Restriction map for
pFN21AB7711
(Circular) (Six-base) MAPSORT of: pFN21AB7711.seq Check: 9454 from: 1 to: 5968
pFN21AB7711 5968 bp
With 182 enzymes: SgfI *
October 20, 2009 10:54 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3778 278
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3311 1937
Size: 1374 4594
Acc65I G'GTAC_C
Cuts at: 3289 3289
Size: 5968
AclI AA'CG_TT
Cuts at: 1563 4095 4468 1563
Size: 2532 373 3063
Fragments arranged by size:
3063 2532 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4031 5415 5683 1632
Size: 2399 1384 268 1917
Fragments arranged by size:
2399 1917 1384 268
AfeI AGC'GCT
Cuts at: 2704 2704
Size: 5968
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5771
AflIII A'CryG_T
Cuts at: 1203 3025 4867 5844 1203
Size: 1822 1842 977 1327
Fragments arranged by size:
1842 1822 1327 977
AgeI A'CCGG_T
Cuts at: 4799 4799
Size: 5968
AhdI GACnn_n'nnGTC
Cuts at: 4697 4697
Size: 5968
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5968
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2420 2897 1184
Size: 1236 477 4255
Fragments arranged by size:
4255 1236 477
AlwNI CAG_nnn'CTG
Cuts at: 2119 2752 2755 2836 3180 3711 5283 2119
Size: 633 3 81 344 531 1572 2804
Fragments arranged by size:
2804 1572 633 531 344 81 3
ApaLI G'TGCA_C
Cuts at: 2287 2979 4025 5181 2287
Size: 692 1046 1156 3074
Fragments arranged by size:
3074 1156 1046 692
ApoI r'AATT_y
Cuts at: 1493 2126 2503 3277 3503 3655 5764 1493
Size: 633 377 774 226 152 2109 1697
Fragments arranged by size:
2109 1697 774 633 377 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4522 160
Size: 872 3490 1606
Fragments arranged by size:
3490 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
AvaI C'yCGr_G
Cuts at: 1870 1943 2029 2164 2216 2308 2553 2712 3293
Size: 73 86 135 52 92 245 159 581
Cuts at: 3293 1870
Size: 4545
Fragments arranged by size:
4545 581 245 159 135 92 86 73
52
BamHI G'GATC_C
Cuts at: 3298 3665 3298
Size: 367 5601
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2171 2197 2212 3289 4744
Size: 325 217 627 384 26 15 1077 1455
Cuts at: 4744 618
Size: 1842
Fragments arranged by size:
1842 1455 1077 627 384 325 217 26
15
BanII G_rGCy'C
Cuts at: 1391 2082 2700 3005 3017 3198 3287 1391
Size: 691 618 305 12 181 89 4072
Fragments arranged by size:
4072 691 618 305 181 89 12
BbeI G_GCGC'C
Cuts at: 2216 2216
Size: 5968
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4997
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3053 3087 4159 4193 1903
Size: 34 1116 34 1072 34 3678
Fragments arranged by size:
3678 1116 1072 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3860 5070 3860
Size: 1210 4758
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5968
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2042 2311 2408
Size: 107 122 71 631 605 370 269 97
Cuts at: 2408 2417 2684 3224 4579 136
Size: 9 267 540 1355 1525
Fragments arranged by size:
1525 1355 631 605 540 370 269 267
122 107 97 71 9
BglII A'GATC_T
Cuts at: 5963 5963
Size: 5968
BlpI GC'TnA_GC
Cuts at: 2999 3385 2999
Size: 386 5582
Bme1580I G_kGCm'C
Cuts at: 1790 2174 2291 2983 4029 5185 1790
Size: 384 117 692 1046 1156 2573
Fragments arranged by size:
2573 1156 1046 692 384 117
BmgBI CAC'GTC
Cuts at: 1318 2280 2292 2338 3034 1318
Size: 962 12 46 696 4252
Fragments arranged by size:
4252 962 696 46 12
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2596 2944 3253
Size: 416 53 83 399 24 1179 348 309
Cuts at: 3253 3699 4657 442
Size: 446 958 1753
Fragments arranged by size:
1753 1179 958 446 416 399 348 309
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5968
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3024 3024
Size: 5968
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2093 3005 3014 4628 1239
Size: 854 912 9 1614 2579
Fragments arranged by size:
2579 1614 912 854 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4093 4958 5256 4093
Size: 865 298 4805
Fragments arranged by size:
4805 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3119 4631 882
Size: 617 1620 1512 2219
Fragments arranged by size:
2219 1620 1512 617
BsaAI yAC'GTr
Cuts at: 493 1204 2362 3028 3759 493
Size: 711 1158 666 731 2702
Fragments arranged by size:
2702 1158 731 711 666
BsaBI GATnn'nnATC
Cuts at: 5962 5962
Size: 5968
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2085 2213 2952 3775
Size: 53 83 186 936 552 128 739 823
Cuts at: 3775 4157 4792 275
Size: 382 635 1451
Fragments arranged by size:
1451 936 823 739 635 552 382 186
128 83 53
BsaWI w'CCGG_w
Cuts at: 4401 4799 5073 5220 5631 4401
Size: 398 274 147 411 4738
Fragments arranged by size:
4738 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5743
Fragments arranged by size:
5743 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2063 2090 2783 2909 1186
Size: 877 27 693 126 4245
Fragments arranged by size:
4245 877 693 126 27
BseYI C'CCAG_C
Cuts at: 2668 2699 2745 2790 2812 2870 2974 3209 5171
Size: 31 46 45 22 58 104 235 1962
Cuts at: 5171 5667 2668
Size: 496 2969
Fragments arranged by size:
2969 1962 496 235 104 58 46 45
31 22
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2817 1163
Size: 573 1081 4314
Fragments arranged by size:
4314 1081 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2235 3055 3418 4179 5207 1540
Size: 275 186 234 820 363 761 1028 2301
Fragments arranged by size:
2301 1028 820 761 363 275 234 186
BsiHKAI G_wGCw'C
Cuts at: 2291 2983 4029 4114 5185 2291
Size: 692 1046 85 1071 3074
Fragments arranged by size:
3074 1071 1046 692 85
BsmI GAATG_Cn'
Cuts at: 3479 3572 3479
Size: 93 5875
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2082 2174 2291 2700 2983 3005 3017
Size: 399 292 92 117 409 283 22 12
Cuts at: 3017 3198 3287 4029 4114 5185 1391
Size: 181 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 409 399 292 283 181
117 92 89 85 22 12
BspEI T'CCGG_A
Cuts at: 5631 5631
Size: 5968
BspHI T'CATG_A
Cuts at: 3857 5587 3857
Size: 1730 4238
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3243 3323 844
Size: 2399 80 3489
Fragments arranged by size:
3489 2399 80
BsrBI CCG'CTC
Cuts at: 1394 1823 2614 2625 3171 3855 1394
Size: 429 791 11 546 684 3507
Fragments arranged by size:
3507 791 684 546 429 11
BsrDI GCAATG_nn'
Cuts at: 65 1248 4463 4637 65
Size: 1183 3215 174 1396
Fragments arranged by size:
3215 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2041 2954 4612 4799 1700
Size: 110 231 913 1658 187 2869
Fragments arranged by size:
2869 1658 913 231 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5968
BssHII G'CGCG_C
Cuts at: 1925 2301 1925
Size: 376 5592
BssSI C'ACGA_G
Cuts at: 4028 5040 4028
Size: 1012 4956
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2119 2755 3180 794
Size: 1325 636 425 3582
Fragments arranged by size:
3582 1325 636 425
BstBI TT'CG_AA
Cuts at: 4772 4772
Size: 5968
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5968
BstXI CCAn_nnnn'nTGG
Cuts at: 2848 2848
Size: 5968
BstYI r'GATC_y
Cuts at: 1972 2488 3298 3665 4050 4067 5519 5605 5617
Size: 516 810 367 385 17 1452 86 12
Cuts at: 5617 5963 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 810 516 385 367 346 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2182 2861 3049 513
Size: 552 939 178 679 188 3432
Fragments arranged by size:
3432 939 679 552 188 178
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5229
Fragments arranged by size:
5229 683 56
BtsI GCAGTG_nn'
Cuts at: 2120 3181 3484 4278 4298 2120
Size: 1061 303 794 20 3790
Fragments arranged by size:
3790 1061 794 303 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5968
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5933
DraI TTT'AAA
Cuts at: 3272 3272
Size: 5968
DraIII CAC_nnn'GTG
Cuts at: 2197 2197
Size: 5968
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4975 809
Size: 507 3659 1802
Fragments arranged by size:
3659 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2232 2400 2439
Size: 54 1425 50 275 18 402 168 39
Cuts at: 2439 2850 3052 3135 3415 4304 5933 8
Size: 411 202 83 280 889 1629 43
Fragments arranged by size:
1629 1425 889 411 402 280 275 202
168 83 54 50 43 39 18
EagI C'GGCC_G
Cuts at: 1537 1812 2232 3052 3415 1537
Size: 275 420 820 363 4090
Fragments arranged by size:
4090 820 420 363 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3898 1353
Size: 2545 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2024 2060 2468 2501 4555 4929
Size: 1064 138 435 36 408 33 2054 374
Cuts at: 4929 5075 387
Size: 146 1280
Fragments arranged by size:
2054 1280 1064 435 408 374 146 138
36 33
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2093 3005 3014 4031 4628 5415 5683
Size: 393 461 912 9 1017 597 787 268
Cuts at: 5683 1239
Size: 1524
Fragments arranged by size:
1524 1017 912 787 597 461 393 268
9
EcoNI CCTnn'n_nnAGG
Cuts at: 3203 3203
Size: 5968
EcoO109I rG'GnC_Cy
Cuts at: 2149 2220 2320 2857 2989 3083 3258 2149
Size: 71 100 537 132 94 175 4859
Fragments arranged by size:
4859 537 175 132 100 94 71
EcoRI G'AATT_C
Cuts at: 2126 2503 3277 3655 2126
Size: 377 774 378 4439
Fragments arranged by size:
4439 774 378 377
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2599 2599
Size: 5968
FspI TGC'GCA
Cuts at: 4474 4474
Size: 5968
HaeII r_GCGC'y
Cuts at: 1678 2216 2631 2706 5115 1678
Size: 538 415 75 2409 2531
Fragments arranged by size:
2531 2409 538 415 75
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4609 4641 4683 4715 1373
Size: 32 135 32 3037 32 42 32 2626
Fragments arranged by size:
3037 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3312 3558 669
Size: 988 281 1374 246 3079
Fragments arranged by size:
3079 1374 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5968
HpaI GTT'AAC
Cuts at: 3558 3558
Size: 5968
KasI G'GCGC_C
Cuts at: 2212 2212
Size: 5968
KpnI G_GTAC'C
Cuts at: 3293 3293
Size: 5968
MfeI C'AATT_G
Cuts at: 3567 3567
Size: 5968
MluI A'CGCG_T
Cuts at: 5844 5844
Size: 5968
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3255 5082 5266 1305
Size: 1950 1827 184 2007
Fragments arranged by size:
2007 1950 1827 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2402 2852 3137 10
Size: 54 1425 343 570 450 285 2841
Fragments arranged by size:
2841 1425 570 450 343 285 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2396 2402 2546 3926 4285 4444 518
Size: 648 1230 6 144 1380 359 159 2042
Fragments arranged by size:
2042 1380 1230 648 359 159 144 6
MspA1I CmG'CkG
Cuts at: 1606 2210 2725 2752 2836 2863 3384 4061 5209
Size: 604 515 27 84 27 521 677 1148
Cuts at: 5209 5454 1606
Size: 245 2120
Fragments arranged by size:
2120 1148 677 604 521 515 245 84
27 27
NaeI GCC'GGC
Cuts at: 2043 2956 2043
Size: 913 5055
NarI GG'CG_CC
Cuts at: 2213 2213
Size: 5968
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4477
Fragments arranged by size:
4477 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5968
NgoMIV G'CCGG_C
Cuts at: 2041 2954 2041
Size: 913 5055
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5968
NotI GC'GGCC_GC
Cuts at: 3415 3415
Size: 5968
NspI r_CATG'y
Cuts at: 1132 1229 3326 4871 1132
Size: 97 2097 1545 2229
Fragments arranged by size:
2229 2097 1545 97
PasI CC'CwG_GG
Cuts at: 1348 3159 1348
Size: 1811 4157
PciI A'CATG_T
Cuts at: 4867 4867
Size: 5968
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5968
PmeI GTTT'AAAC
Cuts at: 3272 3272
Size: 5968
PmlI CAC'GTG
Cuts at: 2362 3028 2362
Size: 666 5302
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4037 4069 5550 5582 4037
Size: 32 1481 32 4423
Fragments arranged by size:
4423 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2220 2857 2220
Size: 637 5331
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5968
PsiI TTA'TAA
Cuts at: 3538 3538
Size: 5968
PstI C_TGCA'G
Cuts at: 830 2115 2124 2319 3153 3240 3320 830
Size: 1285 9 195 834 87 80 3478
Fragments arranged by size:
3478 1285 834 195 87 80 9
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5968
PvuII CAG'CTG
Cuts at: 2752 2752
Size: 5968
SacII CC_GC'GG
Cuts at: 2864 2864
Size: 5968
SalI G'TCGA_C
Cuts at: 1936 3310 1936
Size: 1374 4594
SanDI GG'GwC_CC
Cuts at: 2220 2220
Size: 5968
SbfI CC_TGCA'GG
Cuts at: 3240 3320 3240
Size: 80 5888
ScaI AGT'ACT
Cuts at: 4216 5928 4216
Size: 1712 4256
SexAI A'CCwGG_T
Cuts at: 2193 2193
Size: 5968
SfcI C'TryA_G
Cuts at: 826 1045 2111 2120 2315 3149 3236 3316 4451
Size: 219 1066 9 195 834 87 80 1135
Cuts at: 4451 5132 5323 826
Size: 681 191 1471
Fragments arranged by size:
1471 1135 1066 834 681 219 195 191
87 80 9
SfiI GGCCn_nnn'nGGCC
Cuts at: 2408 2417 2408
Size: 9 5959
SfoI GGC'GCC
Cuts at: 2214 2214
Size: 5968
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5968
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5968
SmaI CCC'GGG
Cuts at: 1872 2166 2218 2310 2714 3295 1872
Size: 294 52 92 404 581 4545
Fragments arranged by size:
4545 581 404 294 92 52
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4072 4973 5235 820
Size: 197 926 2129 901 262 1553
Fragments arranged by size:
2129 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3759 493
Size: 3266 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5968
SphI G_CATG'C
Cuts at: 1132 3326 1132
Size: 2194 3774
SspI AAT'ATT
Cuts at: 5 52 3892 5
Size: 47 3840 2081
Fragments arranged by size:
3840 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5968
StyI C'CwwG_G
Cuts at: 513 1065 2004 2145 2853 3407 513
Size: 552 939 141 708 554 3074
Fragments arranged by size:
3074 939 708 554 552 141
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2682 2966 3995 4012 4165 4350 4795
Size: 1255 1102 284 1029 17 153 185 445
Cuts at: 4795 325
Size: 1498
Fragments arranged by size:
1498 1255 1102 1029 445 284 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4214 5926 96
Size: 274 80 33 51 1443 2237 1712 138
Fragments arranged by size:
2237 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5928 5960 5928
Size: 32 5936
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5968
XbaI T'CTAG_A
Cuts at: 3304 3304
Size: 5968
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5968
XmaI C'CCGG_G
Cuts at: 1870 2164 2216 2308 2712 3293 1870
Size: 294 52 92 404 581 4545
Fragments arranged by size:
4545 581 404 294 92 52
XmnI GAAnn'nnTTC
Cuts at: 4097 4097
Size: 5968
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3776 276
Size: 53 83 186 936 2242 2468
Fragments arranged by size:
2468 2242 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI
PvuI PvuII SacII SalI SanDI SbfI ScaI SexAI
SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbvCI BfrBI
Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PfoI PspOMI PspXI PsrI
RsrII SacI SapI SrfI SwaI XcmI