(Circular) (Six-base) MAPSORT of: pFN21AB7715.seq Check: 1997 from: 1 to: 5983 pFN21AB7715 5983 bp With 182 enzymes: SgfI * October 20, 2009 10:54 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2678 2788 2678 Size: 110 5873 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3793 278 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3326 1937 Size: 1389 4594 Acc65I G'GTAC_C Cuts at: 3304 3304 Size: 5983 AclI AA'CG_TT Cuts at: 1563 4110 4483 1563 Size: 2547 373 3063 Fragments arranged by size: 3063 2547 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4046 5430 5698 1632 Size: 2414 1384 268 1917 Fragments arranged by size: 2414 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5786 AflIII A'CryG_T Cuts at: 1203 4882 5859 1203 Size: 3679 977 1327 Fragments arranged by size: 3679 1327 977 AgeI A'CCGG_T Cuts at: 2295 4814 2295 Size: 2519 3464 AhdI GACnn_n'nnGTC Cuts at: 4712 4712 Size: 5983 AleI CACnn'nnGTG Cuts at: 1166 2558 1166 Size: 1392 4591 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2576 3059 1184 Size: 1392 483 4108 Fragments arranged by size: 4108 1392 483 AlwNI CAG_nnn'CTG Cuts at: 2044 3726 5298 2044 Size: 1682 1572 2729 Fragments arranged by size: 2729 1682 1572 ApaI G_GGCC'C Cuts at: 2736 2736 Size: 5983 ApaLI G'TGCA_C Cuts at: 4040 5196 4040 Size: 1156 4827 ApoI r'AATT_y Cuts at: 1493 3292 3518 3670 5779 1493 Size: 1799 226 152 2109 1697 Fragments arranged by size: 2109 1799 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4537 160 Size: 872 3505 1606 Fragments arranged by size: 3505 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 AvaI C'yCGr_G Cuts at: 1870 1943 2675 2735 3185 3308 1870 Size: 73 732 60 450 123 4545 Fragments arranged by size: 4545 732 450 123 73 60 BamHI G'GATC_C Cuts at: 2115 3313 3680 2115 Size: 1198 367 4418 Fragments arranged by size: 4418 1198 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2717 2820 3304 4759 618 Size: 325 217 627 930 103 484 1455 1842 Fragments arranged by size: 1842 1455 930 627 484 325 217 103 BanII G_rGCy'C Cuts at: 1391 2273 2492 2736 3302 1391 Size: 882 219 244 566 4072 Fragments arranged by size: 4072 882 566 244 219 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2959 3202 928 Size: 971 347 713 243 3709 Fragments arranged by size: 3709 971 713 347 243 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4174 4208 1903 Size: 34 2237 34 3678 Fragments arranged by size: 3678 2237 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2082 3875 5085 2082 Size: 1793 1210 2980 Fragments arranged by size: 2980 1793 1210 BclI T'GATC_A Cuts at: 1548 2442 2478 2622 1548 Size: 894 36 144 4909 Fragments arranged by size: 4909 894 144 36 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2679 2859 4594 Size: 107 122 71 631 605 1007 180 1735 Cuts at: 4594 136 Size: 1525 Fragments arranged by size: 1735 1525 1007 631 605 180 122 107 71 BglII A'GATC_T Cuts at: 5978 5978 Size: 5983 BlpI GC'TnA_GC Cuts at: 3400 3400 Size: 5983 Bme1580I G_kGCm'C Cuts at: 1790 2736 2823 4044 5200 1790 Size: 946 87 1221 1156 2573 Fragments arranged by size: 2573 1221 1156 946 87 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5983 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3714 4672 442 Size: 416 53 83 399 24 2297 958 1753 Fragments arranged by size: 2297 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5983 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2765 2846 2963 4643 1239 Size: 1526 81 117 1680 2579 Fragments arranged by size: 2579 1680 1526 117 81 Bpu10I CC'TnA_GC Cuts at: 2696 2696 Size: 5983 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4108 4973 5271 4108 Size: 865 298 4820 Fragments arranged by size: 4820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4646 882 Size: 617 3147 2219 Fragments arranged by size: 3147 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3774 493 Size: 711 2570 2702 Fragments arranged by size: 2702 2570 711 BsaBI GATnn'nnATC Cuts at: 2148 2621 5977 2148 Size: 473 3356 2154 Fragments arranged by size: 3356 2154 473 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2772 3790 4172 4807 Size: 53 83 186 936 1239 1018 382 635 Cuts at: 4807 275 Size: 1451 Fragments arranged by size: 1451 1239 1018 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2013 2112 2295 3067 3213 4416 4814 5088 5235 Size: 99 183 772 146 1203 398 274 147 Cuts at: 5235 5646 2013 Size: 411 2350 Fragments arranged by size: 2350 1203 772 411 398 274 183 147 146 99 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5758 Fragments arranged by size: 5758 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2111 3203 1186 Size: 925 1092 3966 Fragments arranged by size: 3966 1092 925 BseYI C'CCAG_C Cuts at: 2549 2780 3146 5186 5682 2549 Size: 231 366 2040 496 2850 Fragments arranged by size: 2850 2040 496 366 231 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2828 3125 1163 Size: 573 1092 297 4021 Fragments arranged by size: 4021 1092 573 297 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2422 2769 3433 4194 5222 1540 Size: 275 186 421 347 664 761 1028 2301 Fragments arranged by size: 2301 1028 761 664 421 347 275 186 BsiHKAI G_wGCw'C Cuts at: 4044 4129 5200 4044 Size: 85 1071 4827 Fragments arranged by size: 4827 1071 85 BsmI GAATG_Cn' Cuts at: 2606 3494 3587 2606 Size: 888 93 5002 Fragments arranged by size: 5002 888 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2273 2492 2736 2823 3302 4044 4129 Size: 399 483 219 244 87 479 742 85 Cuts at: 4129 5200 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 483 479 399 244 219 87 85 BspEI T'CCGG_A Cuts at: 2112 3067 3213 5646 2112 Size: 955 146 2433 2449 Fragments arranged by size: 2449 2433 955 146 BspHI T'CATG_A Cuts at: 3872 5602 3872 Size: 1730 4253 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2678 2788 3338 844 Size: 1834 110 550 3489 Fragments arranged by size: 3489 1834 550 110 BsrBI CCG'CTC Cuts at: 1394 1823 3870 1394 Size: 429 2047 3507 Fragments arranged by size: 3507 2047 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4478 4652 65 Size: 1183 3230 174 1396 Fragments arranged by size: 3230 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2295 2764 2853 3021 3164 4627 4814 Size: 110 485 469 89 168 143 1463 187 Cuts at: 4814 1700 Size: 2869 Fragments arranged by size: 2869 1463 485 469 187 168 143 110 89 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5983 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5983 BssSI C'ACGA_G Cuts at: 3101 4043 5055 3101 Size: 942 1012 4029 Fragments arranged by size: 4029 1012 942 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5983 BstBI TT'CG_AA Cuts at: 4787 4787 Size: 5983 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5983 BstXI CCAn_nnnn'nTGG Cuts at: 2235 2535 2941 2235 Size: 300 406 5277 Fragments arranged by size: 5277 406 300 BstYI r'GATC_y Cuts at: 1972 2115 3313 3680 4065 4082 5534 5620 5632 Size: 143 1198 367 385 17 1452 86 12 Cuts at: 5632 5978 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1198 385 367 346 143 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2346 2346 Size: 5983 BtgI C'CryG_G Cuts at: 513 1065 2913 513 Size: 552 1848 3583 Fragments arranged by size: 3583 1848 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5244 Fragments arranged by size: 5244 683 56 BtsI GCAGTG_nn' Cuts at: 3499 4293 4313 3499 Size: 794 20 5169 Fragments arranged by size: 5169 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5983 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5948 DraI TTT'AAA Cuts at: 2194 2206 3287 2194 Size: 12 1081 4890 Fragments arranged by size: 4890 1081 12 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2296 4990 809 Size: 507 980 2694 1802 Fragments arranged by size: 2694 1802 980 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2766 3430 4319 Size: 54 1425 50 275 18 936 664 889 Cuts at: 4319 5948 8 Size: 1629 43 Fragments arranged by size: 1629 1425 936 889 664 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2766 3430 1537 Size: 275 954 664 4090 Fragments arranged by size: 4090 954 664 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2096 2346 3054 3135 3146 3913 1353 Size: 743 250 708 81 11 767 3423 Fragments arranged by size: 3423 767 743 708 250 81 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4570 4944 5090 387 Size: 1064 138 2981 374 146 1280 Fragments arranged by size: 2981 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2765 2846 2963 4046 4643 5430 5698 Size: 393 1133 81 117 1083 597 787 268 Cuts at: 5698 1239 Size: 1524 Fragments arranged by size: 1524 1133 1083 787 597 393 268 117 81 EcoO109I rG'GnC_Cy Cuts at: 2740 3181 2740 Size: 441 5542 EcoRI G'AATT_C Cuts at: 3292 3670 3292 Size: 378 5605 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2508 2508 Size: 5983 FspI TGC'GCA Cuts at: 4489 4489 Size: 5983 HaeII r_GCGC'y Cuts at: 1678 5130 1678 Size: 3452 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4624 4656 4698 4730 1373 Size: 32 135 32 3052 32 42 32 2626 Fragments arranged by size: 3052 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3327 3573 669 Size: 988 281 1389 246 3079 Fragments arranged by size: 3079 1389 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5983 HpaI GTT'AAC Cuts at: 3573 3573 Size: 5983 KpnI G_GTAC'C Cuts at: 3308 3308 Size: 5983 MfeI C'AATT_G Cuts at: 3582 3582 Size: 5983 MluI A'CGCG_T Cuts at: 5859 5859 Size: 5983 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2492 5097 5281 1305 Size: 1187 2605 184 2007 Fragments arranged by size: 2605 2007 1187 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4161 Fragments arranged by size: 4161 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2474 2558 3101 3941 4300 4459 518 Size: 648 1308 84 543 840 359 159 2042 Fragments arranged by size: 2042 1308 840 648 543 359 159 84 MspA1I CmG'CkG Cuts at: 1606 2566 2904 3118 3399 4076 5224 5469 1606 Size: 960 338 214 281 677 1148 245 2120 Fragments arranged by size: 2120 1148 960 677 338 281 245 214 NaeI GCC'GGC Cuts at: 2766 2855 3023 3166 2766 Size: 89 168 143 5583 Fragments arranged by size: 5583 168 143 89 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5431 NdeI CA'TA_TG Cuts at: 387 387 Size: 5983 NgoMIV G'CCGG_C Cuts at: 2764 2853 3021 3164 2764 Size: 89 168 143 5583 Fragments arranged by size: 5583 168 143 89 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5983 NotI GC'GGCC_GC Cuts at: 3430 3430 Size: 5983 NspI r_CATG'y Cuts at: 1132 1229 2071 2110 3341 4886 1132 Size: 97 842 39 1231 1545 2229 Fragments arranged by size: 2229 1545 1231 842 97 39 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5983 PciI A'CATG_T Cuts at: 4882 4882 Size: 5983 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5983 PfoI T'CCnGG_A Cuts at: 2074 2074 Size: 5983 PmeI GTTT'AAAC Cuts at: 3287 3287 Size: 5983 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4052 4084 5565 5597 4052 Size: 32 1481 32 4438 Fragments arranged by size: 4438 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5983 PsiI TTA'TAA Cuts at: 2148 3553 2148 Size: 1405 4578 PspOMI G'GGCC_C Cuts at: 2732 2732 Size: 5983 PstI C_TGCA'G Cuts at: 830 2903 3000 3335 830 Size: 2073 97 335 3478 Fragments arranged by size: 3478 2073 335 97 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5983 PvuII CAG'CTG Cuts at: 3118 3118 Size: 5983 RsrII CG'GwC_CG Cuts at: 2293 2293 Size: 5983 SalI G'TCGA_C Cuts at: 1936 3325 1936 Size: 1389 4594 SapI GCTCTTCn'nnn_ Cuts at: 2096 2346 3146 2096 Size: 250 800 4933 Fragments arranged by size: 4933 800 250 SbfI CC_TGCA'GG Cuts at: 3335 3335 Size: 5983 ScaI AGT'ACT Cuts at: 4231 5943 4231 Size: 1712 4271 SexAI A'CCwGG_T Cuts at: 3204 3204 Size: 5983 SfcI C'TryA_G Cuts at: 826 1045 2899 2996 3331 4466 5147 5338 826 Size: 219 1854 97 335 1135 681 191 1471 Fragments arranged by size: 1854 1471 1135 681 335 219 191 97 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5983 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5983 SmaI CCC'GGG Cuts at: 1872 3310 1872 Size: 1438 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4087 4988 5250 820 Size: 197 926 2144 901 262 1553 Fragments arranged by size: 2144 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3774 493 Size: 3281 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5983 SphI G_CATG'C Cuts at: 1132 3341 1132 Size: 2209 3774 SspI AAT'ATT Cuts at: 5 52 3907 5 Size: 47 3855 2081 Fragments arranged by size: 3855 2081 47 StuI AGG'CCT Cuts at: 1517 2180 2507 1517 Size: 663 327 4993 Fragments arranged by size: 4993 663 327 StyI C'CwwG_G Cuts at: 513 1065 2120 3422 513 Size: 552 1055 1302 3074 Fragments arranged by size: 3074 1302 1055 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4010 4027 4180 4365 4810 325 Size: 1255 2430 17 153 185 445 1498 Fragments arranged by size: 2430 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4229 5941 96 Size: 274 80 33 51 1443 2252 1712 138 Fragments arranged by size: 2252 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3142 3174 5943 5975 3142 Size: 32 2769 32 3150 Fragments arranged by size: 3150 2769 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3206 1316 Size: 1890 4093 XbaI T'CTAG_A Cuts at: 3319 3319 Size: 5983 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2082 2082 Size: 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5983 XmaI C'CCGG_G Cuts at: 1870 3308 1870 Size: 1438 4545 XmnI GAAnn'nnTTC Cuts at: 2813 3063 4112 2813 Size: 250 1049 4684 Fragments arranged by size: 4684 1049 250 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3791 276 Size: 53 83 186 936 2257 2468 Fragments arranged by size: 2468 2257 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspXI PsrI SacI SacII SanDI SfiI SfoI SrfI SwaI