Restriction map for
pFN21AB7711
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# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AocI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaBI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eam1105I EarI
Eco81I EcoO65I EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HinfI HpaI KpnI Ksp632I MaeI
MaeIII MamI MfeI MluI MroI
MseI MunI NcoI NdeI NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SapI ScaI SfuI SgrAI SmlI
SnaBI SpeI SphI SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 31 enzymes) #
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AfaI 600 GTAC GT^AC
AflIII 1020 ACACGT A^C[GA][CT]GT
Aor51HI 697 AGCGCT AGC^GCT
BbeI 207 GGCGCC GGCGC^C
BcgI 1059 CGACTGCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 993 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 993 GCTGAGC GC^T[ACGT]AGC
BsaI 1120 GAGACC GGTCTC(1/5)
BsgI 791 GTGCAG GTGCAG(16/14)
BspMI 1229 ACCTGC ACCTGC(4/8)
BssHII 296 GCGCGC G^CGCGC
BstXI 836 CCAAGCGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 483 AGATCC [GA]^GATC[CT]
CelII 993 GCTGAGC GC^T[ACGT]AGC
DraIII 187 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 697 AGCGCT AGC^GCT
EcoNI 1194 CCTCTTTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 207 GGCGCC G^GCGCC
KspI 856 CCGCGG CCGC^GG
MboII 941 TCTTC GAAGA(8/7)
MflI 483 AGATCC [GA]^GATC[CT]
NarI 207 GGCGCC GG^CGCC
PvuII 745 CAGCTG CAG^CTG
RsaI 600 GTAC GT^AC
SacII 856 CCGCGG CCGC^GG
SbfI 1230 CCTGCAGG CCTGCA^GG
SexAI 188 ACCAGGT A^CC[AT]GGT
SfaNI 1210 GATGC GCATC(5/9)
SfoI 207 GGCGCC GGC^GCC
Sse8387I 1230 CCTGCAGG CCTGCA^GG
XhoII 483 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 121 GAATTC [GA]^AATT[CT]
498 GAATTC
Alw44I 282 GTGCAC G^TGCAC
974 GTGCAC
AlwI 484 GATCC GGATC(4/5)
449 GGATC
ApaLI 282 GTGCAC G^TGCAC
974 GTGCAC
ApoI 121 GAATTC [GA]^AATT[CT]
498 GAATTC
AspHI 282 GTGCAC G[AT]GC[AT]^C
974 GTGCAC
BbrPI 355 CACGTG CAC^GTG
1021 CACGTG
BsaAI 355 CACGTG [CT]AC^GT[GA]
1021 CACGTG
BsiEI 227 CGGCCG CG[GA][CT]^CG
1047 CGGCCG
BsiHKAI 282 GTGCAC G[AT]GC[AT]^C
974 GTGCAC
BsmAI 1120 GAGAC GTCTC(1/5)
519 GTCTC
BsrFI 36 GCCGGC [GA]^CCGG[CT]
949 GCCGGC
Cfr10I 36 GCCGGC [GA]^CCGG[CT]
949 GCCGGC
EagI 227 CGGCCG C^GGCCG
1047 CGGCCG
EclXI 227 CGGCCG C^GGCCG
1047 CGGCCG
Eco52I 227 CGGCCG C^GGCCG
1047 CGGCCG
EcoRI 121 GAATTC G^AATTC
498 GAATTC
EcoT14I 140 CCAAGG C^C[AT][AT]GG
848 CCAAGG
HgaI 79 GACGC GACGC(5/10)
946 GACGC
NaeI 36 GCCGGC GCC^GGC
949 GCCGGC
NgoMIV 36 GCCGGC G^CCGGC
949 GCCGGC
NlaIII 393 CATG CATG^
399 CATG
PmaCI 355 CACGTG CAC^GTG
1021 CACGTG
PmlI 355 CACGTG CAC^GTG
1021 CACGTG
PpuMI 214 GGGTCCC [GA]G^G[AT]CC[CT]
851 AGGTCCC
SfiI 396 GGCCATGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
405 GGCCAGCAAGGCC
StyI 140 CCAAGG C^C[AT][AT]GG
848 CCAAGG
Tsp509I 122 AATT ^AATT
499 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AluI AlwNI
AvaI AvaII BalI BanI BanII
BbvI BcnI BglI BmyI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HpaII
HphI ItaI MaeII MboI MluNI
MnlI MscI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV PstI Sau3AI Sau96I ScrFI
SfcI SmaI TaqI TseI TspRI
TthHB8I XmaCI XmaI