 575 112 366 783 85 1071 Cuts at: 5362 2158 Size: 2941 Fragments arranged by size: 2941 1071 783 575 366 132 112 85 80 BsmI GAATG_Cn' Cuts at: 3656 3749 3656 Size: 93 6052 BsmBI CGTCTCn'nnnn_ Cuts at: 2688 3161 2688 Size: 473 5672 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2158 2238 2370 2815 2884 2945 3057 Size: 399 368 80 132 445 69 61 112 Cuts at: 3057 3097 3423 3444 3464 4206 4291 5362 1391 Size: 40 326 21 20 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 445 399 368 326 132 112 85 80 69 61 40 21 20 BspEI T'CCGG_A Cuts at: 5808 5808 Size: 6145 BspHI T'CATG_A Cuts at: 4034 5764 4034 Size: 1730 4415 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3263 3285 3500 844 Size: 2419 22 215 3489 Fragments arranged by size: 3489 2419 215 22 BsrBI CCG'CTC Cuts at: 1394 1823 4032 1394 Size: 429 2209 3507 Fragments arranged by size: 3507 2209 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4640 4814 65 Size: 1183 3392 174 1396 Fragments arranged by size: 3392 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3006 3105 4789 4976 1700 Size: 110 1196 99 1684 187 2869 Fragments arranged by size: 2869 1684 1196 187 110 99 BsrGI T'GTAC_A Cuts at: 96 2292 3187 96 Size: 2196 895 3054 Fragments arranged by size: 3054 2196 895 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6145 BssSI C'ACGA_G Cuts at: 4205 5217 4205 Size: 1012 5133 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6145 BstBI TT'CG_AA Cuts at: 4949 4949 Size: 6145 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6145 BstYI r'GATC_y Cuts at: 1972 2109 3475 3842 4227 4244 5696 5782 5794 Size: 137 1366 367 385 17 1452 86 12 Cuts at: 5794 6140 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1366 385 367 346 137 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4654 Fragments arranged by size: 4654 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2158 487 Size: 683 56 932 4474 Fragments arranged by size: 4474 932 683 56 BtsI GCAGTG_nn' Cuts at: 2188 2322 2743 3076 3328 3661 4455 4475 2188 Size: 134 421 333 252 333 794 20 3858 Fragments arranged by size: 3858 794 421 333 333 252 134 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6145 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6110 DraI TTT'AAA Cuts at: 3449 3449 Size: 6145 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5152 809 Size: 507 3836 1802 Fragments arranged by size: 3836 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2343 3107 3592 Size: 54 1425 50 275 18 513 764 485 Cuts at: 3592 4481 6110 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 764 513 485 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2343 3592 1537 Size: 275 531 1249 4090 Fragments arranged by size: 4090 1249 531 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4075 1353 Size: 2722 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3260 4732 5106 5252 387 Size: 1064 138 1671 1472 374 146 1280 Fragments arranged by size: 1671 1472 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2301 2689 2799 4208 4805 5592 5860 Size: 393 669 388 110 1409 597 787 268 Cuts at: 5860 1239 Size: 1524 Fragments arranged by size: 1524 1409 787 669 597 393 388 268 110 EcoNI CCTnn'n_nnAGG Cuts at: 2475 2475 Size: 6145 EcoO109I rG'GnC_Cy Cuts at: 2252 2543 2747 3440 2252 Size: 291 204 693 4957 Fragments arranged by size: 4957 693 291 204 EcoRI G'AATT_C Cuts at: 3454 3832 3454 Size: 378 5767 FspI TGC'GCA Cuts at: 4651 4651 Size: 6145 HaeII r_GCGC'y Cuts at: 1678 2036 2494 5292 1678 Size: 358 458 2798 2531 Fragments arranged by size: 2798 2531 458 358 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2222 2254 2687 2719 4786 Size: 32 135 32 650 32 433 32 2067 Cuts at: 4786 4818 4860 4892 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2067 650 433 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2663 3368 3489 3735 669 Size: 988 281 725 705 121 246 3079 Fragments arranged by size: 3079 988 725 705 281 246 121 HindIII A'AGCT_T Cuts at: 748 748 Size: 6145 HpaI GTT'AAC Cuts at: 3735 3735 Size: 6145 KpnI G_GTAC'C Cuts at: 3470 3470 Size: 6145 MfeI C'AATT_G Cuts at: 3744 3744 Size: 6145 MluI A'CGCG_T Cuts at: 6021 6021 Size: 6145 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2804 5259 5443 1305 Size: 789 710 2455 184 2007 Fragments arranged by size: 2455 2007 789 710 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4323 Fragments arranged by size: 4323 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3416 4103 4462 4621 518 Size: 648 2250 687 359 159 2042 Fragments arranged by size: 2250 2042 687 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2913 3001 3561 4238 5386 5631 1606 Size: 1307 88 560 677 1148 245 2120 Fragments arranged by size: 2120 1307 1148 677 560 245 88 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4654 Fragments arranged by size: 4654 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6145 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6145 NotI GC'GGCC_GC Cuts at: 2343 3592 2343 Size: 1249 4896 NspI r_CATG'y Cuts at: 1132 1229 2385 3503 5048 1132 Size: 97 1156 1118 1545 2229 Fragments arranged by size: 2229 1545 1156 1118 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6145 PciI A'CATG_T Cuts at: 5044 5044 Size: 6145 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6145 PmeI GTTT'AAAC Cuts at: 3449 3449 Size: 6145 PmlI CAC'GTG Cuts at: 2371 3214 2371 Size: 843 5302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4214 4246 5727 5759 4214 Size: 32 1481 32 4600 Fragments arranged by size: 4600 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2252 2747 2252 Size: 495 5650 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6145 PsiI TTA'TAA Cuts at: 3715 3715 Size: 6145 PspOMI G'GGCC_C Cuts at: 3440 3440 Size: 6145 PstI C_TGCA'G Cuts at: 830 2183 2537 3282 3497 830 Size: 1353 354 745 215 3478 Fragments arranged by size: 3478 1353 745 354 215 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6145 PvuII CAG'CTG Cuts at: 2913 3001 2913 Size: 88 6057 SalI G'TCGA_C Cuts at: 1936 3487 1936 Size: 1551 4594 SanDI GG'GwC_CC Cuts at: 2252 2252 Size: 6145 SbfI CC_TGCA'GG Cuts at: 3497 3497 Size: 6145 ScaI AGT'ACT Cuts at: 4393 6105 4393 Size: 1712 4433 SexAI A'CCwGG_T Cuts at: 2476 2476 Size: 6145 SfcI C'TryA_G Cuts at: 826 1045 2179 2533 3278 3493 4628 5309 5500 Size: 219 1134 354 745 215 1135 681 191 Cuts at: 5500 826 Size: 1471 Fragments arranged by size: 1471 1135 1134 745 681 354 219 215 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6145 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6145 SmaI CCC'GGG Cuts at: 1872 2248 3472 1872 Size: 376 1224 4545 Fragments arranged by size: 4545 1224 376 SmlI C'TyrA_G Cuts at: 820 1017 1943 2850 3287 4249 5150 5412 820 Size: 197 926 907 437 962 901 262 1553 Fragments arranged by size: 1553 962 926 907 901 437 262 197 SnaBI TAC'GTA Cuts at: 493 3936 493 Size: 3443 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6145 SphI G_CATG'C Cuts at: 1132 2385 3503 1132 Size: 1253 1118 3774 Fragments arranged by size: 3774 1253 1118 SspI AAT'ATT Cuts at: 5 52 4069 5 Size: 47 4017 2081 Fragments arranged by size: 4017 2081 47 StuI AGG'CCT Cuts at: 1517 2576 2621 2702 1517 Size: 1059 45 81 4960 Fragments arranged by size: 4960 1059 81 45 StyI C'CwwG_G Cuts at: 513 1065 2004 2571 2961 3584 513 Size: 552 939 567 390 623 3074 Fragments arranged by size: 3074 939 623 567 552 390 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2520 4172 4189 4342 4527 4972 325 Size: 1255 940 1652 17 153 185 445 1498 Fragments arranged by size: 1652 1498 1255 940 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2292 2592 2598 Size: 274 80 33 51 1443 315 300 6 Cuts at: 2598 3187 4391 6103 96 Size: 589 1204 1712 138 Fragments arranged by size: 1712 1443 1204 589 315 300 274 138 80 51 33 6 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2211 2243 2319 2351 6105 6137 2211 Size: 32 76 32 3754 32 2219 Fragments arranged by size: 3754 2219 76 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6145 XbaI T'CTAG_A Cuts at: 3481 3481 Size: 6145 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6145 XmaI C'CCGG_G Cuts at: 1870 2246 3470 1870 Size: 376 1224 4545 Fragments arranged by size: 4545 1224 376 XmnI GAAnn'nnTTC Cuts at: 4274 4274 Size: 6145 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2122 3953 276 Size: 53 83 186 936 588 1831 2468 Fragments arranged by size: 2468 1831 936 588 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI